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The map label for this gene is glgB [H]

Identifier: 117921374

GI number: 117921374

Start: 3521680

End: 3523917

Strand: Reverse

Name: glgB [H]

Synonym: Shewana3_2934

Alternate gene names: 117921374

Gene position: 3523917-3521680 (Counterclockwise)

Preceding gene: 117921375

Following gene: 117921373

Centisome position: 70.87

GC content: 52.37

Gene sequence:

>2238_bases
ATGATGACTCAAGCCCAGACTTACTTCTATGACGGTAGCGATGTTGCGCTGTTAAATGGTCAATATACGGATGTGTTTTC
CCTGCTCGGTATGCACAGCATCAACGAGGGTAAGGCCTTGGTGGTGCGCTGTTTTTTACGCAACGCCCAAAAAGTTGATG
TGATAAGTCTTAAGGATGGCCGTAAGGTAGCGAGCCTTGAGCGCGTTAATGAGGCGGGGCTATTTGCAGGCACGCTTGGC
AGACGGGTTAAGCCTTTTTTGTATGCGCTACGGGTGGTTTATCCCCTCTGCGAGCTGGATATTATCGATCCTTATCAGTT
TGGTTCTCTCCTCGATAGTCAGGACTTATACCTCTTTGGCGAAGGAAGTTCTGAGCAGGCCTATCGCTTTTTAGGTGCGA
ACTGGCGCCACGTCGATAACGTCGAAGGCGTGCATTTTTGCGTATGGGCGCCCAATGCTAAGCGGGTTTCTGTGGTGGGG
GATTTTAACCACTGGGACGATACTCGCCATGTGATGCGCCAACATATGGCCAACGGCTTATGGGAGATTTTTCTGCCAGA
TGTTGCCCAAGGCGCGCACTATAAGTTTGATTTGGTTTATCAAAATGGCGAGCGCCACGCCAAATCCGACCCTATGGCGA
CCCAAATGGAATGTGCACCGCACAATGCCTCCATAGTGCCGAAAAAACACCAACACCCGTGGGCGGATACACAATGGATG
CACAAACGGGCGACCACGGCTTGGCATCGCGCCGCTATGTCTATCTACGAAGTGCAGCTAGGCTCTTGGCGCCGAAAAGG
TGAGTTCGGTGAGCAATATTTTGATTATCAAGACTTAATCGAGCAATTGATCCCCTATGTGAAGGAGCAAGGTTTTACCC
ATATAGAGTTGATGCCAGTCAGCGAGTATCCCTTCGACGGCTCTTGGGGGTATCAACCCGTTGGCCTGTATGCGCCGACC
CATCGTTTTGGCGATGCCAATGGCTTAAAGGCCTTTATCGATGCCTGCCATCAGGCCGAGATTGGTGTGTTACTCGATTG
GGTCGCGGCGCATTTCCCTAAGGATCCCCATGGCCTTGTGCGTTTCGATGGCACTTGCCTCTACGAACATGAAGACCCGC
GTAAAGGTACGCACCCCGATTGGGACACGCTGATCTATAACTACGACCGTGGTGAGGTACGCAGCTTTCTGCTCAGCAAT
GCCTGCTATTGGCTGCGGGAATTTCACCTCGATGGGCTCAGGCTTGATGCGGTGTCATCCATGTTGTACCTCGACTACAG
CCGTGAACCCGGGCAATGGCTGCCCAATGCCTATGGCGGACGGGAGAATTTAGAGGCGATTCATTTTCTGCAAATGTTGA
ATCAACGTTTGTATCAAGCCTTCCCTGGCATTTGCATGATAGCCGAAGAGTCGACCGCCTTTGCGGGGGTGACTAAGCCG
ACGGATTGCGGCGGGCTGGGGTTTGGTTTTAAGTGGAATATGGGCTGGATGAACGACAGCCTAAGCTACTTGGGCCGCGA
TCCCATCTATCGCCAGTATCACCATAACCAGCTGACCTTTAGTTTGATGTATGCCTATTCGGAGCAATTTATGCTGTCGA
TAAGCCACGATGAAGTGGTGCACGGCAAGGGCTCTTTGCTGCATAAAATCCCTGGCGATGACTGGCAAAAATTCGCCACC
CTCAGGGCCTATTACGGCTTTATGTGGGGGCATCCGGGCAAAAAATTACTGTTTATGGGCGGCGAGTTCGCCCAGCGCGA
CGAATGGAACCATAACCATAGCCTCGATTGGCATTTGCTCGCTTTTGAACCCCACCAAGGCGTACAGCGCTGGCTTCGGG
ATCTGAATTGGCTTTATCGACAATTCCCCGCCTTATCCGTGCTCGACTATGAGTCACAGGGGTTTCGCTGGCTTGATTGT
GACAATGGTCGCGACAGTATTTTTAGCTTTGTTCGCTATGGCGAGGGTAGTGATGTGCCCTTGGTGTTTGTGGTGAACAT
GACGCCAACCCTGCACCAAGGATTTCGTATCGGCTTGCCCCAAGGCGGGGATTTTTGTGAGTACCTCAATAGCGACAGCC
ATCTCTATGGCGGTAGCAATCAGGGGAATGCGGGCAAGGTGATTGCCGAAGATTTACCTTGGCAGGGCATGGCATCGAGC
GCGTTGATCACTGTGCCGCCCCTGGGTTGCTTAATCCTCGGGCCTGCGACGGATGCGCCAAGAGATACCAGCCTATGA

Upstream 100 bases:

>100_bases
GGCCTGCGCTGGCTGAGCGTTTGCAACGCATTGCTGCGGCAAGACATTTGCCAGAAACGGCTGCGCATACAGATACCCTT
TGACTAAAAATGGAAGCCAA

Downstream 100 bases:

>100_bases
CGAATCCTGTGCCTGTAAATGCTGATGGCTCAACCGCGTATAACCTAAGTGCGGGGCAGCCCTTTCCCTTGGGGGCGACG
GTCGATGATGGCGGGGTGAA

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE [H]

Number of amino acids: Translated: 745; Mature: 745

Protein sequence:

>745_residues
MMTQAQTYFYDGSDVALLNGQYTDVFSLLGMHSINEGKALVVRCFLRNAQKVDVISLKDGRKVASLERVNEAGLFAGTLG
RRVKPFLYALRVVYPLCELDIIDPYQFGSLLDSQDLYLFGEGSSEQAYRFLGANWRHVDNVEGVHFCVWAPNAKRVSVVG
DFNHWDDTRHVMRQHMANGLWEIFLPDVAQGAHYKFDLVYQNGERHAKSDPMATQMECAPHNASIVPKKHQHPWADTQWM
HKRATTAWHRAAMSIYEVQLGSWRRKGEFGEQYFDYQDLIEQLIPYVKEQGFTHIELMPVSEYPFDGSWGYQPVGLYAPT
HRFGDANGLKAFIDACHQAEIGVLLDWVAAHFPKDPHGLVRFDGTCLYEHEDPRKGTHPDWDTLIYNYDRGEVRSFLLSN
ACYWLREFHLDGLRLDAVSSMLYLDYSREPGQWLPNAYGGRENLEAIHFLQMLNQRLYQAFPGICMIAEESTAFAGVTKP
TDCGGLGFGFKWNMGWMNDSLSYLGRDPIYRQYHHNQLTFSLMYAYSEQFMLSISHDEVVHGKGSLLHKIPGDDWQKFAT
LRAYYGFMWGHPGKKLLFMGGEFAQRDEWNHNHSLDWHLLAFEPHQGVQRWLRDLNWLYRQFPALSVLDYESQGFRWLDC
DNGRDSIFSFVRYGEGSDVPLVFVVNMTPTLHQGFRIGLPQGGDFCEYLNSDSHLYGGSNQGNAGKVIAEDLPWQGMASS
ALITVPPLGCLILGPATDAPRDTSL

Sequences:

>Translated_745_residues
MMTQAQTYFYDGSDVALLNGQYTDVFSLLGMHSINEGKALVVRCFLRNAQKVDVISLKDGRKVASLERVNEAGLFAGTLG
RRVKPFLYALRVVYPLCELDIIDPYQFGSLLDSQDLYLFGEGSSEQAYRFLGANWRHVDNVEGVHFCVWAPNAKRVSVVG
DFNHWDDTRHVMRQHMANGLWEIFLPDVAQGAHYKFDLVYQNGERHAKSDPMATQMECAPHNASIVPKKHQHPWADTQWM
HKRATTAWHRAAMSIYEVQLGSWRRKGEFGEQYFDYQDLIEQLIPYVKEQGFTHIELMPVSEYPFDGSWGYQPVGLYAPT
HRFGDANGLKAFIDACHQAEIGVLLDWVAAHFPKDPHGLVRFDGTCLYEHEDPRKGTHPDWDTLIYNYDRGEVRSFLLSN
ACYWLREFHLDGLRLDAVSSMLYLDYSREPGQWLPNAYGGRENLEAIHFLQMLNQRLYQAFPGICMIAEESTAFAGVTKP
TDCGGLGFGFKWNMGWMNDSLSYLGRDPIYRQYHHNQLTFSLMYAYSEQFMLSISHDEVVHGKGSLLHKIPGDDWQKFAT
LRAYYGFMWGHPGKKLLFMGGEFAQRDEWNHNHSLDWHLLAFEPHQGVQRWLRDLNWLYRQFPALSVLDYESQGFRWLDC
DNGRDSIFSFVRYGEGSDVPLVFVVNMTPTLHQGFRIGLPQGGDFCEYLNSDSHLYGGSNQGNAGKVIAEDLPWQGMASS
ALITVPPLGCLILGPATDAPRDTSL
>Mature_745_residues
MMTQAQTYFYDGSDVALLNGQYTDVFSLLGMHSINEGKALVVRCFLRNAQKVDVISLKDGRKVASLERVNEAGLFAGTLG
RRVKPFLYALRVVYPLCELDIIDPYQFGSLLDSQDLYLFGEGSSEQAYRFLGANWRHVDNVEGVHFCVWAPNAKRVSVVG
DFNHWDDTRHVMRQHMANGLWEIFLPDVAQGAHYKFDLVYQNGERHAKSDPMATQMECAPHNASIVPKKHQHPWADTQWM
HKRATTAWHRAAMSIYEVQLGSWRRKGEFGEQYFDYQDLIEQLIPYVKEQGFTHIELMPVSEYPFDGSWGYQPVGLYAPT
HRFGDANGLKAFIDACHQAEIGVLLDWVAAHFPKDPHGLVRFDGTCLYEHEDPRKGTHPDWDTLIYNYDRGEVRSFLLSN
ACYWLREFHLDGLRLDAVSSMLYLDYSREPGQWLPNAYGGRENLEAIHFLQMLNQRLYQAFPGICMIAEESTAFAGVTKP
TDCGGLGFGFKWNMGWMNDSLSYLGRDPIYRQYHHNQLTFSLMYAYSEQFMLSISHDEVVHGKGSLLHKIPGDDWQKFAT
LRAYYGFMWGHPGKKLLFMGGEFAQRDEWNHNHSLDWHLLAFEPHQGVQRWLRDLNWLYRQFPALSVLDYESQGFRWLDC
DNGRDSIFSFVRYGEGSDVPLVFVVNMTPTLHQGFRIGLPQGGDFCEYLNSDSHLYGGSNQGNAGKVIAEDLPWQGMASS
ALITVPPLGCLILGPATDAPRDTSL

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position [H]

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI189458812, Length=715, Percent_Identity=26.7132867132867, Blast_Score=171, Evalue=2e-42,
Organism=Escherichia coli, GI1789839, Length=724, Percent_Identity=50.1381215469613, Blast_Score=711, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17554896, Length=703, Percent_Identity=25.74679943101, Blast_Score=188, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI32564391, Length=626, Percent_Identity=24.9201277955272, Blast_Score=159, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6320826, Length=655, Percent_Identity=25.0381679389313, Blast_Score=172, Evalue=2e-43,
Organism=Drosophila melanogaster, GI28573410, Length=642, Percent_Identity=26.4797507788162, Blast_Score=177, Evalue=3e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48 [H]

EC number: =2.4.1.18 [H]

Molecular weight: Translated: 85185; Mature: 85185

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00435 PEROXIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTQAQTYFYDGSDVALLNGQYTDVFSLLGMHSINEGKALVVRCFLRNAQKVDVISLKDG
CCCCCCEEEECCCCEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEEECCC
RKVASLERVNEAGLFAGTLGRRVKPFLYALRVVYPLCELDIIDPYQFGSLLDSQDLYLFG
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEE
EGSSEQAYRFLGANWRHVDNVEGVHFCVWAPNAKRVSVVGDFNHWDDTRHVMRQHMANGL
CCCCHHHHHHHCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHCCE
WEIFLPDVAQGAHYKFDLVYQNGERHAKSDPMATQMECAPHNASIVPKKHQHPWADTQWM
EEEECHHHCCCCCEEEEEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHH
HKRATTAWHRAAMSIYEVQLGSWRRKGEFGEQYFDYQDLIEQLIPYVKEQGFTHIELMPV
HHHHHHHHHHHHHHHEEEHHCCCCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEC
SEYPFDGSWGYQPVGLYAPTHRFGDANGLKAFIDACHQAEIGVLLDWVAAHFPKDPHGLV
CCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEE
RFDGTCLYEHEDPRKGTHPDWDTLIYNYDRGEVRSFLLSNACYWLREFHLDGLRLDAVSS
EECCEEEECCCCCCCCCCCCHHHEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHH
MLYLDYSREPGQWLPNAYGGRENLEAIHFLQMLNQRLYQAFPGICMIAEESTAFAGVTKP
EEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEECCCCC
TDCGGLGFGFKWNMGWMNDSLSYLGRDPIYRQYHHNQLTFSLMYAYSEQFMLSISHDEVV
CCCCCCCCCEEECCCCCCCCHHHHCCCCHHHHHCCCCEEEEEEEECCCEEEEEECCCCEE
HGKGSLLHKIPGDDWQKFATLRAYYGFMWGHPGKKLLFMGGEFAQRDEWNHNHSLDWHLL
CCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHCCCCCCCCCCCEEEEE
AFEPHQGVQRWLRDLNWLYRQFPALSVLDYESQGFRWLDCDNGRDSIFSFVRYGEGSDVP
EECCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEECCCCHHHHHHHHHCCCCCCCC
LVFVVNMTPTLHQGFRIGLPQGGDFCEYLNSDSHLYGGSNQGNAGKVIAEDLPWQGMASS
EEEEEECCCHHHCCEEECCCCCCCHHHHCCCCCEEECCCCCCCCCCEEECCCCCCCCCCC
ALITVPPLGCLILGPATDAPRDTSL
EEEEECCCCEEEECCCCCCCCCCCC
>Mature Secondary Structure
MMTQAQTYFYDGSDVALLNGQYTDVFSLLGMHSINEGKALVVRCFLRNAQKVDVISLKDG
CCCCCCEEEECCCCEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEEECCC
RKVASLERVNEAGLFAGTLGRRVKPFLYALRVVYPLCELDIIDPYQFGSLLDSQDLYLFG
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEE
EGSSEQAYRFLGANWRHVDNVEGVHFCVWAPNAKRVSVVGDFNHWDDTRHVMRQHMANGL
CCCCHHHHHHHCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHCCE
WEIFLPDVAQGAHYKFDLVYQNGERHAKSDPMATQMECAPHNASIVPKKHQHPWADTQWM
EEEECHHHCCCCCEEEEEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHH
HKRATTAWHRAAMSIYEVQLGSWRRKGEFGEQYFDYQDLIEQLIPYVKEQGFTHIELMPV
HHHHHHHHHHHHHHHEEEHHCCCCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEC
SEYPFDGSWGYQPVGLYAPTHRFGDANGLKAFIDACHQAEIGVLLDWVAAHFPKDPHGLV
CCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEE
RFDGTCLYEHEDPRKGTHPDWDTLIYNYDRGEVRSFLLSNACYWLREFHLDGLRLDAVSS
EECCEEEECCCCCCCCCCCCHHHEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHH
MLYLDYSREPGQWLPNAYGGRENLEAIHFLQMLNQRLYQAFPGICMIAEESTAFAGVTKP
EEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEECCCCC
TDCGGLGFGFKWNMGWMNDSLSYLGRDPIYRQYHHNQLTFSLMYAYSEQFMLSISHDEVV
CCCCCCCCCEEECCCCCCCCHHHHCCCCHHHHHCCCCEEEEEEEECCCEEEEEECCCCEE
HGKGSLLHKIPGDDWQKFATLRAYYGFMWGHPGKKLLFMGGEFAQRDEWNHNHSLDWHLL
CCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHCCCCCCCCCCCEEEEE
AFEPHQGVQRWLRDLNWLYRQFPALSVLDYESQGFRWLDCDNGRDSIFSFVRYGEGSDVP
EECCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEECCCCHHHHHHHHHCCCCCCCC
LVFVVNMTPTLHQGFRIGLPQGGDFCEYLNSDSHLYGGSNQGNAGKVIAEDLPWQGMASS
EEEEEECCCHHHCCEEECCCCCCCHHHHCCCCCEEECCCCCCCCCCEEECCCCCCCCCCC
ALITVPPLGCLILGPATDAPRDTSL
EEEEECCCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]