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The map label for this gene is hemL [H]

Identifier: 117921509

GI number: 117921509

Start: 3683258

End: 3684550

Strand: Direct

Name: hemL [H]

Synonym: Shewana3_3071

Alternate gene names: 117921509

Gene position: 3683258-3684550 (Clockwise)

Preceding gene: 117921508

Following gene: 117921511

Centisome position: 74.08

GC content: 50.19

Gene sequence:

>1293_bases
ATGACCCGTTCCGAAGCGCTATTTGAACAGGCTAAAAAAACCATCCCCGGCGGTGTTAACTCTCCGGTTCGTGCATTTAA
CGGTGTAGGTGGTTCGCCGCTGTTTATTGAAAAAGCTGATGGCGCTTATATTTACGATGCCGATGGTAAAGCGTATATCG
ACTATGTTGGCTCTTGGGGCCCGATGATCCTCGGCCACAACCATCCAAAGATCCGTGAAGCCGTATTAGCTGCGGTGCAC
AATGGTCTGTCTTTTGGTGCACCTACCGAGCTTGAAGTACAAATGGCTGAGAAAGTCATCGCCATGGTACCTTCTATAGA
ACAAGTACGTATGGTGAGTTCAGGCACTGAAGCCACCATGAGTGCGATTCGTTTAGCTCGCGGTTTTACCAATCGTGACA
AGATCTTAAAGTTTGAAGGCTGCTACCACGGTCACGCAGACTGCCTATTAGTTAAAGCAGGTTCTGGTGCTTTGACCTTA
GGCCAACCAAGCTCTCCAGGTATTCCTGAAGATTTCGCCAAGCACACCTTAACCGCTGTGTATAACGATCTCGATTCAGT
TCGTAGCCTGTTTGAGCAATATCCAACTGAAATCTCTTGTATCATCATCGAGCCTGTCGCTGGCAACATGAACTGTATTC
CACCTATCCCAGGCTTCCTCGAAGGTCTGCGTGCTATGTGTGATGAGTTTGGTGCGCTACTCATTATCGACGAAGTGATG
ACAGGTTTCCGTGTATCAAGAAGCGGTGCTCAAGGCCACTATGGCGTTACTCCTGACTTAACCACACTGGGTAAAGTGAT
TGGCGGCGGCATGCCTGTGGGTGCATTTGGCGGTCGTAAAGAGGTGATGCAGTTTATCGCTCCAACGGGTCCTGTGTACC
AAGCGGGTACCCTGTCTGGTAACCCCATTGCGATGTCAGCGGGTCTAGCGCAAATGGAAGCCCTGTGTGAAGAAGGTCTA
TACGAAGCGCTAAGCGCGAAAACCAAACGTATCGCCGAAGGCTTTAAAGCGGCGGCCGACAAACACGGTATTCCAATGGC
CATCAACTATGTCGGTGGCATGTTCGGCTTCTTCTTTACCGAGCAAGAGCAGATCACGCGCTTCGACCAAGTGACTAAGT
GCAATATCGAACACTTCCGCACTTTCTATCACGGCATGTTAGATGAAGGGGTTTACTTAGCACCGAGCGCCTATGAAGCA
GGCTTCCTGTCGATGGCCCATGGCGAAGAAGAACTGCGCCTCACGCTAGAAGCGGCTGACCGTGTATTAGGTCGTATGAA
AGCGGCAATGTAA

Upstream 100 bases:

>100_bases
ACTCGGGGTTGACTCGCAACTCGAAAAATATGAGTGTCCCGTTAATTGGTATTCACGTAAAGCCGATCGCTAAGTCGGCT
ACTTACTTTAAGGACTTAAC

Downstream 100 bases:

>100_bases
GCGATAGCTTAAAACACTAAGGGACGCATTTGCGTCCCTTTTTATTGCTTGGCGTTTGAACTGGCTATTGCGTGAGCCTG
ACTCCATCAATAGCTATCGG

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MTRSEALFEQAKKTIPGGVNSPVRAFNGVGGSPLFIEKADGAYIYDADGKAYIDYVGSWGPMILGHNHPKIREAVLAAVH
NGLSFGAPTELEVQMAEKVIAMVPSIEQVRMVSSGTEATMSAIRLARGFTNRDKILKFEGCYHGHADCLLVKAGSGALTL
GQPSSPGIPEDFAKHTLTAVYNDLDSVRSLFEQYPTEISCIIIEPVAGNMNCIPPIPGFLEGLRAMCDEFGALLIIDEVM
TGFRVSRSGAQGHYGVTPDLTTLGKVIGGGMPVGAFGGRKEVMQFIAPTGPVYQAGTLSGNPIAMSAGLAQMEALCEEGL
YEALSAKTKRIAEGFKAAADKHGIPMAINYVGGMFGFFFTEQEQITRFDQVTKCNIEHFRTFYHGMLDEGVYLAPSAYEA
GFLSMAHGEEELRLTLEAADRVLGRMKAAM

Sequences:

>Translated_430_residues
MTRSEALFEQAKKTIPGGVNSPVRAFNGVGGSPLFIEKADGAYIYDADGKAYIDYVGSWGPMILGHNHPKIREAVLAAVH
NGLSFGAPTELEVQMAEKVIAMVPSIEQVRMVSSGTEATMSAIRLARGFTNRDKILKFEGCYHGHADCLLVKAGSGALTL
GQPSSPGIPEDFAKHTLTAVYNDLDSVRSLFEQYPTEISCIIIEPVAGNMNCIPPIPGFLEGLRAMCDEFGALLIIDEVM
TGFRVSRSGAQGHYGVTPDLTTLGKVIGGGMPVGAFGGRKEVMQFIAPTGPVYQAGTLSGNPIAMSAGLAQMEALCEEGL
YEALSAKTKRIAEGFKAAADKHGIPMAINYVGGMFGFFFTEQEQITRFDQVTKCNIEHFRTFYHGMLDEGVYLAPSAYEA
GFLSMAHGEEELRLTLEAADRVLGRMKAAM
>Mature_429_residues
TRSEALFEQAKKTIPGGVNSPVRAFNGVGGSPLFIEKADGAYIYDADGKAYIDYVGSWGPMILGHNHPKIREAVLAAVHN
GLSFGAPTELEVQMAEKVIAMVPSIEQVRMVSSGTEATMSAIRLARGFTNRDKILKFEGCYHGHADCLLVKAGSGALTLG
QPSSPGIPEDFAKHTLTAVYNDLDSVRSLFEQYPTEISCIIIEPVAGNMNCIPPIPGFLEGLRAMCDEFGALLIIDEVMT
GFRVSRSGAQGHYGVTPDLTTLGKVIGGGMPVGAFGGRKEVMQFIAPTGPVYQAGTLSGNPIAMSAGLAQMEALCEEGLY
EALSAKTKRIAEGFKAAADKHGIPMAINYVGGMFGFFFTEQEQITRFDQVTKCNIEHFRTFYHGMLDEGVYLAPSAYEAG
FLSMAHGEEELRLTLEAADRVLGRMKAAM

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=301, Percent_Identity=28.9036544850498, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI284507298, Length=144, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=9e-15,
Organism=Escherichia coli, GI1786349, Length=426, Percent_Identity=70.18779342723, Blast_Score=648, Evalue=0.0,
Organism=Escherichia coli, GI1789016, Length=364, Percent_Identity=31.3186813186813, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1788044, Length=338, Percent_Identity=30.4733727810651, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI145693181, Length=345, Percent_Identity=28.9855072463768, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1789759, Length=336, Percent_Identity=29.1666666666667, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1787560, Length=346, Percent_Identity=29.1907514450867, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1786991, Length=333, Percent_Identity=27.6276276276276, Blast_Score=98, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI71992977, Length=463, Percent_Identity=26.7818574514039, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI25144271, Length=299, Percent_Identity=27.4247491638796, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI32564660, Length=337, Percent_Identity=27.5964391691395, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI25144274, Length=147, Percent_Identity=34.0136054421769, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323470, Length=309, Percent_Identity=28.8025889967638, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324432, Length=353, Percent_Identity=25.4957507082153, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21357415, Length=297, Percent_Identity=28.6195286195286, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21356575, Length=343, Percent_Identity=27.9883381924198, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 46172; Mature: 46040

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRSEALFEQAKKTIPGGVNSPVRAFNGVGGSPLFIEKADGAYIYDADGKAYIDYVGSWG
CCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCEEEEECCCCEEEECCCCEEEEEECCCC
PMILGHNHPKIREAVLAAVHNGLSFGAPTELEVQMAEKVIAMVPSIEQVRMVSSGTEATM
CEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCHHHHH
SAIRLARGFTNRDKILKFEGCYHGHADCLLVKAGSGALTLGQPSSPGIPEDFAKHTLTAV
HHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCEEECCCCCCCCCCHHHHHHHHHHH
YNDLDSVRSLFEQYPTEISCIIIEPVAGNMNCIPPIPGFLEGLRAMCDEFGALLIIDEVM
HHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEHHHHH
TGFRVSRSGAQGHYGVTPDLTTLGKVIGGGMPVGAFGGRKEVMQFIAPTGPVYQAGTLSG
HCCEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCC
NPIAMSAGLAQMEALCEEGLYEALSAKTKRIAEGFKAAADKHGIPMAINYVGGMFGFFFT
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCC
EQEQITRFDQVTKCNIEHFRTFYHGMLDEGVYLAPSAYEAGFLSMAHGEEELRLTLEAAD
CHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHCCCHHHEEHHHHHH
RVLGRMKAAM
HHHHHHHHCC
>Mature Secondary Structure 
TRSEALFEQAKKTIPGGVNSPVRAFNGVGGSPLFIEKADGAYIYDADGKAYIDYVGSWG
CCHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCEEEEECCCCEEEECCCCEEEEEECCCC
PMILGHNHPKIREAVLAAVHNGLSFGAPTELEVQMAEKVIAMVPSIEQVRMVSSGTEATM
CEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCHHHHH
SAIRLARGFTNRDKILKFEGCYHGHADCLLVKAGSGALTLGQPSSPGIPEDFAKHTLTAV
HHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCEEECCCCCCCCCCHHHHHHHHHHH
YNDLDSVRSLFEQYPTEISCIIIEPVAGNMNCIPPIPGFLEGLRAMCDEFGALLIIDEVM
HHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEHHHHH
TGFRVSRSGAQGHYGVTPDLTTLGKVIGGGMPVGAFGGRKEVMQFIAPTGPVYQAGTLSG
HCCEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCC
NPIAMSAGLAQMEALCEEGLYEALSAKTKRIAEGFKAAADKHGIPMAINYVGGMFGFFFT
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCC
EQEQITRFDQVTKCNIEHFRTFYHGMLDEGVYLAPSAYEAGFLSMAHGEEELRLTLEAAD
CHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHCCCHHHEEHHHHHH
RVLGRMKAAM
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA