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The map label for this gene is mmsB [H]

Identifier: 117921207

GI number: 117921207

Start: 3304349

End: 3305251

Strand: Reverse

Name: mmsB [H]

Synonym: Shewana3_2766

Alternate gene names: 117921207

Gene position: 3305251-3304349 (Counterclockwise)

Preceding gene: 117921208

Following gene: 117921206

Centisome position: 66.47

GC content: 51.72

Gene sequence:

>903_bases
ATGAGTACTGTAGCATTTATCGGTTTAGGTAATATGGGCGGCCCAATGGCGGCCAATTTATTAAAAGCGGGCATGACGGT
ACGTGTCTTCGACTTAGTGCCAGCCGCCATGCAAGCCTTGGCCGACCAAGGCGCCTTAGTGTCGAGCACGGCATGCGGCG
CTGCGGCGGGAGCCAATGTGGTGATCACTATGCTGCCTGCGGGTAAGCATGTTAGAAATTTATATTTAGGTAATGGCAGC
GAGAAAGGCCTACTCGAGGTAGTCGCTGGCGATACTTTGTTGATTGATTGCTCGACCATTGATGCCCAAAGCGCCCAATT
GGTGGCGGCCGAAGCGGCTAAGAGTGGTATCGAATTTATCGATGCGCCAGTATCGGGTGGTACGGCGGGCGCGGCGGCAG
GCACCTTGACCTTTATCTGTGGAGGCTCGGACATCGCCTTCGAACGTGCGCAACCTGTACTTAACGCTATGGGCAAGAAT
ATCTTCCATGCGGGCGGCGCTGGTGCCGGTCAGGTCGCTAAAATCTGCAACAACATGCTGCTATCTGTGTTGATGGTCGG
TACTTCTGAGGCATTGCAAATGGGGATTGACCATGGTCTCGATCCTAAAGTGCTGTCGGACATTATGAAGGTCAGCAGCG
GCGGTAACTGGACCTTAGAGAAGTACAATCCTTGCCCGGGCGTAATGGAGAATGTGCCTTCTTCTAAGGGCTATCAGGGC
GGCTTTATGGTAGATTTGATGGTTAAAGATCTCGGCCTCTCTCAAGAAGCAGCATTATTGAGCAATTCGAGTACACCCAT
GGGCTCACTCGCGCGCAGCTTGTATGTGAATCATGCAAGACAAGGCAATGGTCGTCGAGACTTCTCCAGTATTTTTGAAC
AATTTGCACCACTTAAAAAATAG

Upstream 100 bases:

>100_bases
GCCAATCGATGGCAGGTAAAGCGAGCGCTTTGCCTGCCATTTTTGTCTGGAATAACAACTATCTGGAATTATAAAAACAC
CAGAACACAGGAGTGATGGC

Downstream 100 bases:

>100_bases
ATAAACAAGGGATAACCAGATGGATTTAAAAGATAAGGTTGTCGTCATCACTGGCGGCGCGGGTGGTTTAGGCTTAGCAA
TGGCGCACAATTTTGCGCAG

Product: 3-hydroxyisobutyrate dehydrogenase

Products: NA

Alternate protein names: HIBADH [H]

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MSTVAFIGLGNMGGPMAANLLKAGMTVRVFDLVPAAMQALADQGALVSSTACGAAAGANVVITMLPAGKHVRNLYLGNGS
EKGLLEVVAGDTLLIDCSTIDAQSAQLVAAEAAKSGIEFIDAPVSGGTAGAAAGTLTFICGGSDIAFERAQPVLNAMGKN
IFHAGGAGAGQVAKICNNMLLSVLMVGTSEALQMGIDHGLDPKVLSDIMKVSSGGNWTLEKYNPCPGVMENVPSSKGYQG
GFMVDLMVKDLGLSQEAALLSNSSTPMGSLARSLYVNHARQGNGRRDFSSIFEQFAPLKK

Sequences:

>Translated_300_residues
MSTVAFIGLGNMGGPMAANLLKAGMTVRVFDLVPAAMQALADQGALVSSTACGAAAGANVVITMLPAGKHVRNLYLGNGS
EKGLLEVVAGDTLLIDCSTIDAQSAQLVAAEAAKSGIEFIDAPVSGGTAGAAAGTLTFICGGSDIAFERAQPVLNAMGKN
IFHAGGAGAGQVAKICNNMLLSVLMVGTSEALQMGIDHGLDPKVLSDIMKVSSGGNWTLEKYNPCPGVMENVPSSKGYQG
GFMVDLMVKDLGLSQEAALLSNSSTPMGSLARSLYVNHARQGNGRRDFSSIFEQFAPLKK
>Mature_299_residues
STVAFIGLGNMGGPMAANLLKAGMTVRVFDLVPAAMQALADQGALVSSTACGAAAGANVVITMLPAGKHVRNLYLGNGSE
KGLLEVVAGDTLLIDCSTIDAQSAQLVAAEAAKSGIEFIDAPVSGGTAGAAAGTLTFICGGSDIAFERAQPVLNAMGKNI
FHAGGAGAGQVAKICNNMLLSVLMVGTSEALQMGIDHGLDPKVLSDIMKVSSGGNWTLEKYNPCPGVMENVPSSKGYQGG
FMVDLMVKDLGLSQEAALLSNSSTPMGSLARSLYVNHARQGNGRRDFSSIFEQFAPLKK

Specific function: Unknown

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=292, Percent_Identity=46.5753424657534, Blast_Score=261, Evalue=5e-70,
Organism=Homo sapiens, GI40556376, Length=292, Percent_Identity=24.3150684931507, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1790315, Length=295, Percent_Identity=37.6271186440678, Blast_Score=164, Evalue=5e-42,
Organism=Escherichia coli, GI145693186, Length=299, Percent_Identity=32.1070234113712, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1786719, Length=289, Percent_Identity=31.1418685121107, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI1789092, Length=295, Percent_Identity=30.5084745762712, Blast_Score=112, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17557316, Length=302, Percent_Identity=46.3576158940397, Blast_Score=243, Evalue=6e-65,
Organism=Drosophila melanogaster, GI24655230, Length=290, Percent_Identity=42.0689655172414, Blast_Score=228, Evalue=5e-60,
Organism=Drosophila melanogaster, GI19922568, Length=290, Percent_Identity=42.0689655172414, Blast_Score=228, Evalue=5e-60,
Organism=Drosophila melanogaster, GI28574115, Length=292, Percent_Identity=23.972602739726, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR011548
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: =1.1.1.31 [H]

Molecular weight: Translated: 30671; Mature: 30540

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTVAFIGLGNMGGPMAANLLKAGMTVRVFDLVPAAMQALADQGALVSSTACGAAAGANV
CCEEEEEEECCCCCHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCEEECCCCCCCCCCCE
VITMLPAGKHVRNLYLGNGSEKGLLEVVAGDTLLIDCSTIDAQSAQLVAAEAAKSGIEFI
EEEEECCCCHHHEEEECCCCCCCEEEEEECCEEEEEECCCCCCCHHHHHHHHHHCCCCEE
DAPVSGGTAGAAAGTLTFICGGSDIAFERAQPVLNAMGKNIFHAGGAGAGQVAKICNNML
ECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCHHEECCCCCHHHHHHHHHHHH
LSVLMVGTSEALQMGIDHGLDPKVLSDIMKVSSGGNWTLEKYNPCPGVMENVPSSKGYQG
HHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCHHHCCCCCCCCCC
GFMVDLMVKDLGLSQEAALLSNSSTPMGSLARSLYVNHARQGNGRRDFSSIFEQFAPLKK
CEEEEEHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
STVAFIGLGNMGGPMAANLLKAGMTVRVFDLVPAAMQALADQGALVSSTACGAAAGANV
CEEEEEEECCCCCHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCEEECCCCCCCCCCCE
VITMLPAGKHVRNLYLGNGSEKGLLEVVAGDTLLIDCSTIDAQSAQLVAAEAAKSGIEFI
EEEEECCCCHHHEEEECCCCCCCEEEEEECCEEEEEECCCCCCCHHHHHHHHHHCCCCEE
DAPVSGGTAGAAAGTLTFICGGSDIAFERAQPVLNAMGKNIFHAGGAGAGQVAKICNNML
ECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCHHEECCCCCHHHHHHHHHHHH
LSVLMVGTSEALQMGIDHGLDPKVLSDIMKVSSGGNWTLEKYNPCPGVMENVPSSKGYQG
HHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCHHHCCCCCCCCCC
GFMVDLMVKDLGLSQEAALLSNSSTPMGSLARSLYVNHARQGNGRRDFSSIFEQFAPLKK
CEEEEEHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1339433; 10984043 [H]