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The map label for this gene is phaZ1 [H]

Identifier: 117921894

GI number: 117921894

Start: 4142698

End: 4143624

Strand: Direct

Name: phaZ1 [H]

Synonym: Shewana3_3457

Alternate gene names: 117921894

Gene position: 4142698-4143624 (Clockwise)

Preceding gene: 117921890

Following gene: 117921895

Centisome position: 83.32

GC content: 49.51

Gene sequence:

>927_bases
ATGCGCAAATTGACACTCATTGCCACTTGCCTCAGCGCACTGTTATTCACCCCCGCCTATGCCCAGAGTGAGTATCAGGC
ATTTAGCGAATTTGGCGCCAATCCCGGTGAGTTAACCGCCTCCTATTTATCGCCCGAAGGTCCAGGCCAAGATGCGCTAG
TAGTGCTACTGCACGGCTGTGTGCAGGATGGCGTGGAACTGGCCAACAATAGCGGTTTAACCGCCCTCGCCCAAGAGAAA
AAGTTTAGCTTACTGGTGCCACAGCAGAGTTTTGATAACAATGTAAAGCGTTGTTATAACTGGTTTTCAGCGCAGGATAC
TCAGGTGGATAGCGGCGAGATGCTATCGCTGAAAAACATGATAGCCAAAGCCCAATCCCAATCCGGCGCAAAACGCGTGT
ATGTGATTGGCCTATCCGCCGGTGGCGCTATGGCCAGCGCCGCACTGGTTAACTATCCCGATTTATTCACCTCAGGCGCG
GTGATCGCAGGCCTGCCTTACCCCTGCGCTGACAACCTGACCAAAGCCATTTCCTGCATGAAAAAGGGCCCAGCAGAGTC
TGTTGAAGAATTAGTGAGTTTTGCCAAACAGGTGCATCCGAACCAACAACACTGGCCCGCCTTAACCGTGTGGACAGGGC
AAAATGATGTGGTGGTGAATCCTGAAAATGCCAAACAACTCTCAGCCCAGTGGGTCGCACTGACCCAAGCGAATAAAACA
CCAAGAGTCGAACAATATGCCGACTATAGTGTTAGCACTTGGTCCGATACGAGTGGCAATAACCTTATTTCGTTAGTCGA
AATAAATAATATGGGCCATGGCATTGCCGTTAATCCAGATATTAAAAATGGCGGGAACACGGGAGACTTTTTACTAAAAG
CACCTATCAGCAGCATGTCAGAAATAATCAATCTTTGGGGAATATAG

Upstream 100 bases:

>100_bases
AATGAACGCAGGGCAGCATCAAGCTCTGGAACTAAAGTGCCATTGTCAGGCGCCGCAAAATTCAGCAAGTTCTCACTATC
ATGAACTTGGAAGCACTCCT

Downstream 100 bases:

>100_bases
GGTTTAATAATAAATAAACCTTGAGTATCGATATTCAACGTATCGGTATAAAAATAAAAACAATGAGTTAAAAACACATA
CTACTATAGTACCGATTTAA

Product: PHB depolymerase family esterase

Products: NA

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MRKLTLIATCLSALLFTPAYAQSEYQAFSEFGANPGELTASYLSPEGPGQDALVVLLHGCVQDGVELANNSGLTALAQEK
KFSLLVPQQSFDNNVKRCYNWFSAQDTQVDSGEMLSLKNMIAKAQSQSGAKRVYVIGLSAGGAMASAALVNYPDLFTSGA
VIAGLPYPCADNLTKAISCMKKGPAESVEELVSFAKQVHPNQQHWPALTVWTGQNDVVVNPENAKQLSAQWVALTQANKT
PRVEQYADYSVSTWSDTSGNNLISLVEINNMGHGIAVNPDIKNGGNTGDFLLKAPISSMSEIINLWGI

Sequences:

>Translated_308_residues
MRKLTLIATCLSALLFTPAYAQSEYQAFSEFGANPGELTASYLSPEGPGQDALVVLLHGCVQDGVELANNSGLTALAQEK
KFSLLVPQQSFDNNVKRCYNWFSAQDTQVDSGEMLSLKNMIAKAQSQSGAKRVYVIGLSAGGAMASAALVNYPDLFTSGA
VIAGLPYPCADNLTKAISCMKKGPAESVEELVSFAKQVHPNQQHWPALTVWTGQNDVVVNPENAKQLSAQWVALTQANKT
PRVEQYADYSVSTWSDTSGNNLISLVEINNMGHGIAVNPDIKNGGNTGDFLLKAPISSMSEIINLWGI
>Mature_308_residues
MRKLTLIATCLSALLFTPAYAQSEYQAFSEFGANPGELTASYLSPEGPGQDALVVLLHGCVQDGVELANNSGLTALAQEK
KFSLLVPQQSFDNNVKRCYNWFSAQDTQVDSGEMLSLKNMIAKAQSQSGAKRVYVIGLSAGGAMASAALVNYPDLFTSGA
VIAGLPYPCADNLTKAISCMKKGPAESVEELVSFAKQVHPNQQHWPALTVWTGQNDVVVNPENAKQLSAQWVALTQANKT
PRVEQYADYSVSTWSDTSGNNLISLVEINNMGHGIAVNPDIKNGGNTGDFLLKAPISSMSEIINLWGI

Specific function: Specific for poly(hydroxyalkanoic acid) consisting of monomers of four or five carbon atoms and for P- nitrophenylbutyrate as substrates [H]

COG id: COG3509

COG function: function code Q; Poly(3-hydroxybutyrate) depolymerase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010126 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 32992; Mature: 32992

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLTLIATCLSALLFTPAYAQSEYQAFSEFGANPGELTASYLSPEGPGQDALVVLLHGC
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHEEHHHCCCCCCCCHHHHHHHHHH
VQDGVELANNSGLTALAQEKKFSLLVPQQSFDNNVKRCYNWFSAQDTQVDSGEMLSLKNM
HHHHHHHHCCCCCEEEECCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
IAKAQSQSGAKRVYVIGLSAGGAMASAALVNYPDLFTSGAVIAGLPYPCADNLTKAISCM
HHHHHCCCCCCEEEEEEECCCCHHHHHHHHCCCHHHHCCCEEECCCCCCHHHHHHHHHHH
KKGPAESVEELVSFAKQVHPNQQHWPALTVWTGQNDVVVNPENAKQLSAQWVALTQANKT
HCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEECCCCHHHHHHHEEEEECCCCC
PRVEQYADYSVSTWSDTSGNNLISLVEINNMGHGIAVNPDIKNGGNTGDFLLKAPISSMS
CCHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCEEECCCCCCCCCCCCEEEECCHHHHH
EIINLWGI
HHHHHHCC
>Mature Secondary Structure
MRKLTLIATCLSALLFTPAYAQSEYQAFSEFGANPGELTASYLSPEGPGQDALVVLLHGC
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHEEHHHCCCCCCCCHHHHHHHHHH
VQDGVELANNSGLTALAQEKKFSLLVPQQSFDNNVKRCYNWFSAQDTQVDSGEMLSLKNM
HHHHHHHHCCCCCEEEECCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
IAKAQSQSGAKRVYVIGLSAGGAMASAALVNYPDLFTSGAVIAGLPYPCADNLTKAISCM
HHHHHCCCCCCEEEEEEECCCCHHHHHHHHCCCHHHHCCCEEECCCCCCHHHHHHHHHHH
KKGPAESVEELVSFAKQVHPNQQHWPALTVWTGQNDVVVNPENAKQLSAQWVALTQANKT
HCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEECCCCHHHHHHHEEEEECCCCC
PRVEQYADYSVSTWSDTSGNNLISLVEINNMGHGIAVNPDIKNGGNTGDFLLKAPISSMS
CCHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCEEECCCCCCCCCCCCEEEECCHHHHH
EIINLWGI
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8269961 [H]