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The map label for this gene is bioB [H]

Identifier: 118579607

GI number: 118579607

Start: 1229658

End: 1230935

Strand: Direct

Name: bioB [H]

Synonym: Ppro_1177

Alternate gene names: 118579607

Gene position: 1229658-1230935 (Clockwise)

Preceding gene: 118579606

Following gene: 118579608

Centisome position: 30.68

GC content: 61.19

Gene sequence:

>1278_bases
ATGACCGAGCAGGAACTTCTCGATTTCGACAGGGCGCACATTTGGCACCCCTATACCTCGGCCACAAAGGCTCTAACCTG
TTATCCGGTGGAGCAGGCCGAGGGGGTGTATATCCAGCTGATGGATGGGCGGCGCCTGATCGACGGCATGTCCAGCTGGT
GGTCCGCCATTCACGGCTACAACCACCCGGTGATCAACGCCGCCATGACAGCCCAGATCGGGCGCATGTCCCACGTCATG
TTCGGCGGGCTGACCCACGGGCCTGCTGTGGAGTTGGCAAAGCTCCTGGTCAGCATCGTCCCCGCCGGCCTTGAACATGT
CTTTTTCAGCGACTCCGGCTCGGTAGCCGTGGAGGTGGCCATGAAGATGGCCCTCCAGTACTGGCATTCCCGCTCATGCG
TCACGAAGCGCTCGTTTGTTACCGTCCGAAACGGCTATCACGGCGACACCTGGCACGCCATGTCCGTATCCGACCCGGAC
AGCGGAATGCACGCCATCTTCAACACTAACCTGGCGCAGCAGTTCTTTCTGCCTGCGCCGCGCTCAGGCTTCCACGACCC
GTTCGATGAAACGGAAATCCAGCAGGTGGAGGCATTCTTAAGCCGGCACCATGAAAGCATTGCCGCCTTTATCCTCGAAC
CGATTGTGCAGGGCGCAGGCGGCATGCGCTTCTATCATCCCGGCTACCTGAAGCGGGTACGCGAGCTGTGCACGAAATAC
CGCCTGCTGCTCATCGCCGACGAAATTGCGACCGGTTTCGGCCGCAGCGGAAAGCTGTTCGCCTGTGAGCATGCCGCCAT
TTCGCCGGATATTATCTGCGTGGGCAAGGCCTTGACGGGCGGTTACCTCTCCTTTGCCGCCACGCTCTGTACCCGGGACG
TGGCCCATACCATTTCCGACGGTTCGCCCGGCGTGTTCATGCACGGCCCGACGTTCATGGCCAATCCCCTGGCCTGCGCC
ACCGCCCTGGCCAGCACCCGGCTCCTCTTGAGCGGCAACTGGCAGGAACGGGTCCTGTGCATCGAGAAGACGTTGCAAGA
ACAGCTGCTGCCATTGCAACAGGCTGATGCGGTTGCCGACGTCCGCGTCCTGGGGGCCATCGGTGTGGTGGAGATGAAAG
AGCCGGTACAGATGGAACGGATTCAGCAGCGCTTTGTGGATCAGGGGGTCTGGCTGCGGCCGTTCGGCAGGCTGGTCTAT
ACCATGCCGCCCTTTATCATCTCCGATGACGAGCTGCACGCCCTCTGCAGGGGGATAACCAACGTAATCGCAGGATGA

Upstream 100 bases:

>100_bases
ACAGCCGGGAACCGGTGACCTGCTGCGAAAGCTGGGGGTGGGGCAGGCGACTGCCTGAAGGGCTGCCAGGGAACGCTATT
TTTGGGGGGATGCTGAAATA

Downstream 100 bases:

>100_bases
AACCCCGGCGTCCGGGCCGATCATTCTCCGAGAACCCCTTCTTTCGAGGCGTCCCCCCTTCCCGTGCGGCCCCAGCCCCC
GTGCCCTGCTCATTCCCCCA

Product: adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MTEQELLDFDRAHIWHPYTSATKALTCYPVEQAEGVYIQLMDGRRLIDGMSSWWSAIHGYNHPVINAAMTAQIGRMSHVM
FGGLTHGPAVELAKLLVSIVPAGLEHVFFSDSGSVAVEVAMKMALQYWHSRSCVTKRSFVTVRNGYHGDTWHAMSVSDPD
SGMHAIFNTNLAQQFFLPAPRSGFHDPFDETEIQQVEAFLSRHHESIAAFILEPIVQGAGGMRFYHPGYLKRVRELCTKY
RLLLIADEIATGFGRSGKLFACEHAAISPDIICVGKALTGGYLSFAATLCTRDVAHTISDGSPGVFMHGPTFMANPLACA
TALASTRLLLSGNWQERVLCIEKTLQEQLLPLQQADAVADVRVLGAIGVVEMKEPVQMERIQQRFVDQGVWLRPFGRLVY
TMPPFIISDDELHALCRGITNVIAG

Sequences:

>Translated_425_residues
MTEQELLDFDRAHIWHPYTSATKALTCYPVEQAEGVYIQLMDGRRLIDGMSSWWSAIHGYNHPVINAAMTAQIGRMSHVM
FGGLTHGPAVELAKLLVSIVPAGLEHVFFSDSGSVAVEVAMKMALQYWHSRSCVTKRSFVTVRNGYHGDTWHAMSVSDPD
SGMHAIFNTNLAQQFFLPAPRSGFHDPFDETEIQQVEAFLSRHHESIAAFILEPIVQGAGGMRFYHPGYLKRVRELCTKY
RLLLIADEIATGFGRSGKLFACEHAAISPDIICVGKALTGGYLSFAATLCTRDVAHTISDGSPGVFMHGPTFMANPLACA
TALASTRLLLSGNWQERVLCIEKTLQEQLLPLQQADAVADVRVLGAIGVVEMKEPVQMERIQQRFVDQGVWLRPFGRLVY
TMPPFIISDDELHALCRGITNVIAG
>Mature_424_residues
TEQELLDFDRAHIWHPYTSATKALTCYPVEQAEGVYIQLMDGRRLIDGMSSWWSAIHGYNHPVINAAMTAQIGRMSHVMF
GGLTHGPAVELAKLLVSIVPAGLEHVFFSDSGSVAVEVAMKMALQYWHSRSCVTKRSFVTVRNGYHGDTWHAMSVSDPDS
GMHAIFNTNLAQQFFLPAPRSGFHDPFDETEIQQVEAFLSRHHESIAAFILEPIVQGAGGMRFYHPGYLKRVRELCTKYR
LLLIADEIATGFGRSGKLFACEHAAISPDIICVGKALTGGYLSFAATLCTRDVAHTISDGSPGVFMHGPTFMANPLACAT
ALASTRLLLSGNWQERVLCIEKTLQEQLLPLQQADAVADVRVLGAIGVVEMKEPVQMERIQQRFVDQGVWLRPFGRLVYT
MPPFIISDDELHALCRGITNVIAG

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=424, Percent_Identity=29.7169811320755, Blast_Score=165, Evalue=9e-41,
Organism=Homo sapiens, GI284507298, Length=319, Percent_Identity=32.9153605015674, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI13994255, Length=376, Percent_Identity=28.1914893617021, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI226442705, Length=386, Percent_Identity=24.8704663212435, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI37574042, Length=437, Percent_Identity=23.7986270022883, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI226442709, Length=380, Percent_Identity=25, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI24119277, Length=360, Percent_Identity=26.3888888888889, Blast_Score=79, Evalue=7e-15,
Organism=Escherichia coli, GI1786991, Length=417, Percent_Identity=60.1918465227818, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI145693181, Length=341, Percent_Identity=32.8445747800587, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI1789016, Length=406, Percent_Identity=26.8472906403941, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1789759, Length=343, Percent_Identity=30.6122448979592, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1788044, Length=391, Percent_Identity=30.690537084399, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI1787560, Length=408, Percent_Identity=26.7156862745098, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1786349, Length=383, Percent_Identity=27.6762402088773, Blast_Score=99, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI25144271, Length=421, Percent_Identity=28.0285035629454, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI25144274, Length=277, Percent_Identity=32.8519855595668, Blast_Score=128, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI32564660, Length=423, Percent_Identity=26.7139479905437, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI71992977, Length=424, Percent_Identity=22.1698113207547, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17541228, Length=290, Percent_Identity=27.2413793103448, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324386, Length=459, Percent_Identity=44.0087145969499, Blast_Score=382, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6323470, Length=430, Percent_Identity=28.1395348837209, Blast_Score=138, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6324432, Length=383, Percent_Identity=25.5874673629243, Blast_Score=100, Evalue=8e-22,
Organism=Drosophila melanogaster, GI21357415, Length=410, Percent_Identity=30, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI28574759, Length=405, Percent_Identity=27.1604938271605, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI161085790, Length=409, Percent_Identity=26.6503667481663, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21356575, Length=371, Percent_Identity=25.6064690026954, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR005814
- InterPro:   IPR005815
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR007197 [H]

Pfam domain/function: PF00202 Aminotran_3; PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 46961; Mature: 46829

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQELLDFDRAHIWHPYTSATKALTCYPVEQAEGVYIQLMDGRRLIDGMSSWWSAIHGY
CCHHHHHHCCHHHEECCCCCCCCEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHCCC
NHPVINAAMTAQIGRMSHVMFGGLTHGPAVELAKLLVSIVPAGLEHVFFSDSGSVAVEVA
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHEEEECCCCCHHHHHH
MKMALQYWHSRSCVTKRSFVTVRNGYHGDTWHAMSVSDPDSGMHAIFNTNLAQQFFLPAP
HHHHHHHHHCCCCCCHHCEEEEECCCCCCCEEEEECCCCCCCCHHHHCCCHHHHHCCCCC
RSGFHDPFDETEIQQVEAFLSRHHESIAAFILEPIVQGAGGMRFYHPGYLKRVRELCTKY
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHH
RLLLIADEIATGFGRSGKLFACEHAAISPDIICVGKALTGGYLSFAATLCTRDVAHTISD
HHHEEEHHHHHCCCCCCCEEEECCCCCCCCEEEECCHHCCHHHHHHHHHHHHHHHHHHCC
GSPGVFMHGPTFMANPLACATALASTRLLLSGNWQERVLCIEKTLQEQLLPLQQADAVAD
CCCCEEEECCCHHCCHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHH
VRVLGAIGVVEMKEPVQMERIQQRFVDQGVWLRPFGRLVYTMPPFIISDDELHALCRGIT
HHHHHHCCCEECCCCHHHHHHHHHHHHCCCEECHHHHHHHCCCCEEECCHHHHHHHHHHH
NVIAG
HHHCC
>Mature Secondary Structure 
TEQELLDFDRAHIWHPYTSATKALTCYPVEQAEGVYIQLMDGRRLIDGMSSWWSAIHGY
CHHHHHHCCHHHEECCCCCCCCEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHCCC
NHPVINAAMTAQIGRMSHVMFGGLTHGPAVELAKLLVSIVPAGLEHVFFSDSGSVAVEVA
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHEEEECCCCCHHHHHH
MKMALQYWHSRSCVTKRSFVTVRNGYHGDTWHAMSVSDPDSGMHAIFNTNLAQQFFLPAP
HHHHHHHHHCCCCCCHHCEEEEECCCCCCCEEEEECCCCCCCCHHHHCCCHHHHHCCCCC
RSGFHDPFDETEIQQVEAFLSRHHESIAAFILEPIVQGAGGMRFYHPGYLKRVRELCTKY
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHH
RLLLIADEIATGFGRSGKLFACEHAAISPDIICVGKALTGGYLSFAATLCTRDVAHTISD
HHHEEEHHHHHCCCCCCCEEEECCCCCCCCEEEECCHHCCHHHHHHHHHHHHHHHHHHCC
GSPGVFMHGPTFMANPLACATALASTRLLLSGNWQERVLCIEKTLQEQLLPLQQADAVAD
CCCCEEEECCCHHCCHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHH
VRVLGAIGVVEMKEPVQMERIQQRFVDQGVWLRPFGRLVYTMPPFIISDDELHALCRGIT
HHHHHHCCCEECCCCHHHHHHHHHHHHCCCEECHHHHHHHCCCCEEECCHHHHHHHHHHH
NVIAG
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA