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The map label for this gene is cobD [H]

Identifier: 117921491

GI number: 117921491

Start: 3661068

End: 3662057

Strand: Direct

Name: cobD [H]

Synonym: Shewana3_3053

Alternate gene names: 117921491

Gene position: 3661068-3662057 (Clockwise)

Preceding gene: 117921490

Following gene: 117921492

Centisome position: 73.63

GC content: 50.71

Gene sequence:

>990_bases
ATGCACAGTTCTTTTTATCAACAACTTGTCGAAATTGATGGCGCTTTGTTTGAGGGCTTCTTGGTACTTTTCTTTGCTTT
ATTGCTGGCGCGTTTAGCGCCATTGCCAAGGGAAATGCAGCCGCTAATTTGGTTTAGTCACTTAGCCAAACAGCTCGCCG
CCAAAGTCAATCGCCCTGGGCGCGCGCCCAGCCAACAGGCAACCGCCGGCTTTTTAGCCATGTTGCTGCTAGTGTTTCCC
TTCTGGGCAATTATCACCTTTTTACTCGAGCTCGCCGCTTTCCCTTGGTTCTTCGAATTTTTAGTTCTGTATCTGTGTTT
GACGGATGCAGGCTTTAGCCAAGTCGCCGACGAAATCGCTAAAGCATTGAAGCGCGAGGACAATGCCAGCGCAAAAAAGC
TATTACAGCCGTGGATTGTCCGTGATACGGATAATCTATCGGAAGTCGGCTTAACCAAGGCCACGATAGAAAAGCTCGCT
ACCGCGCCGATTTATGGCACGGCATCGACCATCTTCTTCTTTGCGATCGCAGGCGCCCCTATGGTGCTGGCGGTCAGAAT
GATTAAACAGCTCGAACTCAGTTGGCCGCCCATTCAACCTAAGTACCAGCACTTTTGCAGTTCGGTGCACGTTATTTCTA
CGACGCTGCTGGCAATCCCCGCTTGGTTATGGAGCCTGTCGATTGCGATTCAAGGCGGCCCCAAGGCCTTAGCATTGCTG
TTTCGCACCTCTAAAAATCATCCAGCCCTGCGTGGTTATGTGATGAGTGTGTCCTTAGTGGCGAATATTTTACGCATTGA
ATTGGGTGGACCCCAGAAGTTTTCGGGGCAACGCATTGATGTGGCTAAGATAGTCTATGGCCCACAGCCGCACCAAGAGA
TGATTGCCCCCGCAGTAAAACTCGCCTCACGGGCCTGCGCTATCTGGTTTAGCTTTATTACCTTACTTCCGATCATTTGG
GCGGGTTTACGCTGGCTACAAACCCTGTGA

Upstream 100 bases:

>100_bases
GCAGGCTACCATTCGGCACTGATGGTGATGTTGCTGCTTGAGCAATTAGATCCAAATTCAGTAAAGTAATTGACTAAAGC
ATGATTGCGGAAAGTACCTG

Downstream 100 bases:

>100_bases
TATGATGCCTTTAATCTTAACATTAGGTTAAAGGCTGACTATCTTGTTCGAAAACATGCATGTTTCATTAACAACTCCCG
CCTTGCTATTTCCGGCAATC

Product: cobalamin biosynthesis protein CbiB

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MHSSFYQQLVEIDGALFEGFLVLFFALLLARLAPLPREMQPLIWFSHLAKQLAAKVNRPGRAPSQQATAGFLAMLLLVFP
FWAIITFLLELAAFPWFFEFLVLYLCLTDAGFSQVADEIAKALKREDNASAKKLLQPWIVRDTDNLSEVGLTKATIEKLA
TAPIYGTASTIFFFAIAGAPMVLAVRMIKQLELSWPPIQPKYQHFCSSVHVISTTLLAIPAWLWSLSIAIQGGPKALALL
FRTSKNHPALRGYVMSVSLVANILRIELGGPQKFSGQRIDVAKIVYGPQPHQEMIAPAVKLASRACAIWFSFITLLPIIW
AGLRWLQTL

Sequences:

>Translated_329_residues
MHSSFYQQLVEIDGALFEGFLVLFFALLLARLAPLPREMQPLIWFSHLAKQLAAKVNRPGRAPSQQATAGFLAMLLLVFP
FWAIITFLLELAAFPWFFEFLVLYLCLTDAGFSQVADEIAKALKREDNASAKKLLQPWIVRDTDNLSEVGLTKATIEKLA
TAPIYGTASTIFFFAIAGAPMVLAVRMIKQLELSWPPIQPKYQHFCSSVHVISTTLLAIPAWLWSLSIAIQGGPKALALL
FRTSKNHPALRGYVMSVSLVANILRIELGGPQKFSGQRIDVAKIVYGPQPHQEMIAPAVKLASRACAIWFSFITLLPIIW
AGLRWLQTL
>Mature_329_residues
MHSSFYQQLVEIDGALFEGFLVLFFALLLARLAPLPREMQPLIWFSHLAKQLAAKVNRPGRAPSQQATAGFLAMLLLVFP
FWAIITFLLELAAFPWFFEFLVLYLCLTDAGFSQVADEIAKALKREDNASAKKLLQPWIVRDTDNLSEVGLTKATIEKLA
TAPIYGTASTIFFFAIAGAPMVLAVRMIKQLELSWPPIQPKYQHFCSSVHVISTTLLAIPAWLWSLSIAIQGGPKALALL
FRTSKNHPALRGYVMSVSLVANILRIELGGPQKFSGQRIDVAKIVYGPQPHQEMIAPAVKLASRACAIWFSFITLLPIIW
AGLRWLQTL

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 36605; Mature: 36605

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSSFYQQLVEIDGALFEGFLVLFFALLLARLAPLPREMQPLIWFSHLAKQLAAKVNRPG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCC
RAPSQQATAGFLAMLLLVFPFWAIITFLLELAAFPWFFEFLVLYLCLTDAGFSQVADEIA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KALKREDNASAKKLLQPWIVRDTDNLSEVGLTKATIEKLATAPIYGTASTIFFFAIAGAP
HHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCH
MVLAVRMIKQLELSWPPIQPKYQHFCSSVHVISTTLLAIPAWLWSLSIAIQGGPKALALL
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
FRTSKNHPALRGYVMSVSLVANILRIELGGPQKFSGQRIDVAKIVYGPQPHQEMIAPAVK
HHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHEECCCCHHHHHHHHHH
LASRACAIWFSFITLLPIIWAGLRWLQTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHSSFYQQLVEIDGALFEGFLVLFFALLLARLAPLPREMQPLIWFSHLAKQLAAKVNRPG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCC
RAPSQQATAGFLAMLLLVFPFWAIITFLLELAAFPWFFEFLVLYLCLTDAGFSQVADEIA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KALKREDNASAKKLLQPWIVRDTDNLSEVGLTKATIEKLATAPIYGTASTIFFFAIAGAP
HHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCH
MVLAVRMIKQLELSWPPIQPKYQHFCSSVHVISTTLLAIPAWLWSLSIAIQGGPKALALL
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
FRTSKNHPALRGYVMSVSLVANILRIELGGPQKFSGQRIDVAKIVYGPQPHQEMIAPAVK
HHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHEECCCCHHHHHHHHHH
LASRACAIWFSFITLLPIIWAGLRWLQTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 14500782 [H]