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The map label for this gene is trpB2 [H]

Identifier: 118579721

GI number: 118579721

Start: 1362172

End: 1363527

Strand: Reverse

Name: trpB2 [H]

Synonym: Ppro_1293

Alternate gene names: 118579721

Gene position: 1363527-1362172 (Counterclockwise)

Preceding gene: 118579722

Following gene: 118579720

Centisome position: 34.02

GC content: 63.2

Gene sequence:

>1356_bases
GTGAACACAAAAATTCTACTCAACGAAGATCAGATTCCCCGCCAGTGGTACAACATCATCCCCGATATGCCAGGCCCGCT
GGCGCCGGTCATCAACCCCCAGACATTGAAGCCTATCGTTCCGGAGGACATGCTGTCGATCTTCCCCATGTCCTTGATCG
AACAGGAGATGTCCCCCCAGCGCTGGATCGACATCCCGGACGAGGTGCGTGACATATACAGACTCTGGCGGCCGTCACCG
CTGTTCCGTGCCCACCGCCTGGAAAAGGCGCTGGGGACACCGGCCAAGATCTACTACAAGCACGAGGGGGTCTCGCCGGC
CGGTTCCCACAAGCCCAACTCGGCAATCCCCCAGGCCTGGTACAACAAGCAGGCCGGCATCCGCCGCCTGGCCACGGAGA
CCGGGGCCGGACAGTGGGGCACGTCACTGGCCCTGGCCTGCTCCATGTTCGGCCTGGAGTGCACCATCTACATGGTCAAG
GTATCCTGCAGCCAGAAACCGTACCGCAAGAGCATGATGCAGCTGTGGGGGGCAACGGTGATCCCCTCCCCTTCCGAATT
AACCAATGCCGGGCGCTCGATCCTGGCCCACGATCCGGACTGCAGCGGCAGCCTGGGCATCGCCATCTCCGAGGCGGTGG
AGGATGCGGCCAACCATGCCGACACCAACTACGCCCTGGGTAGCGTGCTCAACCATGTCTGCCTGCACCAGACCGTGATC
GGCCAGGAGGCCAGGGAGCAGATGAAGATTGCCGGTGACTACCCGGACGTGGTCATCGCCTGCTGCGGCGGCGGCTCCAA
TTTTGCCGGCCTGGCCTTCCCCTTCATCGCCGACCGGGCCAGTGGCAAGCAGGTGCGCTGTCTTGCGGTTGAGCCGGCGT
CCTGCCCGACCCTCACCAAGGGGGTCTATGCCTTTGACTACGGCGACACCGCCAGGATGGCGCCCATCGCCATGATGTAT
ACTCTGGGCCATGACTTCGTCCCCCCCGGCATTCATGCCGGCGGTCTGCGTTACCACGGTCAGTCCCCTCTGATCTCCCA
GCTCTACCACGCCGGCCAGATCGAGGCCAAGTCTTACAAGCAGAATGCCTGCTTCGAGGGGGCACTGCTCTTCTCCCGCT
GCGAGGGGATCGTGCCTGCGCCCGAGTCGTCCCATGCGGTGCGCGCTGCCATCGACGAGGCGGTGCTGGCCAAGGAAGAG
GGCAAGGAGCGCACCATCCTGTTCGGCCTGTCCGGGCACGGCCAGCTGGACATGGGCGCCTACGACGCCTACCTCTCCGG
CGACCTGGAAGATTACGAGTATTCAAGCGAGCTGGTGAAACAGTCACTGGAGCGTCTGCCCAAAGTACAGCTGTAA

Upstream 100 bases:

>100_bases
TGGAGGAGATCGACCGACTCGACTTCGTCTGCGAAAAGACCAGTTTTATCAGGATCGAGGACAATCTGGAATAATATGAA
ATAATCCTGGAGCGACTTAA

Downstream 100 bases:

>100_bases
CCGGAACCGGGGATCCGTCTCTTTTTTACGGGTCCCCGGTCTCGCCTCACCGGTCCTATCATACATGATCAAAGTAAAAA
TCTGCGGTATAACCAACCTG

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MNTKILLNEDQIPRQWYNIIPDMPGPLAPVINPQTLKPIVPEDMLSIFPMSLIEQEMSPQRWIDIPDEVRDIYRLWRPSP
LFRAHRLEKALGTPAKIYYKHEGVSPAGSHKPNSAIPQAWYNKQAGIRRLATETGAGQWGTSLALACSMFGLECTIYMVK
VSCSQKPYRKSMMQLWGATVIPSPSELTNAGRSILAHDPDCSGSLGIAISEAVEDAANHADTNYALGSVLNHVCLHQTVI
GQEAREQMKIAGDYPDVVIACCGGGSNFAGLAFPFIADRASGKQVRCLAVEPASCPTLTKGVYAFDYGDTARMAPIAMMY
TLGHDFVPPGIHAGGLRYHGQSPLISQLYHAGQIEAKSYKQNACFEGALLFSRCEGIVPAPESSHAVRAAIDEAVLAKEE
GKERTILFGLSGHGQLDMGAYDAYLSGDLEDYEYSSELVKQSLERLPKVQL

Sequences:

>Translated_451_residues
MNTKILLNEDQIPRQWYNIIPDMPGPLAPVINPQTLKPIVPEDMLSIFPMSLIEQEMSPQRWIDIPDEVRDIYRLWRPSP
LFRAHRLEKALGTPAKIYYKHEGVSPAGSHKPNSAIPQAWYNKQAGIRRLATETGAGQWGTSLALACSMFGLECTIYMVK
VSCSQKPYRKSMMQLWGATVIPSPSELTNAGRSILAHDPDCSGSLGIAISEAVEDAANHADTNYALGSVLNHVCLHQTVI
GQEAREQMKIAGDYPDVVIACCGGGSNFAGLAFPFIADRASGKQVRCLAVEPASCPTLTKGVYAFDYGDTARMAPIAMMY
TLGHDFVPPGIHAGGLRYHGQSPLISQLYHAGQIEAKSYKQNACFEGALLFSRCEGIVPAPESSHAVRAAIDEAVLAKEE
GKERTILFGLSGHGQLDMGAYDAYLSGDLEDYEYSSELVKQSLERLPKVQL
>Mature_451_residues
MNTKILLNEDQIPRQWYNIIPDMPGPLAPVINPQTLKPIVPEDMLSIFPMSLIEQEMSPQRWIDIPDEVRDIYRLWRPSP
LFRAHRLEKALGTPAKIYYKHEGVSPAGSHKPNSAIPQAWYNKQAGIRRLATETGAGQWGTSLALACSMFGLECTIYMVK
VSCSQKPYRKSMMQLWGATVIPSPSELTNAGRSILAHDPDCSGSLGIAISEAVEDAANHADTNYALGSVLNHVCLHQTVI
GQEAREQMKIAGDYPDVVIACCGGGSNFAGLAFPFIADRASGKQVRCLAVEPASCPTLTKGVYAFDYGDTARMAPIAMMY
TLGHDFVPPGIHAGGLRYHGQSPLISQLYHAGQIEAKSYKQNACFEGALLFSRCEGIVPAPESSHAVRAAIDEAVLAKEE
GKERTILFGLSGHGQLDMGAYDAYLSGDLEDYEYSSELVKQSLERLPKVQL

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG1350

COG function: function code R; Predicted alternative tryptophan synthase beta-subunit (paralog of TrpB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=412, Percent_Identity=27.6699029126214, Blast_Score=119, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6321412, Length=384, Percent_Identity=31.7708333333333, Blast_Score=135, Evalue=2e-32,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006316
- InterPro:   IPR006653
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 49284; Mature: 49284

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTKILLNEDQIPRQWYNIIPDMPGPLAPVINPQTLKPIVPEDMLSIFPMSLIEQEMSPQ
CCCEEEECCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
RWIDIPDEVRDIYRLWRPSPLFRAHRLEKALGTPAKIYYKHEGVSPAGSHKPNSAIPQAW
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCHHH
YNKQAGIRRLATETGAGQWGTSLALACSMFGLECTIYMVKVSCSQKPYRKSMMQLWGATV
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCEEEEEEEEEECCCCHHHHHHHHHHCCCC
IPSPSELTNAGRSILAHDPDCSGSLGIAISEAVEDAANHADTNYALGSVLNHVCLHQTVI
CCCCHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
GQEAREQMKIAGDYPDVVIACCGGGSNFAGLAFPFIADRASGKQVRCLAVEPASCPTLTK
HHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCHHC
GVYAFDYGDTARMAPIAMMYTLGHDFVPPGIHAGGLRYHGQSPLISQLYHAGQIEAKSYK
CEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCHHHHHHHHCCCCCHHHHH
QNACFEGALLFSRCEGIVPAPESSHAVRAAIDEAVLAKEEGKERTILFGLSGHGQLDMGA
CCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCH
YDAYLSGDLEDYEYSSELVKQSLERLPKVQL
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNTKILLNEDQIPRQWYNIIPDMPGPLAPVINPQTLKPIVPEDMLSIFPMSLIEQEMSPQ
CCCEEEECCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
RWIDIPDEVRDIYRLWRPSPLFRAHRLEKALGTPAKIYYKHEGVSPAGSHKPNSAIPQAW
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCHHH
YNKQAGIRRLATETGAGQWGTSLALACSMFGLECTIYMVKVSCSQKPYRKSMMQLWGATV
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCEEEEEEEEEECCCCHHHHHHHHHHCCCC
IPSPSELTNAGRSILAHDPDCSGSLGIAISEAVEDAANHADTNYALGSVLNHVCLHQTVI
CCCCHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
GQEAREQMKIAGDYPDVVIACCGGGSNFAGLAFPFIADRASGKQVRCLAVEPASCPTLTK
HHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCHHC
GVYAFDYGDTARMAPIAMMYTLGHDFVPPGIHAGGLRYHGQSPLISQLYHAGQIEAKSYK
CEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCHHHHHHHHCCCCCHHHHH
QNACFEGALLFSRCEGIVPAPESSHAVRAAIDEAVLAKEEGKERTILFGLSGHGQLDMGA
CCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCH
YDAYLSGDLEDYEYSSELVKQSLERLPKVQL
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]