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The map label for this gene is nth [H]

Identifier: 118579102

GI number: 118579102

Start: 701729

End: 702385

Strand: Direct

Name: nth [H]

Synonym: Ppro_0663

Alternate gene names: 118579102

Gene position: 701729-702385 (Clockwise)

Preceding gene: 118579100

Following gene: 118579103

Centisome position: 17.51

GC content: 58.14

Gene sequence:

>657_bases
ATGAAAGTCGATGAAATTCACCGAGTGATGGCGCTCCTGCGCGAAGAATACGCCTCCTGGCGGACGCCGGCGGTCACGAT
TGTCGCCGAATGCGAACGCAGCCCGTTCAAGGTCCTGGTCTCCTGCATCATCTCCCTGCGCACCAAGGATGAAGTAACCG
CTGCTGCGTCGGCGCGCATGTTCGCCCGTGCCGACAGTGCGGAACGCATGCTGAAACTGGCAGAGGATGAAATCGCCGCT
CTGATCTATCCTGCAGGTTTCTACCGCACCAAGGCCGGTCAGATTCATGGAATCGCGCACCGCCTAGTGACGGAGTTCGG
GGGGAACGTGCCCGATGAGATGGAGGATTTGCTCAGGTTCAGGGGCGTCGGTCGCAAAACCGCCAATCTGGTGCTAACCC
TCGGGTTTGGAAAACCCGGCATCTGCGTCGATACACACGTGCATCGTATCTGCAACCGCCTCGGCTATGTCTCGACGCGC
TCCCCGGAGCAAACTGAAATGGCCCTGCGTGCACAACTGCCCGGGGAGTACTGGATTGCAATCAATGACCTGCTGGTGGC
ATTTGGACAGAACCACTGTCATCCGATTTCGCCTCGCTGCACAACCTGTCGCATAGCAGAATTCTGTTCTCGCGTCGGAG
TGAAAACATCCCGCTAA

Upstream 100 bases:

>100_bases
CCTGGCGTAGATGCAATCATGGAGCGCATACTAGCCGCACGCCGTGCAAAAGTAAAACCATCTCTTGCGCTGCCATCCTG
TGTTGGGCTAGCATGCCCAC

Downstream 100 bases:

>100_bases
ACACCTGCTCAGCCCGGTGATTGCCGGTTGAAGTTGTGTCGCCTCTGTATCTGGTAACTATTCGGTCGGCGAATAACGCT
CAGGTCGGTGAGATAAATAT

Product: HhH-GPD family protein

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MKVDEIHRVMALLREEYASWRTPAVTIVAECERSPFKVLVSCIISLRTKDEVTAAASARMFARADSAERMLKLAEDEIAA
LIYPAGFYRTKAGQIHGIAHRLVTEFGGNVPDEMEDLLRFRGVGRKTANLVLTLGFGKPGICVDTHVHRICNRLGYVSTR
SPEQTEMALRAQLPGEYWIAINDLLVAFGQNHCHPISPRCTTCRIAEFCSRVGVKTSR

Sequences:

>Translated_218_residues
MKVDEIHRVMALLREEYASWRTPAVTIVAECERSPFKVLVSCIISLRTKDEVTAAASARMFARADSAERMLKLAEDEIAA
LIYPAGFYRTKAGQIHGIAHRLVTEFGGNVPDEMEDLLRFRGVGRKTANLVLTLGFGKPGICVDTHVHRICNRLGYVSTR
SPEQTEMALRAQLPGEYWIAINDLLVAFGQNHCHPISPRCTTCRIAEFCSRVGVKTSR
>Mature_218_residues
MKVDEIHRVMALLREEYASWRTPAVTIVAECERSPFKVLVSCIISLRTKDEVTAAASARMFARADSAERMLKLAEDEIAA
LIYPAGFYRTKAGQIHGIAHRLVTEFGGNVPDEMEDLLRFRGVGRKTANLVLTLGFGKPGICVDTHVHRICNRLGYVSTR
SPEQTEMALRAQLPGEYWIAINDLLVAFGQNHCHPISPRCTTCRIAEFCSRVGVKTSR

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=42.3728813559322, Blast_Score=147, Evalue=5e-36,
Organism=Escherichia coli, GI1787920, Length=176, Percent_Identity=34.0909090909091, Blast_Score=117, Evalue=5e-28,
Organism=Escherichia coli, GI1789331, Length=189, Percent_Identity=25.3968253968254, Blast_Score=69, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17554540, Length=169, Percent_Identity=43.7869822485207, Blast_Score=153, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6324530, Length=188, Percent_Identity=33.5106382978723, Blast_Score=102, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6319304, Length=188, Percent_Identity=34.5744680851064, Blast_Score=97, Evalue=2e-21,
Organism=Drosophila melanogaster, GI45550361, Length=192, Percent_Identity=34.8958333333333, Blast_Score=134, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24319; Mature: 24319

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVDEIHRVMALLREEYASWRTPAVTIVAECERSPFKVLVSCIISLRTKDEVTAAASARM
CCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
FARADSAERMLKLAEDEIAALIYPAGFYRTKAGQIHGIAHRLVTEFGGNVPDEMEDLLRF
HHHCCHHHHHHHHHHHHHHHEEECCCHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
RGVGRKTANLVLTLGFGKPGICVDTHVHRICNRLGYVSTRSPEQTEMALRAQLPGEYWIA
HCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEH
INDLLVAFGQNHCHPISPRCTTCRIAEFCSRVGVKTSR
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKVDEIHRVMALLREEYASWRTPAVTIVAECERSPFKVLVSCIISLRTKDEVTAAASARM
CCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
FARADSAERMLKLAEDEIAALIYPAGFYRTKAGQIHGIAHRLVTEFGGNVPDEMEDLLRF
HHHCCHHHHHHHHHHHHHHHEEECCCHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
RGVGRKTANLVLTLGFGKPGICVDTHVHRICNRLGYVSTRSPEQTEMALRAQLPGEYWIA
HCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEH
INDLLVAFGQNHCHPISPRCTTCRIAEFCSRVGVKTSR
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]