Click here to switch to the map view.

The map label for this gene is smpB

Identifier: 118579742

GI number: 118579742

Start: 1386415

End: 1386873

Strand: Direct

Name: smpB

Synonym: Ppro_1314

Alternate gene names: 118579742

Gene position: 1386415-1386873 (Clockwise)

Preceding gene: 118579741

Following gene: 118579743

Centisome position: 34.59

GC content: 50.54

Gene sequence:

>459_bases
ATGGGTGAAAAACTGATCTGTAATAACAAAAAGGCCTACCACGACTATTTCATCGAGGAAAAGATGGAGGCCGGTCTTGT
CCTGCTGGGAACCGAGGTGAAGTCCTTGCGGGCTGGCAAAGCCAACCTGAACGACTCCTTCATGCTGGTGCGCGATGGCG
AAGCCTTTCTGCATAATCTGCACATCTCTCCCTACGAGTTCGGCAATCGCCAGAACCATCAGGCCGATCGCAATAGAAAA
CTGCTCTTGCACCGCAAGCAGATTGATCGTTTGTACGGCAGAATCAGGGAACAGGGCTATTCGATCGTTCCCCTGCGCAT
GTATTTCAAGGACGGTCTGGTGAAGGTCGAGATAGGGCTTGCCAAAGGCAAGAAACTATACGATAAACGTGAAGACATGA
AGAAGAAGGACAGTCAGCGCGAACTGTCCCAGGCGCTCAAATCGAAAAACAGGGAGTAA

Upstream 100 bases:

>100_bases
TTATCGCATATTTCAAACGGGAGTCCATTCACGGATTCCCGTTTGCTGTTCTCCCAATCTGGTCGTTCCCTTTCTTCGGT
AAATCTGTTAGGATCGCGCA

Downstream 100 bases:

>100_bases
GGCCGAATACGCTGGACCAAGAACGAGTAATAATCACGAAATTACCGGTTTCTGGTCACTGGTCACGGAATTTAAGGGGG
TGTAAAGGTTTCGACGGGGA

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 152; Mature: 151

Protein sequence:

>152_residues
MGEKLICNNKKAYHDYFIEEKMEAGLVLLGTEVKSLRAGKANLNDSFMLVRDGEAFLHNLHISPYEFGNRQNHQADRNRK
LLLHRKQIDRLYGRIREQGYSIVPLRMYFKDGLVKVEIGLAKGKKLYDKREDMKKKDSQRELSQALKSKNRE

Sequences:

>Translated_152_residues
MGEKLICNNKKAYHDYFIEEKMEAGLVLLGTEVKSLRAGKANLNDSFMLVRDGEAFLHNLHISPYEFGNRQNHQADRNRK
LLLHRKQIDRLYGRIREQGYSIVPLRMYFKDGLVKVEIGLAKGKKLYDKREDMKKKDSQRELSQALKSKNRE
>Mature_151_residues
GEKLICNNKKAYHDYFIEEKMEAGLVLLGTEVKSLRAGKANLNDSFMLVRDGEAFLHNLHISPYEFGNRQNHQADRNRKL
LLHRKQIDRLYGRIREQGYSIVPLRMYFKDGLVKVEIGLAKGKKLYDKREDMKKKDSQRELSQALKSKNRE

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=151, Percent_Identity=49.0066225165563, Blast_Score=152, Evalue=6e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_PELPD (A1ANL4)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_900992.1
- ProteinModelPortal:   A1ANL4
- SMR:   A1ANL4
- STRING:   A1ANL4
- GeneID:   4572362
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_1314
- eggNOG:   COG0691
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- PhylomeDB:   A1ANL4
- ProtClustDB:   PRK05422
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 17826; Mature: 17695

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEKLICNNKKAYHDYFIEEKMEAGLVLLGTEVKSLRAGKANLNDSFMLVRDGEAFLHNL
CCCCEEECCCHHHHHHHHHHHHHCCEEEEECCHHHHHCCCCCCCCCEEEEECCHHHHHHC
HISPYEFGNRQNHQADRNRKLLLHRKQIDRLYGRIREQGYSIVPLRMYFKDGLVKVEIGL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEEEEC
AKGKKLYDKREDMKKKDSQRELSQALKSKNRE
CCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GEKLICNNKKAYHDYFIEEKMEAGLVLLGTEVKSLRAGKANLNDSFMLVRDGEAFLHNL
CCCEEECCCHHHHHHHHHHHHHCCEEEEECCHHHHHCCCCCCCCCEEEEECCHHHHHHC
HISPYEFGNRQNHQADRNRKLLLHRKQIDRLYGRIREQGYSIVPLRMYFKDGLVKVEIGL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEEEEC
AKGKKLYDKREDMKKKDSQRELSQALKSKNRE
CCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA