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The map label for this gene is metS [H]

Identifier: 118475810

GI number: 118475810

Start: 41580

End: 43589

Strand: Direct

Name: metS [H]

Synonym: BALH_0034

Alternate gene names: 118475810

Gene position: 41580-43589 (Clockwise)

Preceding gene: 118475808

Following gene: 118475811

Centisome position: 0.79

GC content: 36.12

Gene sequence:

>2010_bases
ATGAGGTTATCCGGGAGGTCCAAAACAATGACAGAGGAAAACAAATCCTTTTATATTACTACCCCAATTTATTATCCAAG
TGGAAAATTACACATTGGACATGCTTATACAACAGTAGCAGGAGATGCAATGGCACGATATAAGCGTATGCAAGGATATA
ATGTTCATTATTTAACAGGAACTGATGAGCATGGACAGAAAATCCAAAAGAAAGCAGAAGAATTAAACGTTACACCACAA
GCATATGTGGATAACATCGTTGCAGGAATTAAAGAACTTTGGGAAAAGATGGATATCTCTTATGATGATTTTATTCGTAC
AACGGAAGATCGTCATAAAGATGTTGTTGAAAAAATCTTCAAGCAACTAGTAGATCAAGGTGATATTTATCTTGATGAAT
ATGAAGGTTGGTATTCTGTACAAGATGAGACATTCTATACGGAGCATCAATTAGTAGATCCAATTATGGAAGGTGACAAA
GTAGTTGGAGGAAAAAGTCCAGATAGTGGTCATGATGTAGAACTTGTTCGTGAAGAATCGTATTTCTTTAGAATGGGTAA
ATATGTCGACAGACTATTAAAATTCTATGAAGATAATCCACATTTCATTCAGCCAGAGTCTCGTAAGAATGAAATGATTA
ATAACTTCATTAAACCAGGCTTAGAAGATTTAGCAGTTTCTCGTACTTCATTTGACTGGGGAGTTCGTGTTCCAGGTAAT
CCGAAACACGTTATTTACGTATGGGTTGACGCACTATCTAACTACATTACAGCATTAGGTTATGGAACAGAAAATGAAGA
GATGTATAAGAAGTTCTGGCCGGCAGATGTGCATTTAGTTGGAAAAGAAATCGTTCGTTTCCATACAATTTATTGGCCAA
TCATTTTAATGGCATTAGATTTACCTCTTCCGAAAAAAGTCTTTGCTCACGGTTGGATTTTAATGAAGGATGGAAAGATG
AGTAAGTCAAAAGGAAATGTAGTAGATCCAGTTACATTAATCGATCGTTACGGATTAGATGCATTACGTTATTACTTACT
TCGTGAAGTTCCATTCGGATCTGATGGCGTATTCACACCAGAAGGATTTGTGGAGCGTATTAATTTCGATTTAGCAAATG
ACTTAGGTAACTTATTAAATCGTACAGTAGCTATGATTGATAAGTACTTTAACGGAGAAATCCCTGCATTTAAAGCAAAT
GTAACGGAATTTGATGAAACGTTAGTAACATTTGCGAAAGATACATTGAAAAAAGTTGAAGAAGCAATGGAGAATATGGA
ATTCTCTGTAGCTCTAAGCTCAATTTGGCAATTGGTTAGCCGTACGAATAAATATATTGATGAAACACAACCATGGGTAT
TAGCGAAAGATGAGAATGATCGTGAAAAGTTGGCGTCTGTAATGGCTCATTTAGCAGAAGTACTTCGTCAAACGGGTATT
ATGCTTATGCCATTCTTAACAGTAGCACCAAGCAAAATGTTTGCTCAGCTTGGCCTTACTGATGAAGCACATACATCTTG
GGAAAGCCTATCTACAATTGGCTGTATTCCAGCTGGAACAAAAGTAGAAAAAGGACAACCAATTTTCCCTCGTTTAGAAA
TGGATGTGGAAGTGGCGTACATTAAAGAACAAATGAAAGCCTCTGCTCCTAAAGTAGAAGAGAAAAAAGAAGAAGAACCG
AAGGCAGAAGAAATTACAATTGATGATTTCTTTAAAGTAGAATTACGCGTAGCTGAAGTACTATCTGCTGAACCTGTGAA
AAAAGCGGATAAGTTGTTAAAAATTCAACTAGACTTAGGCACAGAAAAGCGTCAAGTTGTTTCTGGAATTGCAAAATTCT
ATTCGCCAGAAGACCTAAAAGGTAAAAAGGTTATTTGTGTAACAAACTTAAAACCTGTAAAATTACGCGGTGAATTATCG
CAAGGTATGATTTTAGCGGGTGAAGAGAATGGCGTATTGTCATTAGCATCTATTGACCAAAACCTACCAAATGGTACAAA
AATCAAGTAA

Upstream 100 bases:

>100_bases
AGTGGGAGTCGGGTTAATAGTCCGCGGGAGGGAACAAAACCCCGACAGATTAAAGTTTCACTTTATATATAAGTTTTTGA
TATACTATTTATAAATACAT

Downstream 100 bases:

>100_bases
TTAAGACAAGAAAAGAGATGTTTCACGTGTAACATGTGTGAAGCGTCTTTTTTCTTTTTACACTCTCATTTTTGCATTAA
GATTGTAGTATTGTTATCGT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase 1; MetRS 1 [H]

Number of amino acids: Translated: 669; Mature: 669

Protein sequence:

>669_residues
MRLSGRSKTMTEENKSFYITTPIYYPSGKLHIGHAYTTVAGDAMARYKRMQGYNVHYLTGTDEHGQKIQKKAEELNVTPQ
AYVDNIVAGIKELWEKMDISYDDFIRTTEDRHKDVVEKIFKQLVDQGDIYLDEYEGWYSVQDETFYTEHQLVDPIMEGDK
VVGGKSPDSGHDVELVREESYFFRMGKYVDRLLKFYEDNPHFIQPESRKNEMINNFIKPGLEDLAVSRTSFDWGVRVPGN
PKHVIYVWVDALSNYITALGYGTENEEMYKKFWPADVHLVGKEIVRFHTIYWPIILMALDLPLPKKVFAHGWILMKDGKM
SKSKGNVVDPVTLIDRYGLDALRYYLLREVPFGSDGVFTPEGFVERINFDLANDLGNLLNRTVAMIDKYFNGEIPAFKAN
VTEFDETLVTFAKDTLKKVEEAMENMEFSVALSSIWQLVSRTNKYIDETQPWVLAKDENDREKLASVMAHLAEVLRQTGI
MLMPFLTVAPSKMFAQLGLTDEAHTSWESLSTIGCIPAGTKVEKGQPIFPRLEMDVEVAYIKEQMKASAPKVEEKKEEEP
KAEEITIDDFFKVELRVAEVLSAEPVKKADKLLKIQLDLGTEKRQVVSGIAKFYSPEDLKGKKVICVTNLKPVKLRGELS
QGMILAGEENGVLSLASIDQNLPNGTKIK

Sequences:

