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The map label for this gene is yafC [H]

Identifier: 117922034

GI number: 117922034

Start: 4321829

End: 4322710

Strand: Direct

Name: yafC [H]

Synonym: Shewana3_3598

Alternate gene names: 117922034

Gene position: 4321829-4322710 (Clockwise)

Preceding gene: 117922032

Following gene: 117922035

Centisome position: 86.92

GC content: 50.0

Gene sequence:

>882_bases
ATGCTGACTCGCTCAGATGACTTACAGATACTGCTCACAGTGGTCGATAGTGGCGGATTTTCCGCGGCGGCCGAAGCGCT
CGATATTCAAGTCGCGAGGGTTTCAAGAGCCGTGACTAAACTTGAGAGTCAACTACAAGTTACCCTGCTCAACCGCACCA
CTCGACGAGTCGAATTAACCAATGAGGGACGCCTGTTTGTCGATGCAGTTAGGCTTGGGCTGCAAACCATACAGTTAGCG
GAAGAAGAACTTATGAGCCGCGGGGAGCAACCTAAGGGTAAGCTTAGAGTCGATGCAGCCAGCCCTTTTGTTCTTCATCA
GATTGTCCCCCTAGTGCATGAGTTTAAACAGCTTTACCCCGAGATAGAGCTAGAACTCACCTCGAACGAAGGCTTTGTCG
ACTTGATTGAGAAGCGCACCGATGTGGCGATTCGTATCGGAAAACTCACCGATTCAACCCTGCACGCTAGACCCTTAGGT
AAGAGTAAACTGCATATTGTCGCCTCTAAGGAATACCTCGCCAAACGCGGTTTACCTAAAAGCGTCAGTGAGCTAAACCA
CCATAGCCTTATCGGTTTTACGGGCGCTAAGGTCTTGAATCAATGGCCACTCAAAGGACTGGAAACCATTGTTCCCACAA
TGCGGGCAAGCAATGGCGAAACCGTACGTCAATTAGCCCTCGCGGGTAACGGTATAGCCTGCCTATCTGGATTTATGGTC
AGCGATGATATTGCTAGCGGGCGTTTATTGCCTTTGCTGGAAAACGAGCGAGTCGCAAACATAGAGCGGGAGCAAGTCAA
TGCCGTTTATTACAAGGCCTCAACCGTAGCTCGGCGGATCTCCGCCTTTATCGATTTTATTCAACCTCGGCTAACCCTAT
AA

Upstream 100 bases:

>100_bases
AGATGGCGCTATCTTGACGATAAAGTTTATTGTCAAAAAGAGTAATTTTTGCAAATGATTTTTGCTATAAACGTAATTAA
TTACCTTGTTGGAGACTGAC

Downstream 100 bases:

>100_bases
ATTTGCAAGTGATTAGGGCGTTAAGTGCCATAGATTGTTGCAATAATAGCAAAAATCATTTGTGCTTTAGCCTCTTTATC
GACAAAGCACGATCGGCGAC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MLTRSDDLQILLTVVDSGGFSAAAEALDIQVARVSRAVTKLESQLQVTLLNRTTRRVELTNEGRLFVDAVRLGLQTIQLA
EEELMSRGEQPKGKLRVDAASPFVLHQIVPLVHEFKQLYPEIELELTSNEGFVDLIEKRTDVAIRIGKLTDSTLHARPLG
KSKLHIVASKEYLAKRGLPKSVSELNHHSLIGFTGAKVLNQWPLKGLETIVPTMRASNGETVRQLALAGNGIACLSGFMV
SDDIASGRLLPLLENERVANIEREQVNAVYYKASTVARRISAFIDFIQPRLTL

Sequences:

>Translated_293_residues
MLTRSDDLQILLTVVDSGGFSAAAEALDIQVARVSRAVTKLESQLQVTLLNRTTRRVELTNEGRLFVDAVRLGLQTIQLA
EEELMSRGEQPKGKLRVDAASPFVLHQIVPLVHEFKQLYPEIELELTSNEGFVDLIEKRTDVAIRIGKLTDSTLHARPLG
KSKLHIVASKEYLAKRGLPKSVSELNHHSLIGFTGAKVLNQWPLKGLETIVPTMRASNGETVRQLALAGNGIACLSGFMV
SDDIASGRLLPLLENERVANIEREQVNAVYYKASTVARRISAFIDFIQPRLTL
>Mature_293_residues
MLTRSDDLQILLTVVDSGGFSAAAEALDIQVARVSRAVTKLESQLQVTLLNRTTRRVELTNEGRLFVDAVRLGLQTIQLA
EEELMSRGEQPKGKLRVDAASPFVLHQIVPLVHEFKQLYPEIELELTSNEGFVDLIEKRTDVAIRIGKLTDSTLHARPLG
KSKLHIVASKEYLAKRGLPKSVSELNHHSLIGFTGAKVLNQWPLKGLETIVPTMRASNGETVRQLALAGNGIACLSGFMV
SDDIASGRLLPLLENERVANIEREQVNAVYYKASTVARRISAFIDFIQPRLTL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=291, Percent_Identity=45.360824742268, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI1789639, Length=258, Percent_Identity=32.5581395348837, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI87081978, Length=290, Percent_Identity=31.3793103448276, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=30.1369863013699, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789440, Length=294, Percent_Identity=30.952380952381, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1787128, Length=286, Percent_Identity=25.8741258741259, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787589, Length=257, Percent_Identity=26.8482490272374, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI157672245, Length=175, Percent_Identity=28, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788706, Length=173, Percent_Identity=28.9017341040462, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790399, Length=313, Percent_Identity=24.6006389776358, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788481, Length=292, Percent_Identity=22.6027397260274, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786448, Length=246, Percent_Identity=24.7967479674797, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789173, Length=248, Percent_Identity=27.4193548387097, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI87082132, Length=290, Percent_Identity=23.7931034482759, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI145693105, Length=193, Percent_Identity=26.4248704663212, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32383; Mature: 32383

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRSDDLQILLTVVDSGGFSAAAEALDIQVARVSRAVTKLESQLQVTLLNRTTRRVELT
CCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEEEEEEC
NEGRLFVDAVRLGLQTIQLAEEELMSRGEQPKGKLRVDAASPFVLHQIVPLVHEFKQLYP
CCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCC
EIELELTSNEGFVDLIEKRTDVAIRIGKLTDSTLHARPLGKSKLHIVASKEYLAKRGLPK
CEEEEEECCCCHHHHHHCCCCEEEEEECCCCCCEECCCCCCCEEEEEECHHHHHHCCCCH
SVSELNHHSLIGFTGAKVLNQWPLKGLETIVPTMRASNGETVRQLALAGNGIACLSGFMV
HHHHCCCCEEEEEHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHCEEE
SDDIASGRLLPLLENERVANIEREQVNAVYYKASTVARRISAFIDFIQPRLTL
ECCCCCCCEEEEECCCCCCCCHHHHHHHHEEHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLTRSDDLQILLTVVDSGGFSAAAEALDIQVARVSRAVTKLESQLQVTLLNRTTRRVELT
CCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEEEEEEC
NEGRLFVDAVRLGLQTIQLAEEELMSRGEQPKGKLRVDAASPFVLHQIVPLVHEFKQLYP
CCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCC
EIELELTSNEGFVDLIEKRTDVAIRIGKLTDSTLHARPLGKSKLHIVASKEYLAKRGLPK
CEEEEEECCCCHHHHHHCCCCEEEEEECCCCCCEECCCCCCCEEEEEECHHHHHHCCCCH
SVSELNHHSLIGFTGAKVLNQWPLKGLETIVPTMRASNGETVRQLALAGNGIACLSGFMV
HHHHCCCCEEEEEHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHCEEE
SDDIASGRLLPLLENERVANIEREQVNAVYYKASTVARRISAFIDFIQPRLTL
ECCCCCCCEEEEECCCCCCCCHHHHHHHHEEHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]