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The map label for this gene is leuB [H]

Identifier: 118578493

GI number: 118578493

Start: 52814

End: 53902

Strand: Direct

Name: leuB [H]

Synonym: Ppro_0045

Alternate gene names: 118578493

Gene position: 52814-53902 (Clockwise)

Preceding gene: 118578492

Following gene: 118578494

Centisome position: 1.32

GC content: 64.0

Gene sequence:

>1089_bases
ATGTCGAAATCGTACAAAATCGCGGTCCTGGCCGGCGACGGCGTCGGTCCCGAGGTTATGGCCGAGGCGCTTAGAGTGCT
GGACTGCGTTGAGCAGAAGTATGGCGTCACCTTCCAGCGCACCCCGGCCAATGTGGGGGGCGCGGGCATCGACAACGAGG
GCAAGGCGTTGCCGGAGAGCACCGTGAATATCTGCCGGGCGTCGGACGCCATCCTGTTCGGTTCCGTGGGCGGCCCCAAG
TGGGAGAACCTGCCGGCTGAAGAGCAGCCCGAGCGGGGCGCGCTTTTGCCGCTGCGCAAGATCTTCGGCCTGTACGCCAA
CCTGCGGCCGGCCATCATTTTTCCGTCCCTCACCGGCGCCTCGTCCCTGAAGGAAGAGGTGATTGCCGGCGGCTTCAACG
TGCTGGTGGTGCGCGAACTCACCGGCGGGATCTACTTCTCCCAGCCCAAGGGGATCGAGGGAGAGGGGCGTGAACGGGTC
GGCTTCGACACCCTGCGCTACAGCGTGCCCGAGATCGAGCGCATCACCCACGTGGCCTTCCAGGCCGCCCGGAAGCGGGG
CAAGAAGGTCTGCTCCATCGACAAGGCCAACGTGCTCTCATCGTCGGTGCTCTGGCGCGAGATCGTCACCACCATCTCCA
AGGAGTACCCGGACGTGGAGCTGTCCCACATGTACGTGGACAACGCTGCCATGCAACTGGTCAAGTGGCCCAAGCAGTTC
GACGTGATCCTGTGCGAGAACATGTTCGGCGACATCCTCTCCGATGAGGCGGCCATGCTCACCGGCTCCCTGGGCATGCT
CCCCAGCGCCTCCCTGGCCGAGGGCTCCTTCGGCATGTACGAGCCGTCCGGCGGTTCGGCACCGGACATCGCCGGCCAGG
GGATTGCAAACCCCATCGCCCAGATTCTCTCCACCGCCATGATGCTCAAGTTCTCCTTCGGCATGCTGGAGGCGGCCGAT
GCCATCGACAGCGCCGTTGCAACGGTTCTGGAGCAGGGATACCGCACCCATGACATCCATCAGAACAAGCCGGGGGAGAA
GCTGGTCAACACGAAAGAGATGGGCGACGCGATTATCGCTGTGCTCTGA

Upstream 100 bases:

>100_bases
AAGCGCTGACCGTACGCTGAATCCGTATTCCGTTTGACGGAACAGGGCAAGGCCGGGGCGGTTGCATCAACCGCCCTGTT
TTTATTACCAAGGAGAAATC

Downstream 100 bases:

>100_bases
GCGCGGCAGAAAAATTCACTTCAGAAAAGGAAGACAAGCAATGAAAGTCGGACTGGTCGGTTGGCGTGGCATGGTTGGCT
CGGTACTCCTTCAGCGCATG

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MSKSYKIAVLAGDGVGPEVMAEALRVLDCVEQKYGVTFQRTPANVGGAGIDNEGKALPESTVNICRASDAILFGSVGGPK
WENLPAEEQPERGALLPLRKIFGLYANLRPAIIFPSLTGASSLKEEVIAGGFNVLVVRELTGGIYFSQPKGIEGEGRERV
GFDTLRYSVPEIERITHVAFQAARKRGKKVCSIDKANVLSSSVLWREIVTTISKEYPDVELSHMYVDNAAMQLVKWPKQF
DVILCENMFGDILSDEAAMLTGSLGMLPSASLAEGSFGMYEPSGGSAPDIAGQGIANPIAQILSTAMMLKFSFGMLEAAD
AIDSAVATVLEQGYRTHDIHQNKPGEKLVNTKEMGDAIIAVL

Sequences:

>Translated_362_residues
MSKSYKIAVLAGDGVGPEVMAEALRVLDCVEQKYGVTFQRTPANVGGAGIDNEGKALPESTVNICRASDAILFGSVGGPK
WENLPAEEQPERGALLPLRKIFGLYANLRPAIIFPSLTGASSLKEEVIAGGFNVLVVRELTGGIYFSQPKGIEGEGRERV
GFDTLRYSVPEIERITHVAFQAARKRGKKVCSIDKANVLSSSVLWREIVTTISKEYPDVELSHMYVDNAAMQLVKWPKQF
DVILCENMFGDILSDEAAMLTGSLGMLPSASLAEGSFGMYEPSGGSAPDIAGQGIANPIAQILSTAMMLKFSFGMLEAAD
AIDSAVATVLEQGYRTHDIHQNKPGEKLVNTKEMGDAIIAVL
>Mature_361_residues
SKSYKIAVLAGDGVGPEVMAEALRVLDCVEQKYGVTFQRTPANVGGAGIDNEGKALPESTVNICRASDAILFGSVGGPKW
ENLPAEEQPERGALLPLRKIFGLYANLRPAIIFPSLTGASSLKEEVIAGGFNVLVVRELTGGIYFSQPKGIEGEGRERVG
FDTLRYSVPEIERITHVAFQAARKRGKKVCSIDKANVLSSSVLWREIVTTISKEYPDVELSHMYVDNAAMQLVKWPKQFD
VILCENMFGDILSDEAAMLTGSLGMLPSASLAEGSFGMYEPSGGSAPDIAGQGIANPIAQILSTAMMLKFSFGMLEAADA
IDSAVATVLEQGYRTHDIHQNKPGEKLVNTKEMGDAIIAVL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=335, Percent_Identity=30.4477611940298, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI4758582, Length=343, Percent_Identity=28.8629737609329, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI28178838, Length=329, Percent_Identity=28.8753799392097, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI28178816, Length=313, Percent_Identity=28.4345047923323, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI28178821, Length=313, Percent_Identity=28.4345047923323, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI28178819, Length=142, Percent_Identity=35.2112676056338, Blast_Score=89, Evalue=9e-18,
Organism=Escherichia coli, GI87081683, Length=358, Percent_Identity=61.1731843575419, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI1788101, Length=373, Percent_Identity=34.0482573726542, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1787381, Length=399, Percent_Identity=25.062656641604, Blast_Score=85, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI71986051, Length=330, Percent_Identity=30.9090909090909, Blast_Score=137, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI25144293, Length=353, Percent_Identity=31.728045325779, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17505779, Length=356, Percent_Identity=28.3707865168539, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17550882, Length=350, Percent_Identity=30.5714285714286, Blast_Score=114, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6319830, Length=369, Percent_Identity=45.5284552845528, Blast_Score=270, Evalue=4e-73,
Organism=Saccharomyces cerevisiae, GI6322097, Length=361, Percent_Identity=35.4570637119114, Blast_Score=166, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6324709, Length=347, Percent_Identity=30.835734870317, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6324291, Length=342, Percent_Identity=30.7017543859649, Blast_Score=128, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24643270, Length=352, Percent_Identity=30.9659090909091, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24643268, Length=352, Percent_Identity=30.9659090909091, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24661184, Length=345, Percent_Identity=28.9855072463768, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI161078635, Length=342, Percent_Identity=28.0701754385965, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI161078633, Length=341, Percent_Identity=26.3929618768328, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24650122, Length=341, Percent_Identity=26.3929618768328, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI161078637, Length=342, Percent_Identity=28.0701754385965, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI161078639, Length=248, Percent_Identity=30.241935483871, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI281362242, Length=342, Percent_Identity=26.3157894736842, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24648872, Length=342, Percent_Identity=26.3157894736842, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38845; Mature: 38714

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSYKIAVLAGDGVGPEVMAEALRVLDCVEQKYGVTFQRTPANVGGAGIDNEGKALPES
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCHH
TVNICRASDAILFGSVGGPKWENLPAEEQPERGALLPLRKIFGLYANLRPAIIFPSLTGA
HHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCH
SSLKEEVIAGGFNVLVVRELTGGIYFSQPKGIEGEGRERVGFDTLRYSVPEIERITHVAF
HHHHHHHHHCCCEEEEEEECCCCEEECCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHH
QAARKRGKKVCSIDKANVLSSSVLWREIVTTISKEYPDVELSHMYVDNAAMQLVKWPKQF
HHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHEEEHHHHHHHHHHCCHHH
DVILCENMFGDILSDEAAMLTGSLGMLPSASLAEGSFGMYEPSGGSAPDIAGQGIANPIA
CEEEHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
QILSTAMMLKFSFGMLEAADAIDSAVATVLEQGYRTHDIHQNKPGEKLVNTKEMGDAIIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCHHHHCCEEEE
VL
EC
>Mature Secondary Structure 
SKSYKIAVLAGDGVGPEVMAEALRVLDCVEQKYGVTFQRTPANVGGAGIDNEGKALPES
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCHH
TVNICRASDAILFGSVGGPKWENLPAEEQPERGALLPLRKIFGLYANLRPAIIFPSLTGA
HHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCH
SSLKEEVIAGGFNVLVVRELTGGIYFSQPKGIEGEGRERVGFDTLRYSVPEIERITHVAF
HHHHHHHHHCCCEEEEEEECCCCEEECCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHH
QAARKRGKKVCSIDKANVLSSSVLWREIVTTISKEYPDVELSHMYVDNAAMQLVKWPKQF
HHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHEEEHHHHHHHHHHCCHHH
DVILCENMFGDILSDEAAMLTGSLGMLPSASLAEGSFGMYEPSGGSAPDIAGQGIANPIA
CEEEHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
QILSTAMMLKFSFGMLEAADAIDSAVATVLEQGYRTHDIHQNKPGEKLVNTKEMGDAIIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCHHHHCCEEEE
VL
EC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA