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The map label for this gene is truB [H]

Identifier: 118579402

GI number: 118579402

Start: 1009220

End: 1010134

Strand: Direct

Name: truB [H]

Synonym: Ppro_0966

Alternate gene names: 118579402

Gene position: 1009220-1010134 (Clockwise)

Preceding gene: 118579401

Following gene: 118579403

Centisome position: 25.18

GC content: 62.51

Gene sequence:

>915_bases
GTGATTAGCGGCTTCTTCGTTATCGATAAACCTGCCGGCATCAGCTCCCACGACGTGGTCAGCCGTGTGCGCAGAATCCT
GGGTACACGCAGGGTGGGGCACACCGGTACGCTCGACCCTTTTGCAACCGGTGTTCTGCCGGTGGCGGTCAATGACGCCA
CCAAGGTGATCCCTTTTCTGGACGAGGGGGTCAAATGCTACCAGGCCCTGCTGCGCCTGGGCGTGGTGACCGATACCCTG
GACATGACCGGCAGGATTCTTGCCGAGGGAGATTACTCCTCCGTCAGCCGGGAGCGCTTCCTTGAGCTGTTGACCCGCTT
CTGTGGGGTGATTAGCCAGGTTCCCCCCATGTTCTCGGCAATCAAGCGTGACGGTCAGCCGCTGTACAAGCTGGCCAGAC
AGGGCATCGAGGTGGAGCGCCGGGCGCGACAGGTGGAGATTCACTCCCTTGAGCTTTTGTCGTTTGAGCCGCCTCTGGCA
TGCCTGCGGGTCAGCTGCTCCCGCGGCACCTATGTGCGTAGCTTGGCGGATGACCTTGGAGCGGAGTTGGGGTGCGGAGC
GTCCTTGCAGGAACTGCGCAGAATTGCCAGCGGACCGTTCCATCTCTCCAGCGCCATTACGCTTGCGACGCTTGAGCAGG
CCAAGGAGGGAGGGATGTCGGATGCGCTCTGTCTGGATCCCTATGCGGCCCTTGCGCATCTGCCCGACATTCCCCTGTCC
GATGCCGGACTGGCCAAGGTCAGGCATGGCAGGTCGCCGGAATGGGGGGAGACGCTGCTTTCCGCTCCCTGCGAGGGGAC
TGAGGCAGGGCTGGCTCGGCTCTCACGTAACGGGAGTCTGGCCGCCGTTGCCCGGATCGAACCGCTGCCTTATGGTGGTT
CCCGGCTGGTTCTCAAGCGGGTATTCTGCAATTAG

Upstream 100 bases:

>100_bases
CCCTGGGTGGTGGCGGACACCACAACGCCGCCGGCTGCACGGTCTCCGGCGGTCTGGAAGAGATCAAGTCCCGCGTCTAC
GACATTGTGAACGCCTCCCT

Downstream 100 bases:

>100_bases
TTAGTGCTTTACATGGCAATGGCGGTGTGCTATTAGTCTCAGACTGCGGTAATGCTGAATAAATACGTGTCATCAGGACA
ATAAACCGAACATGAAGGGG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MISGFFVIDKPAGISSHDVVSRVRRILGTRRVGHTGTLDPFATGVLPVAVNDATKVIPFLDEGVKCYQALLRLGVVTDTL
DMTGRILAEGDYSSVSRERFLELLTRFCGVISQVPPMFSAIKRDGQPLYKLARQGIEVERRARQVEIHSLELLSFEPPLA
CLRVSCSRGTYVRSLADDLGAELGCGASLQELRRIASGPFHLSSAITLATLEQAKEGGMSDALCLDPYAALAHLPDIPLS
DAGLAKVRHGRSPEWGETLLSAPCEGTEAGLARLSRNGSLAAVARIEPLPYGGSRLVLKRVFCN

Sequences:

>Translated_304_residues
MISGFFVIDKPAGISSHDVVSRVRRILGTRRVGHTGTLDPFATGVLPVAVNDATKVIPFLDEGVKCYQALLRLGVVTDTL
DMTGRILAEGDYSSVSRERFLELLTRFCGVISQVPPMFSAIKRDGQPLYKLARQGIEVERRARQVEIHSLELLSFEPPLA
CLRVSCSRGTYVRSLADDLGAELGCGASLQELRRIASGPFHLSSAITLATLEQAKEGGMSDALCLDPYAALAHLPDIPLS
DAGLAKVRHGRSPEWGETLLSAPCEGTEAGLARLSRNGSLAAVARIEPLPYGGSRLVLKRVFCN
>Mature_304_residues
MISGFFVIDKPAGISSHDVVSRVRRILGTRRVGHTGTLDPFATGVLPVAVNDATKVIPFLDEGVKCYQALLRLGVVTDTL
DMTGRILAEGDYSSVSRERFLELLTRFCGVISQVPPMFSAIKRDGQPLYKLARQGIEVERRARQVEIHSLELLSFEPPLA
CLRVSCSRGTYVRSLADDLGAELGCGASLQELRRIASGPFHLSSAITLATLEQAKEGGMSDALCLDPYAALAHLPDIPLS
DAGLAKVRHGRSPEWGETLLSAPCEGTEAGLARLSRNGSLAAVARIEPLPYGGSRLVLKRVFCN

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=218, Percent_Identity=39.9082568807339, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI4503337, Length=198, Percent_Identity=34.3434343434343, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI215599015, Length=198, Percent_Identity=33.8383838383838, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI2367200, Length=230, Percent_Identity=42.6086956521739, Blast_Score=182, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17553978, Length=199, Percent_Identity=32.1608040201005, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323204, Length=210, Percent_Identity=34.2857142857143, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6324037, Length=193, Percent_Identity=28.4974093264249, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281364189, Length=200, Percent_Identity=32.5, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI281364187, Length=200, Percent_Identity=32.5, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI281364185, Length=200, Percent_Identity=32.5, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI281364183, Length=200, Percent_Identity=32.5, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI62471759, Length=200, Percent_Identity=32.5, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI17975520, Length=200, Percent_Identity=32.5, Blast_Score=99, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 32766; Mature: 32766

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISGFFVIDKPAGISSHDVVSRVRRILGTRRVGHTGTLDPFATGVLPVAVNDATKVIPFL
CCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCEEEEECCHHHHHHHH
DEGVKCYQALLRLGVVTDTLDMTGRILAEGDYSSVSRERFLELLTRFCGVISQVPPMFSA
HHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
IKRDGQPLYKLARQGIEVERRARQVEIHSLELLSFEPPLACLRVSCSRGTYVRSLADDLG
HHHCCHHHHHHHHCCCHHHHHHHHHHHHEEEEECCCCCHHHHEECCCCCHHHHHHHHHHC
AELGCGASLQELRRIASGPFHLSSAITLATLEQAKEGGMSDALCLDPYAALAHLPDIPLS
CCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCHHHHHHHCCCCCCC
DAGLAKVRHGRSPEWGETLLSAPCEGTEAGLARLSRNGSLAAVARIEPLPYGGSRLVLKR
CCCHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHH
VFCN
HHCC
>Mature Secondary Structure
MISGFFVIDKPAGISSHDVVSRVRRILGTRRVGHTGTLDPFATGVLPVAVNDATKVIPFL
CCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCEEEEECCHHHHHHHH
DEGVKCYQALLRLGVVTDTLDMTGRILAEGDYSSVSRERFLELLTRFCGVISQVPPMFSA
HHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
IKRDGQPLYKLARQGIEVERRARQVEIHSLELLSFEPPLACLRVSCSRGTYVRSLADDLG
HHHCCHHHHHHHHCCCHHHHHHHHHHHHEEEEECCCCCHHHHEECCCCCHHHHHHHHHHC
AELGCGASLQELRRIASGPFHLSSAITLATLEQAKEGGMSDALCLDPYAALAHLPDIPLS
CCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCHHHHHHHCCCCCCC
DAGLAKVRHGRSPEWGETLLSAPCEGTEAGLARLSRNGSLAAVARIEPLPYGGSRLVLKR
CCCHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHH
VFCN
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA