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The map label for this gene is metK [H]

Identifier: 118578492

GI number: 118578492

Start: 51547

End: 52713

Strand: Direct

Name: metK [H]

Synonym: Ppro_0044

Alternate gene names: 118578492

Gene position: 51547-52713 (Clockwise)

Preceding gene: 118578491

Following gene: 118578493

Centisome position: 1.29

GC content: 61.95

Gene sequence:

>1167_bases
ATGGCTGAAAAATACATCTTTACATCCGAGTCGGTATCCGAAGGGCATCCCGACAAGGTTGCCGACCAGATCTCCGATGC
GGTGCTGGATGCGATCCTGGAGCAGGACACCAGTGCCCGCGTCGCATGTGAAACCATGGTGACCACCGGCATGGTGGTCG
TTGCCGGCGAGATAACCACATCCGCCAACATCGATTTTCCCGCCACCATTCGTCAGACCGTCAAGGAGATCGGCTATAAC
GATTCCGCCATGGGTTTCGACTACGAGACCTGTGCCGTGCTGACCTCCATCGACAAGCAGTCTCCCGACATCTCCCAGGG
GGTCACCGAGGGGGACGGCCTGTACAAGGAGCAGGGGGCAGGCGACCAGGGGTTGATGTTCGGCTATGCCTGCAACGAGA
CTCCCGAACTGATGCCCATGCCGATCCAGCTCTCCCACCAGTTGGTGAAGAAACTGGCCGATGTGCGCAAGTCCGGGCAG
TTGAGCTTTCTCCGTCCCGATTCCAAATCCCAGGTGTCGGTTGAGTACTTGAACGACAAGCCGGCCCGCATCGACACCGT
GGTGATCTCCACCCAGCACGCCCCGGACGTGACCCACAAACAGATCGAGGAGCAGGTCATCGAAGAGGTGGTCAAGAAGG
TCATCCCGGCCGAGCTCTTGGACAGCGCCACCCGCTACTTCATCAACCCCACCGGCCGTTTCGTGGTCGGCGGCCCCATG
GGCGACTGCGGCCTGACCGGCCGCAAAATCATCGTCGATACCTATGGCGGCATGGGCCGCCACGGCGGCGGCGCCTTTTC
GGGCAAGGACCCATCCAAGGTGGACCGCTCGGCAGCCTACATGGGGCGCTATGTGGCCAAGAACCTGGTGGCGGCCGGCC
TGTGCGACCGCTGCGAGGTGCAGGTGGCCTATGCCATCGGCGTGGCCCAGCCGGTCTCCATCATGGTGCACACCTTCGGC
ACCGGCAGGGTCTCGGAAGATCGACTGGCCGAACTGGTGCGCGAGGTGTTCGACATGCGTCCCCGGGCCATCATCGAGCA
GCTGGACCTGCTGCGCCCGGTCTACAAGAAAACCGCCGCCTACGGTCATTTCGGTCGGGAACTGCCGGAATTCACCTGGG
AGAAGACCGACAAGGCCGCCATCCTCAAGGAAAAAGCCGCTTGCTGA

Upstream 100 bases:

>100_bases
GGCACCTGCCTATAAATTAAACAGTCTTATTTCGCCGGAACGGCTCGGTTGAATATCCGCCGATCAAACATATTACGGAA
AACGGGAGGAGTAACCCATC

Downstream 100 bases:

>100_bases
AAGCGCTGACCGTACGCTGAATCCGTATTCCGTTTGACGGAACAGGGCAAGGCCGGGGCGGTTGCATCAACCGCCCTGTT
TTTATTACCAAGGAGAAATC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MAEKYIFTSESVSEGHPDKVADQISDAVLDAILEQDTSARVACETMVTTGMVVVAGEITTSANIDFPATIRQTVKEIGYN
DSAMGFDYETCAVLTSIDKQSPDISQGVTEGDGLYKEQGAGDQGLMFGYACNETPELMPMPIQLSHQLVKKLADVRKSGQ
LSFLRPDSKSQVSVEYLNDKPARIDTVVISTQHAPDVTHKQIEEQVIEEVVKKVIPAELLDSATRYFINPTGRFVVGGPM
GDCGLTGRKIIVDTYGGMGRHGGGAFSGKDPSKVDRSAAYMGRYVAKNLVAAGLCDRCEVQVAYAIGVAQPVSIMVHTFG
TGRVSEDRLAELVREVFDMRPRAIIEQLDLLRPVYKKTAAYGHFGRELPEFTWEKTDKAAILKEKAAC

Sequences:

>Translated_388_residues
MAEKYIFTSESVSEGHPDKVADQISDAVLDAILEQDTSARVACETMVTTGMVVVAGEITTSANIDFPATIRQTVKEIGYN
DSAMGFDYETCAVLTSIDKQSPDISQGVTEGDGLYKEQGAGDQGLMFGYACNETPELMPMPIQLSHQLVKKLADVRKSGQ
LSFLRPDSKSQVSVEYLNDKPARIDTVVISTQHAPDVTHKQIEEQVIEEVVKKVIPAELLDSATRYFINPTGRFVVGGPM
GDCGLTGRKIIVDTYGGMGRHGGGAFSGKDPSKVDRSAAYMGRYVAKNLVAAGLCDRCEVQVAYAIGVAQPVSIMVHTFG
TGRVSEDRLAELVREVFDMRPRAIIEQLDLLRPVYKKTAAYGHFGRELPEFTWEKTDKAAILKEKAAC
>Mature_387_residues
AEKYIFTSESVSEGHPDKVADQISDAVLDAILEQDTSARVACETMVTTGMVVVAGEITTSANIDFPATIRQTVKEIGYND
SAMGFDYETCAVLTSIDKQSPDISQGVTEGDGLYKEQGAGDQGLMFGYACNETPELMPMPIQLSHQLVKKLADVRKSGQL
SFLRPDSKSQVSVEYLNDKPARIDTVVISTQHAPDVTHKQIEEQVIEEVVKKVIPAELLDSATRYFINPTGRFVVGGPMG
DCGLTGRKIIVDTYGGMGRHGGGAFSGKDPSKVDRSAAYMGRYVAKNLVAAGLCDRCEVQVAYAIGVAQPVSIMVHTFGT
GRVSEDRLAELVREVFDMRPRAIIEQLDLLRPVYKKTAAYGHFGRELPEFTWEKTDKAAILKEKAAC

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=374, Percent_Identity=57.7540106951872, Blast_Score=430, Evalue=1e-120,
Organism=Homo sapiens, GI4557737, Length=374, Percent_Identity=58.0213903743316, Blast_Score=429, Evalue=1e-120,
Organism=Escherichia coli, GI1789311, Length=383, Percent_Identity=68.668407310705, Blast_Score=546, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI17538498, Length=377, Percent_Identity=58.3554376657825, Blast_Score=443, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI17538494, Length=377, Percent_Identity=57.8249336870027, Blast_Score=441, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI25145633, Length=386, Percent_Identity=57.5129533678757, Blast_Score=441, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17551082, Length=394, Percent_Identity=55.3299492385787, Blast_Score=432, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI32565905, Length=377, Percent_Identity=52.7851458885942, Blast_Score=376, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI32565911, Length=329, Percent_Identity=56.2310030395137, Blast_Score=373, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI25145635, Length=341, Percent_Identity=55.1319648093842, Blast_Score=370, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI32565903, Length=302, Percent_Identity=56.6225165562914, Blast_Score=337, Evalue=7e-93,
Organism=Caenorhabditis elegans, GI32565909, Length=84, Percent_Identity=59.5238095238095, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI32565907, Length=40, Percent_Identity=77.5, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320710, Length=381, Percent_Identity=56.6929133858268, Blast_Score=445, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6323209, Length=379, Percent_Identity=56.7282321899736, Blast_Score=442, Evalue=1e-125,
Organism=Drosophila melanogaster, GI45552159, Length=375, Percent_Identity=54.9333333333333, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580529, Length=375, Percent_Identity=54.9333333333333, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580537, Length=375, Percent_Identity=54.9333333333333, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580533, Length=375, Percent_Identity=54.9333333333333, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580535, Length=375, Percent_Identity=54.9333333333333, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580531, Length=375, Percent_Identity=54.9333333333333, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580527, Length=378, Percent_Identity=53.968253968254, Blast_Score=396, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580525, Length=378, Percent_Identity=53.968253968254, Blast_Score=396, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580541, Length=365, Percent_Identity=53.1506849315069, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580539, Length=122, Percent_Identity=53.2786885245902, Blast_Score=138, Evalue=7e-33,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 42259; Mature: 42127

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKYIFTSESVSEGHPDKVADQISDAVLDAILEQDTSARVACETMVTTGMVVVAGEITT
CCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEEEECEEC
SANIDFPATIRQTVKEIGYNDSAMGFDYETCAVLTSIDKQSPDISQGVTEGDGLYKEQGA
CCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHCCCCCCCCCHHCCC
GDQGLMFGYACNETPELMPMPIQLSHQLVKKLADVRKSGQLSFLRPDSKSQVSVEYLNDK
CCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEECCCC
PARIDTVVISTQHAPDVTHKQIEEQVIEEVVKKVIPAELLDSATRYFINPTGRFVVGGPM
CCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEECCCCCEEECCCC
GDCGLTGRKIIVDTYGGMGRHGGGAFSGKDPSKVDRSAAYMGRYVAKNLVAAGLCDRCEV
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHE
QVAYAIGVAQPVSIMVHTFGTGRVSEDRLAELVREVFDMRPRAIIEQLDLLRPVYKKTAA
EEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
YGHFGRELPEFTWEKTDKAAILKEKAAC
HHHHHHHCCCHHHCCCCHHHHHHHHCCC
>Mature Secondary Structure 
AEKYIFTSESVSEGHPDKVADQISDAVLDAILEQDTSARVACETMVTTGMVVVAGEITT
CCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEEEECEEC
SANIDFPATIRQTVKEIGYNDSAMGFDYETCAVLTSIDKQSPDISQGVTEGDGLYKEQGA
CCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHCCCCCCCCCHHCCC
GDQGLMFGYACNETPELMPMPIQLSHQLVKKLADVRKSGQLSFLRPDSKSQVSVEYLNDK
CCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEECCCC
PARIDTVVISTQHAPDVTHKQIEEQVIEEVVKKVIPAELLDSATRYFINPTGRFVVGGPM
CCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEECCCCCEEECCCC
GDCGLTGRKIIVDTYGGMGRHGGGAFSGKDPSKVDRSAAYMGRYVAKNLVAAGLCDRCEV
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHE
QVAYAIGVAQPVSIMVHTFGTGRVSEDRLAELVREVFDMRPRAIIEQLDLLRPVYKKTAA
EEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
YGHFGRELPEFTWEKTDKAAILKEKAAC
HHHHHHHCCCHHHCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA