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The map label for this gene is pyrB [H]

Identifier: 117921508

GI number: 117921508

Start: 3682211

End: 3683230

Strand: Direct

Name: pyrB [H]

Synonym: Shewana3_3070

Alternate gene names: 117921508

Gene position: 3682211-3683230 (Clockwise)

Preceding gene: 117921507

Following gene: 117921509

Centisome position: 74.06

GC content: 48.04

Gene sequence:

>1020_bases
ATGAACCAGTTCGAAGGATCTCATATCCTCTCCGTAAACCAGTTAGATCTTGATTCAATCCAAACTATTTTTAATGTTGC
CCATCGTATGATGCCCTATGCACTTCGTGAGAAGCGCACTAAGGTGCTCGAAGGCGCCATTTTAGGTAACCTGTTTTTCG
AGCCTAGTACCCGTACCCGCGTGAGTTTTGGCTGTGCCTTTAACTTACTCGGCGGCCATGTGCGTGAGACCACAGGCATG
GCCTCTTCGTCCTTATCTAAGGGTGAGTCACTGTACGATACCGCACGTGTACTCTCGACCTATTCCGATGTAATTGCGAT
GCGCCACCCCGACTCTTATTCGGTGAAAGAGTTTGCTGAAGGTAGCCGCGTGCCAGTAATCAACGGTGGTGATGGTTCGA
ACGAGCACCCGACCCAAGCCCTGCTCGATTTGTTCACCATTCAAAAAGAGTTAGGCCATGCTGGCCGCGGTATCGATGGC
ATGCATATCGCTATGGTCGGCGACTTAAAATTTGGTCGTACCGTGCATTCCCTGTCGCGCTTGCTGTGCATGTACAAGAA
CATCAGCTTCACACTGATTTCGCCAACTGAATTAGCAATGCCTGACTATGTGATCTCCGACATTGAAAATGCTGGCCATA
GCATCAAAATAACAGACCAGCTTGAAGGCCACTTAGATAAAGCCGACATACTTTATCTGACCCGCATTCAAGAAGAGCGC
TTCCCATCCCAGGAAGAAGCGAACAAATACCGCGGTAAATTCCGTTTGAATCGCAGTATTTATACCCAACATTGCAAATC
TAACACTGTGATCATGCACCCGCTGCCACGGGATTCGCGTGCGCAAGCTAATGAGTTAGATAATGATTTGAACAGCCATC
CTAATCTCGCTATCTTCAGACAGGCGGACAATGGACTGCTAATTCGTATGGCACTGTTTGCATTGACACTCGGGGTTGAC
TCGCAACTCGAAAAATATGAGTGTCCCGTTAATTGGTATTCACGTAAAGCCGATCGCTAA

Upstream 100 bases:

>100_bases
TGCATCTGTTACAATCGCGCCTCAGCCACCCTACATATACACCAGGGCACAAAGCGCGACATTGCACTTTAACGCCCCCT
AGCAATTGGAAAACAGGCTG

Downstream 100 bases:

>100_bases
GTCGGCTACTTACTTTAAGGACTTAACATGACCCGTTCCGAAGCGCTATTTGAACAGGCTAAAAAAACCATCCCCGGCGG
TGTTAACTCTCCGGTTCGTG

Product: aspartate carbamoyltransferase

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MNQFEGSHILSVNQLDLDSIQTIFNVAHRMMPYALREKRTKVLEGAILGNLFFEPSTRTRVSFGCAFNLLGGHVRETTGM
ASSSLSKGESLYDTARVLSTYSDVIAMRHPDSYSVKEFAEGSRVPVINGGDGSNEHPTQALLDLFTIQKELGHAGRGIDG
MHIAMVGDLKFGRTVHSLSRLLCMYKNISFTLISPTELAMPDYVISDIENAGHSIKITDQLEGHLDKADILYLTRIQEER
FPSQEEANKYRGKFRLNRSIYTQHCKSNTVIMHPLPRDSRAQANELDNDLNSHPNLAIFRQADNGLLIRMALFALTLGVD
SQLEKYECPVNWYSRKADR

Sequences:

>Translated_339_residues
MNQFEGSHILSVNQLDLDSIQTIFNVAHRMMPYALREKRTKVLEGAILGNLFFEPSTRTRVSFGCAFNLLGGHVRETTGM
ASSSLSKGESLYDTARVLSTYSDVIAMRHPDSYSVKEFAEGSRVPVINGGDGSNEHPTQALLDLFTIQKELGHAGRGIDG
MHIAMVGDLKFGRTVHSLSRLLCMYKNISFTLISPTELAMPDYVISDIENAGHSIKITDQLEGHLDKADILYLTRIQEER
FPSQEEANKYRGKFRLNRSIYTQHCKSNTVIMHPLPRDSRAQANELDNDLNSHPNLAIFRQADNGLLIRMALFALTLGVD
SQLEKYECPVNWYSRKADR
>Mature_339_residues
MNQFEGSHILSVNQLDLDSIQTIFNVAHRMMPYALREKRTKVLEGAILGNLFFEPSTRTRVSFGCAFNLLGGHVRETTGM
ASSSLSKGESLYDTARVLSTYSDVIAMRHPDSYSVKEFAEGSRVPVINGGDGSNEHPTQALLDLFTIQKELGHAGRGIDG
MHIAMVGDLKFGRTVHSLSRLLCMYKNISFTLISPTELAMPDYVISDIENAGHSIKITDQLEGHLDKADILYLTRIQEER
FPSQEEANKYRGKFRLNRSIYTQHCKSNTVIMHPLPRDSRAQANELDNDLNSHPNLAIFRQADNGLLIRMALFALTLGVD
SQLEKYECPVNWYSRKADR

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=318, Percent_Identity=44.3396226415094, Blast_Score=256, Evalue=2e-68,
Organism=Homo sapiens, GI38788445, Length=343, Percent_Identity=25.9475218658892, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI2367364, Length=321, Percent_Identity=40.1869158878505, Blast_Score=228, Evalue=4e-61,
Organism=Escherichia coli, GI1786469, Length=343, Percent_Identity=24.4897959183673, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790703, Length=239, Percent_Identity=28.4518828451883, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=330, Percent_Identity=40, Blast_Score=219, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6322331, Length=323, Percent_Identity=43.0340557275542, Blast_Score=249, Evalue=5e-67,
Organism=Saccharomyces cerevisiae, GI6322373, Length=281, Percent_Identity=26.6903914590747, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24642586, Length=314, Percent_Identity=42.0382165605096, Blast_Score=240, Evalue=8e-64,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 38097; Mature: 38097

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQFEGSHILSVNQLDLDSIQTIFNVAHRMMPYALREKRTKVLEGAILGNLFFEPSTRTR
CCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCE
VSFGCAFNLLGGHVRETTGMASSSLSKGESLYDTARVLSTYSDVIAMRHPDSYSVKEFAE
EEECEEEHHHCCHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHC
GSRVPVINGGDGSNEHPTQALLDLFTIQKELGHAGRGIDGMHIAMVGDLKFGRTVHSLSR
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHH
LLCMYKNISFTLISPTELAMPDYVISDIENAGHSIKITDQLEGHLDKADILYLTRIQEER
HHHHHCCCEEEEECCHHHCCCHHHHHHHHHCCCEEEEECHHCCCCCCHHEEEEEHHHHHH
FPSQEEANKYRGKFRLNRSIYTQHCKSNTVIMHPLPRDSRAQANELDNDLNSHPNLAIFR
CCCHHHHHHHCCCEEECHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCEEEEE
QADNGLLIRMALFALTLGVDSQLEKYECPVNWYSRKADR
ECCCCCHHHHHHHHHHHCCCCHHHHHCCCCHHHHCCCCC
>Mature Secondary Structure
MNQFEGSHILSVNQLDLDSIQTIFNVAHRMMPYALREKRTKVLEGAILGNLFFEPSTRTR
CCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCE
VSFGCAFNLLGGHVRETTGMASSSLSKGESLYDTARVLSTYSDVIAMRHPDSYSVKEFAE
EEECEEEHHHCCHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHC
GSRVPVINGGDGSNEHPTQALLDLFTIQKELGHAGRGIDGMHIAMVGDLKFGRTVHSLSR
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHH
LLCMYKNISFTLISPTELAMPDYVISDIENAGHSIKITDQLEGHLDKADILYLTRIQEER
HHHHHCCCEEEEECCHHHCCCHHHHHHHHHCCCEEEEECHHCCCCCCHHEEEEEHHHHHH
FPSQEEANKYRGKFRLNRSIYTQHCKSNTVIMHPLPRDSRAQANELDNDLNSHPNLAIFR
CCCHHHHHHHCCCEEECHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCEEEEE
QADNGLLIRMALFALTLGVDSQLEKYECPVNWYSRKADR
ECCCCCHHHHHHHHHHHCCCCHHHHHCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA