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The map label for this gene is ntrC [H]

Identifier: 118579312

GI number: 118579312

Start: 919783

End: 920523

Strand: Reverse

Name: ntrC [H]

Synonym: Ppro_0875

Alternate gene names: 118579312

Gene position: 920523-919783 (Counterclockwise)

Preceding gene: 118579318

Following gene: 118579310

Centisome position: 22.97

GC content: 53.98

Gene sequence:

>741_bases
ATGCACAACCACAAAAGCATCTTCATCGCCGACAGGGACCGAAAAAGCCGCGATCTCGTATCGGCTTTCTTAACCTACCA
GGGGTACGACGTTATCACGGGAGAAGACGCACCTTCTCTCTATACGGCCATCCAGACACAGCAGGTCGGCATAGTCATTG
CCGAACTGTCCCATCTCAACGCCATCGCTCCCGAATTCAGGGATCGTGCCAAAGAGACCAATCCCCTCCTGGTACTTGTT
ATCTACGGAGCAAGCGAGGAGTTGCCTCCACGGCAACCCGGCAAAGGAAATGTTTTCTTTCTTTCCAAGCCGCTCAACCT
TGATGAACTGGAGAGCATTGTCATGCGCGCCGAAGAATATCAAGCCATGAGGCAGATGGAGGGCGAGGGGCACGGACATA
AACAGACCAATATTCTCTGTTCCTCGATCATCGGCAAAAGCAAACCAATGCAGGACCTGTTCGAGATGATCGAAAAGGTG
GCCGAATCCAACGCCACGGTCCTGATCCAGGGAGAGTCGGGAACCGGCAAGGAACTTGCAGCACGTGCCATACACCAGCT
TAGCGGCAGGAAGAATCAGAATTTCGTCCCCATAAACTGCGCGGCGATTCCTGAGGACCTGCTGGAGAGCGAGTTATTCG
GCCACGTCAAGGGTTCTTTTACCGGAGCGCATGCCAACCGCATCGGCCGTTTTGAAATGGCCGACAAGGGAACACTCTTC
CTGGGAGTCTGTCGGGCTTGA

Upstream 100 bases:

>100_bases
TTTACCCGAAAGTCATTTTATTGACTTTTTAGGACACTAACGATACATTAAGCAAACATTGTTAGCCCGGCACCTCCACC
CCGTTCAACACTGGAAAAAA

Downstream 100 bases:

>100_bases
CCTCAGGGGCGCCCTCTTTTGTGGCCAAGCCGATTAAAAAAGTTCCGTATGTGTTCGTCTTCCCACCGGCAACCTGGTTA
ATTCAGCCCTTTCAGCGATG

Product: two component sigma54 specific Fis family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MHNHKSIFIADRDRKSRDLVSAFLTYQGYDVITGEDAPSLYTAIQTQQVGIVIAELSHLNAIAPEFRDRAKETNPLLVLV
IYGASEELPPRQPGKGNVFFLSKPLNLDELESIVMRAEEYQAMRQMEGEGHGHKQTNILCSSIIGKSKPMQDLFEMIEKV
AESNATVLIQGESGTGKELAARAIHQLSGRKNQNFVPINCAAIPEDLLESELFGHVKGSFTGAHANRIGRFEMADKGTLF
LGVCRA

Sequences:

>Translated_246_residues
MHNHKSIFIADRDRKSRDLVSAFLTYQGYDVITGEDAPSLYTAIQTQQVGIVIAELSHLNAIAPEFRDRAKETNPLLVLV
IYGASEELPPRQPGKGNVFFLSKPLNLDELESIVMRAEEYQAMRQMEGEGHGHKQTNILCSSIIGKSKPMQDLFEMIEKV
AESNATVLIQGESGTGKELAARAIHQLSGRKNQNFVPINCAAIPEDLLESELFGHVKGSFTGAHANRIGRFEMADKGTLF
LGVCRA
>Mature_246_residues
MHNHKSIFIADRDRKSRDLVSAFLTYQGYDVITGEDAPSLYTAIQTQQVGIVIAELSHLNAIAPEFRDRAKETNPLLVLV
IYGASEELPPRQPGKGNVFFLSKPLNLDELESIVMRAEEYQAMRQMEGEGHGHKQTNILCSSIIGKSKPMQDLFEMIEKV
AESNATVLIQGESGTGKELAARAIHQLSGRKNQNFVPINCAAIPEDLLESELFGHVKGSFTGAHANRIGRFEMADKGTLF
LGVCRA

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=238, Percent_Identity=36.5546218487395, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1788550, Length=238, Percent_Identity=39.0756302521008, Blast_Score=140, Evalue=6e-35,
Organism=Escherichia coli, GI1790437, Length=257, Percent_Identity=36.9649805447471, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI1789087, Length=99, Percent_Identity=63.6363636363636, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI87082117, Length=141, Percent_Identity=49.645390070922, Blast_Score=129, Evalue=1e-31,
Organism=Escherichia coli, GI1788905, Length=242, Percent_Identity=35.1239669421488, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1789233, Length=100, Percent_Identity=58, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI87082152, Length=101, Percent_Identity=60.3960396039604, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI87081872, Length=101, Percent_Identity=48.5148514851485, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1786524, Length=116, Percent_Identity=46.551724137931, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1789828, Length=162, Percent_Identity=38.2716049382716, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787583, Length=101, Percent_Identity=42.5742574257426, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 27138; Mature: 27138

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNHKSIFIADRDRKSRDLVSAFLTYQGYDVITGEDAPSLYTAIQTQQVGIVIAELSHLN
CCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCEEEEEHHHHH
AIAPEFRDRAKETNPLLVLVIYGASEELPPRQPGKGNVFFLSKPLNLDELESIVMRAEEY
HHCHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHH
QAMRQMEGEGHGHKQTNILCSSIIGKSKPMQDLFEMIEKVAESNATVLIQGESGTGKELA
HHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHH
ARAIHQLSGRKNQNFVPINCAAIPEDLLESELFGHVKGSFTGAHANRIGRFEMADKGTLF
HHHHHHHCCCCCCCEEEEEHHHCCHHHHHHHHHHHHCCCCCCHHHHHCCCEEECCCCEEE
LGVCRA
EEEECC
>Mature Secondary Structure
MHNHKSIFIADRDRKSRDLVSAFLTYQGYDVITGEDAPSLYTAIQTQQVGIVIAELSHLN
CCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCEEEEEHHHHH
AIAPEFRDRAKETNPLLVLVIYGASEELPPRQPGKGNVFFLSKPLNLDELESIVMRAEEY
HHCHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHH
QAMRQMEGEGHGHKQTNILCSSIIGKSKPMQDLFEMIEKVAESNATVLIQGESGTGKELA
HHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHH
ARAIHQLSGRKNQNFVPINCAAIPEDLLESELFGHVKGSFTGAHANRIGRFEMADKGTLF
HHHHHHHCCCCCCCEEEEEHHHCCHHHHHHHHHHHHCCCCCCHHHHHCCCEEECCCCEEE
LGVCRA
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]