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The map label for this gene is tuf1 [H]

Identifier: 118579104

GI number: 118579104

Start: 704085

End: 705275

Strand: Direct

Name: tuf1 [H]

Synonym: Ppro_0665

Alternate gene names: 118579104

Gene position: 704085-705275 (Clockwise)

Preceding gene: 118579103

Following gene: 118579105

Centisome position: 17.57

GC content: 56.84

Gene sequence:

>1191_bases
ATGGCGAAGGCTAAATTTGAGCGCACCAAACCGCATGTAAACATTGGCACGATTGGTCACGTCGACCACGGCAAGACCAC
GTTGACTGCCGCCATCACAAAGGTGCTGGCAGGCAAGGGTCAGGCAGAGTTCAAGGCTTTCGACCAGATTGACAACGCAC
CTGAAGAGCGTGAGCGCGGTATAACCATTGCCACCGCACACGTGGAATACGAGACTGACAAGCGTCACTATGCCCACGTT
GACTGTCCGGGTCATGCCGACTACGTCAAGAATATGATCACCGGCGCCGCTCAGATGGACGGAGCCATTCTGGTTGTTTC
CGCAGCTGACGGCCCTATGCCCCAGACCCGCGAGCACATCCTGCTCGCCCGTCAGGTTGGCGTACCCTATATTGTCGTGT
ACCTGAACAAGGCCGACATGGTCGATGACGAGGAGCTTCTTGAGTTGGTCGAGCTCGAAATCCGCGAGCTACTCTCCTCC
TATGACTTTCCCGGTGACGATATTCCTATCGTTAAAGGTTCCGCCCTCAAGGCGCTAGAAGGCGAAAAGAGTGAGTTGGG
CGAGGATTCCATCATCAAGCTGATGGACGCTGTTGATAGCTACATCCCCGATCCGGAGCGTGCGGTGGACAAGCCCTTCC
TGATGCCGGTAGAGGATGTGTTCTCCATCTCAGGTCGTGGTACCGTTGCCACCGGTCGTGTTGAGCGCGGCATAGTCAAG
GTTGGTGAGGAGGTCGAGATCGTTGGCATCAAGGCCACCGCCAAGACAACCGTCACCGGTGTGGAGATGTTCCGCAAGCT
TCTCGACGAGGGTCGTGCCGGAGACAATATCGGCGCACTGCTGCGCGGTATCAAGCGCGAGGAAATCGAGCGCGGTCAAG
TTCTTGCCAAGCCTGGTTCAATCACTCCGCACACCAAGTTCAAGGCTGAGGCCTATATCCTCAACAAGGAAGAGGGTGGA
CGTCACACCCCATTCTTCAACGGATACCGTCCCCAGTTCTATTTCCGGACAACGGACGTTACCGGTATTGTCGACCTGCC
GGCAGGCACTGAAATGGTCATGCCTGGCGACAACGTGGCTGTAACCGTCAACCTGATCACTCCGATCGCCATGGATGAAG
GTCTGCGCTTCGCCATCCGTGAAGGCGGCCGCACTGTTGGCGCCGGCGTCGTCAGCTCGATCATCGAATAG

Upstream 100 bases:

>100_bases
GGTTCGAGTCCGGTTGTGGGCTCCATTTCAGTAGAACAGCCAATAAACTGGTCATACGCACATCAAAGCAATAAACAGAC
TTCAAGGAGGGCCTGCTATC

Downstream 100 bases:

>100_bases
TCTATACTGCCGCGCGGAGCGTCTCAGGGCGCTCCGCGCGGATCGTTTTAAAGGATGCGTATATGAGAGATATAATTACC
CTCGGCTGCACCGAATGCAA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLAGKGQAEFKAFDQIDNAPEERERGITIATAHVEYETDKRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVYLNKADMVDDEELLELVELEIRELLSS
YDFPGDDIPIVKGSALKALEGEKSELGEDSIIKLMDAVDSYIPDPERAVDKPFLMPVEDVFSISGRGTVATGRVERGIVK
VGEEVEIVGIKATAKTTVTGVEMFRKLLDEGRAGDNIGALLRGIKREEIERGQVLAKPGSITPHTKFKAEAYILNKEEGG
RHTPFFNGYRPQFYFRTTDVTGIVDLPAGTEMVMPGDNVAVTVNLITPIAMDEGLRFAIREGGRTVGAGVVSSIIE

Sequences:

>Translated_396_residues
MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLAGKGQAEFKAFDQIDNAPEERERGITIATAHVEYETDKRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVYLNKADMVDDEELLELVELEIRELLSS
YDFPGDDIPIVKGSALKALEGEKSELGEDSIIKLMDAVDSYIPDPERAVDKPFLMPVEDVFSISGRGTVATGRVERGIVK
VGEEVEIVGIKATAKTTVTGVEMFRKLLDEGRAGDNIGALLRGIKREEIERGQVLAKPGSITPHTKFKAEAYILNKEEGG
RHTPFFNGYRPQFYFRTTDVTGIVDLPAGTEMVMPGDNVAVTVNLITPIAMDEGLRFAIREGGRTVGAGVVSSIIE
>Mature_395_residues
AKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLAGKGQAEFKAFDQIDNAPEERERGITIATAHVEYETDKRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVYLNKADMVDDEELLELVELEIRELLSSY
DFPGDDIPIVKGSALKALEGEKSELGEDSIIKLMDAVDSYIPDPERAVDKPFLMPVEDVFSISGRGTVATGRVERGIVKV
GEEVEIVGIKATAKTTVTGVEMFRKLLDEGRAGDNIGALLRGIKREEIERGQVLAKPGSITPHTKFKAEAYILNKEEGGR
HTPFFNGYRPQFYFRTTDVTGIVDLPAGTEMVMPGDNVAVTVNLITPIAMDEGLRFAIREGGRTVGAGVVSSIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=59.3908629441624, Blast_Score=498, Evalue=1e-141,
Organism=Homo sapiens, GI4503475, Length=448, Percent_Identity=29.9107142857143, Blast_Score=178, Evalue=8e-45,
Organism=Homo sapiens, GI223555963, Length=445, Percent_Identity=30.561797752809, Blast_Score=176, Evalue=5e-44,
Organism=Homo sapiens, GI5729864, Length=445, Percent_Identity=30.561797752809, Blast_Score=175, Evalue=5e-44,
Organism=Homo sapiens, GI4503471, Length=448, Percent_Identity=29.9107142857143, Blast_Score=174, Evalue=9e-44,
Organism=Homo sapiens, GI194018520, Length=433, Percent_Identity=28.8683602771363, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI194018522, Length=433, Percent_Identity=28.8683602771363, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI194097354, Length=433, Percent_Identity=28.8683602771363, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI46094014, Length=434, Percent_Identity=28.3410138248848, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI54607086, Length=347, Percent_Identity=28.5302593659942, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI4503507, Length=261, Percent_Identity=28.735632183908, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI82546879, Length=439, Percent_Identity=24.6013667425968, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI157426893, Length=276, Percent_Identity=27.1739130434783, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI45593140, Length=428, Percent_Identity=22.196261682243, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=79.4936708860759, Blast_Score=624, Evalue=1e-180,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=79.4936708860759, Blast_Score=624, Evalue=1e-180,
Organism=Escherichia coli, GI2367247, Length=378, Percent_Identity=29.8941798941799, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI48994988, Length=252, Percent_Identity=33.3333333333333, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1789108, Length=271, Percent_Identity=32.8413284132841, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788922, Length=145, Percent_Identity=34.4827586206897, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=51.6290726817043, Blast_Score=408, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=45.4780361757106, Blast_Score=353, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI17552884, Length=448, Percent_Identity=31.6964285714286, Blast_Score=177, Evalue=8e-45,
Organism=Caenorhabditis elegans, GI17569207, Length=448, Percent_Identity=31.6964285714286, Blast_Score=177, Evalue=8e-45,
Organism=Caenorhabditis elegans, GI32566303, Length=429, Percent_Identity=30.0699300699301, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI32566629, Length=352, Percent_Identity=30.1136363636364, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI115532067, Length=436, Percent_Identity=27.2935779816514, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI115532065, Length=436, Percent_Identity=27.2935779816514, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17506081, Length=299, Percent_Identity=28.7625418060201, Blast_Score=114, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=145, Percent_Identity=40.6896551724138, Blast_Score=98, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17509919, Length=282, Percent_Identity=26.241134751773, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17557151, Length=236, Percent_Identity=27.5423728813559, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=398, Percent_Identity=67.5879396984925, Blast_Score=549, Evalue=1e-157,
Organism=Saccharomyces cerevisiae, GI6325337, Length=441, Percent_Identity=30.3854875283447, Blast_Score=172, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6319594, Length=441, Percent_Identity=30.3854875283447, Blast_Score=172, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6322937, Length=455, Percent_Identity=24.1758241758242, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320377, Length=340, Percent_Identity=29.7058823529412, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=286, Percent_Identity=27.972027972028, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=243, Percent_Identity=30.4526748971193, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=172, Percent_Identity=32.5581395348837, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=59.5419847328244, Blast_Score=496, Evalue=1e-141,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=59.5419847328244, Blast_Score=496, Evalue=1e-141,
Organism=Drosophila melanogaster, GI19921738, Length=385, Percent_Identity=50.3896103896104, Blast_Score=415, Evalue=1e-116,
Organism=Drosophila melanogaster, GI45553807, Length=448, Percent_Identity=29.9107142857143, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI45553816, Length=448, Percent_Identity=29.9107142857143, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24651721, Length=448, Percent_Identity=29.9107142857143, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI17864154, Length=448, Percent_Identity=29.9107142857143, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24652838, Length=446, Percent_Identity=31.8385650224215, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI17137572, Length=446, Percent_Identity=31.8385650224215, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI19922690, Length=305, Percent_Identity=31.8032786885246, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI45550900, Length=436, Percent_Identity=27.5229357798165, Blast_Score=131, Evalue=9e-31,
Organism=Drosophila melanogaster, GI17137380, Length=340, Percent_Identity=29.7058823529412, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24647046, Length=282, Percent_Identity=28.7234042553192, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24647048, Length=282, Percent_Identity=28.7234042553192, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28572034, Length=165, Percent_Identity=35.1515151515151, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43080; Mature: 42949

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLAGKGQAEFKAFDQIDNAPEERERG
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHCC
ITIATAHVEYETDKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPYIVVYLNKADMVDDEELLELVELEIRELLSSYDFPGDDIPIVKGSALKALE
HHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHCC
GEKSELGEDSIIKLMDAVDSYIPDPERAVDKPFLMPVEDVFSISGRGTVATGRVERGIVK
CCHHHCCHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHCCCCCCEEEECHHHHHHHH
VGEEVEIVGIKATAKTTVTGVEMFRKLLDEGRAGDNIGALLRGIKREEIERGQVLAKPGS
CCCEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEECCCC
ITPHTKFKAEAYILNKEEGGRHTPFFNGYRPQFYFRTTDVTGIVDLPAGTEMVMPGDNVA
CCCCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCEEEECCCCEE
VTVNLITPIAMDEGLRFAIREGGRTVGAGVVSSIIE
EEEEEECCEECCCCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLAGKGQAEFKAFDQIDNAPEERERG
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHCC
ITIATAHVEYETDKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPYIVVYLNKADMVDDEELLELVELEIRELLSSYDFPGDDIPIVKGSALKALE
HHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHCC
GEKSELGEDSIIKLMDAVDSYIPDPERAVDKPFLMPVEDVFSISGRGTVATGRVERGIVK
CCHHHCCHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHCCCCCCEEEECHHHHHHHH
VGEEVEIVGIKATAKTTVTGVEMFRKLLDEGRAGDNIGALLRGIKREEIERGQVLAKPGS
CCCEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEECCCC
ITPHTKFKAEAYILNKEEGGRHTPFFNGYRPQFYFRTTDVTGIVDLPAGTEMVMPGDNVA
CCCCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCEEEECCCCEE
VTVNLITPIAMDEGLRFAIREGGRTVGAGVVSSIIE
EEEEEECCEECCCCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA