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The map label for this gene is outF [H]

Identifier: 118579254

GI number: 118579254

Start: 862099

End: 863343

Strand: Direct

Name: outF [H]

Synonym: Ppro_0816

Alternate gene names: 118579254

Gene position: 862099-863343 (Clockwise)

Preceding gene: 118579253

Following gene: 118579255

Centisome position: 21.51

GC content: 59.84

Gene sequence:

>1245_bases
ATGTCGCTCTATTCCTTTATCGGCCTTGATGGTCAAGGGCGGGAACTGCGGGAAACCATCGAGGCTGAAACCGAAAAGGA
TGTGGCGCGTCTTCTCCGAAACCGCTCCATTTTCGTCCTGAAAGTCTGGGAGGGGGGGGGAGCGAAACCGGGAAACGGTT
TCCGGGAATGGCTGGCACGGCTGAAACCGCTCCTGAGGCCGGGACAGTATGCTCCCGTGGGGGCGGGAGATCTGGTGATT
TTCTTCCGCCAGACGGCGATGATGCTGCGCGCCGGATATACCCTGGTCACCGCCCTGGACGCGAGCTCCGAGATGACGGC
CAAACTGCGTCTGCGTCGGACCATCAAAAAGATGTGCGATGAAATCAGAAAAGGGGGCAGTTTTTCAGGGACCCTGGCCG
GAGAAAAGAAGATCTTCACCCCCATGGTTTCCAACCTGATCGCCAGCGGAGAAAAGAGCGGCAACCTGGACGACATTCTG
GACCGGCTGGCGGACAGTATCGAACGGAGCAAGGATATCAAGCGTCAGCTGGTAGCGGCCATGTTCTACCCCCTGTTCGT
GCTGCTGGTCTCCATCGGGGTCGTCATTTTCCTGGTCCTGGGGGTCATCCCCCGGTTCGCGACCTTTCTGAACGCCCGTA
ACGCCGCCCTGCCGGCATCGACCCAGGTACTGATGAATATTTCAGCCTGGGCCCAGGATTACGGCCGCATCCTCGCGATC
GCGCTGGGCGCCGTCTTCTTCTCCCTTTTTGTGGCGTACACGACCAGGAAAGGGAAGCAATTCCTGGACAGGGTGCTGCT
GACCCTGCCCCTGGTGGGTAAAGCGATCCTGTTCGCCTCCATGGCCCAGGCCGGATGGTGTCTTGCCATGCTTCTGAGAA
GCGGCGTGACGGCCCTGGAGTCGATCCGCATCGCCGCCAGCGTCATGGGAAACAGCGCCGTCAGCGATTGTTTCAGCGCG
GCGGCGGACGGGTTGCTGCAGGGGCAGTCCCTGTCCAAGACCCTGGACCAGCCCCATATCCCGCTGATGATGCGGCACAT
GGCGGCGGTCGGCGAGAGCAGCGGCCAGCTGGATACGGTAATGCACGGCGCGGGAGAATATTACCAGAAAGAGTTGACCG
CCAGGGTGAAACTCATCTCCGTGGCGATCGAACCGATGCTGATTCTGATGGTCGGGGTTGTGGTCGGGTTTGTCTACTTC
GCTTTTTTCCAGGCAGTGATGTCTGTTTCGAAGGGTGGGATGTGA

Upstream 100 bases:

>100_bases
CGGAAATTACACAACCCTCTGGCAGGACGCCCGCGAGAAGGTTCTGCGGGGCGAGGTCTCCCTTGCCGAAGTGCTCCACG
TAAGGGCGGAGGCGAACTAG

Downstream 100 bases:

>100_bases
TGCGGCCGGTACATTCAAGATCAACATGTCTGGCAGCGCGCATGCTGCTGGTTTTCCTGTCCGTTGCGTCCGTCAGCTAT
GGGGCCGCTATCGCCCCCGT

Product: type II secretion system protein

Products: NA

Alternate protein names: Pectic enzymes secretion protein outF [H]

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MSLYSFIGLDGQGRELRETIEAETEKDVARLLRNRSIFVLKVWEGGGAKPGNGFREWLARLKPLLRPGQYAPVGAGDLVI
FFRQTAMMLRAGYTLVTALDASSEMTAKLRLRRTIKKMCDEIRKGGSFSGTLAGEKKIFTPMVSNLIASGEKSGNLDDIL
DRLADSIERSKDIKRQLVAAMFYPLFVLLVSIGVVIFLVLGVIPRFATFLNARNAALPASTQVLMNISAWAQDYGRILAI
ALGAVFFSLFVAYTTRKGKQFLDRVLLTLPLVGKAILFASMAQAGWCLAMLLRSGVTALESIRIAASVMGNSAVSDCFSA
AADGLLQGQSLSKTLDQPHIPLMMRHMAAVGESSGQLDTVMHGAGEYYQKELTARVKLISVAIEPMLILMVGVVVGFVYF
AFFQAVMSVSKGGM

Sequences:

>Translated_414_residues
MSLYSFIGLDGQGRELRETIEAETEKDVARLLRNRSIFVLKVWEGGGAKPGNGFREWLARLKPLLRPGQYAPVGAGDLVI
FFRQTAMMLRAGYTLVTALDASSEMTAKLRLRRTIKKMCDEIRKGGSFSGTLAGEKKIFTPMVSNLIASGEKSGNLDDIL
DRLADSIERSKDIKRQLVAAMFYPLFVLLVSIGVVIFLVLGVIPRFATFLNARNAALPASTQVLMNISAWAQDYGRILAI
ALGAVFFSLFVAYTTRKGKQFLDRVLLTLPLVGKAILFASMAQAGWCLAMLLRSGVTALESIRIAASVMGNSAVSDCFSA
AADGLLQGQSLSKTLDQPHIPLMMRHMAAVGESSGQLDTVMHGAGEYYQKELTARVKLISVAIEPMLILMVGVVVGFVYF
AFFQAVMSVSKGGM
>Mature_413_residues
SLYSFIGLDGQGRELRETIEAETEKDVARLLRNRSIFVLKVWEGGGAKPGNGFREWLARLKPLLRPGQYAPVGAGDLVIF
FRQTAMMLRAGYTLVTALDASSEMTAKLRLRRTIKKMCDEIRKGGSFSGTLAGEKKIFTPMVSNLIASGEKSGNLDDILD
RLADSIERSKDIKRQLVAAMFYPLFVLLVSIGVVIFLVLGVIPRFATFLNARNAALPASTQVLMNISAWAQDYGRILAIA
LGAVFFSLFVAYTTRKGKQFLDRVLLTLPLVGKAILFASMAQAGWCLAMLLRSGVTALESIRIAASVMGNSAVSDCFSAA
ADGLLQGQSLSKTLDQPHIPLMMRHMAAVGESSGQLDTVMHGAGEYYQKELTARVKLISVAIEPMLILMVGVVVGFVYFA
FFQAVMSVSKGGM

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of the multiple pectic enzymes [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=408, Percent_Identity=25.9803921568627, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1786295, Length=330, Percent_Identity=22.7272727272727, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR011850
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 45014; Mature: 44883

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: PS00874 T2SP_F

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLYSFIGLDGQGRELRETIEAETEKDVARLLRNRSIFVLKVWEGGGAKPGNGFREWLAR
CCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHH
LKPLLRPGQYAPVGAGDLVIFFRQTAMMLRAGYTLVTALDASSEMTAKLRLRRTIKKMCD
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHEEEECCCHHHHHHHHHHHHHHHHHH
EIRKGGSFSGTLAGEKKIFTPMVSNLIASGEKSGNLDDILDRLADSIERSKDIKRQLVAA
HHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MFYPLFVLLVSIGVVIFLVLGVIPRFATFLNARNAALPASTQVLMNISAWAQDYGRILAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ALGAVFFSLFVAYTTRKGKQFLDRVLLTLPLVGKAILFASMAQAGWCLAMLLRSGVTALE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
SIRIAASVMGNSAVSDCFSAAADGLLQGQSLSKTLDQPHIPLMMRHMAAVGESSGQLDTV
HHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHH
MHGAGEYYQKELTARVKLISVAIEPMLILMVGVVVGFVYFAFFQAVMSVSKGGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SLYSFIGLDGQGRELRETIEAETEKDVARLLRNRSIFVLKVWEGGGAKPGNGFREWLAR
CCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHH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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8326859 [H]