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The map label for this gene is tsf [H]

Identifier: 117921255

GI number: 117921255

Start: 3360814

End: 3361665

Strand: Reverse

Name: tsf [H]

Synonym: Shewana3_2814

Alternate gene names: 117921255

Gene position: 3361665-3360814 (Counterclockwise)

Preceding gene: 117921256

Following gene: 117921254

Centisome position: 67.61

GC content: 45.07

Gene sequence:

>852_bases
ATGGCAATTTCTGCTGCCCAAGTTAAAGAACTGCGCGAGCGTACTGGCGCAGGCATGATGGATTGTAAAAAAGCGTTAGA
AGAAACCAACGGCGACATGGAGTTAGCAATTGACAACATGCGTAAGTCTGGTGCTGCTAAAGCTGCTAAGAAAGCAGGTA
ACATCGCTGCTGACGGTACTATCCTGATCAAAAACGGTGAAGGTTTCGCAGTACTGTTAGAAGTAAACTGCCAAACTGAC
TTCGTTGCTAAAGATGCGAACTTCTTAGGTTTTGCTAACGCTGTTTTAGACGTTGCTGCAGCGTCTAAAGTGTCTTTAGA
AGACTTAAAAGCCCAATTCGAAGAAGCCCGTGTTGCTCTGGTTGCTAAAATCGGTGAAAACATCAACGTTCGTCGCGTTG
AGTACATCGACGGCACTCAATTAGCTTCTTACCGTCACGGTGAGCGTATTGGTGTTGTAGTAACTGGTGAAGCGGATGAG
GAAACTCTGAAGCACCTAGCAATGCACGTTGCTGCTTCTAAGCCAGAATACGTTAACCCAGAAGACGTTCCAGCTGACGT
AGTCGCTCGTGAACAAGCTCTGCAAATCGAAATCTCTATGAATGAAGGCAAGCCTGCTGAAATCGCAGAGAAAATGGTTG
TTGGTCGTATGAAGAAGTTCACCGGTGAGATCTCTCTGACTGGTCAAGCTTACATCATGGAACCAAAGAAAACTGTTGGC
GAATTCTTGAAAGAGAAAGGCGCTAAAGTCACTAACTTCATTCGTTTAGAAGTGGGTGAAGGTATTGAGAAGAAAGAAGA
AGATTTCGCAGCTGAAGTTGCTGCTCAAATCGCCGCTTCTAAAAAGGCTTAA

Upstream 100 bases:

>100_bases
TCATCACCCTTATTAACCAAGATTTTGATTGGTTAACAGGGGCCATTCGGCCCCTGTTTTATATCTGCAAATTAGTCGAA
TTTTTAGAGAGGATTTAACA

Downstream 100 bases:

>100_bases
TCGCCAACGCTAAATACCCCCAAGACCGCAGCAATGGCTGCGGTCTTGTTATGACCAGGATATAAAAATTATGAGCACCA
ATCCAAAACCTGCATTTAGA

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts [H]

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MAISAAQVKELRERTGAGMMDCKKALEETNGDMELAIDNMRKSGAAKAAKKAGNIAADGTILIKNGEGFAVLLEVNCQTD
FVAKDANFLGFANAVLDVAAASKVSLEDLKAQFEEARVALVAKIGENINVRRVEYIDGTQLASYRHGERIGVVVTGEADE
ETLKHLAMHVAASKPEYVNPEDVPADVVAREQALQIEISMNEGKPAEIAEKMVVGRMKKFTGEISLTGQAYIMEPKKTVG
EFLKEKGAKVTNFIRLEVGEGIEKKEEDFAAEVAAQIAASKKA

Sequences:

>Translated_283_residues
MAISAAQVKELRERTGAGMMDCKKALEETNGDMELAIDNMRKSGAAKAAKKAGNIAADGTILIKNGEGFAVLLEVNCQTD
FVAKDANFLGFANAVLDVAAASKVSLEDLKAQFEEARVALVAKIGENINVRRVEYIDGTQLASYRHGERIGVVVTGEADE
ETLKHLAMHVAASKPEYVNPEDVPADVVAREQALQIEISMNEGKPAEIAEKMVVGRMKKFTGEISLTGQAYIMEPKKTVG
EFLKEKGAKVTNFIRLEVGEGIEKKEEDFAAEVAAQIAASKKA
>Mature_282_residues
AISAAQVKELRERTGAGMMDCKKALEETNGDMELAIDNMRKSGAAKAAKKAGNIAADGTILIKNGEGFAVLLEVNCQTDF
VAKDANFLGFANAVLDVAAASKVSLEDLKAQFEEARVALVAKIGENINVRRVEYIDGTQLASYRHGERIGVVVTGEADEE
TLKHLAMHVAASKPEYVNPEDVPADVVAREQALQIEISMNEGKPAEIAEKMVVGRMKKFTGEISLTGQAYIMEPKKTVGE
FLKEKGAKVTNFIRLEVGEGIEKKEEDFAAEVAAQIAASKKA

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome [H]

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family [H]

Homologues:

Organism=Homo sapiens, GI171846268, Length=290, Percent_Identity=26.2068965517241, Blast_Score=78, Evalue=8e-15,
Organism=Escherichia coli, GI1786366, Length=275, Percent_Identity=69.8181818181818, Blast_Score=379, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17561440, Length=292, Percent_Identity=26.027397260274, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI19921466, Length=287, Percent_Identity=27.8745644599303, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449 [H]

Pfam domain/function: PF00889 EF_TS; PF00627 UBA [H]

EC number: NA

Molecular weight: Translated: 30458; Mature: 30327

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS01126 EF_TS_1 ; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAISAAQVKELRERTGAGMMDCKKALEETNGDMELAIDNMRKSGAAKAAKKAGNIAADGT
CCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEHHHHHCCHHHHHHHCCCEECCCE
ILIKNGEGFAVLLEVNCQTDFVAKDANFLGFANAVLDVAAASKVSLEDLKAQFEEARVAL
EEEECCCCEEEEEEECCCCCEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VAKIGENINVRRVEYIDGTQLASYRHGERIGVVVTGEADEETLKHLAMHVAASKPEYVNP
HHHHCCCCCEEEEEEECCCHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCC
EDVPADVVAREQALQIEISMNEGKPAEIAEKMVVGRMKKFTGEISLTGQAYIMEPKKTVG
CCCCHHHHHCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEECCCEEEECCHHHHH
EFLKEKGAKVTNFIRLEVGEGIEKKEEDFAAEVAAQIAASKKA
HHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AISAAQVKELRERTGAGMMDCKKALEETNGDMELAIDNMRKSGAAKAAKKAGNIAADGT
CCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEHHHHHCCHHHHHHHCCCEECCCE
ILIKNGEGFAVLLEVNCQTDFVAKDANFLGFANAVLDVAAASKVSLEDLKAQFEEARVAL
EEEECCCCEEEEEEECCCCCEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VAKIGENINVRRVEYIDGTQLASYRHGERIGVVVTGEADEETLKHLAMHVAASKPEYVNP
HHHHCCCCCEEEEEEECCCHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCC
EDVPADVVAREQALQIEISMNEGKPAEIAEKMVVGRMKKFTGEISLTGQAYIMEPKKTVG
CCCCHHHHHCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEECCCEEEECCHHHHH
EFLKEKGAKVTNFIRLEVGEGIEKKEEDFAAEVAAQIAASKKA
HHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA