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The map label for this gene is cysD [H]

Identifier: 118578511

GI number: 118578511

Start: 70793

End: 71698

Strand: Direct

Name: cysD [H]

Synonym: Ppro_0063

Alternate gene names: 118578511

Gene position: 70793-71698 (Clockwise)

Preceding gene: 118578510

Following gene: 118578512

Centisome position: 1.77

GC content: 61.92

Gene sequence:

>906_bases
ATGAACGACCACACCGACTACCTGGACATTCTGGAACGGGAATCTATCCGCGTCATCCGCGAGGCCGTGGCTGAAGCGGA
GCGGCCGGTGATGCTCTACTCCATCGGCAAGGACTCATCGGTCATGCTGCACCTGGCCCTCAAGGCCTTCTACCCGTTGA
CGCTCCCCTTTCCGCTCCTGCACGTGGATACCACCTGGAAGTTCCGCGAGATGATCTCCTTCCGCGAGGAGACCGTGGCC
CGTCTGGGGCTGCGGCTGGTCAGCCACACCAACCACAAGGGGCTTAAGCAAGGGATCACCCCCTTCAGCCATGGCAGCCG
CGTCTATACCGATATCATGAAGACCGAGGCCCTGCGTGCGGTCCTTGACACGCAGGGTTTCGACATGATCTTCGGTGGCG
CCCGGCGCGACGAGGAGCGGAGCCGGGCCAAGGAACGTGTGTTTTCCTTCCGCGACAGTAATCATCGCTGGGAACCGCGC
AGCCAGCGGCCGGAACTCTGGAACATCCCCAACCTGAGGAAGAACCCGGGGGAAAGCTTCAGGGTCTTCCCCCTTTCCAA
CTGGACCGAGCTGGATGTGTGGCGCTATATCGCCCGGGAGCGGATCCCGGTGGTGCCGCTCTATTTTGCGGCGGAACGCC
CCGTGGTGTGGCGGGACGGCCAGTGGATCATGGTGGATGACGATCGGATGCGTCTGGAGCAGGGTGAGAGTCCCCAACTG
CGCATGATCCGTTTCAGGACTCTGGGATGCTACCCCCTTACGGCCGGGGTGGAATCGTTGGCCGGCACGGTGGAGGAGGT
CATTCGCGAGTTGGAACAGACCAGGCTTTCGGAGCGCTGCGGTCGCCTGATCGACTTTGACCAAGCCGGCAGCATGGAGA
AGAAGAAACAGGAGGGGTACTTCTGA

Upstream 100 bases:

>100_bases
GAATTCATGCCAGCCTATACTTTGCCTTCGAGTGGTGATCGACGGTATACAGCCATGTCGTTCGGACGCCTTTCACTAAT
CATCTTGGAAAACTTTCGCC

Downstream 100 bases:

>100_bases
TGGTTGCTGGAGATGGCGTGCTGCGTTTTCTGGTCTGCGGGAGCGTGGATGACGGCAAGTCCACCCTGATCGGCCACCTG
CTGCATCTGACCGGAAATCT

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MNDHTDYLDILERESIRVIREAVAEAERPVMLYSIGKDSSVMLHLALKAFYPLTLPFPLLHVDTTWKFREMISFREETVA
RLGLRLVSHTNHKGLKQGITPFSHGSRVYTDIMKTEALRAVLDTQGFDMIFGGARRDEERSRAKERVFSFRDSNHRWEPR
SQRPELWNIPNLRKNPGESFRVFPLSNWTELDVWRYIARERIPVVPLYFAAERPVVWRDGQWIMVDDDRMRLEQGESPQL
RMIRFRTLGCYPLTAGVESLAGTVEEVIRELEQTRLSERCGRLIDFDQAGSMEKKKQEGYF

Sequences:

>Translated_301_residues
MNDHTDYLDILERESIRVIREAVAEAERPVMLYSIGKDSSVMLHLALKAFYPLTLPFPLLHVDTTWKFREMISFREETVA
RLGLRLVSHTNHKGLKQGITPFSHGSRVYTDIMKTEALRAVLDTQGFDMIFGGARRDEERSRAKERVFSFRDSNHRWEPR
SQRPELWNIPNLRKNPGESFRVFPLSNWTELDVWRYIARERIPVVPLYFAAERPVVWRDGQWIMVDDDRMRLEQGESPQL
RMIRFRTLGCYPLTAGVESLAGTVEEVIRELEQTRLSERCGRLIDFDQAGSMEKKKQEGYF
>Mature_301_residues
MNDHTDYLDILERESIRVIREAVAEAERPVMLYSIGKDSSVMLHLALKAFYPLTLPFPLLHVDTTWKFREMISFREETVA
RLGLRLVSHTNHKGLKQGITPFSHGSRVYTDIMKTEALRAVLDTQGFDMIFGGARRDEERSRAKERVFSFRDSNHRWEPR
SQRPELWNIPNLRKNPGESFRVFPLSNWTELDVWRYIARERIPVVPLYFAAERPVVWRDGQWIMVDDDRMRLEQGESPQL
RMIRFRTLGCYPLTAGVESLAGTVEEVIRELEQTRLSERCGRLIDFDQAGSMEKKKQEGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=295, Percent_Identity=63.3898305084746, Blast_Score=382, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 35323; Mature: 35323

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDHTDYLDILERESIRVIREAVAEAERPVMLYSIGKDSSVMLHLALKAFYPLTLPFPLL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCEEE
HVDTTWKFREMISFREETVARLGLRLVSHTNHKGLKQGITPFSHGSRVYTDIMKTEALRA
EECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHH
VLDTQGFDMIFGGARRDEERSRAKERVFSFRDSNHRWEPRSQRPELWNIPNLRKNPGESF
HHHCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
RVFPLSNWTELDVWRYIARERIPVVPLYFAAERPVVWRDGQWIMVDDDRMRLEQGESPQL
EEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCEEEECCCEEEECCCHHHHHCCCCCCE
RMIRFRTLGCYPLTAGVESLAGTVEEVIRELEQTRLSERCGRLIDFDQAGSMEKKKQEGY
EEEEEEECCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCHHHHHCCC
F
C
>Mature Secondary Structure
MNDHTDYLDILERESIRVIREAVAEAERPVMLYSIGKDSSVMLHLALKAFYPLTLPFPLL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCEEE
HVDTTWKFREMISFREETVARLGLRLVSHTNHKGLKQGITPFSHGSRVYTDIMKTEALRA
EECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHH
VLDTQGFDMIFGGARRDEERSRAKERVFSFRDSNHRWEPRSQRPELWNIPNLRKNPGESF
HHHCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
RVFPLSNWTELDVWRYIARERIPVVPLYFAAERPVVWRDGQWIMVDDDRMRLEQGESPQL
EEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCEEEECCCEEEECCCHHHHHCCCCCCE
RMIRFRTLGCYPLTAGVESLAGTVEEVIRELEQTRLSERCGRLIDFDQAGSMEKKKQEGY
EEEEEEECCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCHHHHHCCC
F
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA