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The map label for this gene is potI [H]

Identifier: 117921532

GI number: 117921532

Start: 3712027

End: 3712842

Strand: Reverse

Name: potI [H]

Synonym: Shewana3_3094

Alternate gene names: 117921532

Gene position: 3712842-3712027 (Counterclockwise)

Preceding gene: 117921533

Following gene: 117921531

Centisome position: 74.67

GC content: 50.25

Gene sequence:

>816_bases
ATGAAAAAGCTGAGTTTTTCTTCGGTGATGCTGTGGTTCGGCTTGTTCTTCTTGTACGCACCTATGCTGATCCTAGTGAT
TTATTCGTTCAACGAATCTAAGTTAGTGACAGTGTGGGGCGGGTTTTCACCTAAATGGTACGGCGAGTTATTTGCCGATC
AGCAAATTCTCGATGCGGTGTGGACCAGCCTTCGGATTGCGTTTTACAGCTCAACCATGGCGGTGATTATCGGCACTATG
GCCGCCTTTGTGATGACGCGGTTTAAGCGCTCCTGGGCCAAGCTCACTTTGTCGAACATGATCACTGCGCCTTTGGTGAT
GCCTGAGGTGATCACGGGTCTGTCACTGCTGCTGCTATTTGTGCATATGGCGGATCTCTTAGGTTGGCCGAAAGAGCGCG
GCATGGTGACAGTATGGATTGCCCATTCAACCTTCTGTGCGGCCTATGTGGCTGTGGTGGTGTCGTCAAGATTGCGTGAG
TTAGACATGTCAATCGAAGAGGCGGCAATGGATTTAGGCGCTACGCCGCTTAAAACCTTCTTCCTGATCACTGTGCCCAT
GATTTCACCGGCATTAGTGGCGGGCTGGTTGTTATCCTTTAGTCTGTCACTCGATGACTTAGTGATCGCAAGCTTTGCCT
CAGGCCCAGGTGCAACCACCTTACCTATGGTGGTGTTTTCCTCGGTGCGCTTGGGGGTATCGCCTAAGATCAACGCCTTG
GCAACACTTATCATTCTATGTGTGTCGTTAATCGCTTTCTTGTCTTGGTATATGGCAAGACGCGCCGAAAAACGTGAGCG
TATGCCGTTGAACTAA

Upstream 100 bases:

>100_bases
GGCCAGTGGCCTCATCACTGGCGATTGTGATGCTGGCACTCTTGATTATTCCTATTACCTTATTCCATCGCTATCAGGCG
CGTAGTTTGGAGAAAGACCT

Downstream 100 bases:

>100_bases
TACGCTAGGATGCAGTTATTAGTGGTAAGCACGTAGTCAAAAGTGCTTAGCGACAAAAGGATGGCCTGATTGCCAAGGTC
ATCCTTCTTTCTCCCTCCAA

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MKKLSFSSVMLWFGLFFLYAPMLILVIYSFNESKLVTVWGGFSPKWYGELFADQQILDAVWTSLRIAFYSSTMAVIIGTM
AAFVMTRFKRSWAKLTLSNMITAPLVMPEVITGLSLLLLFVHMADLLGWPKERGMVTVWIAHSTFCAAYVAVVVSSRLRE
LDMSIEEAAMDLGATPLKTFFLITVPMISPALVAGWLLSFSLSLDDLVIASFASGPGATTLPMVVFSSVRLGVSPKINAL
ATLIILCVSLIAFLSWYMARRAEKRERMPLN

Sequences:

>Translated_271_residues
MKKLSFSSVMLWFGLFFLYAPMLILVIYSFNESKLVTVWGGFSPKWYGELFADQQILDAVWTSLRIAFYSSTMAVIIGTM
AAFVMTRFKRSWAKLTLSNMITAPLVMPEVITGLSLLLLFVHMADLLGWPKERGMVTVWIAHSTFCAAYVAVVVSSRLRE
LDMSIEEAAMDLGATPLKTFFLITVPMISPALVAGWLLSFSLSLDDLVIASFASGPGATTLPMVVFSSVRLGVSPKINAL
ATLIILCVSLIAFLSWYMARRAEKRERMPLN
>Mature_271_residues
MKKLSFSSVMLWFGLFFLYAPMLILVIYSFNESKLVTVWGGFSPKWYGELFADQQILDAVWTSLRIAFYSSTMAVIIGTM
AAFVMTRFKRSWAKLTLSNMITAPLVMPEVITGLSLLLLFVHMADLLGWPKERGMVTVWIAHSTFCAAYVAVVVSSRLRE
LDMSIEEAAMDLGATPLKTFFLITVPMISPALVAGWLLSFSLSLDDLVIASFASGPGATTLPMVVFSSVRLGVSPKINAL
ATLIILCVSLIAFLSWYMARRAEKRERMPLN

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=258, Percent_Identity=67.0542635658915, Blast_Score=318, Evalue=2e-88,
Organism=Escherichia coli, GI1787368, Length=238, Percent_Identity=38.2352941176471, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1787714, Length=264, Percent_Identity=31.4393939393939, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1788764, Length=175, Percent_Identity=29.7142857142857, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30093; Mature: 30093

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.9 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLSFSSVMLWFGLFFLYAPMLILVIYSFNESKLVTVWGGFSPKWYGELFADQQILDAV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
WTSLRIAFYSSTMAVIIGTMAAFVMTRFKRSWAKLTLSNMITAPLVMPEVITGLSLLLLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VHMADLLGWPKERGMVTVWIAHSTFCAAYVAVVVSSRLRELDMSIEEAAMDLGATPLKTF
HHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHH
FLITVPMISPALVAGWLLSFSLSLDDLVIASFASGPGATTLPMVVFSSVRLGVSPKINAL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHH
ATLIILCVSLIAFLSWYMARRAEKRERMPLN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKKLSFSSVMLWFGLFFLYAPMLILVIYSFNESKLVTVWGGFSPKWYGELFADQQILDAV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
WTSLRIAFYSSTMAVIIGTMAAFVMTRFKRSWAKLTLSNMITAPLVMPEVITGLSLLLLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VHMADLLGWPKERGMVTVWIAHSTFCAAYVAVVVSSRLRELDMSIEEAAMDLGATPLKTF
HHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHH
FLITVPMISPALVAGWLLSFSLSLDDLVIASFASGPGATTLPMVVFSSVRLGVSPKINAL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHH
ATLIILCVSLIAFLSWYMARRAEKRERMPLN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]