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The map label for this gene is fusA [H]

Identifier: 118579095

GI number: 118579095

Start: 694151

End: 696235

Strand: Direct

Name: fusA [H]

Synonym: Ppro_0656

Alternate gene names: 118579095

Gene position: 694151-696235 (Clockwise)

Preceding gene: 118579094

Following gene: 118579096

Centisome position: 17.32

GC content: 60.77

Gene sequence:

>2085_bases
ATGGGACGGTTTGACACGAGCAAGATCCGCAATCTGGGTATCATCGCGCACGGTGGCGCCGGCAAAACTTCGCTGGCCGA
GGCAATCCTCTTCGATACAAAGATGATCGACCGACTGGGACGGGTAGACGACGGCACCGCTACCATGGATTTCGAGCCCG
AGGAGGTCAAACGCAAGATCTCCATTACCTCGGCCCTGGACCACTGCGAGTGGAAGGATCATTCCATTCACATCGTGGAT
ACCCCGGGCTATGGAAACTTCATCGCCGATACCCGCGCCTGCATGCGCGCCCTGGACTGCGCGGTGGTGATCCTCTCCGC
CATTTCCGGCGTCAAGGCCCAGACCGAGGAGGTCTGGAACTGGGCCAACGAGTTCGAAATTCCCCGCATCGCCTTCGTCA
ACAAGATGGACCGGGAGCGGGCCAGCTTCCTGCGCGCCATCGACGACATGGAAAAGTCGCTGGGTGCGCGCGGCGTAGCC
GTCCAAATGCCCATCGGCGCCGAGGCCGAGTTCGAGGGGATCATCGACCTGATCACCATGAAGGCCTGCTTCTACGCCAA
GGACGGCTCGGGCGACTACAGCCAGGGGGATATTCCCGAAGAGTGCCTGACCGAGGCCAACCGTCTGCGGGAACTCATGG
TGGAGACGGTTGCCGAGGCCTATGATGCCCTGACCGAGAAATATTTGGATACGGGCGAGTTGACCGAGGAAGAGATCCTG
GACGGCCTGCGGGTCGGCACCATGCGCAGTACCTTCACTGCTGTGCTGTGCGGCTCCGCCACCGCCAACATCGGTGTGGC
GCACCTGCTGGATGCCATCTGCGCCTATCTGCCGTCGCCCCTGGATCGCACCAAGGCGGTGGGCACCCATCCCAAGAGCG
GCGACATCGTTGAGCGCGCTCCCGACGAGGCGGAACCGTTCTCGGCGCTGGTATTCAAGACCACCTCGGATCCGTACACC
GGCAAGATCACCATTCTGCGGGTCTATTCCGGGGTCCTGAATTCCGACTCAATGGTTTTAAACTCCACCAAGGGGGTCGA
AGAGCGTATCGGGCAGCTCTACGAGCTGGAGGGGAAGAAACAGCATCCCATCAAGCAGGCGGTTGCCGGCGATATCGTGG
CGGTTGCCAAGCTGAAGGAGACCGTGACCGGCGACACGCTCTGCACCCCGGCCAACCCCATTGTCTTCGAGCCAGCCAGG
CAGCTGCTTCCGGTCATCTCCTACGCCATCGAGCCCAAGACCAAGGCAGATGAGGACAAGATCCACAACGCCCTCCACCG
CATGATCGAGGAAGAGCCGACCCTGGAGTCGCACCGCGATGCCAAGACCCGTGAGATGATTGTCTCCGGCATGGGGCAGG
TTCACCTGGAAGTGATCGTGGAGAAGATGAAACGCAAGTTCGGCGTCGACGTGGTGCTCAAGACCCCCAAGGTGCCTTAT
TTGGAAACCATCCGCGGTTCGGCCAAGGTACAGGGAAAGTACAAGAAACAGTCCGGCGGGCGCGGCCAGTACGGCGATTG
CTGGATCGAGATGTCGCCCGCGGAACGGGGCGATGGGTACATCTTCGAAGACAAGATCGTGGGGGGCGTAATTCCGCGCC
AGTACATCCCGGCCGTTGACAAGGGGATCAGCGACGCGGCCCAGGATGGATTCCTGGCCGGCTACCCGGTGGTGGACTTC
AGGGTAGCGCTCTACGACGGCTCCTTCCACACGGTGGACTCCTCGGAGATGGCCTTCAAGGTTGCCGGTTCCATGGCCTT
CAAGAAGGCCATGGAGGTCTGTAAGTCGGTGCTCTTGGAACCGATCATGAACATGAAGGTGACCGTGCCGGATGAGAACA
TGGGCGACGTCATCGGCGACTTGAACTCCCGTCGCGGCAAGGTGGTGGGGGTGGAGCCAAAGGCCAACTCCCAGATAATC
AGGGCGGTGGTGCCCATGTCCGAGGTCCTGGCTTACTCAAACGACCTGAAATCCATGACAAGCGACCGGGGCATGTTCAC
GGTGGAGTTCTCCCACTACGAAGAGCTACCCACGCATCTGGCGCAGAAGGTCATTGCCGAGGCGAATGCGGCCAAGAAGG
AATGA

Upstream 100 bases:

>100_bases
GACGTGCGTCGTGATGAATGAAGACGGTTGCGATGGCATGCCGTTCCAGTAGTGGAAACGTTGGTGTACCGATTTTATTT
CATTCCAAGGAGGAATCAGA

Downstream 100 bases:

>100_bases
GAGTAACACATTAAACTGCGGAATCCCGGACCAGTGCGGCCTACACGGTCGCCTGGTCCGGTATGCATGAATGCTTGGAG
GTTCCAATGGGTCACAGCCG

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G [H]

Number of amino acids: Translated: 694; Mature: 693

Protein sequence:

>694_residues
MGRFDTSKIRNLGIIAHGGAGKTSLAEAILFDTKMIDRLGRVDDGTATMDFEPEEVKRKISITSALDHCEWKDHSIHIVD
TPGYGNFIADTRACMRALDCAVVILSAISGVKAQTEEVWNWANEFEIPRIAFVNKMDRERASFLRAIDDMEKSLGARGVA
VQMPIGAEAEFEGIIDLITMKACFYAKDGSGDYSQGDIPEECLTEANRLRELMVETVAEAYDALTEKYLDTGELTEEEIL
DGLRVGTMRSTFTAVLCGSATANIGVAHLLDAICAYLPSPLDRTKAVGTHPKSGDIVERAPDEAEPFSALVFKTTSDPYT
GKITILRVYSGVLNSDSMVLNSTKGVEERIGQLYELEGKKQHPIKQAVAGDIVAVAKLKETVTGDTLCTPANPIVFEPAR
QLLPVISYAIEPKTKADEDKIHNALHRMIEEEPTLESHRDAKTREMIVSGMGQVHLEVIVEKMKRKFGVDVVLKTPKVPY
LETIRGSAKVQGKYKKQSGGRGQYGDCWIEMSPAERGDGYIFEDKIVGGVIPRQYIPAVDKGISDAAQDGFLAGYPVVDF
RVALYDGSFHTVDSSEMAFKVAGSMAFKKAMEVCKSVLLEPIMNMKVTVPDENMGDVIGDLNSRRGKVVGVEPKANSQII
RAVVPMSEVLAYSNDLKSMTSDRGMFTVEFSHYEELPTHLAQKVIAEANAAKKE

Sequences:

>Translated_694_residues
MGRFDTSKIRNLGIIAHGGAGKTSLAEAILFDTKMIDRLGRVDDGTATMDFEPEEVKRKISITSALDHCEWKDHSIHIVD
TPGYGNFIADTRACMRALDCAVVILSAISGVKAQTEEVWNWANEFEIPRIAFVNKMDRERASFLRAIDDMEKSLGARGVA
VQMPIGAEAEFEGIIDLITMKACFYAKDGSGDYSQGDIPEECLTEANRLRELMVETVAEAYDALTEKYLDTGELTEEEIL
DGLRVGTMRSTFTAVLCGSATANIGVAHLLDAICAYLPSPLDRTKAVGTHPKSGDIVERAPDEAEPFSALVFKTTSDPYT
GKITILRVYSGVLNSDSMVLNSTKGVEERIGQLYELEGKKQHPIKQAVAGDIVAVAKLKETVTGDTLCTPANPIVFEPAR
QLLPVISYAIEPKTKADEDKIHNALHRMIEEEPTLESHRDAKTREMIVSGMGQVHLEVIVEKMKRKFGVDVVLKTPKVPY
LETIRGSAKVQGKYKKQSGGRGQYGDCWIEMSPAERGDGYIFEDKIVGGVIPRQYIPAVDKGISDAAQDGFLAGYPVVDF
RVALYDGSFHTVDSSEMAFKVAGSMAFKKAMEVCKSVLLEPIMNMKVTVPDENMGDVIGDLNSRRGKVVGVEPKANSQII
RAVVPMSEVLAYSNDLKSMTSDRGMFTVEFSHYEELPTHLAQKVIAEANAAKKE
>Mature_693_residues
GRFDTSKIRNLGIIAHGGAGKTSLAEAILFDTKMIDRLGRVDDGTATMDFEPEEVKRKISITSALDHCEWKDHSIHIVDT
PGYGNFIADTRACMRALDCAVVILSAISGVKAQTEEVWNWANEFEIPRIAFVNKMDRERASFLRAIDDMEKSLGARGVAV
QMPIGAEAEFEGIIDLITMKACFYAKDGSGDYSQGDIPEECLTEANRLRELMVETVAEAYDALTEKYLDTGELTEEEILD
GLRVGTMRSTFTAVLCGSATANIGVAHLLDAICAYLPSPLDRTKAVGTHPKSGDIVERAPDEAEPFSALVFKTTSDPYTG
KITILRVYSGVLNSDSMVLNSTKGVEERIGQLYELEGKKQHPIKQAVAGDIVAVAKLKETVTGDTLCTPANPIVFEPARQ
LLPVISYAIEPKTKADEDKIHNALHRMIEEEPTLESHRDAKTREMIVSGMGQVHLEVIVEKMKRKFGVDVVLKTPKVPYL
ETIRGSAKVQGKYKKQSGGRGQYGDCWIEMSPAERGDGYIFEDKIVGGVIPRQYIPAVDKGISDAAQDGFLAGYPVVDFR
VALYDGSFHTVDSSEMAFKVAGSMAFKKAMEVCKSVLLEPIMNMKVTVPDENMGDVIGDLNSRRGKVVGVEPKANSQIIR
AVVPMSEVLAYSNDLKSMTSDRGMFTVEFSHYEELPTHLAQKVIAEANAAKKE

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=695, Percent_Identity=34.2446043165468, Blast_Score=401, Evalue=1e-112,
Organism=Homo sapiens, GI19923640, Length=711, Percent_Identity=30.379746835443, Blast_Score=322, Evalue=1e-87,
Organism=Homo sapiens, GI25306283, Length=393, Percent_Identity=34.8600508905852, Blast_Score=208, Evalue=1e-53,
Organism=Homo sapiens, GI25306287, Length=289, Percent_Identity=38.0622837370242, Blast_Score=177, Evalue=3e-44,
Organism=Homo sapiens, GI157426893, Length=153, Percent_Identity=29.4117647058824, Blast_Score=73, Evalue=9e-13,
Organism=Escherichia coli, GI1789738, Length=692, Percent_Identity=46.5317919075145, Blast_Score=608, Evalue=1e-175,
Organism=Escherichia coli, GI1790835, Length=483, Percent_Identity=24.8447204968944, Blast_Score=135, Evalue=8e-33,
Organism=Escherichia coli, GI48994988, Length=173, Percent_Identity=30.635838150289, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17533571, Length=694, Percent_Identity=32.9971181556196, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17556745, Length=720, Percent_Identity=26.1111111111111, Blast_Score=247, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6323098, Length=691, Percent_Identity=34.7322720694645, Blast_Score=423, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6322359, Length=801, Percent_Identity=27.7153558052434, Blast_Score=293, Evalue=7e-80,
Organism=Drosophila melanogaster, GI24582462, Length=677, Percent_Identity=35.8936484490399, Blast_Score=425, Evalue=1e-119,
Organism=Drosophila melanogaster, GI221458488, Length=732, Percent_Identity=27.8688524590164, Blast_Score=259, Evalue=6e-69,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 76100; Mature: 75969

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRFDTSKIRNLGIIAHGGAGKTSLAEAILFDTKMIDRLGRVDDGTATMDFEPEEVKRKI
CCCCCCHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHH
SITSALDHCEWKDHSIHIVDTPGYGNFIADTRACMRALDCAVVILSAISGVKAQTEEVWN
HHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
WANEFEIPRIAFVNKMDRERASFLRAIDDMEKSLGARGVAVQMPIGAEAEFEGIIDLITM
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHH
KACFYAKDGSGDYSQGDIPEECLTEANRLRELMVETVAEAYDALTEKYLDTGELTEEEIL
HHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
DGLRVGTMRSTFTAVLCGSATANIGVAHLLDAICAYLPSPLDRTKAVGTHPKSGDIVERA
HHHHHCHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCHHHCC
PDEAEPFSALVFKTTSDPYTGKITILRVYSGVLNSDSMVLNSTKGVEERIGQLYELEGKK
CCCCCCCEEEEEEECCCCCCCCEEEEEEHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCC
QHPIKQAVAGDIVAVAKLKETVTGDTLCTPANPIVFEPARQLLPVISYAIEPKTKADEDK
CCCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCEECCHHHHHHHHHHHCCCCCCCCHHH
IHNALHRMIEEEPTLESHRDAKTREMIVSGMGQVHLEVIVEKMKRKFGVDVVLKTPKVPY
HHHHHHHHHHCCCCCHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEEECCCCCH
LETIRGSAKVQGKYKKQSGGRGQYGDCWIEMSPAERGDGYIFEDKIVGGVIPRQYIPAVD
HHHHCCCCEECCCHHHCCCCCCCCCCEEEEECCCCCCCCEEEECCHHCCCCCHHHCCHHH
KGISDAAQDGFLAGYPVVDFRVALYDGSFHTVDSSEMAFKVAGSMAFKKAMEVCKSVLLE
CCCHHHHHCCCEECCCCEEEEEEEECCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
PIMNMKVTVPDENMGDVIGDLNSRRGKVVGVEPKANSQIIRAVVPMSEVLAYSNDLKSMT
HHHCCEEECCCCCHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SDRGMFTVEFSHYEELPTHLAQKVIAEANAAKKE
CCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GRFDTSKIRNLGIIAHGGAGKTSLAEAILFDTKMIDRLGRVDDGTATMDFEPEEVKRKI
CCCCCHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHH
SITSALDHCEWKDHSIHIVDTPGYGNFIADTRACMRALDCAVVILSAISGVKAQTEEVWN
HHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
WANEFEIPRIAFVNKMDRERASFLRAIDDMEKSLGARGVAVQMPIGAEAEFEGIIDLITM
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHH
KACFYAKDGSGDYSQGDIPEECLTEANRLRELMVETVAEAYDALTEKYLDTGELTEEEIL
HHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
DGLRVGTMRSTFTAVLCGSATANIGVAHLLDAICAYLPSPLDRTKAVGTHPKSGDIVERA
HHHHHCHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCHHHCC
PDEAEPFSALVFKTTSDPYTGKITILRVYSGVLNSDSMVLNSTKGVEERIGQLYELEGKK
CCCCCCCEEEEEEECCCCCCCCEEEEEEHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCC
QHPIKQAVAGDIVAVAKLKETVTGDTLCTPANPIVFEPARQLLPVISYAIEPKTKADEDK
CCCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCEECCHHHHHHHHHHHCCCCCCCCHHH
IHNALHRMIEEEPTLESHRDAKTREMIVSGMGQVHLEVIVEKMKRKFGVDVVLKTPKVPY
HHHHHHHHHHCCCCCHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEEECCCCCH
LETIRGSAKVQGKYKKQSGGRGQYGDCWIEMSPAERGDGYIFEDKIVGGVIPRQYIPAVD
HHHHCCCCEECCCHHHCCCCCCCCCCEEEEECCCCCCCCEEEECCHHCCCCCHHHCCHHH
KGISDAAQDGFLAGYPVVDFRVALYDGSFHTVDSSEMAFKVAGSMAFKKAMEVCKSVLLE
CCCHHHHHCCCEECCCCEEEEEEEECCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
PIMNMKVTVPDENMGDVIGDLNSRRGKVVGVEPKANSQIIRAVVPMSEVLAYSNDLKSMT
HHHCCEEECCCCCHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SDRGMFTVEFSHYEELPTHLAQKVIAEANAAKKE
CCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA