Click here to switch to the map view.

The map label for this gene is ispE

Identifier: 118579177

GI number: 118579177

Start: 777229

End: 778071

Strand: Direct

Name: ispE

Synonym: Ppro_0738

Alternate gene names: 118579177

Gene position: 777229-778071 (Clockwise)

Preceding gene: 118579168

Following gene: 118579178

Centisome position: 19.39

GC content: 62.4

Gene sequence:

>843_bases
GTGAAAAGCCTGACACTCAAAGCCCCAGCCAAGGTCAACTATCTGCTGGATGTCATTCGACGCCGCCCCGATGGGTACCA
TGACCTGCGCATGGTCATGCAGCGGGTCAACCTGTGTGACGAGATAGCCATCGCTCTGACCGACAGCCCGGAACTGTCCG
TGTGCTGCGGAAAAGACGGCGTGCCTGACGGTCCCGGCAATATCGCCTGGAAAGCGGCCCGCGCCATGCTGGATCTGGCC
GCCGGCGGACAGGGGGCGACCATCTCCATCGTCAAGAACATCCCGGTGGCCGCCGGCCTGGGTGGCGGCAGCAGTGATGC
AGCCACGGTGCTGATGGGAATGAACCAACTTCTGGAGCTGGGGCTCACGGATCAGGAACTGATGCGCATCGGCGTCAAGC
TGGGCGCTGATGTGCCGTTCTTTATTTTCAAAAAAACAGCCCTGGCCGAAGGGATCGGCGAGCAGTTGCGGGCCATGCCG
CCCATGCCCGCGCTCTGGGTGCTGCTGGTGAATCCGGGTGTGCATGTCTCGACAGCCTGGGTGTACAAAAATTTGCGGTT
GACAAGCCGTGGCGAGCTGGCTAAACTCCCCCAGTTTTTCAGCACGGTCGAGGACGTTTGCTCCATGCTTTCCAACGATC
TGGAGTCGGTGACCATTCCTGCTTTCCCGGTGATCGCCGACATCAAGCGCGCCATGCTGGCCAAGGGTGCCCTGGGTGCC
ATGATGTCCGGCAGCGGCCCCACGGTCTTCGGGCTCTTCCGCGACCGGGAGGGTGCGGAAGCGGCCCGTGCGGAACTGGT
CGAGGGGAGTGGATGGTTCGCGGCGGTGGCTGAGACTCTCTGA

Upstream 100 bases:

>100_bases
GCTGTTCCTCCGCCCGATCCTCTTTTGGCTTGATTTGTCCTCCCCTTTGCCGGTAGATTGGGGGCGCAAACGACTTTTTC
AAGAGGAGCGGACGGTTAAC

Downstream 100 bases:

>100_bases
ACCTTCTGAATTACTCCGTTTATTCTAAGGCGGATGACATAGCTTGTTGGGGCGTCGCCAAGCGGTAAGGCACCGGATTT
TGATTCCGGCATTCCCAGGT

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MKSLTLKAPAKVNYLLDVIRRRPDGYHDLRMVMQRVNLCDEIAIALTDSPELSVCCGKDGVPDGPGNIAWKAARAMLDLA
AGGQGATISIVKNIPVAAGLGGGSSDAATVLMGMNQLLELGLTDQELMRIGVKLGADVPFFIFKKTALAEGIGEQLRAMP
PMPALWVLLVNPGVHVSTAWVYKNLRLTSRGELAKLPQFFSTVEDVCSMLSNDLESVTIPAFPVIADIKRAMLAKGALGA
MMSGSGPTVFGLFRDREGAEAARAELVEGSGWFAAVAETL

Sequences:

>Translated_280_residues
MKSLTLKAPAKVNYLLDVIRRRPDGYHDLRMVMQRVNLCDEIAIALTDSPELSVCCGKDGVPDGPGNIAWKAARAMLDLA
AGGQGATISIVKNIPVAAGLGGGSSDAATVLMGMNQLLELGLTDQELMRIGVKLGADVPFFIFKKTALAEGIGEQLRAMP
PMPALWVLLVNPGVHVSTAWVYKNLRLTSRGELAKLPQFFSTVEDVCSMLSNDLESVTIPAFPVIADIKRAMLAKGALGA
MMSGSGPTVFGLFRDREGAEAARAELVEGSGWFAAVAETL
>Mature_280_residues
MKSLTLKAPAKVNYLLDVIRRRPDGYHDLRMVMQRVNLCDEIAIALTDSPELSVCCGKDGVPDGPGNIAWKAARAMLDLA
AGGQGATISIVKNIPVAAGLGGGSSDAATVLMGMNQLLELGLTDQELMRIGVKLGADVPFFIFKKTALAEGIGEQLRAMP
PMPALWVLLVNPGVHVSTAWVYKNLRLTSRGELAKLPQFFSTVEDVCSMLSNDLESVTIPAFPVIADIKRAMLAKGALGA
MMSGSGPTVFGLFRDREGAEAARAELVEGSGWFAAVAETL

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=272, Percent_Identity=33.8235294117647, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_PELPD (A1ALZ9)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_900427.1
- ProteinModelPortal:   A1ALZ9
- SMR:   A1ALZ9
- STRING:   A1ALZ9
- GeneID:   4572010
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_0738
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   RIYNGLR
- PhylomeDB:   A1ALZ9
- ProtClustDB:   PRK14614
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 29698; Mature: 29698

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 137-137

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLTLKAPAKVNYLLDVIRRRPDGYHDLRMVMQRVNLCDEIAIALTDSPELSVCCGKDG
CCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHEEEEEECCCCEEEEECCCC
VPDGPGNIAWKAARAMLDLAAGGQGATISIVKNIPVAAGLGGGSSDAATVLMGMNQLLEL
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCEEECCCCCCHHHHHHHHHHHHHHHC
GLTDQELMRIGVKLGADVPFFIFKKTALAEGIGEQLRAMPPMPALWVLLVNPGVHVSTAW
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEHE
VYKNLRLTSRGELAKLPQFFSTVEDVCSMLSNDLESVTIPAFPVIADIKRAMLAKGALGA
EEECEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHEECCCHHHHHHHHHHHHHHHHHHH
MMSGSGPTVFGLFRDREGAEAARAELVEGSGWFAAVAETL
HCCCCCCEEEEEEECCCCHHHHHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure
MKSLTLKAPAKVNYLLDVIRRRPDGYHDLRMVMQRVNLCDEIAIALTDSPELSVCCGKDG
CCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHEEEEEECCCCEEEEECCCC
VPDGPGNIAWKAARAMLDLAAGGQGATISIVKNIPVAAGLGGGSSDAATVLMGMNQLLEL
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCEEECCCCCCHHHHHHHHHHHHHHHC
GLTDQELMRIGVKLGADVPFFIFKKTALAEGIGEQLRAMPPMPALWVLLVNPGVHVSTAW
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEHE
VYKNLRLTSRGELAKLPQFFSTVEDVCSMLSNDLESVTIPAFPVIADIKRAMLAKGALGA
EEECEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHEECCCHHHHHHHHHHHHHHHHHHH
MMSGSGPTVFGLFRDREGAEAARAELVEGSGWFAAVAETL
HCCCCCCEEEEEEECCCCHHHHHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA