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The map label for this gene is hyfR [H]

Identifier: 118579021

GI number: 118579021

Start: 621482

End: 623035

Strand: Direct

Name: hyfR [H]

Synonym: Ppro_0582

Alternate gene names: 118579021

Gene position: 621482-623035 (Clockwise)

Preceding gene: 118579009

Following gene: 118579022

Centisome position: 15.51

GC content: 65.51

Gene sequence:

>1554_bases
ATGGTAAACAGGGATGAATTCTTCAGGGAGGTTACGGTCCGAATCTGCAGCAGCCTGGAGATCAAAACCGCCCTGCGCAG
CGCCTTCACCTATCTGCGGGAGTTCTTTCCCCTGGACATGCTCAGCCTCGGAATCTGTGATGCCAGCCTGTCGGCCATCC
GCAGGGTTGCCCATGTGACGGCCGACTCCACGGAGACCCATGATGAGATCATACCGGTTCCCGGGCAGCTCTGGGCATCG
CTGCAGCGCTGGGAGATGCGCTCGCCGGTGATGGTGGACTCGGACCAGAGTGGTCTGGTGCGGGCCCTGGCCCCCCACCT
GAAGCTGGACGAGAACTCCGGCATCCTGCTGCCGCTCTGGAGTGGGGAGGGGATGCTGGGCTGGCTGACACTGTACGCCT
GGGGGGCGGGACGCTTCGATGCCGGGCATGTGGCGTTGCTGGCAACGGTGGCCGACCCCTTCGTGATCGCCCTGGCCAAT
GCCTTGGCCCACGAAAAGCTGCTCAGGTACCGCGACAACCTGATCGACGATAACCGTTTCCTGAGCAAGGAACTCTTCAC
CCAGGGGGGGGACGAGATCGTCGGCGCCAACCTGGGGCTCAGGCACGTGATGGAGATGGTCCGCCAGGTGGCGCCGCTGA
ACAACACCGTGCTGCTGCTGGGGGAGACCGGCACCGGCAAGGAGCTGATCGCCAATGCCATCCACTCCGCATCACCCCGC
AAGGACGGTCCCCTGATCAAGGTCAACTGCGGCGCGATCCCGGAGCAACTCATCGACAGCGAGCTGTTCGGCCATGAGAA
GGGGGCCTTTACCGGAGCGGTAACGGAGCGCCGTGGACGCTTCGAGCGTGCCGACGGCGGCACCATCTTCCTGGACGAGA
TCGGCGAGCTGCCGCTCCAGGCCCAGGTGCGTCTCCTGCGGGTGCTCCAGAACCACGAGATAGATCGGGTAGGGGGCAGC
AGGCCGATTCCGGTGGACATCCGGGTGATCGCCGCCACCCACCGCAATCTGGAGAGCATGATTGCCGAGAACCGCTTTCG
CGAGGACCTCTGGTTTCGCCTGAACGTCTTTCCGCTGTTCATACCGCCCCTTCGCCAGAGGCGCGAGGACATACCCGGCC
TGACCCGTCATTTCATCAGCCAGAAATGTCGTGAGCTGGGCATGGCCGCCATGCCGGACATCGCGCCGGGCGCCCTGGTG
CGGCTGATGGAGTATGCCTGGCCGGGCAACGTGCGCGAGCTCGAGAACCTGGTGGAGCGGGAGCTGATTCTGCACCGGGG
AGGGACTCTGCAGTTCGACGCACTCATGCCGGCCGCCCGGCGGGGCAATGCTCCCGTTCAGCGGGAGGCGCCGGGCGTTG
AGGAGGGGCTGGAGCTTGACCAGGCCATCTCCCGGCACCTGCGCAGGGTCCTTGATCTGACCGGCGGACGGATACATGGC
CGTGGCGGGGCTGCGGAGCTGCTGGGTGTCAAGCCCAGCACCCTCAGGTGGCGTCTTGACCGGCTCGGCATTCCCCATGG
TCGCGGCGCGGGCGGGGAGCGGGTGAAATCCTGA

Upstream 100 bases:

>100_bases
TTTGTGGTTGCGATATATTGCACTTGTGGTAGTAAGTGCGGTATATTGCGATGTGGCCAGCTGTCTGCCACGCCAGTACC
GGTCCATAAGGGGGATGTGC

Downstream 100 bases:

>100_bases
CCGCCAGCGCCCGGGGGCGGGAGCCCCTTTGGATGCCACAAATTGTTCTTTGCAGCATGGGGCTGATTGTGGTAGTTTTT
TAAACTTTTGTGGCTCAGGC

Product: sigma-54 factor interaction domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MVNRDEFFREVTVRICSSLEIKTALRSAFTYLREFFPLDMLSLGICDASLSAIRRVAHVTADSTETHDEIIPVPGQLWAS
LQRWEMRSPVMVDSDQSGLVRALAPHLKLDENSGILLPLWSGEGMLGWLTLYAWGAGRFDAGHVALLATVADPFVIALAN
ALAHEKLLRYRDNLIDDNRFLSKELFTQGGDEIVGANLGLRHVMEMVRQVAPLNNTVLLLGETGTGKELIANAIHSASPR
KDGPLIKVNCGAIPEQLIDSELFGHEKGAFTGAVTERRGRFERADGGTIFLDEIGELPLQAQVRLLRVLQNHEIDRVGGS
RPIPVDIRVIAATHRNLESMIAENRFREDLWFRLNVFPLFIPPLRQRREDIPGLTRHFISQKCRELGMAAMPDIAPGALV
RLMEYAWPGNVRELENLVERELILHRGGTLQFDALMPAARRGNAPVQREAPGVEEGLELDQAISRHLRRVLDLTGGRIHG
RGGAAELLGVKPSTLRWRLDRLGIPHGRGAGGERVKS

Sequences:

>Translated_517_residues
MVNRDEFFREVTVRICSSLEIKTALRSAFTYLREFFPLDMLSLGICDASLSAIRRVAHVTADSTETHDEIIPVPGQLWAS
LQRWEMRSPVMVDSDQSGLVRALAPHLKLDENSGILLPLWSGEGMLGWLTLYAWGAGRFDAGHVALLATVADPFVIALAN
ALAHEKLLRYRDNLIDDNRFLSKELFTQGGDEIVGANLGLRHVMEMVRQVAPLNNTVLLLGETGTGKELIANAIHSASPR
KDGPLIKVNCGAIPEQLIDSELFGHEKGAFTGAVTERRGRFERADGGTIFLDEIGELPLQAQVRLLRVLQNHEIDRVGGS
RPIPVDIRVIAATHRNLESMIAENRFREDLWFRLNVFPLFIPPLRQRREDIPGLTRHFISQKCRELGMAAMPDIAPGALV
RLMEYAWPGNVRELENLVERELILHRGGTLQFDALMPAARRGNAPVQREAPGVEEGLELDQAISRHLRRVLDLTGGRIHG
RGGAAELLGVKPSTLRWRLDRLGIPHGRGAGGERVKS
>Mature_517_residues
MVNRDEFFREVTVRICSSLEIKTALRSAFTYLREFFPLDMLSLGICDASLSAIRRVAHVTADSTETHDEIIPVPGQLWAS
LQRWEMRSPVMVDSDQSGLVRALAPHLKLDENSGILLPLWSGEGMLGWLTLYAWGAGRFDAGHVALLATVADPFVIALAN
ALAHEKLLRYRDNLIDDNRFLSKELFTQGGDEIVGANLGLRHVMEMVRQVAPLNNTVLLLGETGTGKELIANAIHSASPR
KDGPLIKVNCGAIPEQLIDSELFGHEKGAFTGAVTERRGRFERADGGTIFLDEIGELPLQAQVRLLRVLQNHEIDRVGGS
RPIPVDIRVIAATHRNLESMIAENRFREDLWFRLNVFPLFIPPLRQRREDIPGLTRHFISQKCRELGMAAMPDIAPGALV
RLMEYAWPGNVRELENLVERELILHRGGTLQFDALMPAARRGNAPVQREAPGVEEGLELDQAISRHLRRVLDLTGGRIHG
RGGAAELLGVKPSTLRWRLDRLGIPHGRGAGGERVKS

Specific function: Required for induction of expression of the hydrogenase- 4 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=512, Percent_Identity=40.0390625, Blast_Score=331, Evalue=8e-92,
Organism=Escherichia coli, GI1789087, Length=395, Percent_Identity=44.5569620253165, Blast_Score=314, Evalue=1e-86,
Organism=Escherichia coli, GI1788550, Length=300, Percent_Identity=48.3333333333333, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1790437, Length=309, Percent_Identity=44.9838187702265, Blast_Score=254, Evalue=7e-69,
Organism=Escherichia coli, GI1790299, Length=284, Percent_Identity=48.2394366197183, Blast_Score=252, Evalue=4e-68,
Organism=Escherichia coli, GI1789233, Length=318, Percent_Identity=40.8805031446541, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI1788905, Length=276, Percent_Identity=44.9275362318841, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI87082152, Length=320, Percent_Identity=44.375, Blast_Score=231, Evalue=8e-62,
Organism=Escherichia coli, GI87081872, Length=253, Percent_Identity=46.2450592885375, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1786524, Length=244, Percent_Identity=48.3606557377049, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1787583, Length=319, Percent_Identity=38.2445141065831, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI87081858, Length=315, Percent_Identity=33.3333333333333, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1789828, Length=251, Percent_Identity=33.8645418326693, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 57442; Mature: 57442

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNRDEFFREVTVRICSSLEIKTALRSAFTYLREFFPLDMLSLGICDASLSAIRRVAHVT
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHC
ADSTETHDEIIPVPGQLWASLQRWEMRSPVMVDSDQSGLVRALAPHLKLDENSGILLPLW
CCCCCCCCCEECCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHCCCCEECCCCCEEEEEE
SGEGMLGWLTLYAWGAGRFDAGHVALLATVADPFVIALANALAHEKLLRYRDNLIDDNRF
CCCCHHHHHHHHHCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
LSKELFTQGGDEIVGANLGLRHVMEMVRQVAPLNNTVLLLGETGTGKELIANAIHSASPR
HHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCC
KDGPLIKVNCGAIPEQLIDSELFGHEKGAFTGAVTERRGRFERADGGTIFLDEIGELPLQ
CCCCEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCEEECCCCCCCCHH
AQVRLLRVLQNHEIDRVGGSRPIPVDIRVIAATHRNLESMIAENRFREDLWFRLNVFPLF
HHHHHHHHHHCCCHHHCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHEEEEEEEEEE
IPPLRQRREDIPGLTRHFISQKCRELGMAAMPDIAPGALVRLMEYAWPGNVRELENLVER
CCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHH
ELILHRGGTLQFDALMPAARRGNAPVQREAPGVEEGLELDQAISRHLRRVLDLTGGRIHG
HHHHHCCCCEEEHHHCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEC
RGGAAELLGVKPSTLRWRLDRLGIPHGRGAGGERVKS
CCCCHHEECCCCHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVNRDEFFREVTVRICSSLEIKTALRSAFTYLREFFPLDMLSLGICDASLSAIRRVAHVT
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHC
ADSTETHDEIIPVPGQLWASLQRWEMRSPVMVDSDQSGLVRALAPHLKLDENSGILLPLW
CCCCCCCCCEECCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHCCCCEECCCCCEEEEEE
SGEGMLGWLTLYAWGAGRFDAGHVALLATVADPFVIALANALAHEKLLRYRDNLIDDNRF
CCCCHHHHHHHHHCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
LSKELFTQGGDEIVGANLGLRHVMEMVRQVAPLNNTVLLLGETGTGKELIANAIHSASPR
HHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCC
KDGPLIKVNCGAIPEQLIDSELFGHEKGAFTGAVTERRGRFERADGGTIFLDEIGELPLQ
CCCCEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCEEECCCCCCCCHH
AQVRLLRVLQNHEIDRVGGSRPIPVDIRVIAATHRNLESMIAENRFREDLWFRLNVFPLF
HHHHHHHHHHCCCHHHCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHEEEEEEEEEE
IPPLRQRREDIPGLTRHFISQKCRELGMAAMPDIAPGALVRLMEYAWPGNVRELENLVER
CCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHH
ELILHRGGTLQFDALMPAARRGNAPVQREAPGVEEGLELDQAISRHLRRVLDLTGGRIHG
HHHHHCCCCEEEHHHCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEC
RGGAAELLGVKPSTLRWRLDRLGIPHGRGAGGERVKS
CCCCHHEECCCCHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]