Click here to switch to the map view.

The map label for this gene is puuB [H]

Identifier: 117921531

GI number: 117921531

Start: 3710461

End: 3711747

Strand: Reverse

Name: puuB [H]

Synonym: Shewana3_3093

Alternate gene names: 117921531

Gene position: 3711747-3710461 (Counterclockwise)

Preceding gene: 117921532

Following gene: 117921530

Centisome position: 74.65

GC content: 50.12

Gene sequence:

>1287_bases
ATGTCAGCCATACCTCACACTGGATCATATTATGCGGCATCGGCCAACGATAAGGTTGAGCGCGCGCGTTTACAGGAATC
GATTGAAGCCGATGTTTGTGTGATTGGCGCGGGGTACACAGGCCTATCTGCCGCACTGCATTTACTCGAATCGGGTTTTA
GTGTGGTGGTGCTTGAAGCGGCGCGAATTGGCTGGGGAGCCTCGGGGCGTAATGGCGGACAAATCGTCAACAGCTATAGC
CGTGACATTGATACCATTGAAAAAACCGTTGGCAAAGAACAAGCCAAGCTTTTCGGTCAAATGGCCTTCGAGGGCGGGCG
TATCATCCGTGAACGTATCGCTAAGTACAATATTGATTGCGATCTAAAGGATGGCGGTGTATTTGCGGCGATGAACGAAA
AGCAAATGGGCCATTTACGCCATCAAAAGCAATTATGGGAAAGCCATGGCCACGTGGGCAAGCTGGAGCTGCTCGATGCA
AACAGCATTCGCTCTGTGGTAAATACTGAGCGCTATGTCGGCGGTATGTTAGATAAGAGCGGCGGTCATATTCATCCGCT
GAATTTAGCCTTGGGTGAAGCCCGTGCGGTAGAATCCCTCGGCGGAAAAATTTTTGAAGACTCAGCAGTATTGCGGGTCG
ACGAAGGCGATAGTCCTGTGGTGCACACCGCTAAGGGCAGCGTAAAAGCCAAGTTTGTGGTGGTGGCGGGTAACGCTTAC
CTAGGTAAACTCATGCCTGAATTACAAGCTAAATCAATGCCCTGTGGCACCCAGGTGATCACCACAGAACCCTTAGACGA
AGAGCTGGCCGCAAGCCTGTTACCTCAAGATTATTGCGTCGAAGATTGTAACTATCTGCTCGATTATTTTAGGCTGTCTG
GTGACAAGCGTCTGATCTACGGCGGTGGTGTCGTGTATGGTGCCCGCGATCCCGCGGATATCGAATCCCTGATTATCCCG
AATATGCTCAAAACCTTCCCACAGTTAAAAGGGGTTAAGGTTGATTATGCCTGGACGGGTAACTTTTTATTGACCTTATC
GCGTTTACCACAGGTCGGCCGTATTGGTAAAAACATCTATTATTCACAGGGTTGTAGTGGACATGGCGTCACTTATACCC
ACTTGGCAGGCAAGTTAATTGCCGAAATGCTCAATGGTCAAGCCACGCGTTTCGATGCTTTTGCGGCGCTGCCCCATTAT
CCCTTCCCCGGCGGTCACGCCTTACGTGTGCCTTTTAGTGCGCTGGGAGCATGGTATTACAGTCTAAGAGACAAATTAGG
TATCTAA

Upstream 100 bases:

>100_bases
TGTGTAAATTATTGTGCTTTTATGGCTAGTGTTTCCCGATTTGAAGGTGTAGGATGTGATAGTTGTTTAAAATAATGAAC
ATAAAAATCAAGGGAACATC

Downstream 100 bases:

>100_bases
TTTATTTAATTTTTTATACGAGGTAAGGCATTACCTCGCAGCGAGAGGAATTTCCTGTGTTGTTAAAAGACCCGAGTCTG
CTGCGTCAGCAATGTTATAT

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSAIPHTGSYYAASANDKVERARLQESIEADVCVIGAGYTGLSAALHLLESGFSVVVLEAARIGWGASGRNGGQIVNSYS
RDIDTIEKTVGKEQAKLFGQMAFEGGRIIRERIAKYNIDCDLKDGGVFAAMNEKQMGHLRHQKQLWESHGHVGKLELLDA
NSIRSVVNTERYVGGMLDKSGGHIHPLNLALGEARAVESLGGKIFEDSAVLRVDEGDSPVVHTAKGSVKAKFVVVAGNAY
LGKLMPELQAKSMPCGTQVITTEPLDEELAASLLPQDYCVEDCNYLLDYFRLSGDKRLIYGGGVVYGARDPADIESLIIP
NMLKTFPQLKGVKVDYAWTGNFLLTLSRLPQVGRIGKNIYYSQGCSGHGVTYTHLAGKLIAEMLNGQATRFDAFAALPHY
PFPGGHALRVPFSALGAWYYSLRDKLGI

Sequences:

>Translated_428_residues
MSAIPHTGSYYAASANDKVERARLQESIEADVCVIGAGYTGLSAALHLLESGFSVVVLEAARIGWGASGRNGGQIVNSYS
RDIDTIEKTVGKEQAKLFGQMAFEGGRIIRERIAKYNIDCDLKDGGVFAAMNEKQMGHLRHQKQLWESHGHVGKLELLDA
NSIRSVVNTERYVGGMLDKSGGHIHPLNLALGEARAVESLGGKIFEDSAVLRVDEGDSPVVHTAKGSVKAKFVVVAGNAY
LGKLMPELQAKSMPCGTQVITTEPLDEELAASLLPQDYCVEDCNYLLDYFRLSGDKRLIYGGGVVYGARDPADIESLIIP
NMLKTFPQLKGVKVDYAWTGNFLLTLSRLPQVGRIGKNIYYSQGCSGHGVTYTHLAGKLIAEMLNGQATRFDAFAALPHY
PFPGGHALRVPFSALGAWYYSLRDKLGI
>Mature_427_residues
SAIPHTGSYYAASANDKVERARLQESIEADVCVIGAGYTGLSAALHLLESGFSVVVLEAARIGWGASGRNGGQIVNSYSR
DIDTIEKTVGKEQAKLFGQMAFEGGRIIRERIAKYNIDCDLKDGGVFAAMNEKQMGHLRHQKQLWESHGHVGKLELLDAN
SIRSVVNTERYVGGMLDKSGGHIHPLNLALGEARAVESLGGKIFEDSAVLRVDEGDSPVVHTAKGSVKAKFVVVAGNAYL
GKLMPELQAKSMPCGTQVITTEPLDEELAASLLPQDYCVEDCNYLLDYFRLSGDKRLIYGGGVVYGARDPADIESLIIPN
MLKTFPQLKGVKVDYAWTGNFLLTLSRLPQVGRIGKNIYYSQGCSGHGVTYTHLAGKLIAEMLNGQATRFDAFAALPHYP
FPGGHALRVPFSALGAWYYSLRDKLGI

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=424, Percent_Identity=67.9245283018868, Blast_Score=605, Evalue=1e-174,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 46412; Mature: 46280

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIPHTGSYYAASANDKVERARLQESIEADVCVIGAGYTGLSAALHLLESGFSVVVLEA
CCCCCCCCCEEEECCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCEEEEEEE
ARIGWGASGRNGGQIVNSYSRDIDTIEKTVGKEQAKLFGQMAFEGGRIIRERIAKYNIDC
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHCCCCE
DLKDGGVFAAMNEKQMGHLRHQKQLWESHGHVGKLELLDANSIRSVVNTERYVGGMLDKS
EECCCCEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCC
GGHIHPLNLALGEARAVESLGGKIFEDSAVLRVDEGDSPVVHTAKGSVKAKFVVVAGNAY
CCEEEEEHEEHHHHHHHHHHCCEEECCCEEEEECCCCCCEEEECCCCCEEEEEEEECCHH
LGKLMPELQAKSMPCGTQVITTEPLDEELAASLLPQDYCVEDCNYLLDYFRLSGDKRLIY
HHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEE
GGGVVYGARDPADIESLIIPNMLKTFPQLKGVKVDYAWTGNFLLTLSRLPQVGRIGKNIY
ECEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEHHCCCHHHHCCCEE
YSQGCSGHGVTYTHLAGKLIAEMLNGQATRFDAFAALPHYPFPGGHALRVPFSALGAWYY
ECCCCCCCCEEHHHHHHHHHHHHHCCCCCEEHHHHCCCCCCCCCCCEEECCHHHHHHHHH
SLRDKLGI
HHHHHCCC
>Mature Secondary Structure 
SAIPHTGSYYAASANDKVERARLQESIEADVCVIGAGYTGLSAALHLLESGFSVVVLEA
CCCCCCCCEEEECCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCEEEEEEE
ARIGWGASGRNGGQIVNSYSRDIDTIEKTVGKEQAKLFGQMAFEGGRIIRERIAKYNIDC
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHCCCCE
DLKDGGVFAAMNEKQMGHLRHQKQLWESHGHVGKLELLDANSIRSVVNTERYVGGMLDKS
EECCCCEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCC
GGHIHPLNLALGEARAVESLGGKIFEDSAVLRVDEGDSPVVHTAKGSVKAKFVVVAGNAY
CCEEEEEHEEHHHHHHHHHHCCEEECCCEEEEECCCCCCEEEECCCCCEEEEEEEECCHH
LGKLMPELQAKSMPCGTQVITTEPLDEELAASLLPQDYCVEDCNYLLDYFRLSGDKRLIY
HHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEE
GGGVVYGARDPADIESLIIPNMLKTFPQLKGVKVDYAWTGNFLLTLSRLPQVGRIGKNIY
ECEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEHHCCCHHHHCCCEE
YSQGCSGHGVTYTHLAGKLIAEMLNGQATRFDAFAALPHYPFPGGHALRVPFSALGAWYY
ECCCCCCCCEEHHHHHHHHHHHHHCCCCCEEHHHHCCCCCCCCCCCEEECCHHHHHHHHH
SLRDKLGI
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]