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The map label for this gene is leuB [H]

Identifier: 118476962

GI number: 118476962

Start: 1405251

End: 1406381

Strand: Direct

Name: leuB [H]

Synonym: BALH_1256

Alternate gene names: 118476962

Gene position: 1405251-1406381 (Clockwise)

Preceding gene: 118476961

Following gene: 118476963

Centisome position: 26.73

GC content: 41.64

Gene sequence:

>1131_bases
GTGAGAGGAGCTATTCTCACCTATCAAATTAAAGTTCTGAAAATTCAGTTAAAAGGAGTGTTGAAATTGGAAAAACGTAT
CGTTTGTTTAGCTGGTGATGGTGTTGGCCCAGAAGTTATGGAAAGTGCGAAGGAAGTATTGCATATGGTAGAGAGGCTAT
ATGGACATCATTTTCATTTGCAAGATGAGCATTTTGGTGGTGTTGCTATCGATTTAACGGGGCAACCATTACCACAACGA
ACACTTGCAGCTTGTTTAGCAAGTGATGCGGTTTTACTAGGCGCAGTTGGTGGACCGAGGTGGGATGGTGCGAAAGAAAG
ACCAGAGAAAGGATTATTAGCTTTAAGAAAAGGGCTTGGTGTATTTGCGAATGTTCGCCCTGTCACGGTAGAAAGTGAGA
CGGCACATTTATCACCATTAAAAAAGGCTGATGAAATTGATTTCGTTGTCGTTCGTGAATTAACAGGTGGAATTTATTTT
TCTTATCCGAAGGAACGAACGGACGAAGTAGCAACAGATACACTTACGTATCATCGCCATGAAATTGAACGTATCGTTTC
TTACGCCTTTCAATTAGCGAGCAAGCGGAAGAAAAAAGTAACATCTATTGATAAGGCGAATGTTTTAGAATCTAGTAAAC
TATGGAGAACTGTAACAGAAGAAGTAGCACTTCGTTATCCAGATGTTGAACTAGAACATATTTTAGTAGATGCGGCCGCT
ATGGAGCTAATTCGGAATCCAGGACGATTTGATGTAATTGTAACAGAAAATTTATTTGGGGATATTTTAAGTGACGAAGC
TTCCGTATTAGCTGGATCATTAGGGATGCTTCAGTCAGCAAGTCACGCGGAAAAGGGACCTTCATTATACGAGCCTATTC
ACGGATCGGCACCGGATATTGCCGGGAAAAATAAGGCGAATCCAATTGCTATGATGCGCTCTGTTGCCATGATGCTTGGG
CAATCGTTCGGATTAACGAGAGAAGGGTGTGCAATTGAAGAAGCGATTTCAGCAGTCCTTAAATCAGGGAAATGTACAGC
AGATATCGGGGGGACTGAAACGACAACTTCATTTACGAAGGCAGTTATGCAAGAAATGGAAGAACAAGCATTGGTAGGGA
GAGGAAGATAA

Upstream 100 bases:

>100_bases
GTGTTGCACAAGACGTATTGGAAGCGTCGGCAAGAGCATATGTCCACGCAGCAGGGAAATTGAAATCTTTTATCCAGCTT
GTGAAATAAGTGTTTGATAG

Downstream 100 bases:

>100_bases
TGGGGAAAAGGTTATTAGATAAACTTTGGGAAAGACACGTAGTTACGACAAATGAAAATGGATTGGATTTATTATATATC
GATCTGCACCTTGTTCATGA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MRGAILTYQIKVLKIQLKGVLKLEKRIVCLAGDGVGPEVMESAKEVLHMVERLYGHHFHLQDEHFGGVAIDLTGQPLPQR
TLAACLASDAVLLGAVGGPRWDGAKERPEKGLLALRKGLGVFANVRPVTVESETAHLSPLKKADEIDFVVVRELTGGIYF
SYPKERTDEVATDTLTYHRHEIERIVSYAFQLASKRKKKVTSIDKANVLESSKLWRTVTEEVALRYPDVELEHILVDAAA
MELIRNPGRFDVIVTENLFGDILSDEASVLAGSLGMLQSASHAEKGPSLYEPIHGSAPDIAGKNKANPIAMMRSVAMMLG
QSFGLTREGCAIEEAISAVLKSGKCTADIGGTETTTSFTKAVMQEMEEQALVGRGR

Sequences:

>Translated_376_residues
MRGAILTYQIKVLKIQLKGVLKLEKRIVCLAGDGVGPEVMESAKEVLHMVERLYGHHFHLQDEHFGGVAIDLTGQPLPQR
TLAACLASDAVLLGAVGGPRWDGAKERPEKGLLALRKGLGVFANVRPVTVESETAHLSPLKKADEIDFVVVRELTGGIYF
SYPKERTDEVATDTLTYHRHEIERIVSYAFQLASKRKKKVTSIDKANVLESSKLWRTVTEEVALRYPDVELEHILVDAAA
MELIRNPGRFDVIVTENLFGDILSDEASVLAGSLGMLQSASHAEKGPSLYEPIHGSAPDIAGKNKANPIAMMRSVAMMLG
QSFGLTREGCAIEEAISAVLKSGKCTADIGGTETTTSFTKAVMQEMEEQALVGRGR
>Mature_376_residues
MRGAILTYQIKVLKIQLKGVLKLEKRIVCLAGDGVGPEVMESAKEVLHMVERLYGHHFHLQDEHFGGVAIDLTGQPLPQR
TLAACLASDAVLLGAVGGPRWDGAKERPEKGLLALRKGLGVFANVRPVTVESETAHLSPLKKADEIDFVVVRELTGGIYF
SYPKERTDEVATDTLTYHRHEIERIVSYAFQLASKRKKKVTSIDKANVLESSKLWRTVTEEVALRYPDVELEHILVDAAA
MELIRNPGRFDVIVTENLFGDILSDEASVLAGSLGMLQSASHAEKGPSLYEPIHGSAPDIAGKNKANPIAMMRSVAMMLG
QSFGLTREGCAIEEAISAVLKSGKCTADIGGTETTTSFTKAVMQEMEEQALVGRGR

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=350, Percent_Identity=30.8571428571429, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI28178821, Length=351, Percent_Identity=30.7692307692308, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI28178816, Length=345, Percent_Identity=31.0144927536232, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI4758582, Length=259, Percent_Identity=31.2741312741313, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI28178838, Length=230, Percent_Identity=31.304347826087, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI28178819, Length=194, Percent_Identity=34.020618556701, Blast_Score=103, Evalue=4e-22,
Organism=Escherichia coli, GI87081683, Length=333, Percent_Identity=50.7507507507508, Blast_Score=315, Evalue=3e-87,
Organism=Escherichia coli, GI1788101, Length=356, Percent_Identity=35.1123595505618, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1787381, Length=373, Percent_Identity=23.5924932975871, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71986051, Length=354, Percent_Identity=31.3559322033898, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17550882, Length=357, Percent_Identity=29.9719887955182, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI25144293, Length=354, Percent_Identity=27.683615819209, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17505779, Length=347, Percent_Identity=27.3775216138329, Blast_Score=112, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6319830, Length=358, Percent_Identity=47.7653631284916, Blast_Score=288, Evalue=8e-79,
Organism=Saccharomyces cerevisiae, GI6322097, Length=357, Percent_Identity=36.6946778711485, Blast_Score=172, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6324709, Length=347, Percent_Identity=33.4293948126801, Blast_Score=153, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6324291, Length=353, Percent_Identity=29.4617563739377, Blast_Score=131, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24643270, Length=348, Percent_Identity=31.8965517241379, Blast_Score=161, Evalue=7e-40,
Organism=Drosophila melanogaster, GI24643268, Length=348, Percent_Identity=31.8965517241379, Blast_Score=161, Evalue=7e-40,
Organism=Drosophila melanogaster, GI281362242, Length=352, Percent_Identity=30.6818181818182, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24648872, Length=352, Percent_Identity=30.6818181818182, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24661184, Length=359, Percent_Identity=27.8551532033426, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI161078633, Length=348, Percent_Identity=27.0114942528736, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24650122, Length=348, Percent_Identity=27.0114942528736, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI161078635, Length=348, Percent_Identity=27.0114942528736, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI161078637, Length=348, Percent_Identity=27.0114942528736, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI161078639, Length=345, Percent_Identity=27.2463768115942, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI20130355, Length=342, Percent_Identity=23.9766081871345, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 40945; Mature: 40945

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGAILTYQIKVLKIQLKGVLKLEKRIVCLAGDGVGPEVMESAKEVLHMVERLYGHHFHL
CCCCEEEEEEEEEEEEHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEE
QDEHFGGVAIDLTGQPLPQRTLAACLASDAVLLGAVGGPRWDGAKERPEKGLLALRKGLG
CCCCCCCEEEEECCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCC
VFANVRPVTVESETAHLSPLKKADEIDFVVVRELTGGIYFSYPKERTDEVATDTLTYHRH
CEECCCEEEECCCCCCCCCCCCCCCCCEEEEEEHHCCEEEECCCHHHHHHHHHHHHHHHH
EIERIVSYAFQLASKRKKKVTSIDKANVLESSKLWRTVTEEVALRYPDVELEHILVDAAA
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
MELIRNPGRFDVIVTENLFGDILSDEASVLAGSLGMLQSASHAEKGPSLYEPIHGSAPDI
HHHHHCCCCEEEEEECHHHHHHHCCHHHHHHHHHHHHHCCHHHHCCCCHHCCCCCCCCCC
AGKNKANPIAMMRSVAMMLGQSFGLTREGCAIEEAISAVLKSGKCTADIGGTETTTSFTK
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEECCCCCCHHHHHHH
AVMQEMEEQALVGRGR
HHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRGAILTYQIKVLKIQLKGVLKLEKRIVCLAGDGVGPEVMESAKEVLHMVERLYGHHFHL
CCCCEEEEEEEEEEEEHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEE
QDEHFGGVAIDLTGQPLPQRTLAACLASDAVLLGAVGGPRWDGAKERPEKGLLALRKGLG
CCCCCCCEEEEECCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCC
VFANVRPVTVESETAHLSPLKKADEIDFVVVRELTGGIYFSYPKERTDEVATDTLTYHRH
CEECCCEEEECCCCCCCCCCCCCCCCCEEEEEEHHCCEEEECCCHHHHHHHHHHHHHHHH
EIERIVSYAFQLASKRKKKVTSIDKANVLESSKLWRTVTEEVALRYPDVELEHILVDAAA
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
MELIRNPGRFDVIVTENLFGDILSDEASVLAGSLGMLQSASHAEKGPSLYEPIHGSAPDI
HHHHHCCCCEEEEEECHHHHHHHCCHHHHHHHHHHHHHCCHHHHCCCCHHCCCCCCCCCC
AGKNKANPIAMMRSVAMMLGQSFGLTREGCAIEEAISAVLKSGKCTADIGGTETTTSFTK
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEECCCCCCHHHHHHH
AVMQEMEEQALVGRGR
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]