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The map label for this gene is pdxJ [H]

Identifier: 117921462

GI number: 117921462

Start: 3628834

End: 3629571

Strand: Reverse

Name: pdxJ [H]

Synonym: Shewana3_3023

Alternate gene names: 117921462

Gene position: 3629571-3628834 (Counterclockwise)

Preceding gene: 117921463

Following gene: 117921461

Centisome position: 73.0

GC content: 51.63

Gene sequence:

>738_bases
ATGAGCCGTATCTTATTGGGTGTTAATATCGACCATATTGCGACCCTGCGTCAGGCTCGCGGCACCAGTTATCCTGATCC
TGTCCATGCAGCGGCGGTTGCCGAGCATGCGGGTGCCGACGGTATCACGATTCATTTACGGGAAGACAGACGTCATATTA
TCGACCGCGATGTGTATCTGCTGGCGAAAACCTTAAAGACGCGGATGAACTTTGAGTGTGCCGTGACAGAAGAAATGCTC
AACATCGCCTGTGAAGTCAAACCGACCTACGTTTGCTTAGTCCCTGAAAAACGTCAAGAAGTGACGACCGAAGGTGGGTT
AGATGTGGCTGGTCAACTAGATAAAATCACTGCCGCGGTAAGCCGTTTAGCCGCCAATCGTATCCAAGTGTCACTGTTTA
TCGATGCCGATAAGACGCAAATCGATGCCGCCGTCGCCGCTGGCGCGCCACTTATCGAAATTCATACCGGTTGCTATGCC
GACGCTAAAACCGCTGAAGAAGAAGCCAAAGAGCTTGCACGTATCAGCGAAATGGCAAAATACGCCCATGGCAAAGGCTT
AGTCGTGAATGCGGGCCACGGGCTGCATTACCACAATGTTAAGCCGATTGCGGCGATCCCAGAGCTGTATGAACTCAATA
TTGGCCATGCCATTGTCGCGCGAGCGGCCATTGATGGCTTAGCGACAGCGGTTAAAGACATGAAAGCGCTGATGTTAGAA
GGTCGTCGAGGCGAATAA

Upstream 100 bases:

>100_bases
GGAATAATTAGCCCTAGCTCCTGTTTTTTACTGTCTGTCCCAGTACAATAGGGCGCAAATCAGCGTTAATTCTAGCCTTA
CAAACATAAAGGAGTTCCCG

Downstream 100 bases:

>100_bases
TGGCAATCGTTGGGCTTGGCACTGACATAGTTGAAATCGAGCGTATCACCGCCCATGTGGCACGTTCCGGCGATAAGCTG
GCTAAACGTGTACTGACGGA

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase [H]

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MSRILLGVNIDHIATLRQARGTSYPDPVHAAAVAEHAGADGITIHLREDRRHIIDRDVYLLAKTLKTRMNFECAVTEEML
NIACEVKPTYVCLVPEKRQEVTTEGGLDVAGQLDKITAAVSRLAANRIQVSLFIDADKTQIDAAVAAGAPLIEIHTGCYA
DAKTAEEEAKELARISEMAKYAHGKGLVVNAGHGLHYHNVKPIAAIPELYELNIGHAIVARAAIDGLATAVKDMKALMLE
GRRGE

Sequences:

>Translated_245_residues
MSRILLGVNIDHIATLRQARGTSYPDPVHAAAVAEHAGADGITIHLREDRRHIIDRDVYLLAKTLKTRMNFECAVTEEML
NIACEVKPTYVCLVPEKRQEVTTEGGLDVAGQLDKITAAVSRLAANRIQVSLFIDADKTQIDAAVAAGAPLIEIHTGCYA
DAKTAEEEAKELARISEMAKYAHGKGLVVNAGHGLHYHNVKPIAAIPELYELNIGHAIVARAAIDGLATAVKDMKALMLE
GRRGE
>Mature_244_residues
SRILLGVNIDHIATLRQARGTSYPDPVHAAAVAEHAGADGITIHLREDRRHIIDRDVYLLAKTLKTRMNFECAVTEEMLN
IACEVKPTYVCLVPEKRQEVTTEGGLDVAGQLDKITAAVSRLAANRIQVSLFIDADKTQIDAAVAAGAPLIEIHTGCYAD
AKTAEEEAKELARISEMAKYAHGKGLVVNAGHGLHYHNVKPIAAIPELYELNIGHAIVARAAIDGLATAVKDMKALMLEG
RRGE

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate [H]

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788917, Length=242, Percent_Identity=71.900826446281, Blast_Score=346, Evalue=6e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004569 [H]

Pfam domain/function: PF03740 PdxJ [H]

EC number: =2.6.99.2 [H]

Molecular weight: Translated: 26486; Mature: 26355

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRILLGVNIDHIATLRQARGTSYPDPVHAAAVAEHAGADGITIHLREDRRHIIDRDVYL
CCEEEEECCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHH
LAKTLKTRMNFECAVTEEMLNIACEVKPTYVCLVPEKRQEVTTEGGLDVAGQLDKITAAV
HHHHHHHHCCCEEEEHHHHHHEEEECCCEEEEEECCHHHHHCCCCCCCCCCHHHHHHHHH
SRLAANRIQVSLFIDADKTQIDAAVAAGAPLIEIHTGCYADAKTAEEEAKELARISEMAK
HHHHHCEEEEEEEEECCCHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
YAHGKGLVVNAGHGLHYHNVKPIAAIPELYELNIGHAIVARAAIDGLATAVKDMKALMLE
HHCCCCEEEECCCCEEECCCCCHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GRRGE
CCCCC
>Mature Secondary Structure 
SRILLGVNIDHIATLRQARGTSYPDPVHAAAVAEHAGADGITIHLREDRRHIIDRDVYL
CEEEEECCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHH
LAKTLKTRMNFECAVTEEMLNIACEVKPTYVCLVPEKRQEVTTEGGLDVAGQLDKITAAV
HHHHHHHHCCCEEEEHHHHHHEEEECCCEEEEEECCHHHHHCCCCCCCCCCHHHHHHHHH
SRLAANRIQVSLFIDADKTQIDAAVAAGAPLIEIHTGCYADAKTAEEEAKELARISEMAK
HHHHHCEEEEEEEEECCCHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
YAHGKGLVVNAGHGLHYHNVKPIAAIPELYELNIGHAIVARAAIDGLATAVKDMKALMLE
HHCCCCEEEECCCCEEECCCCCHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GRRGE
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]