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The map label for this gene is kefB [H]

Identifier: 117921238

GI number: 117921238

Start: 3339625

End: 3341571

Strand: Reverse

Name: kefB [H]

Synonym: Shewana3_2797

Alternate gene names: 117921238

Gene position: 3341571-3339625 (Counterclockwise)

Preceding gene: 117921240

Following gene: 117921237

Centisome position: 67.21

GC content: 52.13

Gene sequence:

>1947_bases
ATGGAGCATGGTTTCTTGATCCAAGTTCTCCTTATGTTGCTGATCGCCATCGTCGCGATTGCCTTGCTGCGGCGTATCGG
CTTGCCGGCGATTTTGGCGTATTTATTAACCGGAGTGGTGAGTGGCCCCAGTGGATTCCACTGGTTTACCCAGCAGCAAA
TGCAGTCCGTCGCCGAACTTGGGGTTGTGCTCTTGATGTTTACGCTGGGACTCGAGTTTTCGGTGCCAAGATTGTGGGCC
ATGCGCAGAACCGTATTTGGATTAGGTAGCGCGCAGGTGATTGTCACCACAGTACTCACTATGTTGGTTGCCTTAGCCTG
TGGCTTGAATGGCATAGAATCTTTAGTTATAGGCGCAGCGATTGCCCTTTCCTCCACCGCGATTGTACTCAAGCTACTTA
ATGAGCAGGGCTGGCTTAGGCGCCGTCATGGCGAGCTGTCGGTCAGTGTGTTGTTATTTCAGGATTTAGCCGTCGTTCCT
CTGCTTATTTTGCTGCCATTACTCGGGCAGAATGATGAGCCCTTGATGTTAGCGACGATTACCTTGGCGCTACTTAAGGG
GATTCTTGCCTTCTTTTTACTGATGGCCTTAGGCAAATGGGCTTTGCCTCGCTTGTTTGATGAGGTGGCAAGATCCCGCT
CCAATGAACTCTTTGTACTCTCGACGCTGGTGGTGGCTTTAGTTACGGGAGCTTTTACCCAGTGGTTAGGTTTGTCTATG
GCGCTCGGCGCCTTTATGGCGGGAATGCTGCTCGGTGAGAGTCAATACCGCCGCCAGCTAGAGGCGGATATTCGGCCGTT
TCGCGATTTACTCATGGGGCTGTTTTTTATCTCCATCGGCATGATGCTCGATTTCGCACTGGTGATGCAATTCTGGTGGC
AGATTTTACTGATCTTGCTCGCAGTCGTGGTGGGCAAAGCGCTGATAGTCCATGGTTTATTGCGACTGGTCGGCGAGCCT
TTTCGGATTGCTATCAGCACGGCTTTGAGTCTGGCTCAGGTCGGCGAATTCAGTTTTGTGGTGTTAGCCCTTGCCGTAAG
TTATGGGTTATTGAGCAATCAGCTCAGTACTATGCTGGTGATGGTTGCGGTATTGTCTATGAGCATTGCCCCTTGGTTGG
TGCGCCATAGCGTAGATATTGCCAAGTGGCTGTTAGGGATCCGCCAGTCGGGGCATGTGGATGAAGTGGTGCCGATTATT
ACCGAGGACCATGATCTGGTGGTGATTTTAGGTTACGGACGTGTTGGTCAAACCATAGCCCGGTTTTTAAAGACAGAAGC
CGTGCCCTATTTGGTACTCGATCTTGACCCCACGCGGGTGTCTGAGGCGCGCCGGGCAGGGGAGCCGATTTACTTTGGCG
ATGTGTGTAAGCGGGCAATCCTTAAGCAGGTAGGGATTAAACACGCCAAAATGATCGTTATTACCTTCTGCGAAACCCGC
AGTTTAGAAGAAGCCTTACCCCTGTGTAAGCAATTGGCGCCCGACGCTAAAATTCTGGTCAGAACCCGCGATGACAGTGA
GTTAGACATGCTGCAAAAGGCGGGGGCGAATCAGGTGATCCCCGAAACTTTAGAGGGCAGTTTGATGTTGGTCTCGCAGG
TGCTGCATCAATGTGGTGTACCGTTGGCGCGGATCCTTAAGCGACTGGAGTCGGAGCGGCGCAACCATTATCAATTTTTA
CACGGTTTCTTCTCGGGTACCGAAACTGACTTTACCTTAGAGTCGCTCCATGCCGTGCTGTTACACCGCGGCGCGGATGC
CGTGGGTAAGCAAGTGGCGGATATTGACTGGGAGCTGCTTCGGGTAGAGTTAAGGGCCATCCGTCGCAGCGGCGCCGAAG
TTGAACATCCTGCGCAGGATTGGGTCTTTCGCGCGGGTGATATTTTACTGATTGTGGGTAAGCCCAGAAGGCTTGAAAAA
GCCGAGGCTAAGCTATTGCATGGATAG

Upstream 100 bases:

>100_bases
GTTTGCTAATAAAATTTGTCTTTGTATGTCACTCAGTTAAGCTTAAAGCGCACTCCCATATTTTTGACACGCCAGTTATT
TGACAGGAGTTATCGAGCCT

Downstream 100 bases:

>100_bases
CTGTATGCTTGCTTAGGTAGTGACATCACGGCTTGCAAAAGTAGCCGCAATCCTAACTTATCAATGTGCTCGTTGCGCAT
TGTGTTAACCGTTCGCTATC

Product: potassium efflux system protein

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 648; Mature: 648

Protein sequence:

>648_residues
MEHGFLIQVLLMLLIAIVAIALLRRIGLPAILAYLLTGVVSGPSGFHWFTQQQMQSVAELGVVLLMFTLGLEFSVPRLWA
MRRTVFGLGSAQVIVTTVLTMLVALACGLNGIESLVIGAAIALSSTAIVLKLLNEQGWLRRRHGELSVSVLLFQDLAVVP
LLILLPLLGQNDEPLMLATITLALLKGILAFFLLMALGKWALPRLFDEVARSRSNELFVLSTLVVALVTGAFTQWLGLSM
ALGAFMAGMLLGESQYRRQLEADIRPFRDLLMGLFFISIGMMLDFALVMQFWWQILLILLAVVVGKALIVHGLLRLVGEP
FRIAISTALSLAQVGEFSFVVLALAVSYGLLSNQLSTMLVMVAVLSMSIAPWLVRHSVDIAKWLLGIRQSGHVDEVVPII
TEDHDLVVILGYGRVGQTIARFLKTEAVPYLVLDLDPTRVSEARRAGEPIYFGDVCKRAILKQVGIKHAKMIVITFCETR
SLEEALPLCKQLAPDAKILVRTRDDSELDMLQKAGANQVIPETLEGSLMLVSQVLHQCGVPLARILKRLESERRNHYQFL
HGFFSGTETDFTLESLHAVLLHRGADAVGKQVADIDWELLRVELRAIRRSGAEVEHPAQDWVFRAGDILLIVGKPRRLEK
AEAKLLHG

Sequences:

>Translated_648_residues
MEHGFLIQVLLMLLIAIVAIALLRRIGLPAILAYLLTGVVSGPSGFHWFTQQQMQSVAELGVVLLMFTLGLEFSVPRLWA
MRRTVFGLGSAQVIVTTVLTMLVALACGLNGIESLVIGAAIALSSTAIVLKLLNEQGWLRRRHGELSVSVLLFQDLAVVP
LLILLPLLGQNDEPLMLATITLALLKGILAFFLLMALGKWALPRLFDEVARSRSNELFVLSTLVVALVTGAFTQWLGLSM
ALGAFMAGMLLGESQYRRQLEADIRPFRDLLMGLFFISIGMMLDFALVMQFWWQILLILLAVVVGKALIVHGLLRLVGEP
FRIAISTALSLAQVGEFSFVVLALAVSYGLLSNQLSTMLVMVAVLSMSIAPWLVRHSVDIAKWLLGIRQSGHVDEVVPII
TEDHDLVVILGYGRVGQTIARFLKTEAVPYLVLDLDPTRVSEARRAGEPIYFGDVCKRAILKQVGIKHAKMIVITFCETR
SLEEALPLCKQLAPDAKILVRTRDDSELDMLQKAGANQVIPETLEGSLMLVSQVLHQCGVPLARILKRLESERRNHYQFL
HGFFSGTETDFTLESLHAVLLHRGADAVGKQVADIDWELLRVELRAIRRSGAEVEHPAQDWVFRAGDILLIVGKPRRLEK
AEAKLLHG
>Mature_648_residues
MEHGFLIQVLLMLLIAIVAIALLRRIGLPAILAYLLTGVVSGPSGFHWFTQQQMQSVAELGVVLLMFTLGLEFSVPRLWA
MRRTVFGLGSAQVIVTTVLTMLVALACGLNGIESLVIGAAIALSSTAIVLKLLNEQGWLRRRHGELSVSVLLFQDLAVVP
LLILLPLLGQNDEPLMLATITLALLKGILAFFLLMALGKWALPRLFDEVARSRSNELFVLSTLVVALVTGAFTQWLGLSM
ALGAFMAGMLLGESQYRRQLEADIRPFRDLLMGLFFISIGMMLDFALVMQFWWQILLILLAVVVGKALIVHGLLRLVGEP
FRIAISTALSLAQVGEFSFVVLALAVSYGLLSNQLSTMLVMVAVLSMSIAPWLVRHSVDIAKWLLGIRQSGHVDEVVPII
TEDHDLVVILGYGRVGQTIARFLKTEAVPYLVLDLDPTRVSEARRAGEPIYFGDVCKRAILKQVGIKHAKMIVITFCETR
SLEEALPLCKQLAPDAKILVRTRDDSELDMLQKAGANQVIPETLEGSLMLVSQVLHQCGVPLARILKRLESERRNHYQFL
HGFFSGTETDFTLESLHAVLLHRGADAVGKQVADIDWELLRVELRAIRRSGAEVEHPAQDWVFRAGDILLIVGKPRRLEK
AEAKLLHG

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=405, Percent_Identity=27.6543209876543, Blast_Score=94, Evalue=4e-19,
Organism=Escherichia coli, GI1789749, Length=538, Percent_Identity=35.8736059479554, Blast_Score=287, Evalue=1e-78,
Organism=Escherichia coli, GI1786232, Length=573, Percent_Identity=31.9371727748691, Blast_Score=268, Evalue=6e-73,
Organism=Escherichia coli, GI1786685, Length=525, Percent_Identity=31.2380952380952, Blast_Score=203, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6322367, Length=529, Percent_Identity=22.117202268431, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 71515; Mature: 71515

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHGFLIQVLLMLLIAIVAIALLRRIGLPAILAYLLTGVVSGPSGFHWFTQQQMQSVAEL
CCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
GVVLLMFTLGLEFSVPRLWAMRRTVFGLGSAQVIVTTVLTMLVALACGLNGIESLVIGAA
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
IALSSTAIVLKLLNEQGWLRRRHGELSVSVLLFQDLAVVPLLILLPLLGQNDEPLMLATI
HHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
TLALLKGILAFFLLMALGKWALPRLFDEVARSRSNELFVLSTLVVALVTGAFTQWLGLSM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
ALGAFMAGMLLGESQYRRQLEADIRPFRDLLMGLFFISIGMMLDFALVMQFWWQILLILL
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVVVGKALIVHGLLRLVGEPFRIAISTALSLAQVGEFSFVVLALAVSYGLLSNQLSTMLV
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
MVAVLSMSIAPWLVRHSVDIAKWLLGIRQSGHVDEVVPIITEDHDLVVILGYGRVGQTIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHEECCCCEEEEEECCHHHHHHH
RFLKTEAVPYLVLDLDPTRVSEARRAGEPIYFGDVCKRAILKQVGIKHAKMIVITFCETR
HHHHHCCCCEEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHEEEEEECCCC
SLEEALPLCKQLAPDAKILVRTRDDSELDMLQKAGANQVIPETLEGSLMLVSQVLHQCGV
CHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHCCC
PLARILKRLESERRNHYQFLHGFFSGTETDFTLESLHAVLLHRGADAVGKQVADIDWELL
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCHHHHH
RVELRAIRRSGAEVEHPAQDWVFRAGDILLIVGKPRRLEKAEAKLLHG
HHHHHHHHHCCCCCCCCHHHHHEECCCEEEEECCCCHHHHHHHHHCCC
>Mature Secondary Structure
MEHGFLIQVLLMLLIAIVAIALLRRIGLPAILAYLLTGVVSGPSGFHWFTQQQMQSVAEL
CCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
GVVLLMFTLGLEFSVPRLWAMRRTVFGLGSAQVIVTTVLTMLVALACGLNGIESLVIGAA
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
IALSSTAIVLKLLNEQGWLRRRHGELSVSVLLFQDLAVVPLLILLPLLGQNDEPLMLATI
HHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
TLALLKGILAFFLLMALGKWALPRLFDEVARSRSNELFVLSTLVVALVTGAFTQWLGLSM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
ALGAFMAGMLLGESQYRRQLEADIRPFRDLLMGLFFISIGMMLDFALVMQFWWQILLILL
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVVVGKALIVHGLLRLVGEPFRIAISTALSLAQVGEFSFVVLALAVSYGLLSNQLSTMLV
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
MVAVLSMSIAPWLVRHSVDIAKWLLGIRQSGHVDEVVPIITEDHDLVVILGYGRVGQTIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHEECCCCEEEEEECCHHHHHHH
RFLKTEAVPYLVLDLDPTRVSEARRAGEPIYFGDVCKRAILKQVGIKHAKMIVITFCETR
HHHHHCCCCEEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHEEEEEECCCC
SLEEALPLCKQLAPDAKILVRTRDDSELDMLQKAGANQVIPETLEGSLMLVSQVLHQCGV
CHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHCCC
PLARILKRLESERRNHYQFLHGFFSGTETDFTLESLHAVLLHRGADAVGKQVADIDWELL
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCHHHHH
RVELRAIRRSGAEVEHPAQDWVFRAGDILLIVGKPRRLEKAEAKLLHG
HHHHHHHHHCCCCCCCCHHHHHEECCCEEEEECCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA