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The map label for this gene is pulE [H]

Identifier: 118579253

GI number: 118579253

Start: 860479

End: 862098

Strand: Direct

Name: pulE [H]

Synonym: Ppro_0815

Alternate gene names: 118579253

Gene position: 860479-862098 (Clockwise)

Preceding gene: 118579252

Following gene: 118579254

Centisome position: 21.47

GC content: 66.17

Gene sequence:

>1620_bases
ATGCCGGTAAACGAAACCATTCTGATCGAGGCCGCCATCAAGACCGGCCTGCTCAGGGAAGCCGATATCAAGACGTTGCG
GTTGCAGGCCAAACGGGAGCGGCTCCCCCTGCTGGAGGTGATCACCAGGGCGGGCAGGTTTCCCGAGGCCGCTCTCTACC
AGTCCCTGGCCGACGCGCGGGGGATTCCCTTTCTCCTGCCGGAGCAGCTGACGGCCGATGCGGAAGCGCTGGCGCTCCTG
CCCCAGGGGAGCCGGCAGCACCGGCTGCTGTTGCCGGTGCGGGACGCGGACGGCGGCAGGCTGCTGGCGCTGGCGGATCC
CGATGATCACCTGAGCCTGGAGCGTGTCGAACGCGCCACCGGTGAACGCTATCGTCCCGCTCTTGCGGAACCCGCGGCGC
TCCGCGGCGCGCTCGAGCGGTGCGGCCAGGGGGGGGCGGCATGGCCCGCGGCGGCGGAAAAGAATGATCCGGTCCAGTTT
CTGGACGACGTCATGAAGGAGGTGTACCTGCGCCGCGCCTCCGACGTGCACTTCGAGCCCCAGGAGGGGAGGATGAGAAT
CCGCCTGCGGGTGGACGGCCGCATGCAGGAGTTCGGCCGCATGCTGCCGGCCTCGGATGAAGATGCCCTGCTGAACCGGA
TCAAGGTGCTGGCGGGGCTGGATATCGCCGAGCAGCGCATGGCCCAGGACGGCGCCATGAAATACGCCATCATGAACTGG
GACATCCCGGAGGTGGATATCCGCGTCGCCACCATTCCAACCCGCTGGGGCGAGCGGGCGACCATGCGTATCCTCGGTCA
GGATACCGGCCGGTTGACGCTGGAGCAGCTGGGCATGCCGAAAACGATGCTCGGGGCGTTCCGCCGTGCCATTGCCCGGC
CCCATGGCGTGATTCTGGTGACCGGCCCCACCGGCAGCGGCAAGTCGACGACGCTGTACAGCGCCATTCGGGAGCTGGAC
GTGAACGCCATCAATGTGCTGACGGTTGAAGACCCGGTGGAACTGCCCATCGCCGGGGTCAGTCAGGTCCAGGTGAGCGT
CAAGGTCGGCTTTGCCGAGGCGCTGCGCTCGTTTCTGCGTCACGATCCCGATGTCATCCTGGTGGGGGAAATGCGCGACC
GGGAGACGGTTGATATCGGGCTGCGGGCGGCCATGACGGGGCACCTGGTGATGTCTACCCTCCATACCAACGATGCCGTC
GGAGCGGTTACCAGGCTCCTGGATATCGGCGCCGAGCGTTTTCTCATCGCTTCGACCCTGACCGGTGTGCTGGCCCAGCG
TCTGGTGCGGCGACTGTGCGTCAACTGCTGCCGGAAACGCCCGGCCCTGCCCGAGGAGCTGCGGATACTCAACCTGGAAG
GCGAAAACGTGGTGGAGATCTACGAGCCGGGGGGCTGCTCCTTTTGCCTCGGCAGCGGCTTCAGGGGGCGCATCGGGCTG
TTCGAGGCGTTCTGGATCGACCAGGATCTGAGTCTGGCGATCTCGGAAGGCGCAAGCGAGGTGAGGATACGGGAAATGGC
CGGAAATTACACAACCCTCTGGCAGGACGCCCGCGAGAAGGTTCTGCGGGGCGAGGTCTCCCTTGCCGAAGTGCTCCACG
TAAGGGCGGAGGCGAACTAG

Upstream 100 bases:

>100_bases
TCCAATAAAATTAGTCGGGAAACGGCCCTGACCTACTGCAACAACCGGGAAGGTTTCGACCGGCTGGTCAGTGTCGCCGT
GGAGCGGGAGTGAAGAGCAT

Downstream 100 bases:

>100_bases
ATGTCGCTCTATTCCTTTATCGGCCTTGATGGTCAAGGGCGGGAACTGCGGGAAACCATCGAGGCTGAAACCGAAAAGGA
TGTGGCGCGTCTTCTCCGAA

Product: type II secretion system protein E

Products: NA

Alternate protein names: Pullulanase secretion protein pulE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 539; Mature: 538

Protein sequence:

>539_residues
MPVNETILIEAAIKTGLLREADIKTLRLQAKRERLPLLEVITRAGRFPEAALYQSLADARGIPFLLPEQLTADAEALALL
PQGSRQHRLLLPVRDADGGRLLALADPDDHLSLERVERATGERYRPALAEPAALRGALERCGQGGAAWPAAAEKNDPVQF
LDDVMKEVYLRRASDVHFEPQEGRMRIRLRVDGRMQEFGRMLPASDEDALLNRIKVLAGLDIAEQRMAQDGAMKYAIMNW
DIPEVDIRVATIPTRWGERATMRILGQDTGRLTLEQLGMPKTMLGAFRRAIARPHGVILVTGPTGSGKSTTLYSAIRELD
VNAINVLTVEDPVELPIAGVSQVQVSVKVGFAEALRSFLRHDPDVILVGEMRDRETVDIGLRAAMTGHLVMSTLHTNDAV
GAVTRLLDIGAERFLIASTLTGVLAQRLVRRLCVNCCRKRPALPEELRILNLEGENVVEIYEPGGCSFCLGSGFRGRIGL
FEAFWIDQDLSLAISEGASEVRIREMAGNYTTLWQDAREKVLRGEVSLAEVLHVRAEAN

Sequences:

>Translated_539_residues
MPVNETILIEAAIKTGLLREADIKTLRLQAKRERLPLLEVITRAGRFPEAALYQSLADARGIPFLLPEQLTADAEALALL
PQGSRQHRLLLPVRDADGGRLLALADPDDHLSLERVERATGERYRPALAEPAALRGALERCGQGGAAWPAAAEKNDPVQF
LDDVMKEVYLRRASDVHFEPQEGRMRIRLRVDGRMQEFGRMLPASDEDALLNRIKVLAGLDIAEQRMAQDGAMKYAIMNW
DIPEVDIRVATIPTRWGERATMRILGQDTGRLTLEQLGMPKTMLGAFRRAIARPHGVILVTGPTGSGKSTTLYSAIRELD
VNAINVLTVEDPVELPIAGVSQVQVSVKVGFAEALRSFLRHDPDVILVGEMRDRETVDIGLRAAMTGHLVMSTLHTNDAV
GAVTRLLDIGAERFLIASTLTGVLAQRLVRRLCVNCCRKRPALPEELRILNLEGENVVEIYEPGGCSFCLGSGFRGRIGL
FEAFWIDQDLSLAISEGASEVRIREMAGNYTTLWQDAREKVLRGEVSLAEVLHVRAEAN
>Mature_538_residues
PVNETILIEAAIKTGLLREADIKTLRLQAKRERLPLLEVITRAGRFPEAALYQSLADARGIPFLLPEQLTADAEALALLP
QGSRQHRLLLPVRDADGGRLLALADPDDHLSLERVERATGERYRPALAEPAALRGALERCGQGGAAWPAAAEKNDPVQFL
DDVMKEVYLRRASDVHFEPQEGRMRIRLRVDGRMQEFGRMLPASDEDALLNRIKVLAGLDIAEQRMAQDGAMKYAIMNWD
IPEVDIRVATIPTRWGERATMRILGQDTGRLTLEQLGMPKTMLGAFRRAIARPHGVILVTGPTGSGKSTTLYSAIRELDV
NAINVLTVEDPVELPIAGVSQVQVSVKVGFAEALRSFLRHDPDVILVGEMRDRETVDIGLRAAMTGHLVMSTLHTNDAVG
AVTRLLDIGAERFLIASTLTGVLAQRLVRRLCVNCCRKRPALPEELRILNLEGENVVEIYEPGGCSFCLGSGFRGRIGLF
EAFWIDQDLSLAISEGASEVRIREMAGNYTTLWQDAREKVLRGEVSLAEVLHVRAEAN

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pullulanase [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=381, Percent_Identity=43.5695538057743, Blast_Score=308, Evalue=7e-85,
Organism=Escherichia coli, GI1786296, Length=438, Percent_Identity=37.8995433789954, Blast_Score=270, Evalue=3e-73,
Organism=Escherichia coli, GI87082188, Length=209, Percent_Identity=40.6698564593301, Blast_Score=115, Evalue=9e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 59468; Mature: 59336

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVNETILIEAAIKTGLLREADIKTLRLQAKRERLPLLEVITRAGRFPEAALYQSLADAR
CCCCCEEEEEEHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHC
GIPFLLPEQLTADAEALALLPQGSRQHRLLLPVRDADGGRLLALADPDDHLSLERVERAT
CCCEECCHHHCCCCHHEEECCCCCCCCEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHC
GERYRPALAEPAALRGALERCGQGGAAWPAAAEKNDPVQFLDDVMKEVYLRRASDVHFEP
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
QEGRMRIRLRVDGRMQEFGRMLPASDEDALLNRIKVLAGLDIAEQRMAQDGAMKYAIMNW
CCCCEEEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEEEEC
DIPEVDIRVATIPTRWGERATMRILGQDTGRLTLEQLGMPKTMLGAFRRAIARPHGVILV
CCCCEEEEEEEECCCCCCCEEEEEEECCCCCEEHHHCCCCHHHHHHHHHHHCCCCCEEEE
TGPTGSGKSTTLYSAIRELDVNAINVLTVEDPVELPIAGVSQVQVSVKVGFAEALRSFLR
ECCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCEEEEEEEECHHHHHHHHHH
HDPDVILVGEMRDRETVDIGLRAAMTGHLVMSTLHTNDAVGAVTRLLDIGAERFLIASTL
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHH
TGVLAQRLVRRLCVNCCRKRPALPEELRILNLEGENVVEIYEPGGCSFCLGSGFRGRIGL
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEECCCCCEEEECCCCCCCCCC
FEAFWIDQDLSLAISEGASEVRIREMAGNYTTLWQDAREKVLRGEVSLAEVLHVRAEAN
EEEEECCCCCHHEECCCCCCEEHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
PVNETILIEAAIKTGLLREADIKTLRLQAKRERLPLLEVITRAGRFPEAALYQSLADAR
CCCCEEEEEEHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHC
GIPFLLPEQLTADAEALALLPQGSRQHRLLLPVRDADGGRLLALADPDDHLSLERVERAT
CCCEECCHHHCCCCHHEEECCCCCCCCEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHC
GERYRPALAEPAALRGALERCGQGGAAWPAAAEKNDPVQFLDDVMKEVYLRRASDVHFEP
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
QEGRMRIRLRVDGRMQEFGRMLPASDEDALLNRIKVLAGLDIAEQRMAQDGAMKYAIMNW
CCCCEEEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEEEEC
DIPEVDIRVATIPTRWGERATMRILGQDTGRLTLEQLGMPKTMLGAFRRAIARPHGVILV
CCCCEEEEEEEECCCCCCCEEEEEEECCCCCEEHHHCCCCHHHHHHHHHHHCCCCCEEEE
TGPTGSGKSTTLYSAIRELDVNAINVLTVEDPVELPIAGVSQVQVSVKVGFAEALRSFLR
ECCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCEEEEEEEECHHHHHHHHHH
HDPDVILVGEMRDRETVDIGLRAAMTGHLVMSTLHTNDAVGAVTRLLDIGAERFLIASTL
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHH
TGVLAQRLVRRLCVNCCRKRPALPEELRILNLEGENVVEIYEPGGCSFCLGSGFRGRIGL
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEECCCCCEEEECCCCCCCCCC
FEAFWIDQDLSLAISEGASEVRIREMAGNYTTLWQDAREKVLRGEVSLAEVLHVRAEAN
EEEEECCCCCHHEECCCCCCEEHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1738317; 2677007 [H]