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The map label for this gene is dnaN [H]

Identifier: 118475780

GI number: 118475780

Start: 1927

End: 3066

Strand: Direct

Name: dnaN [H]

Synonym: BALH_0002

Alternate gene names: 118475780

Gene position: 1927-3066 (Clockwise)

Preceding gene: 118475779

Following gene: 118475781

Centisome position: 0.04

GC content: 32.46

Gene sequence:

>1140_bases
ATGCGTTTTTCAATTCAAAAAGACTATCTTGTAAGAAGTGTACAAGATGTAATGAAGGCCGTTTCTTCTCGTACAACAAT
TCCGATCCTTACAGGAATTAAAGTTGTCGCTACGGAAGAAGGAGTTACATTAACAGGAAGCGATGCTGATATTTCGATTG
AATCTTTTATCCCAGTTGAAGAGGATGGAAAAGAAATTGTAGAAGTAAAACAATCAGGAAGTATTGTTTTACAGGCTAAA
TATTTTAGTGAAATTGTAAAAAAATTGCCGAAAGAAACTGTAGAAATTTCTGTCGAAAATCATTTAATGACAAAAATAAC
TTCTGGGAAATCAGAATTTAATTTAAATGGTTTAGATTCTGCAGAATATCCATTGTTACCACAAATTGAAGAACATCATG
TTTTTAAGATTCCAACAGATTTACTTAAACATATGATCAGACAAACTGTATTTGCAGTCTCCACTTCTGAAACAAGACCG
ATCTTGACAGGTGTAAACTGGAAGGTATATAACAGCGAACTAACTTGTATTGCTACAGATAGTCACAGGTTAGCTCTTCG
AAAAGCAAAAATTGAAGGTATTGTAGATGAATTCCAGGCAAATGTTGTTATTCCGGGGAAAAGCTTAAATGAATTAAGCA
AAATTCTAGATGAGTCTGAAGAAATGGTAGATATCGTTATTACGGAGTATCAAGTATTATTCCGTACAAAACATTTATTA
TTCTTCTCAAGATTGTTAGAAGGAAATTATCCTGATACAACTCGATTAATTCCTGCAGAGAGTAAAACAGATATTTTTGT
AAATACAAAAGAATTTTTACAAGCAATTGATCGTGCATCTCTGTTAGCTAGAGATGGTCGTAATAATGTTGTGAAATTAT
CAACTTTAGAGCAGGCAATGCTAGAAATTTCTTCGAATTCACCAGAAATCGGGAAAGTAGTAGAAGAAGTTCAATGTGAA
AAAGTAGATGGAGAAGAGTTAAAAATATCTTTTAGTGCAAAATATATGATGGATGCACTAAAGGCATTAGATAGTACTGA
AATTAAGATTAGCTTTACTGGAGCAATGAGACCATTCTTAATTCGTACGGTAAATGATGAATCCATTATTCAATTAATTT
TACCGGTTCGTACTTACTAA

Upstream 100 bases:

>100_bases
AACGGGGTTATCCACATATCCACAAGCCCTATTACTATTACTACTATTTTTTATCTTTATTAATTAAATAAAATCTTATA
CTTACCGGAGGTTTTTCTTT

Downstream 100 bases:

>100_bases
GTAAGAAATAAGGGTTGCTAGTTTTCAGATGCTAGTAGCCCTTATTTGATTTTTGGGTATTACTTTCCTAATGCTAGTTT
ATTTAGTACAATGAAAGAAT

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MRFSIQKDYLVRSVQDVMKAVSSRTTIPILTGIKVVATEEGVTLTGSDADISIESFIPVEEDGKEIVEVKQSGSIVLQAK
YFSEIVKKLPKETVEISVENHLMTKITSGKSEFNLNGLDSAEYPLLPQIEEHHVFKIPTDLLKHMIRQTVFAVSTSETRP
ILTGVNWKVYNSELTCIATDSHRLALRKAKIEGIVDEFQANVVIPGKSLNELSKILDESEEMVDIVITEYQVLFRTKHLL
FFSRLLEGNYPDTTRLIPAESKTDIFVNTKEFLQAIDRASLLARDGRNNVVKLSTLEQAMLEISSNSPEIGKVVEEVQCE
KVDGEELKISFSAKYMMDALKALDSTEIKISFTGAMRPFLIRTVNDESIIQLILPVRTY

Sequences:

>Translated_379_residues
MRFSIQKDYLVRSVQDVMKAVSSRTTIPILTGIKVVATEEGVTLTGSDADISIESFIPVEEDGKEIVEVKQSGSIVLQAK
YFSEIVKKLPKETVEISVENHLMTKITSGKSEFNLNGLDSAEYPLLPQIEEHHVFKIPTDLLKHMIRQTVFAVSTSETRP
ILTGVNWKVYNSELTCIATDSHRLALRKAKIEGIVDEFQANVVIPGKSLNELSKILDESEEMVDIVITEYQVLFRTKHLL
FFSRLLEGNYPDTTRLIPAESKTDIFVNTKEFLQAIDRASLLARDGRNNVVKLSTLEQAMLEISSNSPEIGKVVEEVQCE
KVDGEELKISFSAKYMMDALKALDSTEIKISFTGAMRPFLIRTVNDESIIQLILPVRTY
>Mature_379_residues
MRFSIQKDYLVRSVQDVMKAVSSRTTIPILTGIKVVATEEGVTLTGSDADISIESFIPVEEDGKEIVEVKQSGSIVLQAK
YFSEIVKKLPKETVEISVENHLMTKITSGKSEFNLNGLDSAEYPLLPQIEEHHVFKIPTDLLKHMIRQTVFAVSTSETRP
ILTGVNWKVYNSELTCIATDSHRLALRKAKIEGIVDEFQANVVIPGKSLNELSKILDESEEMVDIVITEYQVLFRTKHLL
FFSRLLEGNYPDTTRLIPAESKTDIFVNTKEFLQAIDRASLLARDGRNNVVKLSTLEQAMLEISSNSPEIGKVVEEVQCE
KVDGEELKISFSAKYMMDALKALDSTEIKISFTGAMRPFLIRTVNDESIIQLILPVRTY

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=379, Percent_Identity=26.3852242744063, Blast_Score=149, Evalue=2e-37,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 42591; Mature: 42591

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFSIQKDYLVRSVQDVMKAVSSRTTIPILTGIKVVATEEGVTLTGSDADISIESFIPVE
CCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECEEEEEECCCEEEECCCCCEEHHHCCCCC
EDGKEIVEVKQSGSIVLQAKYFSEIVKKLPKETVEISVENHLMTKITSGKSEFNLNGLDS
CCCHHHHHHHCCCCEEEEEHHHHHHHHHCCCHHEEEEECCCEEEEEECCCCCCCCCCCCC
AEYPLLPQIEEHHVFKIPTDLLKHMIRQTVFAVSTSETRPILTGVNWKVYNSELTCIATD
CCCCCCCCCCCCCEEECCHHHHHHHHHHHHHEEECCCCCCEEECCEEEEECCEEEEEEEC
SHRLALRKAKIEGIVDEFQANVVIPGKSLNELSKILDESEEMVDIVITEYQVLFRTKHLL
CCHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
FFSRLLEGNYPDTTRLIPAESKTDIFVNTKEFLQAIDRASLLARDGRNNVVKLSTLEQAM
HHHHHHCCCCCCCCEEECCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHH
LEISSNSPEIGKVVEEVQCEKVDGEELKISFSAKYMMDALKALDSTEIKISFTGAMRPFL
HHHCCCCCHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHCCCCEEEEEEECCCCCEE
IRTVNDESIIQLILPVRTY
EEECCCHHHEEEEECCCCC
>Mature Secondary Structure
MRFSIQKDYLVRSVQDVMKAVSSRTTIPILTGIKVVATEEGVTLTGSDADISIESFIPVE
CCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECEEEEEECCCEEEECCCCCEEHHHCCCCC
EDGKEIVEVKQSGSIVLQAKYFSEIVKKLPKETVEISVENHLMTKITSGKSEFNLNGLDS
CCCHHHHHHHCCCCEEEEEHHHHHHHHHCCCHHEEEEECCCEEEEEECCCCCCCCCCCCC
AEYPLLPQIEEHHVFKIPTDLLKHMIRQTVFAVSTSETRPILTGVNWKVYNSELTCIATD
CCCCCCCCCCCCCEEECCHHHHHHHHHHHHHEEECCCCCCEEECCEEEEECCEEEEEEEC
SHRLALRKAKIEGIVDEFQANVVIPGKSLNELSKILDESEEMVDIVITEYQVLFRTKHLL
CCHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
FFSRLLEGNYPDTTRLIPAESKTDIFVNTKEFLQAIDRASLLARDGRNNVVKLSTLEQAM
HHHHHHCCCCCCCCEEECCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHH
LEISSNSPEIGKVVEEVQCEKVDGEELKISFSAKYMMDALKALDSTEIKISFTGAMRPFL
HHHCCCCCHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHCCCCEEEEEEECCCCCEE
IRTVNDESIIQLILPVRTY
EEECCCHHHEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987847; 7584024; 9384377; 2846289 [H]