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The map label for this gene is mesJ [H]

Identifier: 118475834

GI number: 118475834

Start: 67547

End: 68989

Strand: Direct

Name: mesJ [H]

Synonym: BALH_0061

Alternate gene names: 118475834

Gene position: 67547-68989 (Clockwise)

Preceding gene: 118475833

Following gene: 118475835

Centisome position: 1.28

GC content: 36.52

Gene sequence:

>1443_bases
GTGATTACGAAGTTGAAAGATACATTTGTTGAAAAGGTAGATGACTTTGTAAGGCAACATGATGTATTAAAGGAACGTTC
AACAATTGTTGTAGGGGTTTCTGGTGGTCCTGACTCTTTGGCTCTTCTATATTATTTATTAGAAAAAAGAGCAGCAAAAC
AGTTTGAAATTGTAGTGGCGCATGTGGATCATATGTTTAGAGGTGATGAATCTCATGAGGACCTACAGTTTGTGCAGGAC
CTTTGTAAAGGACTGGGAGTTATTTGCGAAACGATAAGGATTAATGTGTCGCAATATCAAAAGCAATACGGGATGAATGC
ACAGGTTGCTGCTAGAGAATGCAGATATGCATTTTTGGAAAGAATAATGAAGAAATATGATGCGAGATATGTAGCCCTTG
GACATCATGGTGATGATCAAGTAGAGACGATTTTAATGCGTCTTGTACGAGGGAGTACTCCGAAAGGATATGCAGGAATT
GCAGTGAAGCGTCCTTTTCATAATGGGTATTTAATTAGGCCGTTACTTGGAGTAACGAAGGAAGAAATTGTTAATTACTG
TAATGAGCTAAAAATAATTCCTCGTATAGATCCAAGTAACAAAAAGGAAGTATATACAAGGAATCGATTACGTAAATATG
TTCTTCCTCATTTGAAAGAAGAAAACCCACAAGTGCATGAGAAATTCCAAAAATTTAGCATGCAGATGCAAGAGGATGAG
GCGTATTTGCAGGAATTAGCTTTTGAGAAAATGAATAAAGTAATTACAAAAAAAAGTGATAAACAAATTAGCTTATCAAT
TCCTGCCTTTGAATCCATGTCTATGCCTTTACAAAGAAGAGGGATTCAACTAATATTAAACTATCTTTATGAATATAAGA
TTCCATCTTCTCTTTCCTCTATACATATTGACAAGGTGATTGAGTTTTTTAAGCGGACACAACCTTCAGGTTCACTTGAT
TTCCCAGGTGATTTGAAAATTGTTCGCGCGTACGAGGAGTGTAGCTTTGGATTTAAACAAGAAATTGTTTCCCCTTTCTT
ACAAGACTTATCAGTGCCCGGGACAATTACGCTATCGAACGGGGATAAACTTGTAACAGAGGTGAGCGAAGATATACCAA
GTGACATGAATGAAACAGTATTTGTTGCTAAGTATAATGATATATCATATCCAATTCGTATTCGGTCTAGAGAAAATGGA
GATCGCATGTCAATACAAGGTATGAATGGCACGAAAAAGATAAAAGCTATTTTTATCGAAGCAAAAGTACCAAGAGAAAA
AAGAGAAGAATGGCCGGTCGTTTGTGATGCAAGTGGGAATATTATTTGGTTACCCTTGTTGAAGCGATCTGCATTTGCTA
TCTCGAAAGAGACAGCAAAGAAGGATAAATATATGATTATTCACTACAAAAGCAAGGAGTCTTCCGGGAGGATAATGAAA
TGA

Upstream 100 bases:

>100_bases
AAAAATGCAATTTTTGTATATCTTTTTTTTGCACAATGTTTGTAAAAGTTGTACCATTATAAACAAGGTAACTAACGTTG
CTGTTCTTGTTTAATGAAAG

Downstream 100 bases:

>100_bases
TGAATCAAGATATCGAAAAAGTATTAATTTCTGAAGAGCAAATACAAGAAAAGGTGCTCGAATTAGGTGCGATTATTGCA
GAGGATTATAAAAACACAGT

Product: cell cycle protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MITKLKDTFVEKVDDFVRQHDVLKERSTIVVGVSGGPDSLALLYYLLEKRAAKQFEIVVAHVDHMFRGDESHEDLQFVQD
LCKGLGVICETIRINVSQYQKQYGMNAQVAARECRYAFLERIMKKYDARYVALGHHGDDQVETILMRLVRGSTPKGYAGI
AVKRPFHNGYLIRPLLGVTKEEIVNYCNELKIIPRIDPSNKKEVYTRNRLRKYVLPHLKEENPQVHEKFQKFSMQMQEDE
AYLQELAFEKMNKVITKKSDKQISLSIPAFESMSMPLQRRGIQLILNYLYEYKIPSSLSSIHIDKVIEFFKRTQPSGSLD
FPGDLKIVRAYEECSFGFKQEIVSPFLQDLSVPGTITLSNGDKLVTEVSEDIPSDMNETVFVAKYNDISYPIRIRSRENG
DRMSIQGMNGTKKIKAIFIEAKVPREKREEWPVVCDASGNIIWLPLLKRSAFAISKETAKKDKYMIIHYKSKESSGRIMK

Sequences:

>Translated_480_residues
MITKLKDTFVEKVDDFVRQHDVLKERSTIVVGVSGGPDSLALLYYLLEKRAAKQFEIVVAHVDHMFRGDESHEDLQFVQD
LCKGLGVICETIRINVSQYQKQYGMNAQVAARECRYAFLERIMKKYDARYVALGHHGDDQVETILMRLVRGSTPKGYAGI
AVKRPFHNGYLIRPLLGVTKEEIVNYCNELKIIPRIDPSNKKEVYTRNRLRKYVLPHLKEENPQVHEKFQKFSMQMQEDE
AYLQELAFEKMNKVITKKSDKQISLSIPAFESMSMPLQRRGIQLILNYLYEYKIPSSLSSIHIDKVIEFFKRTQPSGSLD
FPGDLKIVRAYEECSFGFKQEIVSPFLQDLSVPGTITLSNGDKLVTEVSEDIPSDMNETVFVAKYNDISYPIRIRSRENG
DRMSIQGMNGTKKIKAIFIEAKVPREKREEWPVVCDASGNIIWLPLLKRSAFAISKETAKKDKYMIIHYKSKESSGRIMK
>Mature_480_residues
MITKLKDTFVEKVDDFVRQHDVLKERSTIVVGVSGGPDSLALLYYLLEKRAAKQFEIVVAHVDHMFRGDESHEDLQFVQD
LCKGLGVICETIRINVSQYQKQYGMNAQVAARECRYAFLERIMKKYDARYVALGHHGDDQVETILMRLVRGSTPKGYAGI
AVKRPFHNGYLIRPLLGVTKEEIVNYCNELKIIPRIDPSNKKEVYTRNRLRKYVLPHLKEENPQVHEKFQKFSMQMQEDE
AYLQELAFEKMNKVITKKSDKQISLSIPAFESMSMPLQRRGIQLILNYLYEYKIPSSLSSIHIDKVIEFFKRTQPSGSLD
FPGDLKIVRAYEECSFGFKQEIVSPFLQDLSVPGTITLSNGDKLVTEVSEDIPSDMNETVFVAKYNDISYPIRIRSRENG
DRMSIQGMNGTKKIKAIFIEAKVPREKREEWPVVCDASGNIIWLPLLKRSAFAISKETAKKDKYMIIHYKSKESSGRIMK

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=421, Percent_Identity=27.3159144893112, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 55318; Mature: 55318

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITKLKDTFVEKVDDFVRQHDVLKERSTIVVGVSGGPDSLALLYYLLEKRAAKQFEIVVA
CCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
HVDHMFRGDESHEDLQFVQDLCKGLGVICETIRINVSQYQKQYGMNAQVAARECRYAFLE
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHH
RIMKKYDARYVALGHHGDDQVETILMRLVRGSTPKGYAGIAVKRPFHNGYLIRPLLGVTK
HHHHHHCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEEHHHHCCCH
EEIVNYCNELKIIPRIDPSNKKEVYTRNRLRKYVLPHLKEENPQVHEKFQKFSMQMQEDE
HHHHHHHHHCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
AYLQELAFEKMNKVITKKSDKQISLSIPAFESMSMPLQRRGIQLILNYLYEYKIPSSLSS
HHHHHHHHHHHHHHHHCCCCCEEEEECCCHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHH
IHIDKVIEFFKRTQPSGSLDFPGDLKIVRAYEECSFGFKQEIVSPFLQDLSVPGTITLSN
HHHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHCCCCCEEEECC
GDKLVTEVSEDIPSDMNETVFVAKYNDISYPIRIRSRENGDRMSIQGMNGTKKIKAIFIE
CCHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEEECCCCCCEEEEECCCCCCEEEEEEEE
AKVPREKREEWPVVCDASGNIIWLPLLKRSAFAISKETAKKDKYMIIHYKSKESSGRIMK
ECCCHHHHCCCCEEEECCCCEEEEEEHHCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MITKLKDTFVEKVDDFVRQHDVLKERSTIVVGVSGGPDSLALLYYLLEKRAAKQFEIVVA
CCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
HVDHMFRGDESHEDLQFVQDLCKGLGVICETIRINVSQYQKQYGMNAQVAARECRYAFLE
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHH
RIMKKYDARYVALGHHGDDQVETILMRLVRGSTPKGYAGIAVKRPFHNGYLIRPLLGVTK
HHHHHHCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEEHHHHCCCH
EEIVNYCNELKIIPRIDPSNKKEVYTRNRLRKYVLPHLKEENPQVHEKFQKFSMQMQEDE
HHHHHHHHHCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
AYLQELAFEKMNKVITKKSDKQISLSIPAFESMSMPLQRRGIQLILNYLYEYKIPSSLSS
HHHHHHHHHHHHHHHHCCCCCEEEEECCCHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHH
IHIDKVIEFFKRTQPSGSLDFPGDLKIVRAYEECSFGFKQEIVSPFLQDLSVPGTITLSN
HHHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHCCCCCEEEECC
GDKLVTEVSEDIPSDMNETVFVAKYNDISYPIRIRSRENGDRMSIQGMNGTKKIKAIFIE
CCHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEEECCCCCCEEEEECCCCCCEEEEEEEE
AKVPREKREEWPVVCDASGNIIWLPLLKRSAFAISKETAKKDKYMIIHYKSKESSGRIMK
ECCCHHHHCCCCEEEECCCCEEEEEEHHCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]