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The map label for this gene is hisF [H]

Identifier: 118476971

GI number: 118476971

Start: 1413719

End: 1414477

Strand: Direct

Name: hisF [H]

Synonym: BALH_1265

Alternate gene names: 118476971

Gene position: 1413719-1414477 (Clockwise)

Preceding gene: 118476970

Following gene: 118476972

Centisome position: 26.89

GC content: 40.71

Gene sequence:

>759_bases
ATGTTAGCGAAACGCATTATTCCATGTCTAGATGTGAAAGAAGGGCGAGTCGTAAAGGGGGTAAATTTTATAGGGTTACA
AGATGTCGGTGATCCTGTTGAAATAGCAGCTTTATATAATGATGCGGGAGCAGATGAAATAGTATTTTTAGATATTACGG
CAACGCATGAAGGACGAAAAACAATTATAGATGTTGTAAAAAAAACTGCTTCAAAAGTATTTATTCCTCTTACAGTTGGC
GGAGGAATTTCAAGTGTGAAAGATATGTACAAATTACTAAGAGCGGGAGCAGATAAAGTTTCAATTAACTCAGCAGCAGT
GCGAAATCCAAAATTAATCGAAGAAGGGGCACAGCACTTTGGTTCACAATGTATTGTCGTAGCAATTGATGCTAGAAAAG
TAGCAGAAGGTAAGTGGAATGTATATGTGAACGGCGGAAGAGTTGATACGGGAATGGATGCAATCGAGTGGGCAAAGCGC
GTTGTTATGCTTGGTGCCGGCGAAATTTTGTTAACGAGTATGGATGCAGATGGAACGAAAAACGGATATGACCTTCGTTT
AACAGAAGAAATTTCAAAAAGTGTTTCCGTACCAGTCATCGCGTCAGGTGGATGTGGCCATGCTGATCACATTATAGAAG
TGTTTCAAAAGACAACAGTTGATGCAGCGTTAGCAGCATCCATCTTTCATTATGGCGAGGCGACAGTGCAGGACGTAAAG
AGAAAGTTAAGAAATGCAAACGTCGAGGTGCGGTTATGA

Upstream 100 bases:

>100_bases
TGAAAAAGTTAAATGATATGAACATATACGGCGTCATAATTGGTAAGGCTCTTTACGAGAAAACGATTGATTTAGAAGAA
GTGCTAGAGGTAACAAAGTT

Downstream 100 bases:

>100_bases
AACCTAACTTTTCAAAAGGACTATTACCGGCAGTTGTAATTGAAGAAGGTACAAAAGAAGTTTTAATGCTAGCTTATATG
AATGAAGAAGCATATGAAAA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MLAKRIIPCLDVKEGRVVKGVNFIGLQDVGDPVEIAALYNDAGADEIVFLDITATHEGRKTIIDVVKKTASKVFIPLTVG
GGISSVKDMYKLLRAGADKVSINSAAVRNPKLIEEGAQHFGSQCIVVAIDARKVAEGKWNVYVNGGRVDTGMDAIEWAKR
VVMLGAGEILLTSMDADGTKNGYDLRLTEEISKSVSVPVIASGGCGHADHIIEVFQKTTVDAALAASIFHYGEATVQDVK
RKLRNANVEVRL

Sequences:

>Translated_252_residues
MLAKRIIPCLDVKEGRVVKGVNFIGLQDVGDPVEIAALYNDAGADEIVFLDITATHEGRKTIIDVVKKTASKVFIPLTVG
GGISSVKDMYKLLRAGADKVSINSAAVRNPKLIEEGAQHFGSQCIVVAIDARKVAEGKWNVYVNGGRVDTGMDAIEWAKR
VVMLGAGEILLTSMDADGTKNGYDLRLTEEISKSVSVPVIASGGCGHADHIIEVFQKTTVDAALAASIFHYGEATVQDVK
RKLRNANVEVRL
>Mature_252_residues
MLAKRIIPCLDVKEGRVVKGVNFIGLQDVGDPVEIAALYNDAGADEIVFLDITATHEGRKTIIDVVKKTASKVFIPLTVG
GGISSVKDMYKLLRAGADKVSINSAAVRNPKLIEEGAQHFGSQCIVVAIDARKVAEGKWNVYVNGGRVDTGMDAIEWAKR
VVMLGAGEILLTSMDADGTKNGYDLRLTEEISKSVSVPVIASGGCGHADHIIEVFQKTTVDAALAASIFHYGEATVQDVK
RKLRNANVEVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=258, Percent_Identity=46.5116279069767, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI87082028, Length=245, Percent_Identity=28.5714285714286, Blast_Score=93, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=34.7133757961783, Blast_Score=160, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 27002; Mature: 27002

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVKEGRVVKGVNFIGLQDVGDPVEIAALYNDAGADEIVFLDITATHEGRK
CCHHHCCCCCCCCCCCEEECEEEEEECCCCCCEEEEEEECCCCCCEEEEEEEEECCCCHH
TIIDVVKKTASKVFIPLTVGGGISSVKDMYKLLRAGADKVSINSAAVRNPKLIEEGAQHF
HHHHHHHHHHCEEEEEEEECCCHHHHHHHHHHHHCCCCEEEECCHHCCCCHHHHHHHHHH
GSQCIVVAIDARKVAEGKWNVYVNGGRVDTGMDAIEWAKRVVMLGAGEILLTSMDADGTK
CCCEEEEEEECEECCCCEEEEEEECCEECCCCHHHHHHHHHHEEECCEEEEEECCCCCCC
NGYDLRLTEEISKSVSVPVIASGGCGHADHIIEVFQKTTVDAALAASIFHYGEATVQDVK
CCCEEEEHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
RKLRNANVEVRL
HHHHCCCEEEEC
>Mature Secondary Structure
MLAKRIIPCLDVKEGRVVKGVNFIGLQDVGDPVEIAALYNDAGADEIVFLDITATHEGRK
CCHHHCCCCCCCCCCCEEECEEEEEECCCCCCEEEEEEECCCCCCEEEEEEEEECCCCHH
TIIDVVKKTASKVFIPLTVGGGISSVKDMYKLLRAGADKVSINSAAVRNPKLIEEGAQHF
HHHHHHHHHHCEEEEEEEECCCHHHHHHHHHHHHCCCCEEEECCHHCCCCHHHHHHHHHH
GSQCIVVAIDARKVAEGKWNVYVNGGRVDTGMDAIEWAKRVVMLGAGEILLTSMDADGTK
CCCEEEEEEECEECCCCEEEEEEECCEECCCCHHHHHHHHHHEEECCEEEEEECCCCCCC
NGYDLRLTEEISKSVSVPVIASGGCGHADHIIEVFQKTTVDAALAASIFHYGEATVQDVK
CCCEEEEHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
RKLRNANVEVRL
HHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA