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The map label for this gene is pilD [H]

Identifier: 118579238

GI number: 118579238

Start: 846410

End: 847177

Strand: Reverse

Name: pilD [H]

Synonym: Ppro_0800

Alternate gene names: 118579238

Gene position: 847177-846410 (Counterclockwise)

Preceding gene: 118579239

Following gene: 118579237

Centisome position: 21.14

GC content: 60.29

Gene sequence:

>768_bases
ATGACGTCACAGTTCTATTATTCCATCATTGTCTTTATCTTCGGAGCCGTGACCGGTTCATTCCTCAATGTCTGCATATG
CCGCATGCCGAAGGACGAATCGGTCGTATCGCCCCCCTCCCATTGCCCAACATGCATGTACCGGATCCGCTGGTACGACA
ATATTCCCCTGGCCAGCTATCTGCTGCTCCGCGGCAGGTGCCGCGGCTGTGGCATCCGTATCCCCATCCGGTATCCGCTG
GTCGAGCTGGGTAACGGGCTGCTGGCACTGGCGCTCTTTCTCCGTTACAGCCTTTCCCCGACATTCGCTGCCCTGTTCCT
GTTCTGTTCGGCGCTGCTGGTGATCACCTGCATCGATCTGGAGTACCGGGTCATCCCGAACAAGATATCATTGCCGGGAA
TCGTTATCGGCTTTGTCCTTTCCTTTTTCCTCCCCTGGCCCGGGTGGCTGAATTCGCTGCTCGGCATTCTGGCGGGGGGC
GGCAGCCTGCTGCTGGTGGCCTGCGCCTACGAGTGGCTGACCGGCAGGGAGGGGATGGGAGGAGGCGACATCAAGCTGGT
GGCCATGATGGGGGCGTTCCTCGGTTGGGAAGCCGTCCCGTTCATCATCCTTGCCGGCTCCCTGGCCGGTTTGCTGGCAG
GGGGCCTGCCGATGCTGATCAGGAGGCGAGACTCCGGCCAGGCCATTCCCTTCGGCCCGTACCTTGCTTTCGGAGCGCTG
TTTCACCTCTTCCTCGGTCTCAGGATACTGAACCGGTATCTTGCGTAG

Upstream 100 bases:

>100_bases
GGATGAACTATCGGGAGAGATGTCCCGCATAACCGGGGGAAGGAGCATCCCGGGGGTTTTCTGACGGATTGTTCCGTTGG
AATCGGTGTAAAGGTTTTCC

Downstream 100 bases:

>100_bases
GGCGGCTTCCGTAAAGGGTAGAATTCCAGGGCAGACGTATCATTCCGGGACGCCTGAGGGACAAGTGCTTGCTGATGTGC
CATTACCGGAATTGGCGCGA

Product: prepilin peptidase

Products: NA

Alternate protein names: Protein pilD; Protein secretion protein XCPA; Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MTSQFYYSIIVFIFGAVTGSFLNVCICRMPKDESVVSPPSHCPTCMYRIRWYDNIPLASYLLLRGRCRGCGIRIPIRYPL
VELGNGLLALALFLRYSLSPTFAALFLFCSALLVITCIDLEYRVIPNKISLPGIVIGFVLSFFLPWPGWLNSLLGILAGG
GSLLLVACAYEWLTGREGMGGGDIKLVAMMGAFLGWEAVPFIILAGSLAGLLAGGLPMLIRRRDSGQAIPFGPYLAFGAL
FHLFLGLRILNRYLA

Sequences:

>Translated_255_residues
MTSQFYYSIIVFIFGAVTGSFLNVCICRMPKDESVVSPPSHCPTCMYRIRWYDNIPLASYLLLRGRCRGCGIRIPIRYPL
VELGNGLLALALFLRYSLSPTFAALFLFCSALLVITCIDLEYRVIPNKISLPGIVIGFVLSFFLPWPGWLNSLLGILAGG
GSLLLVACAYEWLTGREGMGGGDIKLVAMMGAFLGWEAVPFIILAGSLAGLLAGGLPMLIRRRDSGQAIPFGPYLAFGAL
FHLFLGLRILNRYLA
>Mature_254_residues
TSQFYYSIIVFIFGAVTGSFLNVCICRMPKDESVVSPPSHCPTCMYRIRWYDNIPLASYLLLRGRCRGCGIRIPIRYPLV
ELGNGLLALALFLRYSLSPTFAALFLFCSALLVITCIDLEYRVIPNKISLPGIVIGFVLSFFLPWPGWLNSLLGILAGGG
SLLLVACAYEWLTGREGMGGGDIKLVAMMGAFLGWEAVPFIILAGSLAGLLAGGLPMLIRRRDSGQAIPFGPYLAFGALF
HLFLGLRILNRYLA

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue. Processes the pilin precursor during membrane translocation. Required for the assembly of type IV pili and for secretion of most proteins [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=260, Percent_Identity=31.9230769230769, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1789732, Length=140, Percent_Identity=34.2857142857143, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 27929; Mature: 27798

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSQFYYSIIVFIFGAVTGSFLNVCICRMPKDESVVSPPSHCPTCMYRIRWYDNIPLASY
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHEEEECCCCCHHHH
LLLRGRCRGCGIRIPIRYPLVELGNGLLALALFLRYSLSPTFAALFLFCSALLVITCIDL
HHHHCCCCCCCEEEEECCCHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
EYRVIPNKISLPGIVIGFVLSFFLPWPGWLNSLLGILAGGGSLLLVACAYEWLTGREGMG
EEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCC
GGDIKLVAMMGAFLGWEAVPFIILAGSLAGLLAGGLPMLIRRRDSGQAIPFGPYLAFGAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCCCCCCHHHHHHHHH
FHLFLGLRILNRYLA
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSQFYYSIIVFIFGAVTGSFLNVCICRMPKDESVVSPPSHCPTCMYRIRWYDNIPLASY
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHEEEECCCCCHHHH
LLLRGRCRGCGIRIPIRYPLVELGNGLLALALFLRYSLSPTFAALFLFCSALLVITCIDL
HHHHCCCCCCCEEEEECCCHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
EYRVIPNKISLPGIVIGFVLSFFLPWPGWLNSLLGILAGGGSLLLVACAYEWLTGREGMG
EEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCC
GGDIKLVAMMGAFLGWEAVPFIILAGSLAGLLAGGLPMLIRRRDSGQAIPFGPYLAFGAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCCCCCCHHHHHHHHH
FHLFLGLRILNRYLA
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7905475 [H]