Click here to switch to the map view.

The map label for this gene is dosC [H]

Identifier: 117921325

GI number: 117921325

Start: 3451132

End: 3452688

Strand: Direct

Name: dosC [H]

Synonym: Shewana3_2884

Alternate gene names: 117921325

Gene position: 3451132-3452688 (Clockwise)

Preceding gene: 117921324

Following gene: 117921326

Centisome position: 69.41

GC content: 45.47

Gene sequence:

>1557_bases
ATGAAGGATTCCAACACGACAGTTTCACAACTGGCATTACTCCAACAGAAACTTCACTCTGCCAAGTTGGCACTCGATGA
AGTCAATGAAGATAGAAATGCTAAGTTACAAACCTTACTCCAATTTATTGGCCATTTAAGTCTTGCCTGCAAAGGTCAGA
ATTTAGAACTGGATAATAAACTCGCCAAACTTCGCCATAACTTAAATACCTTTGAGCGAGTCGATGAAGCGTTGCCGGAA
TTAGTCGATGTTGAACGCCTATTAAAGGGTCAATACCACCATGTGATGGTACAACTTGAAGAGAGCCGTACTGGGCTTGC
GCAAGTCATTCGCCAAATTCAACGGGTAAACTCAGCGCCAGATAAACTCAAAAAAGAAGTTAATTACTTTAAGCAGGATC
TCGCCAAACCTTTCCATACGGTGTGGGAATATATTCCTAAAGTAAAACAAATCATTGGTTTCTATGACACCATTTTGCAG
CAGCAATTTGAAGAAGCCGAAAAACTCGATGTGTTACCGAAACACAGGCAGCTCGCCCACGAATTGGCGCAGATGATCTC
GGAAATCGAGTTCCGTAAAGATCAACGCGACCAAGTACTGGTCATTAAAGAGCTACTCGCTTCAGAAGTCGAAATCGATA
CCCTGCTCGAAGCCTATCAAACCATTTTATCCCTGCTGCTCGATAACATCGCCCGCGAAAAATCGGCCTCGCAAGAATTT
TTATACGCACTAAACGATGCCTTAGCCGCGGTGCGTGAAGTGGTGAGTGAATCCTACAATCACAATCAGCGCAGCTTCCA
GCTGAAGACTCAGTTGAATCGGGAAATTAATTCGCGCGTGGATAACGTAGGTGAAGCCATTATCGACACCGACGATATTG
TCGATCTTAAAGCACAATTAACCGAACAGCTTAGCTCGATTCGCTCCGCCCTCGCCCGTAAAGAAGCATTAGAACAGCGT
GAGCAGGCCCTGCTGCGCAAATCAATGGAAACCATGCGCAAAGAGCTGAATGAGCTCAGTAGCGAAGCGAATACCTATAA
AGAACGGTTGTTCGAGCAACAAAAACTCAATCTATTGGATAGCTTAACTCAACTGCCTAACCGCGCCGCCCTCGAAGAGC
GCATGGAGCTTGAGTATCGTAATTACCAACGCCACAGAACACCGCTGTGGATTGCAGTAGCAGACATCGACCATTTCAAG
AGCATCAACGACAGCTTTGGTCACAGCACGGGCGATAAAACCCTGCAGGTTATCGCCATGGCATTAAAGAACTCCCTACG
GGATACCGAGTTTGTTGCTCGCTACGGCGGCGAAGAGTTTGTGCTCATCCTGCCTGAAATTGACGGCGGCGATATTGCTC
AACTTTTAAACAGAGTCCGTGAAAAAGTAAAAAATATTCCATTTAAATTTAAAAATCAGCGAATTACAGTTACAGTATCT
ATAGGGGCTGCGCAGGTTATGGGGAACGAGCTCATACATGAAACCTTCGAGCGCGCCGATGCAGCACTCTACAAAGCAAA
ACATGAAAGCAGAGACAGGGTTGTGATTGACGTATAA

Upstream 100 bases:

>100_bases
CACTGCCGTTGTTATAACATTAGTAGCATATGAGTTAACTGCTAATCTAGACAGAGGACGTCATGCTTTTTAGCCCTGCT
GAGTTAGGAAAATACTCCGG

Downstream 100 bases:

>100_bases
CAAAGTCAGCCTATACGTCGCCTCTTATCACTCCTGTCGAGACGAAGGAGTGATAAATGATAGTAAAAGTCAGCCCAAAG
GGCAGCATGGATCAGCTTTC

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MKDSNTTVSQLALLQQKLHSAKLALDEVNEDRNAKLQTLLQFIGHLSLACKGQNLELDNKLAKLRHNLNTFERVDEALPE
LVDVERLLKGQYHHVMVQLEESRTGLAQVIRQIQRVNSAPDKLKKEVNYFKQDLAKPFHTVWEYIPKVKQIIGFYDTILQ
QQFEEAEKLDVLPKHRQLAHELAQMISEIEFRKDQRDQVLVIKELLASEVEIDTLLEAYQTILSLLLDNIAREKSASQEF
LYALNDALAAVREVVSESYNHNQRSFQLKTQLNREINSRVDNVGEAIIDTDDIVDLKAQLTEQLSSIRSALARKEALEQR
EQALLRKSMETMRKELNELSSEANTYKERLFEQQKLNLLDSLTQLPNRAALEERMELEYRNYQRHRTPLWIAVADIDHFK
SINDSFGHSTGDKTLQVIAMALKNSLRDTEFVARYGGEEFVLILPEIDGGDIAQLLNRVREKVKNIPFKFKNQRITVTVS
IGAAQVMGNELIHETFERADAALYKAKHESRDRVVIDV

Sequences:

>Translated_518_residues
MKDSNTTVSQLALLQQKLHSAKLALDEVNEDRNAKLQTLLQFIGHLSLACKGQNLELDNKLAKLRHNLNTFERVDEALPE
LVDVERLLKGQYHHVMVQLEESRTGLAQVIRQIQRVNSAPDKLKKEVNYFKQDLAKPFHTVWEYIPKVKQIIGFYDTILQ
QQFEEAEKLDVLPKHRQLAHELAQMISEIEFRKDQRDQVLVIKELLASEVEIDTLLEAYQTILSLLLDNIAREKSASQEF
LYALNDALAAVREVVSESYNHNQRSFQLKTQLNREINSRVDNVGEAIIDTDDIVDLKAQLTEQLSSIRSALARKEALEQR
EQALLRKSMETMRKELNELSSEANTYKERLFEQQKLNLLDSLTQLPNRAALEERMELEYRNYQRHRTPLWIAVADIDHFK
SINDSFGHSTGDKTLQVIAMALKNSLRDTEFVARYGGEEFVLILPEIDGGDIAQLLNRVREKVKNIPFKFKNQRITVTVS
IGAAQVMGNELIHETFERADAALYKAKHESRDRVVIDV
>Mature_518_residues
MKDSNTTVSQLALLQQKLHSAKLALDEVNEDRNAKLQTLLQFIGHLSLACKGQNLELDNKLAKLRHNLNTFERVDEALPE
LVDVERLLKGQYHHVMVQLEESRTGLAQVIRQIQRVNSAPDKLKKEVNYFKQDLAKPFHTVWEYIPKVKQIIGFYDTILQ
QQFEEAEKLDVLPKHRQLAHELAQMISEIEFRKDQRDQVLVIKELLASEVEIDTLLEAYQTILSLLLDNIAREKSASQEF
LYALNDALAAVREVVSESYNHNQRSFQLKTQLNREINSRVDNVGEAIIDTDDIVDLKAQLTEQLSSIRSALARKEALEQR
EQALLRKSMETMRKELNELSSEANTYKERLFEQQKLNLLDSLTQLPNRAALEERMELEYRNYQRHRTPLWIAVADIDHFK
SINDSFGHSTGDKTLQVIAMALKNSLRDTEFVARYGGEEFVLILPEIDGGDIAQLLNRVREKVKNIPFKFKNQRITVTVS
IGAAQVMGNELIHETFERADAALYKAKHESRDRVVIDV

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=161, Percent_Identity=37.888198757764, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI1787262, Length=160, Percent_Identity=36.25, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787802, Length=160, Percent_Identity=34.375, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787816, Length=158, Percent_Identity=33.5443037974684, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI87082007, Length=158, Percent_Identity=35.4430379746835, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI87081881, Length=121, Percent_Identity=42.9752066115703, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1786584, Length=171, Percent_Identity=32.7485380116959, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1788381, Length=162, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788956, Length=161, Percent_Identity=33.5403726708075, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI87081974, Length=159, Percent_Identity=30.8176100628931, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787541, Length=190, Percent_Identity=25.2631578947368, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787056, Length=172, Percent_Identity=31.3953488372093, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI87081977, Length=106, Percent_Identity=33.9622641509434, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 59701; Mature: 59701

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDSNTTVSQLALLQQKLHSAKLALDEVNEDRNAKLQTLLQFIGHLSLACKGQNLELDNK
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHH
LAKLRHNLNTFERVDEALPELVDVERLLKGQYHHVMVQLEESRTGLAQVIRQIQRVNSAP
HHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEEHHHHHHHHHHHHHHHHHCCCH
DKLKKEVNYFKQDLAKPFHTVWEYIPKVKQIIGFYDTILQQQFEEAEKLDVLPKHRQLAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ELAQMISEIEFRKDQRDQVLVIKELLASEVEIDTLLEAYQTILSLLLDNIAREKSASQEF
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYALNDALAAVREVVSESYNHNQRSFQLKTQLNREINSRVDNVGEAIIDTDDIVDLKAQL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
TEQLSSIRSALARKEALEQREQALLRKSMETMRKELNELSSEANTYKERLFEQQKLNLLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLTQLPNRAALEERMELEYRNYQRHRTPLWIAVADIDHFKSINDSFGHSTGDKTLQVIAM
HHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHCCHHCCCCCCHHHHHHHHH
ALKNSLRDTEFVARYGGEEFVLILPEIDGGDIAQLLNRVREKVKNIPFKFKNQRITVTVS
HHHHHCCHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEECCCEEEEEEE
IGAAQVMGNELIHETFERADAALYKAKHESRDRVVIDV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEC
>Mature Secondary Structure
MKDSNTTVSQLALLQQKLHSAKLALDEVNEDRNAKLQTLLQFIGHLSLACKGQNLELDNK
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHH
LAKLRHNLNTFERVDEALPELVDVERLLKGQYHHVMVQLEESRTGLAQVIRQIQRVNSAP
HHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEEHHHHHHHHHHHHHHHHHCCCH
DKLKKEVNYFKQDLAKPFHTVWEYIPKVKQIIGFYDTILQQQFEEAEKLDVLPKHRQLAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ELAQMISEIEFRKDQRDQVLVIKELLASEVEIDTLLEAYQTILSLLLDNIAREKSASQEF
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYALNDALAAVREVVSESYNHNQRSFQLKTQLNREINSRVDNVGEAIIDTDDIVDLKAQL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
TEQLSSIRSALARKEALEQREQALLRKSMETMRKELNELSSEANTYKERLFEQQKLNLLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLTQLPNRAALEERMELEYRNYQRHRTPLWIAVADIDHFKSINDSFGHSTGDKTLQVIAM
HHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHCCHHCCCCCCHHHHHHHHH
ALKNSLRDTEFVARYGGEEFVLILPEIDGGDIAQLLNRVREKVKNIPFKFKNQRITVTVS
HHHHHCCHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEECCCEEEEEEE
IGAAQVMGNELIHETFERADAALYKAKHESRDRVVIDV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]