Click here to switch to the map view.

The map label for this gene is pinE [C]

Identifier: 118578718

GI number: 118578718

Start: 272195

End: 272869

Strand: Reverse

Name: pinE [C]

Synonym: Ppro_0275

Alternate gene names: 118578718

Gene position: 272869-272195 (Counterclockwise)

Preceding gene: 118578720

Following gene: 118578717

Centisome position: 6.81

GC content: 39.85

Gene sequence:

>675_bases
ATGCCAGACGGCAGATTTGTCAGCTACATCAGGGTGTCTACAGCTAAACAAGGCGCAAGCGGTCTTGGACTCGAAGCCCA
AAAGAAAGTCATATATGATTATTTGAATTGTGGTAATTGGGAGCTGATCGCTGAATATATCGAGATTGAATCAGGCAAAA
TTGATAACCGCATAGAACTAAATGAAGCGTTGAAGCGTTGTCAGATGACGGGGGCAACCTTGATAATAGCTAAATTGGAT
AGGTTATCGAGAGATGTTCATTTTATTAGCTCTATTCAAAAATCAAATATTGAGTTTGTTGTTTGTGACATGCCATTTGC
AAACAAGTTTTCAATACACATTTTTGCTGCCTTAGCAGAAGAAGAACGCAGAATGATAAGCGAGCGCACAAAACTTGCTT
TGCAATCGGCAAAAGCAAGAGGCGTAAAACTTGGTAAGAATAATCTTACAATCGATGGAATCTGCAAAGGTAGCAAGAAT
AGCTTGCATGTAAGGATACAAAAAGCTGACAAATTTGCAAATCAAGTTGTACCAATAATACAAGAGCATATTGTTAATAA
ACTTAGCTTGAATCAAATTGCGCACGAGCTGAACAGCGCAAGCATACTTACAGCTCGGGGCAAGGTGGGCGCATGGACAG
CTAGAGCAGTAAGAAACGTATTAAACAGGATCTAG

Upstream 100 bases:

>100_bases
AAGATATTTTTGTAATGCTTTAATGGTGTTGCGCTAAATTAATTTTTGTGTAAACTACATAAACGCTCGTTTAAGCAAAT
TACATAAGAGAGGTCATTCT

Downstream 100 bases:

>100_bases
CCCCCCCTCAATTTATCTTGCAGAAGAAATTAAATTTAAACTGTTCGGGTGTTGAGCATATTTTATCCTGGTGCGAGTAA
TTAAAGAATCACAATATAAA

Product: resolvase domain-containing protein

Products: NA

Alternate protein names: Resolvase Domain Protein; Site-Specific Recombinase; Resolvase; Resolvase Domain; Resolvase-Like Serine Recombinase; Resolvase-Like Protein; Resolvase Family Protein; Recombinase; DNA Invertase; Resolvase N Terminal Domain Superfamily; Site-Specific Recombinase For Integration And Excision; Resolvase Family Site-Specific Recombinase; Resolvase N-Terminal; Phage Resolvase/Recombinase For Integration And Excision; Y4cG Protein; DNA-Invertase; Resolvase N Terminal Domain Family; Serine Recombinase

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MPDGRFVSYIRVSTAKQGASGLGLEAQKKVIYDYLNCGNWELIAEYIEIESGKIDNRIELNEALKRCQMTGATLIIAKLD
RLSRDVHFISSIQKSNIEFVVCDMPFANKFSIHIFAALAEEERRMISERTKLALQSAKARGVKLGKNNLTIDGICKGSKN
SLHVRIQKADKFANQVVPIIQEHIVNKLSLNQIAHELNSASILTARGKVGAWTARAVRNVLNRI

Sequences:

>Translated_224_residues
MPDGRFVSYIRVSTAKQGASGLGLEAQKKVIYDYLNCGNWELIAEYIEIESGKIDNRIELNEALKRCQMTGATLIIAKLD
RLSRDVHFISSIQKSNIEFVVCDMPFANKFSIHIFAALAEEERRMISERTKLALQSAKARGVKLGKNNLTIDGICKGSKN
SLHVRIQKADKFANQVVPIIQEHIVNKLSLNQIAHELNSASILTARGKVGAWTARAVRNVLNRI
>Mature_223_residues
PDGRFVSYIRVSTAKQGASGLGLEAQKKVIYDYLNCGNWELIAEYIEIESGKIDNRIELNEALKRCQMTGATLIIAKLDR
LSRDVHFISSIQKSNIEFVVCDMPFANKFSIHIFAALAEEERRMISERTKLALQSAKARGVKLGKNNLTIDGICKGSKNS
LHVRIQKADKFANQVVPIIQEHIVNKLSLNQIAHELNSASILTARGKVGAWTARAVRNVLNRI

Specific function: This Protein Catalyzes The Inversion Of An 1800-Bp E.Coli DNA Fragment, The P Region, Which Can Exist In Either Orientation. The Function Of The Inversion Is Not Yet Clear. [C]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 25004; Mature: 24873

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: PS00397 RECOMBINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDGRFVSYIRVSTAKQGASGLGLEAQKKVIYDYLNCGNWELIAEYIEIESGKIDNRIEL
CCCCCEEEEEEEECHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEH
NEALKRCQMTGATLIIAKLDRLSRDVHFISSIQKSNIEFVVCDMPFANKFSIHIFAALAE
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEHHHHHH
EERRMISERTKLALQSAKARGVKLGKNNLTIDGICKGSKNSLHVRIQKADKFANQVVPII
HHHHHHHHHHHHHHHHHHHCCEEECCCCCEEEEEECCCCCCEEEEEEHHHHHHHHHHHHH
QEHIVNKLSLNQIAHELNSASILTARGKVGAWTARAVRNVLNRI
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
PDGRFVSYIRVSTAKQGASGLGLEAQKKVIYDYLNCGNWELIAEYIEIESGKIDNRIEL
CCCCEEEEEEEECHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEH
NEALKRCQMTGATLIIAKLDRLSRDVHFISSIQKSNIEFVVCDMPFANKFSIHIFAALAE
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEHHHHHH
EERRMISERTKLALQSAKARGVKLGKNNLTIDGICKGSKNSLHVRIQKADKFANQVVPII
HHHHHHHHHHHHHHHHHHHCCEEECCCCCEEEEEECCCCCCEEEEEEHHHHHHHHHHHHH
QEHIVNKLSLNQIAHELNSASILTARGKVGAWTARAVRNVLNRI
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA