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The map label for this gene is ldhA [H]

Identifier: 117921757

GI number: 117921757

Start: 3980612

End: 3981601

Strand: Direct

Name: ldhA [H]

Synonym: Shewana3_3319

Alternate gene names: 117921757

Gene position: 3980612-3981601 (Clockwise)

Preceding gene: 117921756

Following gene: 117921758

Centisome position: 80.06

GC content: 49.39

Gene sequence:

>990_bases
ATGAGAATTGGATTTTTTAGCGCAAAACACTACGACATGCAGCATTTTAACCGTACAAATGCCGCATTTGGTGCGCAAAT
TGAGTATTTCGATTATCGCCTCTGCATGCAAACCGTTAAGCTTGCCGAAGGTTTTGAGGTGGTGTGCGCCTTTGTTAACG
ATTCCCTCTGTGAAGAAGTGTTAGTCGAGCTTGCCAAAGGTGGCACGAAAATCATTGCCATGCGCTGCGCGGGTTTTAAC
AATGTCGACTTAGTCGCCGCCAAACGCTTAGGCATGCAAGTGGTCAACGTGCCCGCCTATTCGCCCGAATCTGTAGCCGA
GCACACAGTCGCCTTGATGCTGACCCTAAACCGCAAAATCCATAAGGCCTACCAACGCACCCGTGATGCGAACTTCTCCC
TCGAAGGCTTAGTCGGTTTTAACATGTTTGGCAAAACCGTTGGCGTAATAGGCACAGGTAAAATCGGGGTCGCCACGATT
AAGGTCTTGCTCGGTTTTGGCTGTAAAGTCATAGCCTTTGACCCCTACCCAAATCCTGCGGTAGAAGCGCTCAATGTGGA
ATATCAGGATCTGGACACCATTTACGCTAACAGCGATATTATCAGCCTGCACTGCCCATTAACCGCCGATAACCACCATT
TGCTGAATAAAGAAAGCTTTGCCAAGATGAAACCCGGCGTCATGGTGATCAACACTAGCCGCGGCGGCCTGCTCAATGCC
TTCGATGCGATGGAAGCCTTAAAGCTCGGCCAGATAGGCTCATTAGGGCTTGATGTGTATGAAAATGAAAAAGAACTGTT
TTTCGAGGACAAGTCGAACCAGATCATTCAGGACGATGTGTTCCGTCGCTTATCTGCATGTCATAACGTGATTTTTACCG
GTCACCAGGCCTTCCTTACCGAAGAGGCGCTAGGCGCGATTGCACATACAACCCTGAGCAACGTCAAAGCCCTACTCGCG
GGCGAGCGCTCTGGTAACGAACTGTTTTAA

Upstream 100 bases:

>100_bases
AGCTCACTCTTTTTACGGGGTAAATTCATCACCAGAGGACAAATGTCGCCGAGTCACATACACTCAGTGCCAGTAACAAT
GTAACCTTTAGGTGATTGAA

Downstream 100 bases:

>100_bases
CTGACAGAACCATACCTGACTGCTAAAGTGAGGCTTCAACATTGAAGCCTCACAGAGGAAACTAAATATGTTAGTGACTC
AGCAAACTCAGAATATCAAC

Product: D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding

Products: NA

Alternate protein names: D-LDH; Fermentative lactate dehydrogenase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MRIGFFSAKHYDMQHFNRTNAAFGAQIEYFDYRLCMQTVKLAEGFEVVCAFVNDSLCEEVLVELAKGGTKIIAMRCAGFN
NVDLVAAKRLGMQVVNVPAYSPESVAEHTVALMLTLNRKIHKAYQRTRDANFSLEGLVGFNMFGKTVGVIGTGKIGVATI
KVLLGFGCKVIAFDPYPNPAVEALNVEYQDLDTIYANSDIISLHCPLTADNHHLLNKESFAKMKPGVMVINTSRGGLLNA
FDAMEALKLGQIGSLGLDVYENEKELFFEDKSNQIIQDDVFRRLSACHNVIFTGHQAFLTEEALGAIAHTTLSNVKALLA
GERSGNELF

Sequences:

>Translated_329_residues
MRIGFFSAKHYDMQHFNRTNAAFGAQIEYFDYRLCMQTVKLAEGFEVVCAFVNDSLCEEVLVELAKGGTKIIAMRCAGFN
NVDLVAAKRLGMQVVNVPAYSPESVAEHTVALMLTLNRKIHKAYQRTRDANFSLEGLVGFNMFGKTVGVIGTGKIGVATI
KVLLGFGCKVIAFDPYPNPAVEALNVEYQDLDTIYANSDIISLHCPLTADNHHLLNKESFAKMKPGVMVINTSRGGLLNA
FDAMEALKLGQIGSLGLDVYENEKELFFEDKSNQIIQDDVFRRLSACHNVIFTGHQAFLTEEALGAIAHTTLSNVKALLA
GERSGNELF
>Mature_329_residues
MRIGFFSAKHYDMQHFNRTNAAFGAQIEYFDYRLCMQTVKLAEGFEVVCAFVNDSLCEEVLVELAKGGTKIIAMRCAGFN
NVDLVAAKRLGMQVVNVPAYSPESVAEHTVALMLTLNRKIHKAYQRTRDANFSLEGLVGFNMFGKTVGVIGTGKIGVATI
KVLLGFGCKVIAFDPYPNPAVEALNVEYQDLDTIYANSDIISLHCPLTADNHHLLNKESFAKMKPGVMVINTSRGGLLNA
FDAMEALKLGQIGSLGLDVYENEKELFFEDKSNQIIQDDVFRRLSACHNVIFTGHQAFLTEEALGAIAHTTLSNVKALLA
GERSGNELF

Specific function: Fermentative lactate dehydrogenase [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=195, Percent_Identity=34.8717948717949, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI61743967, Length=243, Percent_Identity=33.7448559670782, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI4557497, Length=243, Percent_Identity=33.7448559670782, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI145580578, Length=262, Percent_Identity=31.6793893129771, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI4557499, Length=262, Percent_Identity=31.6793893129771, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI145580575, Length=244, Percent_Identity=33.6065573770492, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI6912396, Length=252, Percent_Identity=29.3650793650794, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1787645, Length=328, Percent_Identity=62.5, Blast_Score=440, Evalue=1e-125,
Organism=Escherichia coli, GI87082289, Length=193, Percent_Identity=32.1243523316062, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1789279, Length=228, Percent_Identity=27.6315789473684, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788660, Length=222, Percent_Identity=28.8288288288288, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17532191, Length=210, Percent_Identity=34.7619047619048, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI25147481, Length=233, Percent_Identity=32.618025751073, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324055, Length=199, Percent_Identity=33.6683417085427, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6320925, Length=238, Percent_Identity=31.5126050420168, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6322116, Length=238, Percent_Identity=31.0924369747899, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6324964, Length=204, Percent_Identity=28.4313725490196, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6324980, Length=140, Percent_Identity=27.8571428571429, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24646446, Length=261, Percent_Identity=32.183908045977, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24646448, Length=261, Percent_Identity=32.183908045977, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24646452, Length=261, Percent_Identity=32.183908045977, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24646450, Length=261, Percent_Identity=32.183908045977, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI62472511, Length=261, Percent_Identity=32.183908045977, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI19921140, Length=271, Percent_Identity=28.4132841328413, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI28574286, Length=275, Percent_Identity=30.1818181818182, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI45552429, Length=259, Percent_Identity=30.5019305019305, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24585514, Length=259, Percent_Identity=30.5019305019305, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI28574282, Length=259, Percent_Identity=30.5019305019305, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI28574284, Length=259, Percent_Identity=30.5019305019305, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI45551003, Length=259, Percent_Identity=30.5019305019305, Blast_Score=98, Evalue=8e-21,
Organism=Drosophila melanogaster, GI28571528, Length=293, Percent_Identity=26.962457337884, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24585516, Length=208, Percent_Identity=23.0769230769231, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.28 [H]

Molecular weight: Translated: 36186; Mature: 36186

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGFFSAKHYDMQHFNRTNAAFGAQIEYFDYRLCMQTVKLAEGFEVVCAFVNDSLCEEV
CEEEEECCCCCCHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LVELAKGGTKIIAMRCAGFNNVDLVAAKRLGMQVVNVPAYSPESVAEHTVALMLTLNRKI
HHHHHCCCCEEEEEEECCCCCCHHHHHHHCCEEEEECCCCCHHHHHHHHHHHEEHHHHHH
HKAYQRTRDANFSLEGLVGFNMFGKTVGVIGTGKIGVATIKVLLGFGCKVIAFDPYPNPA
HHHHHHHCCCCCEEEEEEEHHHHCCEEEEEECCCHHHHHHHHHHHCCCEEEEECCCCCHH
VEALNVEYQDLDTIYANSDIISLHCPLTADNHHLLNKESFAKMKPGVMVINTSRGGLLNA
HHEECCCHHHHHEEEECCCEEEEECCCCCCCCCCCCCHHHHHCCCCEEEEECCCCCCHHH
FDAMEALKLGQIGSLGLDVYENEKELFFEDKSNQIIQDDVFRRLSACHNVIFTGHQAFLT
HHHHHHHHCCCCCCCCCEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHEECCCHHHHH
EEALGAIAHTTLSNVKALLAGERSGNELF
HHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRIGFFSAKHYDMQHFNRTNAAFGAQIEYFDYRLCMQTVKLAEGFEVVCAFVNDSLCEEV
CEEEEECCCCCCHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LVELAKGGTKIIAMRCAGFNNVDLVAAKRLGMQVVNVPAYSPESVAEHTVALMLTLNRKI
HHHHHCCCCEEEEEEECCCCCCHHHHHHHCCEEEEECCCCCHHHHHHHHHHHEEHHHHHH
HKAYQRTRDANFSLEGLVGFNMFGKTVGVIGTGKIGVATIKVLLGFGCKVIAFDPYPNPA
HHHHHHHCCCCCEEEEEEEHHHHCCEEEEEECCCHHHHHHHHHHHCCCEEEEECCCCCHH
VEALNVEYQDLDTIYANSDIISLHCPLTADNHHLLNKESFAKMKPGVMVINTSRGGLLNA
HHEECCCHHHHHEEEECCCEEEEECCCCCCCCCCCCCHHHHHCCCCEEEEECCCCCCHHH
FDAMEALKLGQIGSLGLDVYENEKELFFEDKSNQIIQDDVFRRLSACHNVIFTGHQAFLT
HHHHHHHHCCCCCCCCCEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHEECCCHHHHH
EEALGAIAHTTLSNVKALLAGERSGNELF
HHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9025293; 9097039; 9278503; 8349564 [H]