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The map label for this gene is yhcK [H]

Identifier: 117921386

GI number: 117921386

Start: 3540394

End: 3542121

Strand: Reverse

Name: yhcK [H]

Synonym: Shewana3_2946

Alternate gene names: 117921386

Gene position: 3542121-3540394 (Counterclockwise)

Preceding gene: 117921387

Following gene: 117921385

Centisome position: 71.24

GC content: 44.85

Gene sequence:

>1728_bases
ATGGCTAACTCTCTCGATTTTGATCAGGCACTCAATGAGCTTGATGTATTACTGCAATCTGATCTTAGCGCCGCGCAGAA
AAAACTGGCCGAATATGAGAAAGAATTCAGCAAATTAACTTCAGTGCAACAAGGCCGACTGAGGATAGATAGAGCGATAG
GTTATTTTTTCACAGGTGAATATGCCAAAGCGTTAGACGATCTGCATCAGGCCGAAACCTTAATCACAAGTTCTGAATTA
TTAGCATCGGTTTATAGCTATCAAGCCACTGTCTATATAGGGATGCGCGACTATGACAAAGCCTTATCGGCTGTCGCCAA
AGCATTAGCCCTAGTTGTGAATATCGATGATATTACGATTAAACGTCACTCTTATCTAAGAATTGCCAATATTTATTCTG
AAATGGACGCTTATAGCGATATGGGCCTCTATGCCAATAAGGTGTTACAACTCGCTCAGGAAACCGATGTCCGCGATATT
TGCCATGCCAAATTGCTGCGGGCGATTTACTTGCTCGGTGTGAAAAGTCCCGACACCTTCGATGCCTTCGAAGATAGCCG
TCAATATTGTGATGCGAAGCAATTTCCCCTTTTGGCGACTATGTCGATGAAAGGCAAAGGGATGGTTTTGCAGGAGCGGG
GTGACTATTTAGGCGCGAGAGCGATTTTGCTATTAGCGTTAAAAGGGTATGAAGCTTTTCAATTTCAATTAGAAATCAGC
CATGTGCATGCACTACTTGCCGAGAATTACTTTGCAACGGGTGAGCTGGTTCAAGCAAAAAGTCACGCTGTTAAGGTGCT
CACATTTCCCGATGATAATAGTTATATCGAACATAAGCATATTGCCTACAAAGTGTTTGCCGAGTTGATGGCGGCGGACA
AACAATTTGAGCAAGCCTATGAATACTCCAGAAAAGAGCAACATTATAATCAACTGATTTTTGACCAATCTAAGATGAAA
ACCCTTGCTTATCAGGCGGCTAAGTTTAATGCCGATGAGCAGGCGCGTGAAATCAACTTGCTGAATAAAGAGCGCGAACT
TTACATCGCTCAGCAAACGGTAAAAGAGCGCGAGTACACTAATATGCTGATGTTTATCACCATATTAGTTGGCGGGCTGT
TTTTCCTCGCGATTTTGTTAGTGGTGGGTAATTTGCAAAAACGGCGTTTTATGCGCATGGCCAGAATGGATGCTTTGACG
GGCGTGCTTAACCGTGGAGCAGGGCAAGATCTGGGGGAAAATCTGTTTGTGCAAGCCGCTGCCCGAGGGGGCGATTATTG
CGTGATTTTATTCGACTTGGACCATTTTAAACGGATTAATGATTCCTACGGCCATGGTACGGGTGACTGGGCGCTTAAAA
AAGTCGTAGAAGTATTAAAACCCCATGTTCGCAATGGTGATGTGTTTGCCCGGATTGGCGGTGAGGAATTTGCACTATTC
TTGCCTTACGCCAATGAATCTAAGGGGATGGAAGTTGCCGAACAATGCCGTCGACGACTCGAAGCCATTGATACTCATCT
GTCGGGGCATAAATTTACTATCACCGCGAGCTTTGGGGTTAGCGGTATGACAAAAGAAGACTTAAGCCTAGACCCTCTGC
TGCACCGTGCCGATATGGCGCTGTATGCTGCAAAATCCAATGGTCGTAACAGTGTATTCTGTTATGAGGACGCTTTAGCC
TCAGATAAACGCGCCACCAATATTACCAGTCAGTTTGCAAGGCAGTAG

Upstream 100 bases:

>100_bases
TCAAACCTTTGTTTTAGTTAATCACGGTAATAATTAACTTATCTTGAAACGGTTGCGTTGTGCCCTATTAGCCATCTTTG
CTTTGTGTTCGACAGCGTTG

Downstream 100 bases:

