Click here to switch to the map view.

The map label for this gene is tilS [H]

Identifier: 117921240

GI number: 117921240

Start: 3342683

End: 3344080

Strand: Reverse

Name: tilS [H]

Synonym: Shewana3_2799

Alternate gene names: 117921240

Gene position: 3344080-3342683 (Counterclockwise)

Preceding gene: 117921241

Following gene: 117921238

Centisome position: 67.26

GC content: 52.86

Gene sequence:

>1398_bases
ATGGCGGCGGTGGAACTTTGTGCCCATATAGCGCGATTTTTAGCGAGCTTACCACTGCAAGCGGGGAGCAAGTTAGTCCT
CGCTTACAGTGGCGGTGTCGATTCCGAAGTGCTCGCCTACGGTTTGAGTGAATTCGCCAAACATCACCCCGAGTTTCATT
ATCAGCTGATTTATGTGCACCATGGCTTGAGCCCTAATGCCGACGCTTGGGCCGAGCACTGCCAAACTCGCGCCGCCAAC
TATGGTTTACCAATCACGGTGGAACGAGTACAACTCACCCTCGGGCCGAGAGTGAGTATTGAGGCCGAGGCGCGAAAAAT
GCGTTATCAGGCGATATTGCAGCACCTTAAGCCTCAGGATGTACTGCTGACCGCCCACCATGAAGACGACCAACTCGAGA
CGATTCTACTCGCCCTCAAACGCGGTCAGGGCCCCAAAGGGTTAGCCGCCATGGGGCAAATCCAAATGCTCCCACTCGGC
GAGAAGGGGGCTTGCCTGCAAGTGCGTCCCTTGCTCGATATTAGCCGTGAGCAGATTGAAGCTTTCGCTCAAACCCGACA
GCTAGTGCATATCGAAGATGAAAGTAATCAAGACGATAAATACGATCGTAACTTTTTACGCCTCGAGATTATCCCACGCC
TAAAAGCACGTTGGCCCAGTATCGCCACCACTGCAAGCCGTAGCGCTCAGCTTTGCGCGGAACAACAGGCTGTAGTGGAT
GCCGAGGTGAGTGAGCGTTTACCTAAGCTGTTAACCGAGGCGCACTTTACCCAGCAAACCGTGCTTAAGCTTGATGAGCT
GGCTACACAAACCAGTGAATGGCAGGGATTGCTGCTGCGGGGCTTTATCGAATCACAAGGCTTTGGACTGCCTTCCTATG
TGCAGTTGCAGCAAATCCTGCAGCAGTTAATGGGGGCTAAAGAGGATGCCAAAGTACAGCTCCAGATTGGTGACTGTGTT
CTGCGCCGCTTTGCAGGCATGCTTTATCTGGATACGGTAAACACCGTATCCGCCGCGCCGCGCATCACTACGCGCGAACT
GCATCAAGATATTCTGGCTTTACTCACTCAAGCATCAACAAGAGTTGAGGATAAAATTGCGCCATTTACCTTAGCTACAA
CGGGTCCTCGGCTGTGTTTACCCAAGGCAGGTGAGGTGGTGAGCCTGCGTTATCAGTTACCGGGACAATTTCGCTGTCAG
CCGCATTTTCGCGATAAGGGACGCGAGCTTAAAAAACTCTGGCAAGAATGCGCTGTGCCGCCATGGTTACGTGCTGAGGT
GGGGTTCTTGTTCTATAACGAGCGTTTAGTGATGGCGCTGGGACTCTGGGTCGAAAAGGCATTTTGTGCCCAAGGGGATG
ATGTCGGGCTTAAGTTTGATGGGCAAGCAATCGGATAG

Upstream 100 bases:

>100_bases
AGGTGCGGATCCTGTTCCCCTAATCGATAACCTGAAAGAGACTTTGGGGTTGGGGGATTATTGAGCATCAGCGAGTGATG
GATTTTATAGGGTGAGTGCA

Downstream 100 bases:

>100_bases
CAGAGTGTATTTGCCATGCTTTATCGCAAGTGAGCAGCATCCATGCTCGATTAAGATAGTTGGGCTGGACTTAACTTGTA
GCAATTACGACCCGAGGCTT

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MAAVELCAHIARFLASLPLQAGSKLVLAYSGGVDSEVLAYGLSEFAKHHPEFHYQLIYVHHGLSPNADAWAEHCQTRAAN
YGLPITVERVQLTLGPRVSIEAEARKMRYQAILQHLKPQDVLLTAHHEDDQLETILLALKRGQGPKGLAAMGQIQMLPLG
EKGACLQVRPLLDISREQIEAFAQTRQLVHIEDESNQDDKYDRNFLRLEIIPRLKARWPSIATTASRSAQLCAEQQAVVD
AEVSERLPKLLTEAHFTQQTVLKLDELATQTSEWQGLLLRGFIESQGFGLPSYVQLQQILQQLMGAKEDAKVQLQIGDCV
LRRFAGMLYLDTVNTVSAAPRITTRELHQDILALLTQASTRVEDKIAPFTLATTGPRLCLPKAGEVVSLRYQLPGQFRCQ
PHFRDKGRELKKLWQECAVPPWLRAEVGFLFYNERLVMALGLWVEKAFCAQGDDVGLKFDGQAIG

Sequences:

>Translated_465_residues
MAAVELCAHIARFLASLPLQAGSKLVLAYSGGVDSEVLAYGLSEFAKHHPEFHYQLIYVHHGLSPNADAWAEHCQTRAAN
YGLPITVERVQLTLGPRVSIEAEARKMRYQAILQHLKPQDVLLTAHHEDDQLETILLALKRGQGPKGLAAMGQIQMLPLG
EKGACLQVRPLLDISREQIEAFAQTRQLVHIEDESNQDDKYDRNFLRLEIIPRLKARWPSIATTASRSAQLCAEQQAVVD
AEVSERLPKLLTEAHFTQQTVLKLDELATQTSEWQGLLLRGFIESQGFGLPSYVQLQQILQQLMGAKEDAKVQLQIGDCV
LRRFAGMLYLDTVNTVSAAPRITTRELHQDILALLTQASTRVEDKIAPFTLATTGPRLCLPKAGEVVSLRYQLPGQFRCQ
PHFRDKGRELKKLWQECAVPPWLRAEVGFLFYNERLVMALGLWVEKAFCAQGDDVGLKFDGQAIG
>Mature_464_residues
AAVELCAHIARFLASLPLQAGSKLVLAYSGGVDSEVLAYGLSEFAKHHPEFHYQLIYVHHGLSPNADAWAEHCQTRAANY
GLPITVERVQLTLGPRVSIEAEARKMRYQAILQHLKPQDVLLTAHHEDDQLETILLALKRGQGPKGLAAMGQIQMLPLGE
KGACLQVRPLLDISREQIEAFAQTRQLVHIEDESNQDDKYDRNFLRLEIIPRLKARWPSIATTASRSAQLCAEQQAVVDA
EVSERLPKLLTEAHFTQQTVLKLDELATQTSEWQGLLLRGFIESQGFGLPSYVQLQQILQQLMGAKEDAKVQLQIGDCVL
RRFAGMLYLDTVNTVSAAPRITTRELHQDILALLTQASTRVEDKIAPFTLATTGPRLCLPKAGEVVSLRYQLPGQFRCQP
HFRDKGRELKKLWQECAVPPWLRAEVGFLFYNERLVMALGLWVEKAFCAQGDDVGLKFDGQAIG

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=441, Percent_Identity=37.6417233560091, Blast_Score=266, Evalue=3e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 51996; Mature: 51864

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAVELCAHIARFLASLPLQAGSKLVLAYSGGVDSEVLAYGLSEFAKHHPEFHYQLIYVH
CHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEEEE
HGLSPNADAWAEHCQTRAANYGLPITVERVQLTLGPRVSIEAEARKMRYQAILQHLKPQD
ECCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCEEEHHHHHHHHHHHHHHHCCCCC
VLLTAHHEDDQLETILLALKRGQGPKGLAAMGQIQMLPLGEKGACLQVRPLLDISREQIE
EEEEECCCCHHHHHHHHHHHCCCCCCCHHHCCCEEEEECCCCCCEEEECHHHHCCHHHHH
AFAQTRQLVHIEDESNQDDKYDRNFLRLEIIPRLKARWPSIATTASRSAQLCAEQQAVVD
HHHHHCCEEEEECCCCCCCCCCCCEEEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
AEVSERLPKLLTEAHFTQQTVLKLDELATQTSEWQGLLLRGFIESQGFGLPSYVQLQQIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
QQLMGAKEDAKVQLQIGDCVLRRFAGMLYLDTVNTVSAAPRITTRELHQDILALLTQAST
HHHHCCCCCCEEEEEHHHHHHHHHCCEEEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHH
RVEDKIAPFTLATTGPRLCLPKAGEVVSLRYQLPGQFRCQPHFRDKGRELKKLWQECAVP
HHHHCCCCEEEECCCCCEECCCCCCEEEEEEECCCCEECCCCHHHCCHHHHHHHHHHCCC
PWLRAEVGFLFYNERLVMALGLWVEKAFCAQGDDVGLKFDGQAIG
CCHHHHEEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCC
>Mature Secondary Structure 
AAVELCAHIARFLASLPLQAGSKLVLAYSGGVDSEVLAYGLSEFAKHHPEFHYQLIYVH
HHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEEEE
HGLSPNADAWAEHCQTRAANYGLPITVERVQLTLGPRVSIEAEARKMRYQAILQHLKPQD
ECCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCEEEHHHHHHHHHHHHHHHCCCCC
VLLTAHHEDDQLETILLALKRGQGPKGLAAMGQIQMLPLGEKGACLQVRPLLDISREQIE
EEEEECCCCHHHHHHHHHHHCCCCCCCHHHCCCEEEEECCCCCCEEEECHHHHCCHHHHH
AFAQTRQLVHIEDESNQDDKYDRNFLRLEIIPRLKARWPSIATTASRSAQLCAEQQAVVD
HHHHHCCEEEEECCCCCCCCCCCCEEEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
AEVSERLPKLLTEAHFTQQTVLKLDELATQTSEWQGLLLRGFIESQGFGLPSYVQLQQIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
QQLMGAKEDAKVQLQIGDCVLRRFAGMLYLDTVNTVSAAPRITTRELHQDILALLTQAST
HHHHCCCCCCEEEEEHHHHHHHHHCCEEEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHH
RVEDKIAPFTLATTGPRLCLPKAGEVVSLRYQLPGQFRCQPHFRDKGRELKKLWQECAVP
HHHHCCCCEEEECCCCCEECCCCCCEEEEEEECCCCEECCCCHHHCCHHHHHHHHHHCCC
PWLRAEVGFLFYNERLVMALGLWVEKAFCAQGDDVGLKFDGQAIG
CCHHHHEEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]