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The map label for this gene is glgX [H]

Identifier: 117921373

GI number: 117921373

Start: 3519425

End: 3521683

Strand: Reverse

Name: glgX [H]

Synonym: Shewana3_2933

Alternate gene names: 117921373

Gene position: 3521683-3519425 (Counterclockwise)

Preceding gene: 117921374

Following gene: 117921372

Centisome position: 70.83

GC content: 52.28

Gene sequence:

>2259_bases
ATGACGAATCCTGTGCCTGTAAATGCTGATGGCTCAACCGCGTATAACCTAAGTGCGGGGCAGCCCTTTCCCTTGGGGGC
GACGGTCGATGATGGCGGGGTGAATTTCGCCCTGTTTTCGGCCCATGCCACTGGGGTGGAGTTATGTCTGTTTAATGCCC
GAGGCGAGGTGGAAATTCAGCGCATCGCCTTGACGGAGCAGACCCAGCAGATCTGGCATTTGTATGTGCATGGACTGAGT
GCCGGCCAGCTTTATGGCTACCGTGTTTATGGTCCCTACGAGCCGCAATTAGGCCACAGATTTAATCCGCATAAGTTGCT
ATTAGACCCTTACGCTCGCCAGCTCGTGGGACGCTATCACCACCATATCGCCAACTTTGGTTATGAGATTGATAACCCCA
ACGAGGACTTATCTTTCAGTACCTTAGATAACGCGGACTTTGTCCCAAAATGCAAAGTGGTCGATACTCGACCCCTTTTG
GATGCCGCTAAGGCCCATACTATTCGGCCGCTTGCAAGCAAGCGTGAGCCATTGCCACTTGAGCAGTGCATTATCTATGA
GATGCACTTAAAGGGATTTACCGCGCTGCACCCTGAGATAGATGCGCCGCTGCGTGGTACCTTTGCTGGGCTGGCATCGA
ATGCCGCGATAGATTACTTAGTCAATCTCGGCGTGAATTGCGTCGAGTTACTGCCGGTTCAGGCATTTTTCTCCGAGCCC
TTTCTGCTTGAAAAACAGCTATCTAACTATTGGGGCTATAACAGCATTGGCTTTTTTGCGCCTGAGCCGAGTTATCTCTC
GGGTGAGGATATCGGCGAGTTTCGCAACATGGTGGATGCGCTGCACGGTGCGGGCATCGAAGTCATTCTCGATGTGGTCT
ATAACCACAGCGCCGAGGGCAGTCGTCTCGGACCGACTTTTAGCTTTCGCGGCATCGACAATCTGAGTTATTACCGACTG
CATCCAAACGATAAACGCTTTTATATCAACGATACCGGCTGCGGCAATACCTTAAACCTCAATCACCCACGCATGTTGCA
GTTGGTGTTAGATTCGCTGCGTTACTGGGTCGAAGTGATGGGGGTCGATGGCTTTCGTTTCGATTTAGCCGCCTGCCTTG
GCCGTGAAGCCTATGGATTCGACCCAGGTAGTGGTTTTTTCGATGCGTTGCTGCAAGACCCAGTACTTTGCCGGGTGAAG
CTGATTGCCGAGCCTTGGGATATTGGTCCGGGTGGGTATCAGCTGGGTAATTTCCCGGTGGCCTTTAGCGAGTGGAATGA
CAGATACCGCGACACTATGCGGCGTTTTTGGCGGGGCGATCACGCCATGTTGCCCGAGTTTGCGAGGCGATTTCATGGCT
CGGGGGATTTCTTTGAGCATAGCGGTCGTCCTCCTGCCGCCAGTATCAACTTTTTAACCAGTCACGATGGTTTTACCCTT
AAAGATTTAGTGAGTTATTGCGAGCGGCACAATTGGGCCAACGGTGAAGAAAACCGCGACGGCCACCATGAGAACTTCAG
CCATCACTACGGAGTAGAGGGCGACACCGACGACGCCACCATTCTTGCGCTGCGCGCTCGCCAGCAACGCAATCTATTGA
CCACATTATTCCTTTCCCAAGGGGTGCCCATGTTACTCAGCGGCGATGAGACGGGCCGCACCCAAGGCGGGAATAATAAC
GCCTATTGCCAAGATAACCCATTGAATTGGTTCGATTGGTCATCGGATGGCATGGATAGCTCATTGCTGAGTTTCACCTC
ACAGTTGATTGCCTTGCGTAAACGCTTTCCGCTCTTGTGTGCCAAACGTTTTATCCATGAGCAGCTAGTGGCCAATGCTT
TCGATTGGTCGTCTGAGTCATCTTCAACTCAAGCAGCAAGCCCTGTATCAACCCCATCGGGGGCGAGGCTGGATTGGTTT
AGTCGCCAAGGTGAACCTATGAGCAAAAGCCTGTGGAGTGAGTCCATGGGCCGCAGCCTCTGCGTGGTGCTATCGGGCAA
TTTAACCGCCACTCAACCACAGACTCAGGGTGACAGCCTTCAAGCACTGTTGTTAATGGTCAATGCCGATGAGCATCCCT
TGGCCTTTACTCTGCCGATTTTTGACGGTTTAGGGCCGTGGCAATGTCTGCTGCATACCCAAACCGACCCACAATTTTTA
ACTCCCACTGAGCCGCTGGATGCTCAGTCCTCACCGACGCGGTACTTGCTACAGGATCGCAGTCTTATGCTGTTTCATGC
TGATTTTATAAGGAATTAA

Upstream 100 bases:

>100_bases
TACCTTGGCAGGGCATGGCATCGAGCGCGTTGATCACTGTGCCGCCCCTGGGTTGCTTAATCCTCGGGCCTGCGACGGAT
GCGCCAAGAGATACCAGCCT

Downstream 100 bases:

>100_bases
CTATGAGTCATAACAGTGAGCTGAGCCCAAATACCAGCGGCGTTCCCCCCAAAAAACGTGCCACTAAGGCCGCGCCGAGT
AAGCCAAAAGCGACACTCGA

Product: glycogen debranching enzyme GlgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 752; Mature: 751

Protein sequence:

>752_residues
MTNPVPVNADGSTAYNLSAGQPFPLGATVDDGGVNFALFSAHATGVELCLFNARGEVEIQRIALTEQTQQIWHLYVHGLS
AGQLYGYRVYGPYEPQLGHRFNPHKLLLDPYARQLVGRYHHHIANFGYEIDNPNEDLSFSTLDNADFVPKCKVVDTRPLL
DAAKAHTIRPLASKREPLPLEQCIIYEMHLKGFTALHPEIDAPLRGTFAGLASNAAIDYLVNLGVNCVELLPVQAFFSEP
FLLEKQLSNYWGYNSIGFFAPEPSYLSGEDIGEFRNMVDALHGAGIEVILDVVYNHSAEGSRLGPTFSFRGIDNLSYYRL
HPNDKRFYINDTGCGNTLNLNHPRMLQLVLDSLRYWVEVMGVDGFRFDLAACLGREAYGFDPGSGFFDALLQDPVLCRVK
LIAEPWDIGPGGYQLGNFPVAFSEWNDRYRDTMRRFWRGDHAMLPEFARRFHGSGDFFEHSGRPPAASINFLTSHDGFTL
KDLVSYCERHNWANGEENRDGHHENFSHHYGVEGDTDDATILALRARQQRNLLTTLFLSQGVPMLLSGDETGRTQGGNNN
AYCQDNPLNWFDWSSDGMDSSLLSFTSQLIALRKRFPLLCAKRFIHEQLVANAFDWSSESSSTQAASPVSTPSGARLDWF
SRQGEPMSKSLWSESMGRSLCVVLSGNLTATQPQTQGDSLQALLLMVNADEHPLAFTLPIFDGLGPWQCLLHTQTDPQFL
TPTEPLDAQSSPTRYLLQDRSLMLFHADFIRN

Sequences:

>Translated_752_residues
MTNPVPVNADGSTAYNLSAGQPFPLGATVDDGGVNFALFSAHATGVELCLFNARGEVEIQRIALTEQTQQIWHLYVHGLS
AGQLYGYRVYGPYEPQLGHRFNPHKLLLDPYARQLVGRYHHHIANFGYEIDNPNEDLSFSTLDNADFVPKCKVVDTRPLL
DAAKAHTIRPLASKREPLPLEQCIIYEMHLKGFTALHPEIDAPLRGTFAGLASNAAIDYLVNLGVNCVELLPVQAFFSEP
FLLEKQLSNYWGYNSIGFFAPEPSYLSGEDIGEFRNMVDALHGAGIEVILDVVYNHSAEGSRLGPTFSFRGIDNLSYYRL
HPNDKRFYINDTGCGNTLNLNHPRMLQLVLDSLRYWVEVMGVDGFRFDLAACLGREAYGFDPGSGFFDALLQDPVLCRVK
LIAEPWDIGPGGYQLGNFPVAFSEWNDRYRDTMRRFWRGDHAMLPEFARRFHGSGDFFEHSGRPPAASINFLTSHDGFTL
KDLVSYCERHNWANGEENRDGHHENFSHHYGVEGDTDDATILALRARQQRNLLTTLFLSQGVPMLLSGDETGRTQGGNNN
AYCQDNPLNWFDWSSDGMDSSLLSFTSQLIALRKRFPLLCAKRFIHEQLVANAFDWSSESSSTQAASPVSTPSGARLDWF
SRQGEPMSKSLWSESMGRSLCVVLSGNLTATQPQTQGDSLQALLLMVNADEHPLAFTLPIFDGLGPWQCLLHTQTDPQFL
TPTEPLDAQSSPTRYLLQDRSLMLFHADFIRN
>Mature_751_residues
TNPVPVNADGSTAYNLSAGQPFPLGATVDDGGVNFALFSAHATGVELCLFNARGEVEIQRIALTEQTQQIWHLYVHGLSA
GQLYGYRVYGPYEPQLGHRFNPHKLLLDPYARQLVGRYHHHIANFGYEIDNPNEDLSFSTLDNADFVPKCKVVDTRPLLD
AAKAHTIRPLASKREPLPLEQCIIYEMHLKGFTALHPEIDAPLRGTFAGLASNAAIDYLVNLGVNCVELLPVQAFFSEPF
LLEKQLSNYWGYNSIGFFAPEPSYLSGEDIGEFRNMVDALHGAGIEVILDVVYNHSAEGSRLGPTFSFRGIDNLSYYRLH
PNDKRFYINDTGCGNTLNLNHPRMLQLVLDSLRYWVEVMGVDGFRFDLAACLGREAYGFDPGSGFFDALLQDPVLCRVKL
IAEPWDIGPGGYQLGNFPVAFSEWNDRYRDTMRRFWRGDHAMLPEFARRFHGSGDFFEHSGRPPAASINFLTSHDGFTLK
DLVSYCERHNWANGEENRDGHHENFSHHYGVEGDTDDATILALRARQQRNLLTTLFLSQGVPMLLSGDETGRTQGGNNNA
YCQDNPLNWFDWSSDGMDSSLLSFTSQLIALRKRFPLLCAKRFIHEQLVANAFDWSSESSSTQAASPVSTPSGARLDWFS
RQGEPMSKSLWSESMGRSLCVVLSGNLTATQPQTQGDSLQALLLMVNADEHPLAFTLPIFDGLGPWQCLLHTQTDPQFLT
PTEPLDAQSSPTRYLLQDRSLMLFHADFIRN

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=658, Percent_Identity=47.2644376899696, Blast_Score=536, Evalue=1e-153,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 84166; Mature: 84035

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNPVPVNADGSTAYNLSAGQPFPLGATVDDGGVNFALFSAHATGVELCLFNARGEVEIQ
CCCCEECCCCCCEEEECCCCCCCCCCCEECCCCCEEEEEECCCCCEEEEEECCCCCEEEE
RIALTEQTQQIWHLYVHGLSAGQLYGYRVYGPYEPQLGHRFNPHKLLLDPYARQLVGRYH
EEEHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCEEEECHHHHHHHHHHH
HHIANFGYEIDNPNEDLSFSTLDNADFVPKCKVVDTRPLLDAAKAHTIRPLASKREPLPL
HHHHHCCCCCCCCCCCCEEEECCCCCCCCCEEEECCCCHHHHHHHHHCCHHHHCCCCCCH
EQCIIYEMHLKGFTALHPEIDAPLRGTFAGLASNAAIDYLVNLGVNCVELLPVQAFFSEP
HHHEEEEEHHCCEEEECCCCCCCCHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHCCC
FLLEKQLSNYWGYNSIGFFAPEPSYLSGEDIGEFRNMVDALHGAGIEVILDVVYNHSAEG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCC
SRLGPTFSFRGIDNLSYYRLHPNDKRFYINDTGCGNTLNLNHPRMLQLVLDSLRYWVEVM
CCCCCEEEECCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
GVDGFRFDLAACLGREAYGFDPGSGFFDALLQDPVLCRVKLIAEPWDIGPGGYQLGNFPV
CCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCE
AFSEWNDRYRDTMRRFWRGDHAMLPEFARRFHGSGDFFEHSGRPPAASINFLTSHDGFTL
EHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHCCCCCCCCEEEEEECCCCCCH
KDLVSYCERHNWANGEENRDGHHENFSHHYGVEGDTDDATILALRARQQRNLLTTLFLSQ
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHC
GVPMLLSGDETGRTQGGNNNAYCQDNPLNWFDWSSDGMDSSLLSFTSQLIALRKRFPLLC
CCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHH
AKRFIHEQLVANAFDWSSESSSTQAASPVSTPSGARLDWFSRQGEPMSKSLWSESMGRSL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEHHCCCCCCHHHHHHHHHCCCEE
CVVLSGNLTATQPQTQGDSLQALLLMVNADEHPLAFTLPIFDGLGPWQCLLHTQTDPQFL
EEEEECCEEECCCCCCCCCEEEEEEEEECCCCCEEEEECHHCCCCHHHEEEECCCCCCEE
TPTEPLDAQSSPTRYLLQDRSLMLFHADFIRN
CCCCCCCCCCCCCEEHHCCCCEEEEEHHHHCC
>Mature Secondary Structure 
TNPVPVNADGSTAYNLSAGQPFPLGATVDDGGVNFALFSAHATGVELCLFNARGEVEIQ
CCCEECCCCCCEEEECCCCCCCCCCCEECCCCCEEEEEECCCCCEEEEEECCCCCEEEE
RIALTEQTQQIWHLYVHGLSAGQLYGYRVYGPYEPQLGHRFNPHKLLLDPYARQLVGRYH
EEEHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCEEEECHHHHHHHHHHH
HHIANFGYEIDNPNEDLSFSTLDNADFVPKCKVVDTRPLLDAAKAHTIRPLASKREPLPL
HHHHHCCCCCCCCCCCCEEEECCCCCCCCCEEEECCCCHHHHHHHHHCCHHHHCCCCCCH
EQCIIYEMHLKGFTALHPEIDAPLRGTFAGLASNAAIDYLVNLGVNCVELLPVQAFFSEP
HHHEEEEEHHCCEEEECCCCCCCCHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHCCC
FLLEKQLSNYWGYNSIGFFAPEPSYLSGEDIGEFRNMVDALHGAGIEVILDVVYNHSAEG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCC
SRLGPTFSFRGIDNLSYYRLHPNDKRFYINDTGCGNTLNLNHPRMLQLVLDSLRYWVEVM
CCCCCEEEECCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
GVDGFRFDLAACLGREAYGFDPGSGFFDALLQDPVLCRVKLIAEPWDIGPGGYQLGNFPV
CCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCE
AFSEWNDRYRDTMRRFWRGDHAMLPEFARRFHGSGDFFEHSGRPPAASINFLTSHDGFTL
EHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHCCCCCCCCEEEEEECCCCCCH
KDLVSYCERHNWANGEENRDGHHENFSHHYGVEGDTDDATILALRARQQRNLLTTLFLSQ
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHC
GVPMLLSGDETGRTQGGNNNAYCQDNPLNWFDWSSDGMDSSLLSFTSQLIALRKRFPLLC
CCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHH
AKRFIHEQLVANAFDWSSESSSTQAASPVSTPSGARLDWFSRQGEPMSKSLWSESMGRSL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEHHCCCCCCHHHHHHHHHCCCEE
CVVLSGNLTATQPQTQGDSLQALLLMVNADEHPLAFTLPIFDGLGPWQCLLHTQTDPQFL
EEEEECCEEECCCCCCCCCEEEEEEEEECCCCCEEEEECHHCCCCHHHEEEECCCCCCEE
TPTEPLDAQSSPTRYLLQDRSLMLFHADFIRN
CCCCCCCCCCCCCEEHHCCCCEEEEEHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]