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The map label for this gene is gcvA [H]

Identifier: 117921335

GI number: 117921335

Start: 3463675

End: 3464586

Strand: Reverse

Name: gcvA [H]

Synonym: Shewana3_2894

Alternate gene names: 117921335

Gene position: 3464586-3463675 (Counterclockwise)

Preceding gene: 117921337

Following gene: 117921334

Centisome position: 69.68

GC content: 50.33

Gene sequence:

>912_bases
ATGTCAAGACGCTTACCACCACTGAATGCGGTTAAAGCATTTGAGGCCGCCGCACGGCATTTGAGCTTTACTCGAGCGGC
TGAAGAATTGTTTGTGACTCAGGCTGCGGTGAGTCACCAAATTAAGGCGCTGGAAGATTTTCTCGGATTAAAATTATTTC
GCCGAAAGAATCGTTCGCTGCTGCTGACCGAAGAAGGTCAGAGCTATTTTCTCGATATTAAAGACATCTTCACCCAACTG
GCGGATGCGACCGACAGGCTGCTCGCACGTAGTGCCATTGGTTCGTTGACCGTGAGTATGTCGCCAAGCTTTGCGATTCA
GTGGCTGGTGCCGCGCCTGTCGAAGTTTAGTGAAAAAAATCCCGACATTGACGTACGAATTAAAGCGGTAGACACAGAGG
CTAGCTCCTTAACCGACGATGTCGACGTTGCCATTTATTATGGTTTAGGCAATTGGCCTGGGCTTCGCGCCGATAAACTC
CGTAATGAAGTGCTGATCCCCGTATGTTCGCCTATGCTGTTAAACGGCCCAAAGCCACTATCAAAGCCAGAAGATTTAAA
ATACCACACGCTGCTGCACGATATGAGCCGTCACGATTGGCAGGCTTGGTTTAGACAATGTGGCATTCACGATATCAACG
TCAACCAAGGGCCGATTTTTAGCCATTCCTCCCTCGTTTTGCAGGCGGCTGCCCACGGCCAAGGTGTGGCGTTAGGTTAT
AGCGTACTCGCTCGCCCCGATATTAAGGCGGGTCGACTGGTGTGCCCATTCCAAGAAGTGTTAGTGAGCAAAGATGCCTA
TTACCTCGTCTGCCAACAGAATCATGCCGAGCTGGGTAAAGTGGTCGCGTTCCGTGAGTGGATGTTAGATATGTTTGCCG
AGGAGTCACGCAGTGAGTTGTTACCCGGATAA

Upstream 100 bases:

>100_bases
CGTTGGCGCAAAATTTAATCAAAAACGCTGGCCTTTACAAACGATTAAATGTAACAATACGGATTAGAAAAACTAATTAA
AAATTGAGATAGAGATCACT

Downstream 100 bases:

>100_bases
CCCCTTGGTCAATGAGGATGTGGTGCCAAGTGCCTATGTGTTAGAAGGCGAGCCCTCAGAGACCTTGATTTTATTTGCCC
ACGGCGCGGGAGCGAATCGA

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MSRRLPPLNAVKAFEAAARHLSFTRAAEELFVTQAAVSHQIKALEDFLGLKLFRRKNRSLLLTEEGQSYFLDIKDIFTQL
ADATDRLLARSAIGSLTVSMSPSFAIQWLVPRLSKFSEKNPDIDVRIKAVDTEASSLTDDVDVAIYYGLGNWPGLRADKL
RNEVLIPVCSPMLLNGPKPLSKPEDLKYHTLLHDMSRHDWQAWFRQCGIHDINVNQGPIFSHSSLVLQAAAHGQGVALGY
SVLARPDIKAGRLVCPFQEVLVSKDAYYLVCQQNHAELGKVVAFREWMLDMFAEESRSELLPG

Sequences:

