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The map label for this gene is nemA [H]

Identifier: 118478883

GI number: 118478883

Start: 3479547

End: 3480740

Strand: Reverse

Name: nemA [H]

Synonym: BALH_3282

Alternate gene names: 118478883

Gene position: 3480740-3479547 (Counterclockwise)

Preceding gene: 118478884

Following gene: 118478882

Centisome position: 66.21

GC content: 37.94

Gene sequence:

>1194_bases
TTGACGTTACTATCTAAAAGTAAGTATGATGCGATTGTAGTAAGAATTTTTTTAGGGGGAAGAAAAATGACAAGTTCAAA
TAATAAAGCGGCAAGTATTTATGAAGGAACAAATGTAAATGCTGGGGGATCTTTTGTAAGTATTGAAGAAACGAAAATTT
TTAATCCAATTCAAATTGGATCTTGGCCTCTTCGTAACCGTATAGCAATGGCACCAATGACAAGATGCTTTGCGGACAAT
GAAACTGGAGCGGTGGGTGCTGATGTAGTGGAATACTATAGAAAACGTGCAGTTGATGGAGTTGGATTAATTATTACAGA
AGGAATTGTTATTAGCGCAAGAGCAAAAGGAAACCCAGGGGTACCAGGAATTTATACACAAGAACAAATTGATTCGTGGA
AACCTGTTACAGAGGCAGTACATAAAGAAGGCGGGACAATTATTGCTCAAATATGGCATGTTGGTCGTATGAGTCATCAT
GAAATAATTGGTGGGCAAATGCCGCAAGCACCGTCTGCAATCGCTGCAGAGGGAAATGTTCCACGTTTTCGCAAACCATT
CGATACACCAGAAGCAATGACATTAGAAGAAATACAAGAAGTTATTAGTCAATACGCACAAGCTGCGAAAAATGCGATTG
AAGCTGGGTTTGATGGAGTAGAAATTCACGGTGCACACGGATATTTAATTGATCAATTTACTTATGAGTTTGCAAATAAG
CGTACAGATAAATATGGCGGTGACTTAAAGCAAAGGTTAACGTTTATGAAAGAAGTAACTGAGGCTGTAATAGAAGCAAT
TGGAGCTGATAAAACACTTCTTCGTTTCTCTGCTTTTAAAGGTGATAATCCTACTTATATGTGGGAGAACCCTGAACTGG
CAATTGAAACATTCGTAAATATGTTTAAAGAAGTTGGATTAACGATGATTCACCCTTCAACGATGAGTTATACGCAAGTG
ATTGCTGACGGTAAGAATTTTCATCAATTAGTAAGAAAATATTGGGATGGTGCGATTGTTGGGGTTGGTAATTTGAATCC
GAAAGAGGCAGAAGAAGCGTTGCAAGAAGGAACAATTGATGTAGCTGCATTCGGAAGACCTTTAATTGCTAATCCGGATT
TTGTTCATCGAATTAAAAGTGCTGAAAGTTTAGTTGAATATGACGCGAAAGAGCATCTTGCTACATTGATTTGA

Upstream 100 bases:

>100_bases
TGGTAAATAAAAAAGGTTTCCTACTCAGGAAATCTTTTTTATTTATTTATAGCATCCCATTTGTTTAATATTCGAATAAT
CTCTGGTTAAAGCGTATAAA

Downstream 100 bases:

>100_bases
ATAAAGATAAAAGGAGAACACACGGAGTGTTCTCCTTTTATTATGGGATTTCATAGGGGGAAGCGAGATGAGTGAAACGC
TACTCGTTATTATTAGTATA

Product: Oye family NADH-dependent flavin oxidoreductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MTLLSKSKYDAIVVRIFLGGRKMTSSNNKAASIYEGTNVNAGGSFVSIEETKIFNPIQIGSWPLRNRIAMAPMTRCFADN
ETGAVGADVVEYYRKRAVDGVGLIITEGIVISARAKGNPGVPGIYTQEQIDSWKPVTEAVHKEGGTIIAQIWHVGRMSHH
EIIGGQMPQAPSAIAAEGNVPRFRKPFDTPEAMTLEEIQEVISQYAQAAKNAIEAGFDGVEIHGAHGYLIDQFTYEFANK
RTDKYGGDLKQRLTFMKEVTEAVIEAIGADKTLLRFSAFKGDNPTYMWENPELAIETFVNMFKEVGLTMIHPSTMSYTQV
IADGKNFHQLVRKYWDGAIVGVGNLNPKEAEEALQEGTIDVAAFGRPLIANPDFVHRIKSAESLVEYDAKEHLATLI

Sequences:

>Translated_397_residues
MTLLSKSKYDAIVVRIFLGGRKMTSSNNKAASIYEGTNVNAGGSFVSIEETKIFNPIQIGSWPLRNRIAMAPMTRCFADN
ETGAVGADVVEYYRKRAVDGVGLIITEGIVISARAKGNPGVPGIYTQEQIDSWKPVTEAVHKEGGTIIAQIWHVGRMSHH
EIIGGQMPQAPSAIAAEGNVPRFRKPFDTPEAMTLEEIQEVISQYAQAAKNAIEAGFDGVEIHGAHGYLIDQFTYEFANK
RTDKYGGDLKQRLTFMKEVTEAVIEAIGADKTLLRFSAFKGDNPTYMWENPELAIETFVNMFKEVGLTMIHPSTMSYTQV
IADGKNFHQLVRKYWDGAIVGVGNLNPKEAEEALQEGTIDVAAFGRPLIANPDFVHRIKSAESLVEYDAKEHLATLI
>Mature_396_residues
TLLSKSKYDAIVVRIFLGGRKMTSSNNKAASIYEGTNVNAGGSFVSIEETKIFNPIQIGSWPLRNRIAMAPMTRCFADNE
TGAVGADVVEYYRKRAVDGVGLIITEGIVISARAKGNPGVPGIYTQEQIDSWKPVTEAVHKEGGTIIAQIWHVGRMSHHE
IIGGQMPQAPSAIAAEGNVPRFRKPFDTPEAMTLEEIQEVISQYAQAAKNAIEAGFDGVEIHGAHGYLIDQFTYEFANKR
TDKYGGDLKQRLTFMKEVTEAVIEAIGADKTLLRFSAFKGDNPTYMWENPELAIETFVNMFKEVGLTMIHPSTMSYTQVI
ADGKNFHQLVRKYWDGAIVGVGNLNPKEAEEALQEGTIDVAAFGRPLIANPDFVHRIKSAESLVEYDAKEHLATLI

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=341, Percent_Identity=39.0029325513196, Blast_Score=225, Evalue=5e-60,
Organism=Escherichia coli, GI1789463, Length=345, Percent_Identity=31.304347826087, Blast_Score=141, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI17565138, Length=349, Percent_Identity=28.6532951289398, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI72001454, Length=359, Percent_Identity=23.9554317548747, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17564188, Length=310, Percent_Identity=24.8387096774194, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17559804, Length=191, Percent_Identity=29.8429319371728, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17559802, Length=271, Percent_Identity=27.6752767527675, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17540738, Length=342, Percent_Identity=26.0233918128655, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17566914, Length=215, Percent_Identity=28.8372093023256, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6325086, Length=366, Percent_Identity=31.6939890710383, Blast_Score=166, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6321973, Length=364, Percent_Identity=31.3186813186813, Blast_Score=164, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 43716; Mature: 43585

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLSKSKYDAIVVRIFLGGRKMTSSNNKAASIYEGTNVNAGGSFVSIEETKIFNPIQIG
CCCCCCCCCCEEEEEEEECCCEECCCCCCEEEEECCCCCCCCCCEEEEEHHHCCCCEECC
SWPLRNRIAMAPMTRCFADNETGAVGADVVEYYRKRAVDGVGLIITEGIVISARAKGNPG
CCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCEEEEEECCCCCC
VPGIYTQEQIDSWKPVTEAVHKEGGTIIAQIWHVGRMSHHEIIGGQMPQAPSAIAAEGNV
CCCCCCHHHHHCCCHHHHHHHHCCCEEEEHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCC
PRFRKPFDTPEAMTLEEIQEVISQYAQAAKNAIEAGFDGVEIHGAHGYLIDQFTYEFANK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEHHHHHHHHHHC
RTDKYGGDLKQRLTFMKEVTEAVIEAIGADKTLLRFSAFKGDNPTYMWENPELAIETFVN
CCHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEECCCCCCCEEECCCCHHHHHHHH
MFKEVGLTMIHPSTMSYTQVIADGKNFHQLVRKYWDGAIVGVGNLNPKEAEEALQEGTID
HHHHCCEEEECCCCHHHHHHHHCCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCEE
VAAFGRPLIANPDFVHRIKSAESLVEYDAKEHLATLI
EECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TLLSKSKYDAIVVRIFLGGRKMTSSNNKAASIYEGTNVNAGGSFVSIEETKIFNPIQIG
CCCCCCCCCEEEEEEEECCCEECCCCCCEEEEECCCCCCCCCCEEEEEHHHCCCCEECC
SWPLRNRIAMAPMTRCFADNETGAVGADVVEYYRKRAVDGVGLIITEGIVISARAKGNPG
CCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCEEEEEECCCCCC
VPGIYTQEQIDSWKPVTEAVHKEGGTIIAQIWHVGRMSHHEIIGGQMPQAPSAIAAEGNV
CCCCCCHHHHHCCCHHHHHHHHCCCEEEEHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCC
PRFRKPFDTPEAMTLEEIQEVISQYAQAAKNAIEAGFDGVEIHGAHGYLIDQFTYEFANK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEHHHHHHHHHHC
RTDKYGGDLKQRLTFMKEVTEAVIEAIGADKTLLRFSAFKGDNPTYMWENPELAIETFVN
CCHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEECCCCCCCEEECCCCHHHHHHHH
MFKEVGLTMIHPSTMSYTQVIADGKNFHQLVRKYWDGAIVGVGNLNPKEAEEALQEGTID
HHHHCCEEEECCCCHHHHHHHHCCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCEE
VAAFGRPLIANPDFVHRIKSAESLVEYDAKEHLATLI
EECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]