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The map label for this gene is phoB [H]

Identifier: 117921883

GI number: 117921883

Start: 4128304

End: 4129698

Strand: Direct

Name: phoB [H]

Synonym: Shewana3_3446

Alternate gene names: 117921883

Gene position: 4128304-4129698 (Clockwise)

Preceding gene: 117921882

Following gene: 117921884

Centisome position: 83.03

GC content: 50.75

Gene sequence:

>1395_bases
ATGAGTTTCACCAAAGCGCCACTGTTATTGCTTGGTATGAGTTTATGGCTCTCCAACTCTGCATTGGCAGTCGAAGCGGG
AGCGAATGAGACTGGCCCAGTAAAAGCCCCCTCACGCCCGAAAAATATTGTGATCATGATTGGCGACGGCATGGGGCCTT
CCTATACCAGCGCCTATCGTTATTACAAGGACAATCCAGATACCGAAGAAGTCGAACAAACCGTCTTCGACCGCTTATTG
GTTGGCATGGCGAGCACTTATCCCGCCAGCGTCAGTGGTTATGTCACCGACTCGGCCGCCGCCGCGACTGCCCTCGCGAC
AGGAGTCAAATCCTATAACGGCGCCATCTCGGTCGATACCCAAAAACAGCCACTACCTACCATTTTTGAAAAGGCCAAAA
CCTTAGGCTTGAGCACGGGTGTGGCGGTCACGTCGCAAATCAACCATGCCACGCCAGCGGCCTTTTTATCCCATAACGAG
AGCCGTAAAAACTACGACGCACTGGCATTAAGTTATCTTGAAACCAACGCCGATGTGTTTTTAGGTGGCGGGCAAAAGTA
CTTTCCTCCAGAGCTGTTAGCCCAGTTCACCGCCAAGGGTTATCAACACATTACCCGCTTTGACGATCTGGCCAGCATTA
CCCAGCCAAAGGTCTTGGGTTTGTTTGCCGAGGTGCAACTGCCTTGGGCTATTGATGAAAAAGATGCTAAAAAACTCAGC
ACCTTGACTCAAAAAGCCCTCAGTTTACTGTCGCAAAATGAGCAAGGCTTTGTGCTGCTGGTCGAAGGTAGCCTTATCGA
CTGGGCGGGACACAACAATGATATCGCCGCCGCCATGGGCGAAATGGATGAATTTGCCAATGCAATTGAAGTCGTCGAGC
AATTCGTACGCCAAAACCCTGATACCTTAATGGTGATCACCGCCGACCATAATACTGGCGGGTTATCGATTGGTGCCGAT
GGCAACTACAACTGGAATCCAGAGATCCTACGTAATATCTCGGCAAGCTCAGATACCTTAGCTCAAGCCGCTCTTGGCGG
TGATACTTGGCAAGCAGACCTATCCCGCGGGCTAGGCTTTGAATTGACCGAAGAAGAAGTGACTAAGTTAAATGTTGCCA
GAATGCAGGGCTTAGAAACCATGGCCGTTGCGATTCGCCATGTGATTGATAAACGTACAGATACGGGTTGGACAACCGGC
GGCCACACGGGGACTGATGTGCAAGTTTTTGCCGCAGGCCCAGCCTCTGAGTTGTTTAACGGTCATCAAGACAACACAGA
TATCGCCAATAAGATTTTTAGCCTGTTACCTAAGCCTAAAAAGCCAAAAGCCACCGCACAGGTATCGACTCCCGCTCCAG
CACCAGAACTAGCACCAGTGCAGTCTCAGAGCTAA

Upstream 100 bases:

>100_bases
GCCATCGAAGCCCGTCTTGCCCAGTAAGCTTGTACACCTACAAGATAATATTATTCATTGAAGGCGCCCACAGAGCGCCT
ACGACTTCTAGGGATCTGAT

Downstream 100 bases:

>100_bases
TTTGCTGCGATTAGCTAATAGATGAAAGGCAACCGTGTTAACACTGTTGCCTTTTTTTATTGGCTTTCATTCCACACCAT
TCTAGTGAGCGCTGTTTTGC

Product: alkaline phosphatase

Products: NA

Alternate protein names: Alkaline phosphatase III; APase III [H]

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MSFTKAPLLLLGMSLWLSNSALAVEAGANETGPVKAPSRPKNIVIMIGDGMGPSYTSAYRYYKDNPDTEEVEQTVFDRLL
VGMASTYPASVSGYVTDSAAAATALATGVKSYNGAISVDTQKQPLPTIFEKAKTLGLSTGVAVTSQINHATPAAFLSHNE
SRKNYDALALSYLETNADVFLGGGQKYFPPELLAQFTAKGYQHITRFDDLASITQPKVLGLFAEVQLPWAIDEKDAKKLS
TLTQKALSLLSQNEQGFVLLVEGSLIDWAGHNNDIAAAMGEMDEFANAIEVVEQFVRQNPDTLMVITADHNTGGLSIGAD
GNYNWNPEILRNISASSDTLAQAALGGDTWQADLSRGLGFELTEEEVTKLNVARMQGLETMAVAIRHVIDKRTDTGWTTG
GHTGTDVQVFAAGPASELFNGHQDNTDIANKIFSLLPKPKKPKATAQVSTPAPAPELAPVQSQS

Sequences:

>Translated_464_residues
MSFTKAPLLLLGMSLWLSNSALAVEAGANETGPVKAPSRPKNIVIMIGDGMGPSYTSAYRYYKDNPDTEEVEQTVFDRLL
VGMASTYPASVSGYVTDSAAAATALATGVKSYNGAISVDTQKQPLPTIFEKAKTLGLSTGVAVTSQINHATPAAFLSHNE
SRKNYDALALSYLETNADVFLGGGQKYFPPELLAQFTAKGYQHITRFDDLASITQPKVLGLFAEVQLPWAIDEKDAKKLS
TLTQKALSLLSQNEQGFVLLVEGSLIDWAGHNNDIAAAMGEMDEFANAIEVVEQFVRQNPDTLMVITADHNTGGLSIGAD
GNYNWNPEILRNISASSDTLAQAALGGDTWQADLSRGLGFELTEEEVTKLNVARMQGLETMAVAIRHVIDKRTDTGWTTG
GHTGTDVQVFAAGPASELFNGHQDNTDIANKIFSLLPKPKKPKATAQVSTPAPAPELAPVQSQS
>Mature_463_residues
SFTKAPLLLLGMSLWLSNSALAVEAGANETGPVKAPSRPKNIVIMIGDGMGPSYTSAYRYYKDNPDTEEVEQTVFDRLLV
GMASTYPASVSGYVTDSAAAATALATGVKSYNGAISVDTQKQPLPTIFEKAKTLGLSTGVAVTSQINHATPAAFLSHNES
RKNYDALALSYLETNADVFLGGGQKYFPPELLAQFTAKGYQHITRFDDLASITQPKVLGLFAEVQLPWAIDEKDAKKLST
LTQKALSLLSQNEQGFVLLVEGSLIDWAGHNNDIAAAMGEMDEFANAIEVVEQFVRQNPDTLMVITADHNTGGLSIGADG
NYNWNPEILRNISASSDTLAQAALGGDTWQADLSRGLGFELTEEEVTKLNVARMQGLETMAVAIRHVIDKRTDTGWTTGG
HTGTDVQVFAAGPASELFNGHQDNTDIANKIFSLLPKPKKPKATAQVSTPAPAPELAPVQSQS

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266296, Length=481, Percent_Identity=29.3139293139293, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI94721246, Length=481, Percent_Identity=28.2744282744283, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI116734717, Length=463, Percent_Identity=27.8617710583153, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI294660770, Length=459, Percent_Identity=27.6688453159041, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI157266292, Length=508, Percent_Identity=27.1653543307087, Blast_Score=106, Evalue=4e-23,
Organism=Escherichia coli, GI48994877, Length=334, Percent_Identity=35.3293413173653, Blast_Score=157, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6320689, Length=435, Percent_Identity=31.264367816092, Blast_Score=164, Evalue=2e-41,
Organism=Drosophila melanogaster, GI21355981, Length=324, Percent_Identity=29.9382716049383, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24651554, Length=462, Percent_Identity=29.2207792207792, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI21355149, Length=454, Percent_Identity=27.0925110132159, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI19921912, Length=461, Percent_Identity=26.4642082429501, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI18859923, Length=410, Percent_Identity=26.5853658536585, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI85725200, Length=443, Percent_Identity=25.7336343115124, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI85815833, Length=443, Percent_Identity=25.7336343115124, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI21355151, Length=454, Percent_Identity=24.6696035242291, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21358067, Length=442, Percent_Identity=26.0180995475113, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24657835, Length=453, Percent_Identity=25.60706401766, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24657842, Length=457, Percent_Identity=25.164113785558, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24657827, Length=334, Percent_Identity=26.3473053892216, Blast_Score=84, Evalue=3e-16,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 49611; Mature: 49480

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00123 ALKALINE_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFTKAPLLLLGMSLWLSNSALAVEAGANETGPVKAPSRPKNIVIMIGDGMGPSYTSAYR
CCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHH
YYKDNPDTEEVEQTVFDRLLVGMASTYPASVSGYVTDSAAAATALATGVKSYNGAISVDT
EECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECHHHHHHHHHHHHHHCCCEEEECC
QKQPLPTIFEKAKTLGLSTGVAVTSQINHATPAAFLSHNESRKNYDALALSYLETNADVF
CCCCCHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHCCCCCCCCHHHHEEEEEECCCCEE
LGGGQKYFPPELLAQFTAKGYQHITRFDDLASITQPKVLGLFAEVQLPWAIDEKDAKKLS
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEEECCEEECHHHHHHHH
TLTQKALSLLSQNEQGFVLLVEGSLIDWAGHNNDIAAAMGEMDEFANAIEVVEQFVRQNP
HHHHHHHHHHHCCCCCEEEEEECCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
DTLMVITADHNTGGLSIGADGNYNWNPEILRNISASSDTLAQAALGGDTWQADLSRGLGF
CEEEEEEECCCCCCEEECCCCCCCCCHHHHHHCCCCCHHHHHHHHCCCCHHHHHHCCCCC
ELTEEEVTKLNVARMQGLETMAVAIRHVIDKRTDTGWTTGGHTGTDVQVFAAGPASELFN
EECHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHCC
GHQDNTDIANKIFSLLPKPKKPKATAQVSTPAPAPELAPVQSQS
CCCCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SFTKAPLLLLGMSLWLSNSALAVEAGANETGPVKAPSRPKNIVIMIGDGMGPSYTSAYR
CCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHH
YYKDNPDTEEVEQTVFDRLLVGMASTYPASVSGYVTDSAAAATALATGVKSYNGAISVDT
EECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECHHHHHHHHHHHHHHCCCEEEECC
QKQPLPTIFEKAKTLGLSTGVAVTSQINHATPAAFLSHNESRKNYDALALSYLETNADVF
CCCCCHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHCCCCCCCCHHHHEEEEEECCCCEE
LGGGQKYFPPELLAQFTAKGYQHITRFDDLASITQPKVLGLFAEVQLPWAIDEKDAKKLS
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEEECCEEECHHHHHHHH
TLTQKALSLLSQNEQGFVLLVEGSLIDWAGHNNDIAAAMGEMDEFANAIEVVEQFVRQNP
HHHHHHHHHHHCCCCCEEEEEECCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
DTLMVITADHNTGGLSIGADGNYNWNPEILRNISASSDTLAQAALGGDTWQADLSRGLGF
CEEEEEEECCCCCCEEECCCCCCCCCHHHHHHCCCCCHHHHHHHHCCCCHHHHHHCCCCC
ELTEEEVTKLNVARMQGLETMAVAIRHVIDKRTDTGWTTGGHTGTDVQVFAAGPASELFN
EECHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHCC
GHQDNTDIANKIFSLLPKPKKPKATAQVSTPAPAPELAPVQSQS
CCCCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1898729; 9202461; 9384377; 2113910; 2105301 [H]