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The map label for this gene is hprA [H]

Identifier: 117921639

GI number: 117921639

Start: 3844358

End: 3845311

Strand: Reverse

Name: hprA [H]

Synonym: Shewana3_3201

Alternate gene names: 117921639

Gene position: 3845311-3844358 (Counterclockwise)

Preceding gene: 117921640

Following gene: 117921634

Centisome position: 77.34

GC content: 52.73

Gene sequence:

>954_bases
ATGAAAATAGTCGTACTCGATGGTGAAACCTTAAATCCTGGGGATCTAACCTGGCAAGCGGTCAGCGCATTAGGGGAATT
TAGCTGTTTTGCTCGCACGCCCTCGGCGGAGATTATCCCCCGCGCTCAGCATGCCGAAATTGTGCTCACCAACAAGACGC
CCCTCGATGCCAACACCTTAGCCCAACTGCCTAAGCTTAAATATATCGGCGTGCTCGCCACCGGCACTAACGTGGTGGAT
CTTGCCGCCGCAAAAGAGTTAGGCATAGTGGTCACCAACGTGCCCGCCTATGGCCCCGATGCCGTCGCACAAATGGTGTT
TGCCCATATTTTGCATCACACGCAAGCCGTTGCGGCGCACCATCAAGCCGTTGCCGCAGGGCAATGGAGTAATTGCAGTG
ACTTTTGTTTTACTTTAATGCCGCTGCAATCCCTTAAAGGTAAAACCTTAGGCTTGATTGGTTATGGGGATATTGGCCAG
CAGGTGGCTAAACTCGCTTTAGCTTTCGGTATGAAGGTATTAGTCAATACCCGCACTCAGCCCCGCGATTTACCCCAAGG
CGTGAGTTGGACATCACGCGATACGGTATTCAAGGAGTCCGATATTCTCTCGCTCCACTGTCCACTTACCCCTGAAACCA
CCGAGCTGATTAATGCCCAAACCCTCGAGTTAATGAAGCCGCAGGCGCTACTGATCAACACCGCACGCGGCGGCTTAATC
GATGAGGCCGCGCTGGCTACGGCATTAAGGCAGGGCAAAGTCTTTGCTGGCGTCGATGTTCTCTCAACCGAACCACCGAG
CGCGGATAATCCGCTACTAACAGCGCCCAATATCAGTATCAGCCCACACAATGCTTGGGCGACCAAAGAAGCGAGGCAGA
ACCTACTCAATATCGCAACGGCAAATCTCAGCGCTTATCTAGCTGGCGAGCGAGTTAATTGGGTGAATCCATAA

Upstream 100 bases:

>100_bases
TCGGATGTAATGTGGCAAGTGCAAGCCAATCTTGGGGTCAAACTCAGTTTTTAATTTTCCTGCTTTATGGGCAAGGCGCA
AACGCAGTTAAGAGGTAAAG

Downstream 100 bases:

>100_bases
GGCCTGTGGGTTAGCTTGTTTCTGTAGGAAATGTTTTCATTCCAAAGAAATGCAGTTGGAATTGAAGGGGGAAAAATGTG
TTGGATAGTTCCCTTAACGG

Product: D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding

Products: NA

Alternate protein names: GDH; Glyoxylate reductase; Hydroxypyruvate dehydrogenase; NADH-dependent hydroxypyruvate reductase; HPR; HPR-A [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MKIVVLDGETLNPGDLTWQAVSALGEFSCFARTPSAEIIPRAQHAEIVLTNKTPLDANTLAQLPKLKYIGVLATGTNVVD
LAAAKELGIVVTNVPAYGPDAVAQMVFAHILHHTQAVAAHHQAVAAGQWSNCSDFCFTLMPLQSLKGKTLGLIGYGDIGQ
QVAKLALAFGMKVLVNTRTQPRDLPQGVSWTSRDTVFKESDILSLHCPLTPETTELINAQTLELMKPQALLINTARGGLI
DEAALATALRQGKVFAGVDVLSTEPPSADNPLLTAPNISISPHNAWATKEARQNLLNIATANLSAYLAGERVNWVNP

Sequences:

>Translated_317_residues
MKIVVLDGETLNPGDLTWQAVSALGEFSCFARTPSAEIIPRAQHAEIVLTNKTPLDANTLAQLPKLKYIGVLATGTNVVD
LAAAKELGIVVTNVPAYGPDAVAQMVFAHILHHTQAVAAHHQAVAAGQWSNCSDFCFTLMPLQSLKGKTLGLIGYGDIGQ
QVAKLALAFGMKVLVNTRTQPRDLPQGVSWTSRDTVFKESDILSLHCPLTPETTELINAQTLELMKPQALLINTARGGLI
DEAALATALRQGKVFAGVDVLSTEPPSADNPLLTAPNISISPHNAWATKEARQNLLNIATANLSAYLAGERVNWVNP
>Mature_317_residues
MKIVVLDGETLNPGDLTWQAVSALGEFSCFARTPSAEIIPRAQHAEIVLTNKTPLDANTLAQLPKLKYIGVLATGTNVVD
LAAAKELGIVVTNVPAYGPDAVAQMVFAHILHHTQAVAAHHQAVAAGQWSNCSDFCFTLMPLQSLKGKTLGLIGYGDIGQ
QVAKLALAFGMKVLVNTRTQPRDLPQGVSWTSRDTVFKESDILSLHCPLTPETTELINAQTLELMKPQALLINTARGGLI
DEAALATALRQGKVFAGVDVLSTEPPSADNPLLTAPNISISPHNAWATKEARQNLLNIATANLSAYLAGERVNWVNP

Specific function: Plays a central role in assimilation of carbon. It converts hydroxypyruvate to glycerate as a key step in the serine cycle, and may also play an important role in C2 reactions, by interconverting glyoxylate and glycolate [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI145580578, Length=263, Percent_Identity=34.9809885931559, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI4557499, Length=263, Percent_Identity=34.9809885931559, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI61743967, Length=263, Percent_Identity=34.6007604562738, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI4557497, Length=263, Percent_Identity=34.6007604562738, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI145580575, Length=263, Percent_Identity=34.9809885931559, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI23308577, Length=263, Percent_Identity=32.319391634981, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI6912396, Length=251, Percent_Identity=31.8725099601594, Blast_Score=108, Evalue=5e-24,
Organism=Escherichia coli, GI1787645, Length=270, Percent_Identity=35.9259259259259, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI87082289, Length=280, Percent_Identity=33.2142857142857, Blast_Score=134, Evalue=6e-33,
Organism=Escherichia coli, GI1789279, Length=256, Percent_Identity=34.375, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI87081824, Length=177, Percent_Identity=31.0734463276836, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788660, Length=280, Percent_Identity=29.2857142857143, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17532191, Length=284, Percent_Identity=29.9295774647887, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI25147481, Length=254, Percent_Identity=30.3149606299213, Blast_Score=104, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6320925, Length=300, Percent_Identity=33.3333333333333, Blast_Score=136, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6322116, Length=300, Percent_Identity=32.6666666666667, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6324055, Length=234, Percent_Identity=30.7692307692308, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324964, Length=303, Percent_Identity=28.0528052805281, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6321253, Length=182, Percent_Identity=30.7692307692308, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI28571528, Length=288, Percent_Identity=32.9861111111111, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI28574286, Length=276, Percent_Identity=31.8840579710145, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24646446, Length=262, Percent_Identity=33.969465648855, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24646448, Length=262, Percent_Identity=33.969465648855, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24646452, Length=262, Percent_Identity=33.969465648855, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24646450, Length=262, Percent_Identity=33.969465648855, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI62472511, Length=262, Percent_Identity=33.969465648855, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI28574284, Length=282, Percent_Identity=30.8510638297872, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI45552429, Length=282, Percent_Identity=30.8510638297872, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24585514, Length=282, Percent_Identity=30.8510638297872, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI28574282, Length=282, Percent_Identity=30.8510638297872, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI45551003, Length=282, Percent_Identity=30.8510638297872, Blast_Score=121, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24585516, Length=234, Percent_Identity=30.7692307692308, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI19921140, Length=253, Percent_Identity=29.2490118577075, Blast_Score=101, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.29 [H]

Molecular weight: Translated: 33804; Mature: 33804

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVVLDGETLNPGDLTWQAVSALGEFSCFARTPSAEIIPRAQHAEIVLTNKTPLDANTL
CEEEEECCCCCCCCCHHHHHHHHHHHHHHEECCCCHHHCCCCCCCEEEEECCCCCCHHHH
AQLPKLKYIGVLATGTNVVDLAAAKELGIVVTNVPAYGPDAVAQMVFAHILHHTQAVAAH
HHCCCEEEEEEEECCCCEEEHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
HQAVAAGQWSNCSDFCFTLMPLQSLKGKTLGLIGYGDIGQQVAKLALAFGMKVLVNTRTQ
HHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCEEEEECCCC
PRDLPQGVSWTSRDTVFKESDILSLHCPLTPETTELINAQTLELMKPQALLINTARGGLI
CCCCCCCCCCCCCCCEEECCCEEEEECCCCCCHHHHHCHHHHHHCCCCEEEEECCCCCCH
DEAALATALRQGKVFAGVDVLSTEPPSADNPLLTAPNISISPHNAWATKEARQNLLNIAT
HHHHHHHHHHCCCEEECEEEECCCCCCCCCCEEECCCCEECCCCCHHHHHHHHHHHHHHH
ANLSAYLAGERVNWVNP
HCEEEEEECCEECCCCC
>Mature Secondary Structure
MKIVVLDGETLNPGDLTWQAVSALGEFSCFARTPSAEIIPRAQHAEIVLTNKTPLDANTL
CEEEEECCCCCCCCCHHHHHHHHHHHHHHEECCCCHHHCCCCCCCEEEEECCCCCCHHHH
AQLPKLKYIGVLATGTNVVDLAAAKELGIVVTNVPAYGPDAVAQMVFAHILHHTQAVAAH
HHCCCEEEEEEEECCCCEEEHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
HQAVAAGQWSNCSDFCFTLMPLQSLKGKTLGLIGYGDIGQQVAKLALAFGMKVLVNTRTQ
HHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCEEEEECCCC
PRDLPQGVSWTSRDTVFKESDILSLHCPLTPETTELINAQTLELMKPQALLINTARGGLI
CCCCCCCCCCCCCCCEEECCCEEEEECCCCCCHHHHHCHHHHHHCCCCEEEEECCCCCCH
DEAALATALRQGKVFAGVDVLSTEPPSADNPLLTAPNISISPHNAWATKEARQNLLNIAT
HHHHHHHHHHCCCEEECEEEECCCCCCCCCCEEECCCCEECCCCCHHHHHHHHHHHHHHH
ANLSAYLAGERVNWVNP
HCEEEEEECCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8144463; 1729225; 1657886 [H]