>100_bases
GGGGAGGAGTTGTGTTTTCTGTGTAACTGACTGTTTAGGCTAATGTAACGCGGTTTTCTTCTTTCGCTGTTTCGCTTGTT
TCTTGCGTTTTTTCGATGAT

Product: diguanylate cyclase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 575; Mature: 574

Protein sequence:

>575_residues
MANSLDFDQALNELDVLLQSDLSAAQKKLAEYEKEFSKLTSVQQGRLRIDRAIGYFFTGEYAKALDDLHQAETLITSSEL
LASVYSYQATVYIGMRDYDKALSAVAKALALVVNIDDITIKRHSYLRIANIYSEMDAYSDMGLYANKVLQLAQETDVRDI
CHAKLLRAIYLLGVKSPDTFDAFEDSRQYCDAKQFPLLATMSMKGKGMVLQERGDYLGARAILLLALKGYEAFQFQLEIS
HVHALLAENYFATGELVQAKSHAVKVLTFPDDNSYIEHKHIAYKVFAELMAADKQFEQAYEYSRKEQHYNQLIFDQSKMK
TLAYQAAKFNADEQAREINLLNKERELYIAQQTVKEREYTNMLMFITILVGGLFFLAILLVVGNLQKRRFMRMARMDALT
GVLNRGAGQDLGENLFVQAAARGGDYCVILFDLDHFKRINDSYGHGTGDWALKKVVEVLKPHVRNGDVFARIGGEEFALF
LPYANESKGMEVAEQCRRRLEAIDTHLSGHKFTITASFGVSGMTKEDLSLDPLLHRADMALYAAKSNGRNSVFCYEDALA
SDKRATNITSQFARQ

Sequences:

>Translated_575_residues
MANSLDFDQALNELDVLLQSDLSAAQKKLAEYEKEFSKLTSVQQGRLRIDRAIGYFFTGEYAKALDDLHQAETLITSSEL
LASVYSYQATVYIGMRDYDKALSAVAKALALVVNIDDITIKRHSYLRIANIYSEMDAYSDMGLYANKVLQLAQETDVRDI
CHAKLLRAIYLLGVKSPDTFDAFEDSRQYCDAKQFPLLATMSMKGKGMVLQERGDYLGARAILLLALKGYEAFQFQLEIS
HVHALLAENYFATGELVQAKSHAVKVLTFPDDNSYIEHKHIAYKVFAELMAADKQFEQAYEYSRKEQHYNQLIFDQSKMK
TLAYQAAKFNADEQAREINLLNKERELYIAQQTVKEREYTNMLMFITILVGGLFFLAILLVVGNLQKRRFMRMARMDALT
GVLNRGAGQDLGENLFVQAAARGGDYCVILFDLDHFKRINDSYGHGTGDWALKKVVEVLKPHVRNGDVFARIGGEEFALF
LPYANESKGMEVAEQCRRRLEAIDTHLSGHKFTITASFGVSGMTKEDLSLDPLLHRADMALYAAKSNGRNSVFCYEDALA
SDKRATNITSQFARQ
>Mature_574_residues
ANSLDFDQALNELDVLLQSDLSAAQKKLAEYEKEFSKLTSVQQGRLRIDRAIGYFFTGEYAKALDDLHQAETLITSSELL
ASVYSYQATVYIGMRDYDKALSAVAKALALVVNIDDITIKRHSYLRIANIYSEMDAYSDMGLYANKVLQLAQETDVRDIC
HAKLLRAIYLLGVKSPDTFDAFEDSRQYCDAKQFPLLATMSMKGKGMVLQERGDYLGARAILLLALKGYEAFQFQLEISH
VHALLAENYFATGELVQAKSHAVKVLTFPDDNSYIEHKHIAYKVFAELMAADKQFEQAYEYSRKEQHYNQLIFDQSKMKT
LAYQAAKFNADEQAREINLLNKERELYIAQQTVKEREYTNMLMFITILVGGLFFLAILLVVGNLQKRRFMRMARMDALTG
VLNRGAGQDLGENLFVQAAARGGDYCVILFDLDHFKRINDSYGHGTGDWALKKVVEVLKPHVRNGDVFARIGGEEFALFL
PYANESKGMEVAEQCRRRLEAIDTHLSGHKFTITASFGVSGMTKEDLSLDPLLHRADMALYAAKSNGRNSVFCYEDALAS
DKRATNITSQFARQ

Specific function: Unknown

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=163, Percent_Identity=33.7423312883436, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87081881, Length=161, Percent_Identity=39.7515527950311, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI87082007, Length=197, Percent_Identity=33.502538071066, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI145693134, Length=165, Percent_Identity=35.1515151515151, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788085, Length=163, Percent_Identity=31.2883435582822, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1788381, Length=206, Percent_Identity=29.6116504854369, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1786584, Length=168, Percent_Identity=32.7380952380952, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1787802, Length=177, Percent_Identity=35.0282485875706, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI87081977, Length=172, Percent_Identity=33.7209302325581, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787816, Length=165, Percent_Identity=35.7575757575758, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI87081974, Length=198, Percent_Identity=29.2929292929293, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1787541, Length=168, Percent_Identity=29.7619047619048, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1787056, Length=191, Percent_Identity=32.4607329842932, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1788956, Length=164, Percent_Identity=34.7560975609756, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR011620 [H]

Pfam domain/function: PF07694 5TM-5TMR_LYT; PF00990 GGDEF [H]

EC number: NA

Molecular weight: Translated: 64973; Mature: 64842

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS50293 TPR_REGION ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANSLDFDQALNELDVLLQSDLSAAQKKLAEYEKEFSKLTSVQQGRLRIDRAIGYFFTGE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
YAKALDDLHQAETLITSSELLASVYSYQATVYIGMRDYDKALSAVAKALALVVNIDDITI
HHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECHHHHHHHHHHHHHHHHEEECCCEEE
KRHSYLRIANIYSEMDAYSDMGLYANKVLQLAQETDVRDICHAKLLRAIYLLGVKSPDTF
ECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCH
DAFEDSRQYCDAKQFPLLATMSMKGKGMVLQERGDYLGARAILLLALKGYEAFQFQLEIS
HHHHHHHHHCCCCCCCEEEEEECCCCCEEEECCCCHHHHHHHHHHHHCCCEEEEEEEEHH
HVHALLAENYFATGELVQAKSHAVKVLTFPDDNSYIEHKHIAYKVFAELMAADKQFEQAY
HHHHHHHHCCCCCCHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EYSRKEQHYNQLIFDQSKMKTLAYQAAKFNADEQAREINLLNKERELYIAQQTVKEREYT
HHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
NMLMFITILVGGLFFLAILLVVGNLQKRRFMRMARMDALTGVLNRGAGQDLGENLFVQAA
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
ARGGDYCVILFDLDHFKRINDSYGHGTGDWALKKVVEVLKPHVRNGDVFARIGGEEFALF
CCCCCEEEEEEECHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEE
LPYANESKGMEVAEQCRRRLEAIDTHLSGHKFTITASFGVSGMTKEDLSLDPLLHRADMA
EECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHH
LYAAKSNGRNSVFCYEDALASDKRATNITSQFARQ
EEEECCCCCCCEEEEHHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
ANSLDFDQALNELDVLLQSDLSAAQKKLAEYEKEFSKLTSVQQGRLRIDRAIGYFFTGE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
YAKALDDLHQAETLITSSELLASVYSYQATVYIGMRDYDKALSAVAKALALVVNIDDITI
HHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECHHHHHHHHHHHHHHHHEEECCCEEE
KRHSYLRIANIYSEMDAYSDMGLYANKVLQLAQETDVRDICHAKLLRAIYLLGVKSPDTF
ECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCH
DAFEDSRQYCDAKQFPLLATMSMKGKGMVLQERGDYLGARAILLLALKGYEAFQFQLEIS
HHHHHHHHHCCCCCCCEEEEEECCCCCEEEECCCCHHHHHHHHHHHHCCCEEEEEEEEHH
HVHALLAENYFATGELVQAKSHAVKVLTFPDDNSYIEHKHIAYKVFAELMAADKQFEQAY
HHHHHHHHCCCCCCHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EYSRKEQHYNQLIFDQSKMKTLAYQAAKFNADEQAREINLLNKERELYIAQQTVKEREYT
HHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
NMLMFITILVGGLFFLAILLVVGNLQKRRFMRMARMDALTGVLNRGAGQDLGENLFVQAA
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
ARGGDYCVILFDLDHFKRINDSYGHGTGDWALKKVVEVLKPHVRNGDVFARIGGEEFALF
CCCCCEEEEEEECHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEE
LPYANESKGMEVAEQCRRRLEAIDTHLSGHKFTITASFGVSGMTKEDLSLDPLLHRADMA
EECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHH
LYAAKSNGRNSVFCYEDALASDKRATNITSQFARQ
EEEECCCCCCCEEEEHHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969498; 9384377 [H]