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The map label for this gene is gshB [H]

Identifier: 117921882

GI number: 117921882

Start: 4127283

End: 4128230

Strand: Direct

Name: gshB [H]

Synonym: Shewana3_3445

Alternate gene names: 117921882

Gene position: 4127283-4128230 (Clockwise)

Preceding gene: 117921881

Following gene: 117921883

Centisome position: 83.01

GC content: 52.43

Gene sequence:

>948_bases
ATGATAAAACTCGGCATAGTGATGGACCCCATCAGTGAGATCAACATTAAGAAAGACTCCAGTTTTGCCATGCTAATGGC
GGCGCAGGAAAGGGGTTACCAACTGTTTTATATGGAAATGGCCGACCTCGCCATGGTCAACGGTGTTGCCATGGGCAATA
TGCGTCCATTAAAGGTCATCAACGATGCCAATAAATGGTTCGAACTGGGCGACGCCCAAGACACGCCGCTGAGCGAGCTG
AACGTGGTGTTAATGCGTAAAGACCCACCGTTCGATACCGAATTTATCTACGCCACTTACATGCTAGAGCGCGCCGAAGA
GCAAGGCGTGCTGATTGTGAATAAACCGCAAAGCTTGCGCGATGCCAACGAGAAGCTATTCACCGCATGGTTTAGCGAAT
TTACGCCAGAGACCATAGTGACCCGCGATGCTAATCGCATCCGCGCGTTCCACCAAGCCAAGGGCGACATTATCCTCAAG
CCATTAGACGGCATGGGCGGCACCTCGATTTTCCGCGTGAAGCAGGACGATCCTAACCTTGGGGTGATCATCGAAACCTT
GACCCAATACGGCAACCAATACGCCATGGCGCAGGCTTTTATTCCTGAAATCACTAAGGGCGACAAGCGTATTCTGGTGG
TGGACGGTGAGCCCGTGCCCTACGCTTTAGCGCGTATTCCGAAAAAAGGCGAAACCCGCGGTAACTTGGCCGCAGGCGGC
AGCGGTGTTGCTCAGCCGTTATCGGAATCAGATTGGAAAATCGCCCGCGCCATTGGCCCAGAGCTGAAAAAACGCGGCCT
AATTTTTGTGGGTCTGGATGTGATTGGTGACAAGCTGACCGAGATTAACGTCACTAGCCCAACCTGTATCCGTGAAATCC
AAGCGGCATTCGATGTGGACATTACTGGCATGCTGTTCGATGCCATCGAAGCCCGTCTTGCCCAGTAA

Upstream 100 bases:

>100_bases
AATGGTTAAACGTCAGCGGGTAACACTGGCTGGCGAATCGATTACTGGCTAATCCATAAATGCTTTTTGCCTGCGACGGC
AATCAATAAGGAACAGATTA

Downstream 100 bases:

>100_bases
GCTTGTACACCTACAAGATAATATTATTCATTGAAGGCGCCCACAGAGCGCCTACGACTTCTAGGGATCTGATATGAGTT
TCACCAAAGCGCCACTGTTA

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MIKLGIVMDPISEINIKKDSSFAMLMAAQERGYQLFYMEMADLAMVNGVAMGNMRPLKVINDANKWFELGDAQDTPLSEL
NVVLMRKDPPFDTEFIYATYMLERAEEQGVLIVNKPQSLRDANEKLFTAWFSEFTPETIVTRDANRIRAFHQAKGDIILK
PLDGMGGTSIFRVKQDDPNLGVIIETLTQYGNQYAMAQAFIPEITKGDKRILVVDGEPVPYALARIPKKGETRGNLAAGG
SGVAQPLSESDWKIARAIGPELKKRGLIFVGLDVIGDKLTEINVTSPTCIREIQAAFDVDITGMLFDAIEARLAQ

Sequences:

>Translated_315_residues
MIKLGIVMDPISEINIKKDSSFAMLMAAQERGYQLFYMEMADLAMVNGVAMGNMRPLKVINDANKWFELGDAQDTPLSEL
NVVLMRKDPPFDTEFIYATYMLERAEEQGVLIVNKPQSLRDANEKLFTAWFSEFTPETIVTRDANRIRAFHQAKGDIILK
PLDGMGGTSIFRVKQDDPNLGVIIETLTQYGNQYAMAQAFIPEITKGDKRILVVDGEPVPYALARIPKKGETRGNLAAGG
SGVAQPLSESDWKIARAIGPELKKRGLIFVGLDVIGDKLTEINVTSPTCIREIQAAFDVDITGMLFDAIEARLAQ
>Mature_315_residues
MIKLGIVMDPISEINIKKDSSFAMLMAAQERGYQLFYMEMADLAMVNGVAMGNMRPLKVINDANKWFELGDAQDTPLSEL
NVVLMRKDPPFDTEFIYATYMLERAEEQGVLIVNKPQSLRDANEKLFTAWFSEFTPETIVTRDANRIRAFHQAKGDIILK
PLDGMGGTSIFRVKQDDPNLGVIIETLTQYGNQYAMAQAFIPEITKGDKRILVVDGEPVPYALARIPKKGETRGNLAAGG
SGVAQPLSESDWKIARAIGPELKKRGLIFVGLDVIGDKLTEINVTSPTCIREIQAAFDVDITGMLFDAIEARLAQ

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=315, Percent_Identity=72.0634920634921, Blast_Score=478, Evalue=1e-136,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 34922; Mature: 34922

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLGIVMDPISEINIKKDSSFAMLMAAQERGYQLFYMEMADLAMVNGVAMGNMRPLKVI
CEEEEEEECCHHHCCCCCCCCEEEEEEEHHCCCEEEEEEHHHHHHHCCEEECCCCCEEEE
NDANKWFELGDAQDTPLSELNVVLMRKDPPFDTEFIYATYMLERAEEQGVLIVNKPQSLR
CCCCHHEECCCCCCCCHHHEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHH
DANEKLFTAWFSEFTPETIVTRDANRIRAFHQAKGDIILKPLDGMGGTSIFRVKQDDPNL
HHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHCCCCEEEEECCCCCCCEEEEEECCCCCC
GVIIETLTQYGNQYAMAQAFIPEITKGDKRILVVDGEPVPYALARIPKKGETRGNLAAGG
CHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHCCCCCCCCCCCCCCCC
SGVAQPLSESDWKIARAIGPELKKRGLIFVGLDVIGDKLTEINVTSPTCIREIQAAFDVD
CCCCCCCCCCCCHHHHHHCHHHHHCCEEEEEHHHHCCCEEEEECCCHHHHHHHHHHHCCC
ITGMLFDAIEARLAQ
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MIKLGIVMDPISEINIKKDSSFAMLMAAQERGYQLFYMEMADLAMVNGVAMGNMRPLKVI
CEEEEEEECCHHHCCCCCCCCEEEEEEEHHCCCEEEEEEHHHHHHHCCEEECCCCCEEEE
NDANKWFELGDAQDTPLSELNVVLMRKDPPFDTEFIYATYMLERAEEQGVLIVNKPQSLR
CCCCHHEECCCCCCCCHHHEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHH
DANEKLFTAWFSEFTPETIVTRDANRIRAFHQAKGDIILKPLDGMGGTSIFRVKQDDPNL
HHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHCCCCEEEEECCCCCCCEEEEEECCCCCC
GVIIETLTQYGNQYAMAQAFIPEITKGDKRILVVDGEPVPYALARIPKKGETRGNLAAGG
CHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHCCCCCCCCCCCCCCCC
SGVAQPLSESDWKIARAIGPELKKRGLIFVGLDVIGDKLTEINVTSPTCIREIQAAFDVD
CCCCCCCCCCCCHHHHHHCHHHHHCCEEEEEHHHHCCCEEEEECCCHHHHHHHHHHHCCC
ITGMLFDAIEARLAQ
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]