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The map label for this gene is 118579175

Identifier: 118579175

GI number: 118579175

Start: 775041

End: 775925

Strand: Reverse

Name: 118579175

Synonym: Ppro_0736

Alternate gene names: NA

Gene position: 775925-775041 (Counterclockwise)

Preceding gene: 118579176

Following gene: 118579174

Centisome position: 19.36

GC content: 66.67

Gene sequence:

>885_bases
ATGAAATCGCTCAGGGTGGGCCTGATCCAGCAGAGCTGTTCCGCCGACCGCGCCGCGAACCTGGAGAAGAGCTGCGACAT
GATCGCCCGAGCTGCCGCCTCCGGAGCGGAGCTTGTCGTGCTGCAGGAACTGCACACCGGCCCCTACTTCTGCCAGACCG
AGGATCCCTCCCTGTTCGATCTGGCCGAGCCGATCCCCGGCCCCACCTGCGAAACACTGGGCCCACTGGCCGCCAGGTTG
GGGGTGGTGATCGTGGCGTCGCTCTTCGAGCGGCGCGCCCCCGGCCTGTACCACAACAGCGCCGTCGTATTCGAGAGGGA
CGGCTCCATGGCGGGAATGTACCGCAAGATGCACATCCCCGACGATCCCGGTTTCTATGAAAAATTCTACTTCACGCCGG
GCGACCTGGGCTTCGCTCCCATCCAGACCTCGCTGGGCAAACTGGGCGTACTGGTCTGCTGGGATCAGTGGTACCCGGAA
GCCGCCCGCCTGATGGCACTGGCCGGCGCCGACCTGCTGATCTACCCCACCGCCATCGGTTGGGACCCGGCGGACAGCGC
CGAGGAGCAGGCGCGCCAGCGGGATGCCTGGGTCACGGTCCAGCGCGGGCATACGGTGGCCAACGGCCTGCCGCTCTTGG
CGGTCAACCGGGTTGGCTTGGAGGCATCCACGGAGAAACCGGAAAACGGTATCCTCTTCTGGGGGACCAGCTTCGCCGCC
GGCCCCCAGGGGGAGATCCTGACCCAGGCAAGCCAGGACAGGGAGGAGGTCGTGCTGGTGGAACTGGATCTGGGGCGCAG
CGAAACGGTGCGCCGCATCTGGCCCTTCCTGCGCGACCGGCGCATCGACGCCTATGGGGAGATCCTGAAGCGCTACCGTG
ACTGA

Upstream 100 bases:

>100_bases
TTCCCGGGCGCGAGATCATCGCCATCGACTGCCTGCCGCTGCTGAAGCAGCACGGTTCGCTGCACTGCGTCACCATGCAA
CTCCCCCAGGGGGTGCTGGC

Downstream 100 bases:

>100_bases
CAGCGGGGGCGGCATCGTCGACACAGCCCGGTGGTTGACAGATAAAGTTTCTTTATGATATTTTTTTCAGTTCAAAATTT
TCGTGACAGCATCACCAGCC

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MKSLRVGLIQQSCSADRAANLEKSCDMIARAAASGAELVVLQELHTGPYFCQTEDPSLFDLAEPIPGPTCETLGPLAARL
GVVIVASLFERRAPGLYHNSAVVFERDGSMAGMYRKMHIPDDPGFYEKFYFTPGDLGFAPIQTSLGKLGVLVCWDQWYPE
AARLMALAGADLLIYPTAIGWDPADSAEEQARQRDAWVTVQRGHTVANGLPLLAVNRVGLEASTEKPENGILFWGTSFAA
GPQGEILTQASQDREEVVLVELDLGRSETVRRIWPFLRDRRIDAYGEILKRYRD

Sequences:

>Translated_294_residues
MKSLRVGLIQQSCSADRAANLEKSCDMIARAAASGAELVVLQELHTGPYFCQTEDPSLFDLAEPIPGPTCETLGPLAARL
GVVIVASLFERRAPGLYHNSAVVFERDGSMAGMYRKMHIPDDPGFYEKFYFTPGDLGFAPIQTSLGKLGVLVCWDQWYPE
AARLMALAGADLLIYPTAIGWDPADSAEEQARQRDAWVTVQRGHTVANGLPLLAVNRVGLEASTEKPENGILFWGTSFAA
GPQGEILTQASQDREEVVLVELDLGRSETVRRIWPFLRDRRIDAYGEILKRYRD
>Mature_294_residues
MKSLRVGLIQQSCSADRAANLEKSCDMIARAAASGAELVVLQELHTGPYFCQTEDPSLFDLAEPIPGPTCETLGPLAARL
GVVIVASLFERRAPGLYHNSAVVFERDGSMAGMYRKMHIPDDPGFYEKFYFTPGDLGFAPIQTSLGKLGVLVCWDQWYPE
AARLMALAGADLLIYPTAIGWDPADSAEEQARQRDAWVTVQRGHTVANGLPLLAVNRVGLEASTEKPENGILFWGTSFAA
GPQGEILTQASQDREEVVLVELDLGRSETVRRIWPFLRDRRIDAYGEILKRYRD

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=296, Percent_Identity=27.3648648648649, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI7706509, Length=308, Percent_Identity=27.5974025974026, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI297632348, Length=295, Percent_Identity=25.4237288135593, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI297632350, Length=295, Percent_Identity=25.4237288135593, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI5031947, Length=295, Percent_Identity=25.4237288135593, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI297632346, Length=198, Percent_Identity=28.2828282828283, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17533173, Length=305, Percent_Identity=29.8360655737705, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17556280, Length=288, Percent_Identity=24.3055555555556, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17557111, Length=277, Percent_Identity=27.4368231046931, Blast_Score=68, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6323383, Length=297, Percent_Identity=27.2727272727273, Blast_Score=108, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21358471, Length=313, Percent_Identity=30.6709265175719, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI17933642, Length=290, Percent_Identity=23.7931034482759, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21355835, Length=291, Percent_Identity=26.1168384879725, Blast_Score=85, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 32415; Mature: 32415

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLRVGLIQQSCSADRAANLEKSCDMIARAAASGAELVVLQELHTGPYFCQTEDPSLFD
CCCCHHHHHHHHCCCHHHCCHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEECCCCCHHH
LAEPIPGPTCETLGPLAARLGVVIVASLFERRAPGLYHNSAVVFERDGSMAGMYRKMHIP
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHEEEECCC
DDPGFYEKFYFTPGDLGFAPIQTSLGKLGVLVCWDQWYPEAARLMALAGADLLIYPTAIG
CCCCCCCEEEECCCCCCCHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEEECC
WDPADSAEEQARQRDAWVTVQRGHTVANGLPLLAVNRVGLEASTEKPENGILFWGTSFAA
CCCCCHHHHHHHHHCCEEEEECCCCHHCCCCEEEEEECCCCCCCCCCCCCEEEEECCCCC
GPQGEILTQASQDREEVVLVELDLGRSETVRRIWPFLRDRRIDAYGEILKRYRD
CCCCCCHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKSLRVGLIQQSCSADRAANLEKSCDMIARAAASGAELVVLQELHTGPYFCQTEDPSLFD
CCCCHHHHHHHHCCCHHHCCHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEECCCCCHHH
LAEPIPGPTCETLGPLAARLGVVIVASLFERRAPGLYHNSAVVFERDGSMAGMYRKMHIP
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHEEEECCC
DDPGFYEKFYFTPGDLGFAPIQTSLGKLGVLVCWDQWYPEAARLMALAGADLLIYPTAIG
CCCCCCCEEEECCCCCCCHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEEECC
WDPADSAEEQARQRDAWVTVQRGHTVANGLPLLAVNRVGLEASTEKPENGILFWGTSFAA
CCCCCHHHHHHHHHCCEEEEECCCCHHCCCCEEEEEECCCCCCCCCCCCCEEEEECCCCC
GPQGEILTQASQDREEVVLVELDLGRSETVRRIWPFLRDRRIDAYGEILKRYRD
CCCCCCHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]