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The map label for this gene is puuA [H]

Identifier: 117921536

GI number: 117921536

Start: 3716755

End: 3718110

Strand: Reverse

Name: puuA [H]

Synonym: Shewana3_3098

Alternate gene names: 117921536

Gene position: 3718110-3716755 (Counterclockwise)

Preceding gene: 117921537

Following gene: 117921535

Centisome position: 74.78

GC content: 49.04

Gene sequence:

>1356_bases
ATGAATAAGCTAATCGCTTTTTTAAAAGAACGAAAAATCACAGAAGTCGAATGTGTTATCAGCGATATGACAGGTATCGC
CCGCGGAAAAATTGCCCCAGTGGATAAGTTCCTCGACGAAAAAGGCATGCGCTTGCCCGAAAGCGTGTTGCTGCAAACCG
TGACCGGTGACTTTGTCGACGATGATATTTATTACAGCTTACTCGACGATGCGGATATCGATTTTGTCTGTGTCCCCGAT
GAAAATGCGGTGTTTATGCTGCCTTGGACCATTGAAGCGACGGCGCAAGTGATCCACGATTGCTACGACAGAATGGGCAA
TCCCATCGAACTATCACCACGTAACGTGTTGAAAAAAGTGCTTTCGCTATACGAGCAAAAAGGCTGGGAGCCAGTCATTG
CACCTGAGATGGAGTTTTATCTCACCAGCCGTAGCGATGACCACGATTTACCCCTTAAGCCACCTATTGGTCGCTCGGGC
AGACCGGAAGCGGGACGTCAGTCTTTCTCGATTGATGCGGCTAACGAATACGACCCGCTTTTTGAAGATATGTATGACTG
GTGCGAGCTGCAAGGTTTAGATATCGATACGCTGATCCACGAAGACGGTCCAGCGCAAATGGAGATTAACTTCAGCCACG
GTAATCCATTATCTCTTGCGGATCAGGTGTTTGTATTCAAGCGTACCCTGCGTGAGGCGGCGTTAAAGCACAATGTGTGC
GCCACCTTTATGGCTAAACCTGTCACCGATGAGCCAGGCAGTGCCATGCACATTCATCAAAGCGTGATTAATAAGGAAAC
GGGCAAAAACATCTTCACTAATGAAGACGGAACTCAAAGCCCATTATTCCTAAGCTATATCGCCGGTTTACAAAAGTTTA
TTCCTGAACTCTTGCCGTTAATGGCGCCTAATGCCAACTCGTTCCGCCGTTTCTTACCGGGCACCTCTGCGCCAGTTAAC
TTGGAATGGGGTGTTGAGAACAGAACCTGCGGTCTGCGTATTCCTGAGTCTTCACCGCAAAACCGCCGCATTGAAAACCG
TATTCCTGGGGCGGATGCGAACTGCTATTTAGCCATAGCTGCGAGCTTACTCTGCGGTTACATCGGTATGGTGGAAGGGT
TAAAACCTTCGACACCAGTGCAGGGTAAATCGAACGAAAGCCGTAGCAACAATCCACATTGCCTGCCGTTAACCTTAGAA
GAAGCCTTAGCGGCGATGGAAGAGAGCGATGCCTGTAAGGAATATTTAGGTGAAGCCTTTACCACCGGCTTTGTGGCGGT
TAAACAGGCCGAGCTTGAAAACTTCCGCCGCGTCGTGAGTTCGTGGGAGCGGGAGTTCCTGTTACTCAGTGTGTAA

Upstream 100 bases:

>100_bases
CATCCTGAATGGAAGGTGAGTGAAAACCCATTTTTCCTCTCTATATTCAATGCCTTTGGTGATGCGTGCCGTCGTAGGGC
TACAACTCGAGTGAAATAAA

Downstream 100 bases:

>100_bases
TTTGCACTGTTTTTGTTCAAAACTGTCACGGCCTTAACGGATTTAGGGTCAAGATAGCGGGAATATTTGAACAATCAGCA
AAAATGCTCTGCACTAATTT

Product: L-glutamine synthetase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MNKLIAFLKERKITEVECVISDMTGIARGKIAPVDKFLDEKGMRLPESVLLQTVTGDFVDDDIYYSLLDDADIDFVCVPD
ENAVFMLPWTIEATAQVIHDCYDRMGNPIELSPRNVLKKVLSLYEQKGWEPVIAPEMEFYLTSRSDDHDLPLKPPIGRSG
RPEAGRQSFSIDAANEYDPLFEDMYDWCELQGLDIDTLIHEDGPAQMEINFSHGNPLSLADQVFVFKRTLREAALKHNVC
ATFMAKPVTDEPGSAMHIHQSVINKETGKNIFTNEDGTQSPLFLSYIAGLQKFIPELLPLMAPNANSFRRFLPGTSAPVN
LEWGVENRTCGLRIPESSPQNRRIENRIPGADANCYLAIAASLLCGYIGMVEGLKPSTPVQGKSNESRSNNPHCLPLTLE
EALAAMEESDACKEYLGEAFTTGFVAVKQAELENFRRVVSSWEREFLLLSV

Sequences:

>Translated_451_residues
MNKLIAFLKERKITEVECVISDMTGIARGKIAPVDKFLDEKGMRLPESVLLQTVTGDFVDDDIYYSLLDDADIDFVCVPD
ENAVFMLPWTIEATAQVIHDCYDRMGNPIELSPRNVLKKVLSLYEQKGWEPVIAPEMEFYLTSRSDDHDLPLKPPIGRSG
RPEAGRQSFSIDAANEYDPLFEDMYDWCELQGLDIDTLIHEDGPAQMEINFSHGNPLSLADQVFVFKRTLREAALKHNVC
ATFMAKPVTDEPGSAMHIHQSVINKETGKNIFTNEDGTQSPLFLSYIAGLQKFIPELLPLMAPNANSFRRFLPGTSAPVN
LEWGVENRTCGLRIPESSPQNRRIENRIPGADANCYLAIAASLLCGYIGMVEGLKPSTPVQGKSNESRSNNPHCLPLTLE
EALAAMEESDACKEYLGEAFTTGFVAVKQAELENFRRVVSSWEREFLLLSV
>Mature_451_residues
MNKLIAFLKERKITEVECVISDMTGIARGKIAPVDKFLDEKGMRLPESVLLQTVTGDFVDDDIYYSLLDDADIDFVCVPD
ENAVFMLPWTIEATAQVIHDCYDRMGNPIELSPRNVLKKVLSLYEQKGWEPVIAPEMEFYLTSRSDDHDLPLKPPIGRSG
RPEAGRQSFSIDAANEYDPLFEDMYDWCELQGLDIDTLIHEDGPAQMEINFSHGNPLSLADQVFVFKRTLREAALKHNVC
ATFMAKPVTDEPGSAMHIHQSVINKETGKNIFTNEDGTQSPLFLSYIAGLQKFIPELLPLMAPNANSFRRFLPGTSAPVN
LEWGVENRTCGLRIPESSPQNRRIENRIPGADANCYLAIAASLLCGYIGMVEGLKPSTPVQGKSNESRSNNPHCLPLTLE
EALAAMEESDACKEYLGEAFTTGFVAVKQAELENFRRVVSSWEREFLLLSV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=450, Percent_Identity=27.5555555555556, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI87081870, Length=439, Percent_Identity=35.3075170842825, Blast_Score=245, Evalue=3e-66,
Organism=Escherichia coli, GI1790301, Length=447, Percent_Identity=25.9507829977629, Blast_Score=116, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 50403; Mature: 50403

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLIAFLKERKITEVECVISDMTGIARGKIAPVDKFLDEKGMRLPESVLLQTVTGDFVD
CHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCC
DDIYYSLLDDADIDFVCVPDENAVFMLPWTIEATAQVIHDCYDRMGNPIELSPRNVLKKV
CHHHHHHHCCCCCCEEEECCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEECHHHHHHHH
LSLYEQKGWEPVIAPEMEFYLTSRSDDHDLPLKPPIGRSGRPEAGRQSFSIDAANEYDPL
HHHHHHCCCCCEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCHH
FEDMYDWCELQGLDIDTLIHEDGPAQMEINFSHGNPLSLADQVFVFKRTLREAALKHNVC
HHHHHHHHHCCCCCHHHEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ATFMAKPVTDEPGSAMHIHQSVINKETGKNIFTNEDGTQSPLFLSYIAGLQKFIPELLPL
HHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
MAPNANSFRRFLPGTSAPVNLEWGVENRTCGLRIPESSPQNRRIENRIPGADANCYLAIA
CCCCHHHHHHHCCCCCCCEEEEECCCCCCCCEECCCCCCCCCCHHHCCCCCCCCHHHHHH
ASLLCGYIGMVEGLKPSTPVQGKSNESRSNNPHCLPLTLEEALAAMEESDACKEYLGEAF
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHH
TTGFVAVKQAELENFRRVVSSWEREFLLLSV
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
>Mature Secondary Structure
MNKLIAFLKERKITEVECVISDMTGIARGKIAPVDKFLDEKGMRLPESVLLQTVTGDFVD
CHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCC
DDIYYSLLDDADIDFVCVPDENAVFMLPWTIEATAQVIHDCYDRMGNPIELSPRNVLKKV
CHHHHHHHCCCCCCEEEECCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEECHHHHHHHH
LSLYEQKGWEPVIAPEMEFYLTSRSDDHDLPLKPPIGRSGRPEAGRQSFSIDAANEYDPL
HHHHHHCCCCCEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCHH
FEDMYDWCELQGLDIDTLIHEDGPAQMEINFSHGNPLSLADQVFVFKRTLREAALKHNVC
HHHHHHHHHCCCCCHHHEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ATFMAKPVTDEPGSAMHIHQSVINKETGKNIFTNEDGTQSPLFLSYIAGLQKFIPELLPL
HHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
MAPNANSFRRFLPGTSAPVNLEWGVENRTCGLRIPESSPQNRRIENRIPGADANCYLAIA
CCCCHHHHHHHCCCCCCCEEEEECCCCCCCCEECCCCCCCCCCHHHCCCCCCCCHHHHHH
ASLLCGYIGMVEGLKPSTPVQGKSNESRSNNPHCLPLTLEEALAAMEESDACKEYLGEAF
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHH
TTGFVAVKQAELENFRRVVSSWEREFLLLSV
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]