>Translated_303_residues
MSRRLPPLNAVKAFEAAARHLSFTRAAEELFVTQAAVSHQIKALEDFLGLKLFRRKNRSLLLTEEGQSYFLDIKDIFTQL
ADATDRLLARSAIGSLTVSMSPSFAIQWLVPRLSKFSEKNPDIDVRIKAVDTEASSLTDDVDVAIYYGLGNWPGLRADKL
RNEVLIPVCSPMLLNGPKPLSKPEDLKYHTLLHDMSRHDWQAWFRQCGIHDINVNQGPIFSHSSLVLQAAAHGQGVALGY
SVLARPDIKAGRLVCPFQEVLVSKDAYYLVCQQNHAELGKVVAFREWMLDMFAEESRSELLPG
>Mature_302_residues
SRRLPPLNAVKAFEAAARHLSFTRAAEELFVTQAAVSHQIKALEDFLGLKLFRRKNRSLLLTEEGQSYFLDIKDIFTQLA
DATDRLLARSAIGSLTVSMSPSFAIQWLVPRLSKFSEKNPDIDVRIKAVDTEASSLTDDVDVAIYYGLGNWPGLRADKLR
NEVLIPVCSPMLLNGPKPLSKPEDLKYHTLLHDMSRHDWQAWFRQCGIHDINVNQGPIFSHSSLVLQAAAHGQGVALGYS
VLARPDIKAGRLVCPFQEVLVSKDAYYLVCQQNHAELGKVVAFREWMLDMFAEESRSELLPG

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=295, Percent_Identity=71.5254237288136, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI1786448, Length=287, Percent_Identity=36.5853658536585, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1788706, Length=288, Percent_Identity=30.9027777777778, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI145693193, Length=301, Percent_Identity=26.2458471760797, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1786401, Length=242, Percent_Identity=29.7520661157025, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI157672245, Length=209, Percent_Identity=28.7081339712919, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI87081978, Length=298, Percent_Identity=28.8590604026846, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787879, Length=128, Percent_Identity=33.59375, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789639, Length=194, Percent_Identity=26.2886597938144, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1787128, Length=135, Percent_Identity=29.6296296296296, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1788887, Length=128, Percent_Identity=32.8125, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790208, Length=244, Percent_Identity=27.0491803278689, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33945; Mature: 33814

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRLPPLNAVKAFEAAARHLSFTRAAEELFVTQAAVSHQIKALEDFLGLKLFRRKNRSL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
LLTEEGQSYFLDIKDIFTQLADATDRLLARSAIGSLTVSMSPSFAIQWLVPRLSKFSEKN
EEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHHHHHCCC
PDIDVRIKAVDTEASSLTDDVDVAIYYGLGNWPGLRADKLRNEVLIPVCSPMLLNGPKPL
CCEEEEEEEECCCHHHCCCCCEEEEEEECCCCCCCCHHHHCCCEEEEECCHHHCCCCCCC
SKPEDLKYHTLLHDMSRHDWQAWFRQCGIHDINVNQGPIFSHSSLVLQAAAHGQGVALGY
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHEEHHCCCCCEEEHH
SVLARPDIKAGRLVCPFQEVLVSKDAYYLVCQQNHAELGKVVAFREWMLDMFAEESRSEL
HHHCCCCCCCCCEECCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHC
LPG
CCC
>Mature Secondary Structure 
SRRLPPLNAVKAFEAAARHLSFTRAAEELFVTQAAVSHQIKALEDFLGLKLFRRKNRSL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
LLTEEGQSYFLDIKDIFTQLADATDRLLARSAIGSLTVSMSPSFAIQWLVPRLSKFSEKN
EEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHHHHHCCC
PDIDVRIKAVDTEASSLTDDVDVAIYYGLGNWPGLRADKLRNEVLIPVCSPMLLNGPKPL
CCEEEEEEEECCCHHHCCCCCEEEEEEECCCCCCCCHHHHCCCEEEEECCHHHCCCCCCC
SKPEDLKYHTLLHDMSRHDWQAWFRQCGIHDINVNQGPIFSHSSLVLQAAAHGQGVALGY
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHEEHHCCCCCEEEHH
SVLARPDIKAGRLVCPFQEVLVSKDAYYLVCQQNHAELGKVVAFREWMLDMFAEESRSEL
HHHCCCCCCCCCEECCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHC
LPG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]