>Translated_669_residues
MRLSGRSKTMTEENKSFYITTPIYYPSGKLHIGHAYTTVAGDAMARYKRMQGYNVHYLTGTDEHGQKIQKKAEELNVTPQ
AYVDNIVAGIKELWEKMDISYDDFIRTTEDRHKDVVEKIFKQLVDQGDIYLDEYEGWYSVQDETFYTEHQLVDPIMEGDK
VVGGKSPDSGHDVELVREESYFFRMGKYVDRLLKFYEDNPHFIQPESRKNEMINNFIKPGLEDLAVSRTSFDWGVRVPGN
PKHVIYVWVDALSNYITALGYGTENEEMYKKFWPADVHLVGKEIVRFHTIYWPIILMALDLPLPKKVFAHGWILMKDGKM
SKSKGNVVDPVTLIDRYGLDALRYYLLREVPFGSDGVFTPEGFVERINFDLANDLGNLLNRTVAMIDKYFNGEIPAFKAN
VTEFDETLVTFAKDTLKKVEEAMENMEFSVALSSIWQLVSRTNKYIDETQPWVLAKDENDREKLASVMAHLAEVLRQTGI
MLMPFLTVAPSKMFAQLGLTDEAHTSWESLSTIGCIPAGTKVEKGQPIFPRLEMDVEVAYIKEQMKASAPKVEEKKEEEP
KAEEITIDDFFKVELRVAEVLSAEPVKKADKLLKIQLDLGTEKRQVVSGIAKFYSPEDLKGKKVICVTNLKPVKLRGELS
QGMILAGEENGVLSLASIDQNLPNGTKIK
>Mature_669_residues
MRLSGRSKTMTEENKSFYITTPIYYPSGKLHIGHAYTTVAGDAMARYKRMQGYNVHYLTGTDEHGQKIQKKAEELNVTPQ
AYVDNIVAGIKELWEKMDISYDDFIRTTEDRHKDVVEKIFKQLVDQGDIYLDEYEGWYSVQDETFYTEHQLVDPIMEGDK
VVGGKSPDSGHDVELVREESYFFRMGKYVDRLLKFYEDNPHFIQPESRKNEMINNFIKPGLEDLAVSRTSFDWGVRVPGN
PKHVIYVWVDALSNYITALGYGTENEEMYKKFWPADVHLVGKEIVRFHTIYWPIILMALDLPLPKKVFAHGWILMKDGKM
SKSKGNVVDPVTLIDRYGLDALRYYLLREVPFGSDGVFTPEGFVERINFDLANDLGNLLNRTVAMIDKYFNGEIPAFKAN
VTEFDETLVTFAKDTLKKVEEAMENMEFSVALSSIWQLVSRTNKYIDETQPWVLAKDENDREKLASVMAHLAEVLRQTGI
MLMPFLTVAPSKMFAQLGLTDEAHTSWESLSTIGCIPAGTKVEKGQPIFPRLEMDVEVAYIKEQMKASAPKVEEKKEEEP
KAEEITIDDFFKVELRVAEVLSAEPVKKADKLLKIQLDLGTEKRQVVSGIAKFYSPEDLKGKKVICVTNLKPVKLRGELS
QGMILAGEENGVLSLASIDQNLPNGTKIK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=505, Percent_Identity=35.2475247524752, Blast_Score=308, Evalue=1e-83,
Organism=Homo sapiens, GI14043022, Length=656, Percent_Identity=25.9146341463415, Blast_Score=182, Evalue=1e-45,
Organism=Escherichia coli, GI1788432, Length=704, Percent_Identity=29.8295454545455, Blast_Score=292, Evalue=6e-80,
Organism=Escherichia coli, GI1789455, Length=108, Percent_Identity=34.2592592592593, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1786861, Length=211, Percent_Identity=26.0663507109005, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI212645096, Length=491, Percent_Identity=32.5865580448065, Blast_Score=275, Evalue=5e-74,
Organism=Caenorhabditis elegans, GI71992781, Length=383, Percent_Identity=31.8537859007833, Blast_Score=196, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI17540794, Length=593, Percent_Identity=25.1264755480607, Blast_Score=165, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI71997510, Length=181, Percent_Identity=29.2817679558011, Blast_Score=66, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI71997517, Length=181, Percent_Identity=29.2817679558011, Blast_Score=65, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6321610, Length=523, Percent_Identity=31.3575525812619, Blast_Score=260, Evalue=5e-70,
Organism=Saccharomyces cerevisiae, GI6321704, Length=484, Percent_Identity=24.7933884297521, Blast_Score=123, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6323414, Length=237, Percent_Identity=28.2700421940928, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI21358483, Length=574, Percent_Identity=32.7526132404181, Blast_Score=293, Evalue=4e-79,
Organism=Drosophila melanogaster, GI24655311, Length=562, Percent_Identity=26.8683274021352, Blast_Score=178, Evalue=9e-45,
Organism=Drosophila melanogaster, GI21355409, Length=199, Percent_Identity=27.6381909547739, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 76255; Mature: 76255

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSGRSKTMTEENKSFYITTPIYYPSGKLHIGHAYTTVAGDAMARYKRMQGYNVHYLTG
CCCCCCCCCCCCCCCEEEEEECEEECCCCEEECCCHHHHHHHHHHHHHHHCCCEEEEECC
TDEHGQKIQKKAEELNVTPQAYVDNIVAGIKELWEKMDISYDDFIRTTEDRHKDVVEKIF
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHH
KQLVDQGDIYLDEYEGWYSVQDETFYTEHQLVDPIMEGDKVVGGKSPDSGHDVELVREES
HHHHCCCCEEEECCCCEEECCCCEEEHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEHHH
YFFRMGKYVDRLLKFYEDNPHFIQPESRKNEMINNFIKPGLEDLAVSRTSFDWGVRVPGN
HHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCEECCCC
PKHVIYVWVDALSNYITALGYGTENEEMYKKFWPADVHLVGKEIVRFHTIYWPIILMALD
CCEEEEEEHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHC
LPLPKKVFAHGWILMKDGKMSKSKGNVVDPVTLIDRYGLDALRYYLLREVPFGSDGVFTP
CCCCHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCC
EGFVERINFDLANDLGNLLNRTVAMIDKYFNGEIPAFKANVTEFDETLVTFAKDTLKKVE
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHH
EAMENMEFSVALSSIWQLVSRTNKYIDETQPWVLAKDENDREKLASVMAHLAEVLRQTGI
HHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCC
MLMPFLTVAPSKMFAQLGLTDEAHTSWESLSTIGCIPAGTKVEKGQPIFPRLEMDVEVAY
EEEHHHHHCHHHHHHHHCCCCHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHH
IKEQMKASAPKVEEKKEEEPKAEEITIDDFFKVELRVAEVLSAEPVKKADKLLKIQLDLG
HHHHHHHCCCCCHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCCHHHHCCEEEEEECCC
TEKRQVVSGIAKFYSPEDLKGKKVICVTNLKPVKLRGELSQGMILAGEENGVLSLASIDQ
CHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEECCCCCCEEEECCCCCEEEEEECCC
NLPNGTKIK
CCCCCCCCC
>Mature Secondary Structure
MRLSGRSKTMTEENKSFYITTPIYYPSGKLHIGHAYTTVAGDAMARYKRMQGYNVHYLTG
CCCCCCCCCCCCCCCEEEEEECEEECCCCEEECCCHHHHHHHHHHHHHHHCCCEEEEECC
TDEHGQKIQKKAEELNVTPQAYVDNIVAGIKELWEKMDISYDDFIRTTEDRHKDVVEKIF
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHH
KQLVDQGDIYLDEYEGWYSVQDETFYTEHQLVDPIMEGDKVVGGKSPDSGHDVELVREES
HHHHCCCCEEEECCCCEEECCCCEEEHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEHHH
YFFRMGKYVDRLLKFYEDNPHFIQPESRKNEMINNFIKPGLEDLAVSRTSFDWGVRVPGN
HHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCEECCCC
PKHVIYVWVDALSNYITALGYGTENEEMYKKFWPADVHLVGKEIVRFHTIYWPIILMALD
CCEEEEEEHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHC
LPLPKKVFAHGWILMKDGKMSKSKGNVVDPVTLIDRYGLDALRYYLLREVPFGSDGVFTP
CCCCHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCC
EGFVERINFDLANDLGNLLNRTVAMIDKYFNGEIPAFKANVTEFDETLVTFAKDTLKKVE
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHH
EAMENMEFSVALSSIWQLVSRTNKYIDETQPWVLAKDENDREKLASVMAHLAEVLRQTGI
HHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCC
MLMPFLTVAPSKMFAQLGLTDEAHTSWESLSTIGCIPAGTKVEKGQPIFPRLEMDVEVAY
EEEHHHHHCHHHHHHHHCCCCHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHH
IKEQMKASAPKVEEKKEEEPKAEEITIDDFFKVELRVAEVLSAEPVKKADKLLKIQLDLG
HHHHHHHCCCCCHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCCHHHHCCEEEEEECCC
TEKRQVVSGIAKFYSPEDLKGKKVICVTNLKPVKLRGELSQGMILAGEENGVLSLASIDQ
CHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEECCCCCCEEEECCCCCEEEEEECCC
NLPNGTKIK
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]