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The map label for this gene is phaB [H]

Identifier: 118477762

GI number: 118477762

Start: 2247930

End: 2248985

Strand: Reverse

Name: phaB [H]

Synonym: BALH_2100

Alternate gene names: 118477762

Gene position: 2248985-2247930 (Counterclockwise)

Preceding gene: 118477767

Following gene: 118477761

Centisome position: 42.78

GC content: 35.23

Gene sequence:

>1056_bases
ATGACTGAACACGTTTTATTTTCTGTTAGCGAAAACGGCGTTGCATCAATTACTTTAAACCGCCCAAAGGCACTTAATTC
TTTATCTTATGACATGCTGCAACCAATTGGGCAAAAATTGAAAGAATGGGAACATGATGAGCGAATTGCGCTAATCGTAT
TAAAGGGAGCTGGCACAAAAGGTTTTTGTGCAGGCGGCGACATTAAAACCCTTTATGAAGCACGTTCAAATGAAGCTGCA
TTACAGCATGCGGAACGCTTCTTTGAAGAAGAGTATGAAATTGATACATATATTTATCAATACAAAAAACCGATCATCGC
TTGTTTAGATGGAATTGTAATGGGTGGTGGTGTCGGTCTTACAAATGGTGCGAAATACCGAATTGTAACCGAGCGTACAA
AATGGGCAATGCCAGAGATGAATATCGGCTTCTTCCCAGACGTCGGCGCTGCTTATTTCTTAAATAAAGCTCCAGGGTTC
GCTGGGAGATATGTTGCTTTAACAGCATCTATTTTAAAAGCTTCTGACGTATTATTTATAAACGCTGCTGATTATTTTAT
GACATCTGATTCATTACCAAATTTCCTTGCTAAACTTGAAAGTGTAAATTGGCATAAAGAAGATGATGTACATACTCATT
TAAAAGAAGTTATTCGTACATTTGCAACTACTTCAAATTTAGAAAGCGAGCTTGCTCCATTATTAGAAGAAATCAATGCA
CATTTTGTTTTCGATACAATTGAAGAAATTATTCATTCATTAGAGAAAGATCAAAGTTCCTTTGCTTTAAAAACGAAAGA
AACACTATTATCAAAATCCCCTATTTCATTAAAAGTAACATTAAAACAGTTTATTGATGGACATGATAAGTCCGTTGAAG
AATGTTTCGCTACTGACCTTGTACTTGCTAAGAATTTCATGAGACATGAAGATTTCTTTGAAGGAGTACGATCCGTTGTC
GTTGATAAAGATCAAAATCCCAATTATAAATATAAGCAGTTAAGTGATGTTTCAGAAGAAGATGTAAATCGGTTCTTTAA
CTTACTTAATGCATAA

Upstream 100 bases:

>100_bases
GAAATTAACATGTACCGCCAACTTTAGACAAAATTTATAAAGGACATTTCAGTACGTATCAAGAATTAAGAATAAGAAGA
TATGTAAGGGAGGATGAATT

Downstream 100 bases:

>100_bases
CCACTCGCCCGTTCATTTGGGATAAACCTGAGGCTATATAGTCGTGTAATTCCATGACTATATAGCCTCTTTTCTATTTA
ATATTTTTTAATAAAATTTA

Product: 3-hydroxyisobutyryl-CoA hydrolase

Products: NA

Alternate protein names: Crotonase [H]

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MTEHVLFSVSENGVASITLNRPKALNSLSYDMLQPIGQKLKEWEHDERIALIVLKGAGTKGFCAGGDIKTLYEARSNEAA
LQHAERFFEEEYEIDTYIYQYKKPIIACLDGIVMGGGVGLTNGAKYRIVTERTKWAMPEMNIGFFPDVGAAYFLNKAPGF
AGRYVALTASILKASDVLFINAADYFMTSDSLPNFLAKLESVNWHKEDDVHTHLKEVIRTFATTSNLESELAPLLEEINA
HFVFDTIEEIIHSLEKDQSSFALKTKETLLSKSPISLKVTLKQFIDGHDKSVEECFATDLVLAKNFMRHEDFFEGVRSVV
VDKDQNPNYKYKQLSDVSEEDVNRFFNLLNA

Sequences:

>Translated_351_residues
MTEHVLFSVSENGVASITLNRPKALNSLSYDMLQPIGQKLKEWEHDERIALIVLKGAGTKGFCAGGDIKTLYEARSNEAA
LQHAERFFEEEYEIDTYIYQYKKPIIACLDGIVMGGGVGLTNGAKYRIVTERTKWAMPEMNIGFFPDVGAAYFLNKAPGF
AGRYVALTASILKASDVLFINAADYFMTSDSLPNFLAKLESVNWHKEDDVHTHLKEVIRTFATTSNLESELAPLLEEINA
HFVFDTIEEIIHSLEKDQSSFALKTKETLLSKSPISLKVTLKQFIDGHDKSVEECFATDLVLAKNFMRHEDFFEGVRSVV
VDKDQNPNYKYKQLSDVSEEDVNRFFNLLNA
>Mature_350_residues
TEHVLFSVSENGVASITLNRPKALNSLSYDMLQPIGQKLKEWEHDERIALIVLKGAGTKGFCAGGDIKTLYEARSNEAAL
QHAERFFEEEYEIDTYIYQYKKPIIACLDGIVMGGGVGLTNGAKYRIVTERTKWAMPEMNIGFFPDVGAAYFLNKAPGFA
GRYVALTASILKASDVLFINAADYFMTSDSLPNFLAKLESVNWHKEDDVHTHLKEVIRTFATTSNLESELAPLLEEINAH
FVFDTIEEIIHSLEKDQSSFALKTKETLLSKSPISLKVTLKQFIDGHDKSVEECFATDLVLAKNFMRHEDFFEGVRSVVV
DKDQNPNYKYKQLSDVSEEDVNRFFNLLNA

Specific function: Converts O-Succinylbenzoyl-CoA (Osb-CoA) To 1,4- Dihydroxy-2-Naphthoic Acid (Dhna). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI37594471, Length=339, Percent_Identity=41.0029498525074, Blast_Score=262, Evalue=3e-70,
Organism=Homo sapiens, GI37594469, Length=295, Percent_Identity=39.6610169491525, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI194097323, Length=148, Percent_Identity=35.1351351351351, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI1788597, Length=258, Percent_Identity=23.6434108527132, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI221142681, Length=149, Percent_Identity=30.2013422818792, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787660, Length=174, Percent_Identity=32.7586206896552, Blast_Score=68, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI25144160, Length=337, Percent_Identity=38.8724035608309, Blast_Score=237, Evalue=6e-63,
Organism=Caenorhabditis elegans, GI25144157, Length=337, Percent_Identity=38.8724035608309, Blast_Score=237, Evalue=6e-63,
Organism=Caenorhabditis elegans, GI17554946, Length=146, Percent_Identity=33.5616438356164, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25145438, Length=156, Percent_Identity=33.974358974359, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17540714, Length=183, Percent_Identity=22.9508196721311, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17534483, Length=178, Percent_Identity=25.2808988764045, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=350, Percent_Identity=32.2857142857143, Blast_Score=169, Evalue=6e-43,
Organism=Drosophila melanogaster, GI28571730, Length=333, Percent_Identity=40.8408408408408, Blast_Score=256, Evalue=1e-68,
Organism=Drosophila melanogaster, GI28571729, Length=333, Percent_Identity=40.8408408408408, Blast_Score=256, Evalue=1e-68,
Organism=Drosophila melanogaster, GI20129971, Length=241, Percent_Identity=28.2157676348548, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24653477, Length=241, Percent_Identity=28.2157676348548, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24653139, Length=177, Percent_Identity=27.683615819209, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.55 [H]

Molecular weight: Translated: 39578; Mature: 39447

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEHVLFSVSENGVASITLNRPKALNSLSYDMLQPIGQKLKEWEHDERIALIVLKGAGTK
CCCCEEEEECCCCEEEEEECCCHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCC
GFCAGGDIKTLYEARSNEAALQHAERFFEEEYEIDTYIYQYKKPIIACLDGIVMGGGVGL
CCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TNGAKYRIVTERTKWAMPEMNIGFFPDVGAAYFLNKAPGFAGRYVALTASILKASDVLFI
CCCCEEEEEECCCHHCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEE
NAADYFMTSDSLPNFLAKLESVNWHKEDDVHTHLKEVIRTFATTSNLESELAPLLEEINA
ECHHHHEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
HFVFDTIEEIIHSLEKDQSSFALKTKETLLSKSPISLKVTLKQFIDGHDKSVEECFATDL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEHHHHCCCCCCHHHHHHHHHH
VLAKNFMRHEDFFEGVRSVVVDKDQNPNYKYKQLSDVSEEDVNRFFNLLNA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TEHVLFSVSENGVASITLNRPKALNSLSYDMLQPIGQKLKEWEHDERIALIVLKGAGTK
CCCEEEEECCCCEEEEEECCCHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCC
GFCAGGDIKTLYEARSNEAALQHAERFFEEEYEIDTYIYQYKKPIIACLDGIVMGGGVGL
CCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TNGAKYRIVTERTKWAMPEMNIGFFPDVGAAYFLNKAPGFAGRYVALTASILKASDVLFI
CCCCEEEEEECCCHHCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEE
NAADYFMTSDSLPNFLAKLESVNWHKEDDVHTHLKEVIRTFATTSNLESELAPLLEEINA
ECHHHHEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
HFVFDTIEEIIHSLEKDQSSFALKTKETLLSKSPISLKVTLKQFIDGHDKSVEECFATDL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEHHHHCCCCCCHHHHHHHHHH
VLAKNFMRHEDFFEGVRSVVVDKDQNPNYKYKQLSDVSEEDVNRFFNLLNA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8655474; 11466286 [H]