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The map label for this gene is leuC [H]

Identifier: 118476963

GI number: 118476963

Start: 1406381

End: 1407775

Strand: Direct

Name: leuC [H]

Synonym: BALH_1257

Alternate gene names: 118476963

Gene position: 1406381-1407775 (Clockwise)

Preceding gene: 118476962

Following gene: 229576702

Centisome position: 26.75

GC content: 41.29

Gene sequence:

>1395_bases
ATGGGGAAAAGGTTATTAGATAAACTTTGGGAAAGACACGTAGTTACGACAAATGAAAATGGATTGGATTTATTATATAT
CGATCTGCACCTTGTTCATGAAGTAACGTCACCGCAAGCCTTTGAAGGCTTGCGGCTTACAAATCGAACGGTACGCAGAC
CAGATTTAACATTCGCAACGATGGATCATAATATTCCAACGAAAGATGTTTGGAATATTACTGATCGCATTGCGAAGCAG
CAACTAGATACACTTCGGGAAAATTGCAAACAATTTCAGGTACCATTAGCGGATATCGGAGATGAAGAGCAAGGGATCGT
TCATGTTATCGGACCAGAACTCGGGCTTACGCAGCCAGGCAAAACAATAGTTTGTGGTGATAGTCATACAGCGACGCACG
GTGCGTTTGGTGCGCTAGCATTCGGCATTGGTACGAGTGAAGTGGAACATGTATTGGCAACGCAAACGCTGTGGCAAAGA
AAACCGAAAGCGATGGGCATTGAGTTAAAAGGAAAATTACAGAAAGGTGTTTACGCAAAAGATATTATTTTGCATCTCCT
TTCAAAGTACGGTGTAGCAGTTGGAACTGGATACGTAATGGAATTTTACGGAGAGACGATTGAGTCTATGGAAATGGAAG
AGAGAATGACGCTTTGCAATATGGCAATTGAAGGAGGAGCAAAAGCAGGTATTATCGCGCCGGATGAAAAAACATTTGCT
TATGTAAAAGGGCGTAAATATGCACCGAAAGACTATGAAACTTTCGAGAAAAAATGGTCTGAATTTTATACAGATGTAGA
CGCAATGTATGATTTACATATTTTAATTGACGTTACGGATTTAGCACCGTACGTTACGTGGGGAACAAATCCGAGTATGG
GTGTTCGTATTGATGAAAGGTTGCCAGAAAAGAATGATGTAAATGACGAAAGAGCATTTTCTTATATGGGATTAAGCCCT
GGACAAAGCACGTATGATATTCCAGTTCAGCATGTCTTCATTGGATCCTGTACAAATTCCAGGCTTTCTGATTTAGAAAT
TGCTGCATCTGTTGTGAAAGGGAAAAAGGTAAAAGAAGGTGTGCGAGCACTCGTTGTGCCTGGATCGAAAAGAGTAAGGG
AAGCAGCGATGCAAAAAGGACTACATCGCATATTTGAAGAAGCAGGATTTGAATGGAGAGAACCTGGATGTTCCATGTGT
CTTGGAATGAATCCAGATCAAGTACCTGAAGGAGAACATTGTGCTTCCACTTCAAATCGAAATTTTGAAGGGAGACAAGG
AAAAGGAGCACGAACACATTTAGTTAGCCCAGCAATGGCGGCAGCAGCTGCGTTATATGGTCATTTTGTTGATACTAGAA
AGGAGAGTTATGATGGAGCCATTTCGTATTCATAA

Upstream 100 bases:

>100_bases
AATGTACAGCAGATATCGGGGGGACTGAAACGACAACTTCATTTACGAAGGCAGTTATGCAAGAAATGGAAGAACAAGCA
TTGGTAGGGAGAGGAAGATA

Downstream 100 bases:

>100_bases
AGGTACTGCTGCAGTACTGATGAATGATAACATTGATACAGATCAAATTATTCCGAAGCAATATTTAAAGAGAATTGAAA
GGACGGGATTTGGAAAATAT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MGKRLLDKLWERHVVTTNENGLDLLYIDLHLVHEVTSPQAFEGLRLTNRTVRRPDLTFATMDHNIPTKDVWNITDRIAKQ
QLDTLRENCKQFQVPLADIGDEEQGIVHVIGPELGLTQPGKTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLWQR
KPKAMGIELKGKLQKGVYAKDIILHLLSKYGVAVGTGYVMEFYGETIESMEMEERMTLCNMAIEGGAKAGIIAPDEKTFA
YVKGRKYAPKDYETFEKKWSEFYTDVDAMYDLHILIDVTDLAPYVTWGTNPSMGVRIDERLPEKNDVNDERAFSYMGLSP
GQSTYDIPVQHVFIGSCTNSRLSDLEIAASVVKGKKVKEGVRALVVPGSKRVREAAMQKGLHRIFEEAGFEWREPGCSMC
LGMNPDQVPEGEHCASTSNRNFEGRQGKGARTHLVSPAMAAAAALYGHFVDTRKESYDGAISYS

Sequences:

>Translated_464_residues
MGKRLLDKLWERHVVTTNENGLDLLYIDLHLVHEVTSPQAFEGLRLTNRTVRRPDLTFATMDHNIPTKDVWNITDRIAKQ
QLDTLRENCKQFQVPLADIGDEEQGIVHVIGPELGLTQPGKTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLWQR
KPKAMGIELKGKLQKGVYAKDIILHLLSKYGVAVGTGYVMEFYGETIESMEMEERMTLCNMAIEGGAKAGIIAPDEKTFA
YVKGRKYAPKDYETFEKKWSEFYTDVDAMYDLHILIDVTDLAPYVTWGTNPSMGVRIDERLPEKNDVNDERAFSYMGLSP
GQSTYDIPVQHVFIGSCTNSRLSDLEIAASVVKGKKVKEGVRALVVPGSKRVREAAMQKGLHRIFEEAGFEWREPGCSMC
LGMNPDQVPEGEHCASTSNRNFEGRQGKGARTHLVSPAMAAAAALYGHFVDTRKESYDGAISYS
>Mature_463_residues
GKRLLDKLWERHVVTTNENGLDLLYIDLHLVHEVTSPQAFEGLRLTNRTVRRPDLTFATMDHNIPTKDVWNITDRIAKQQ
LDTLRENCKQFQVPLADIGDEEQGIVHVIGPELGLTQPGKTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLWQRK
PKAMGIELKGKLQKGVYAKDIILHLLSKYGVAVGTGYVMEFYGETIESMEMEERMTLCNMAIEGGAKAGIIAPDEKTFAY
VKGRKYAPKDYETFEKKWSEFYTDVDAMYDLHILIDVTDLAPYVTWGTNPSMGVRIDERLPEKNDVNDERAFSYMGLSPG
QSTYDIPVQHVFIGSCTNSRLSDLEIAASVVKGKKVKEGVRALVVPGSKRVREAAMQKGLHRIFEEAGFEWREPGCSMCL
GMNPDQVPEGEHCASTSNRNFEGRQGKGARTHLVSPAMAAAAALYGHFVDTRKESYDGAISYS

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=353, Percent_Identity=25.7790368271955, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI41352693, Length=368, Percent_Identity=25.8152173913043, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1786259, Length=465, Percent_Identity=58.2795698924731, Blast_Score=524, Evalue=1e-150,
Organism=Escherichia coli, GI1787531, Length=365, Percent_Identity=28.2191780821918, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI2367097, Length=337, Percent_Identity=24.0356083086053, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25149337, Length=359, Percent_Identity=30.9192200557103, Blast_Score=131, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI32564738, Length=359, Percent_Identity=30.9192200557103, Blast_Score=131, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI25149342, Length=305, Percent_Identity=30.4918032786885, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17568399, Length=425, Percent_Identity=26.5882352941176, Blast_Score=105, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6321429, Length=472, Percent_Identity=55.9322033898305, Blast_Score=497, Evalue=1e-141,
Organism=Saccharomyces cerevisiae, GI6320440, Length=414, Percent_Identity=29.2270531400966, Blast_Score=156, Evalue=7e-39,
Organism=Saccharomyces cerevisiae, GI6322261, Length=403, Percent_Identity=28.5359801488834, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6323335, Length=355, Percent_Identity=28.7323943661972, Blast_Score=134, Evalue=4e-32,
Organism=Drosophila melanogaster, GI281365315, Length=393, Percent_Identity=27.2264631043257, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI17864292, Length=393, Percent_Identity=27.2264631043257, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI161076999, Length=384, Percent_Identity=27.34375, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI28571643, Length=391, Percent_Identity=28.3887468030691, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24645686, Length=380, Percent_Identity=27.8947368421053, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI17137564, Length=373, Percent_Identity=25.201072386059, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 51565; Mature: 51434

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKRLLDKLWERHVVTTNENGLDLLYIDLHLVHEVTSPQAFEGLRLTNRTVRRPDLTFAT
CCHHHHHHHHHCCEEEECCCCCEEEEEEEEHHHHHCCCHHHCCCHHCCCCCCCCCCEEEE
MDHNIPTKDVWNITDRIAKQQLDTLRENCKQFQVPLADIGDEEQGIVHVIGPELGLTQPG
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEECCCCCCCCCC
KTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLWQRKPKAMGIELKGKLQKGVYAK
CEEEECCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEECHHHCCCHHH
DIILHLLSKYGVAVGTGYVMEFYGETIESMEMEERMTLCNMAIEGGAKAGIIAPDEKTFA
HHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCEEE
YVKGRKYAPKDYETFEKKWSEFYTDVDAMYDLHILIDVTDLAPYVTWGTNPSMGVRIDER
EEECCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCEEEECCCCCCCEEEHHH
LPEKNDVNDERAFSYMGLSPGQSTYDIPVQHVFIGSCTNSRLSDLEIAASVVKGKKVKEG
CCCCCCCCHHHHEEEECCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCHHHHHC
VRALVVPGSKRVREAAMQKGLHRIFEEAGFEWREPGCSMCLGMNPDQVPEGEHCASTSNR
CEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCHHCCCCCC
NFEGRQGKGARTHLVSPAMAAAAALYGHFVDTRKESYDGAISYS
CCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
GKRLLDKLWERHVVTTNENGLDLLYIDLHLVHEVTSPQAFEGLRLTNRTVRRPDLTFAT
CHHHHHHHHHCCEEEECCCCCEEEEEEEEHHHHHCCCHHHCCCHHCCCCCCCCCCEEEE
MDHNIPTKDVWNITDRIAKQQLDTLRENCKQFQVPLADIGDEEQGIVHVIGPELGLTQPG
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEECCCCCCCCCC
KTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLWQRKPKAMGIELKGKLQKGVYAK
CEEEECCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEECHHHCCCHHH
DIILHLLSKYGVAVGTGYVMEFYGETIESMEMEERMTLCNMAIEGGAKAGIIAPDEKTFA
HHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCEEE
YVKGRKYAPKDYETFEKKWSEFYTDVDAMYDLHILIDVTDLAPYVTWGTNPSMGVRIDER
EEECCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCEEEECCCCCCCEEEHHH
LPEKNDVNDERAFSYMGLSPGQSTYDIPVQHVFIGSCTNSRLSDLEIAASVVKGKKVKEG
CCCCCCCCHHHHEEEECCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCHHHHHC
VRALVVPGSKRVREAAMQKGLHRIFEEAGFEWREPGCSMCLGMNPDQVPEGEHCASTSNR
CEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCHHCCCCCC
NFEGRQGKGARTHLVSPAMAAAAALYGHFVDTRKESYDGAISYS
CCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA