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The map label for this gene is yngF [H]

Identifier: 117921208

GI number: 117921208

Start: 3305352

End: 3306503

Strand: Reverse

Name: yngF [H]

Synonym: Shewana3_2767

Alternate gene names: 117921208

Gene position: 3306503-3305352 (Counterclockwise)

Preceding gene: 117921209

Following gene: 117921207

Centisome position: 66.5

GC content: 50.78

Gene sequence:

>1152_bases
ATGACAAATTTAGTAGATAAGGCTGCACACACGTCCACCACACAAAATGTGGTTTTTCAAACCTTAGCCACCGCCTCAGG
TAAATTAGTTGGGGTGGTGACGCTCAATGTTGAAAAAGCCCTCAACGCCTTGGATCTCGATATGGTTCGAGCCATGACGG
TGCAGCTCAATTTGTGGAAAAAAGATCCGTTAATCGCCTGTGTGGTACTCGATGGCAGCGGAGAAAAGGCGTTCTGTGCG
GGTGGCGATGTGCGCGCGTTATACCACGCGTCTGTTGCCGCTAAGGGACAAGTGACTGAGGTCGCTAAAGTCTTCTTCGA
AGAAGAATATCGTCTCGATTATCTACTGCATACCTATGGCAAACCTGTGCTGGTATGGGGAGATGGCATAGTGATGGGCG
GCGGTCTGGGTCTGATGGCAGGCGCGAGTCATAGAGTCGTGACAGAAACCTCCCGTATCGCCATGCCCGAAGTCACCATC
GGGCTTTATCCCGATGTGGGCGGCAGTTATTTCCTCAACCGTATGCCGGGTAAAATGGGGCTGTTTTTAGGGCTAACGGC
CTATCAAATGAACGCCGCCGATGCCTGTTACGTTGGCCTTGCCGATCATTATCTGAATCGTGACGACAAAGAGCTGATGT
TCGATGCCATGGCTACCCTCGATTGGAGCGATACGCCAGCGCTGAACCATCAAAGACTCGATACGATGATCAACGAGCTA
TCGAACCAAGTGGACATCCCTAAGGGCGATAGTTTACTGGCCGAGAGCCAAGAGATGATCGACCGACTGATGGCGGGCAG
CTTGACGGATATCGTCAGCCGTATGTCAACCTTAAGCACCGAAGAAGCCTGGTTAAATAAAGCCTGCGCGACTATGCTCG
CGGGCAGCCCTATCAGTTGGCATTTGGCGTATATCCAAACGCAACTCGGCACCAAACTTAGCCTAGCACAATGTTTTAAA
TGGGAATTAACCGTCAGCGTTAACACCTGTGCTAAGGGCGATTTCTGTGAAGGCGTACGGGCACTGCTGATTGATAAAGA
CAGACAGCCAAAATGGCAATTTGCCGATGTGCAATCCGTGCCAAACAGCGTTATCGAGGACATTTTGACCTCACCTTGGG
GAGAGGAACATCCCTTAAGCCAATTAAGCTAA

Upstream 100 bases:

>100_bases
TAGAGCGTGAACTGTTTGTCGGATTGTTCGATACTGAAGATCAGGCCGAAGGCGTGAACGCGTTTTTGGAAAAACGTAAA
GCTGATTGGAAGAACCGCTA

Downstream 100 bases:

>100_bases
GCCAATCGATGGCAGGTAAAGCGAGCGCTTTGCCTGCCATTTTTGTCTGGAATAACAACTATCTGGAATTATAAAAACAC
CAGAACACAGGAGTGATGGC

Product: enoyl-CoA hydratase/isomerase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MTNLVDKAAHTSTTQNVVFQTLATASGKLVGVVTLNVEKALNALDLDMVRAMTVQLNLWKKDPLIACVVLDGSGEKAFCA
GGDVRALYHASVAAKGQVTEVAKVFFEEEYRLDYLLHTYGKPVLVWGDGIVMGGGLGLMAGASHRVVTETSRIAMPEVTI
GLYPDVGGSYFLNRMPGKMGLFLGLTAYQMNAADACYVGLADHYLNRDDKELMFDAMATLDWSDTPALNHQRLDTMINEL
SNQVDIPKGDSLLAESQEMIDRLMAGSLTDIVSRMSTLSTEEAWLNKACATMLAGSPISWHLAYIQTQLGTKLSLAQCFK
WELTVSVNTCAKGDFCEGVRALLIDKDRQPKWQFADVQSVPNSVIEDILTSPWGEEHPLSQLS

Sequences:

>Translated_383_residues
MTNLVDKAAHTSTTQNVVFQTLATASGKLVGVVTLNVEKALNALDLDMVRAMTVQLNLWKKDPLIACVVLDGSGEKAFCA
GGDVRALYHASVAAKGQVTEVAKVFFEEEYRLDYLLHTYGKPVLVWGDGIVMGGGLGLMAGASHRVVTETSRIAMPEVTI
GLYPDVGGSYFLNRMPGKMGLFLGLTAYQMNAADACYVGLADHYLNRDDKELMFDAMATLDWSDTPALNHQRLDTMINEL
SNQVDIPKGDSLLAESQEMIDRLMAGSLTDIVSRMSTLSTEEAWLNKACATMLAGSPISWHLAYIQTQLGTKLSLAQCFK
WELTVSVNTCAKGDFCEGVRALLIDKDRQPKWQFADVQSVPNSVIEDILTSPWGEEHPLSQLS
>Mature_382_residues
TNLVDKAAHTSTTQNVVFQTLATASGKLVGVVTLNVEKALNALDLDMVRAMTVQLNLWKKDPLIACVVLDGSGEKAFCAG
GDVRALYHASVAAKGQVTEVAKVFFEEEYRLDYLLHTYGKPVLVWGDGIVMGGGLGLMAGASHRVVTETSRIAMPEVTIG
LYPDVGGSYFLNRMPGKMGLFLGLTAYQMNAADACYVGLADHYLNRDDKELMFDAMATLDWSDTPALNHQRLDTMINELS
NQVDIPKGDSLLAESQEMIDRLMAGSLTDIVSRMSTLSTEEAWLNKACATMLAGSPISWHLAYIQTQLGTKLSLAQCFKW
ELTVSVNTCAKGDFCEGVRALLIDKDRQPKWQFADVQSVPNSVIEDILTSPWGEEHPLSQLS

Specific function: Catalyzes The Reversible Dehydration Of L-Carnitinyl-CoA To Crotobetainyl-CoA. [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI37594471, Length=363, Percent_Identity=34.9862258953168, Blast_Score=206, Evalue=3e-53,
Organism=Homo sapiens, GI37594469, Length=328, Percent_Identity=32.6219512195122, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI194097323, Length=177, Percent_Identity=31.638418079096, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI25144157, Length=340, Percent_Identity=38.8235294117647, Blast_Score=228, Evalue=5e-60,
Organism=Caenorhabditis elegans, GI25144160, Length=340, Percent_Identity=38.8235294117647, Blast_Score=228, Evalue=6e-60,
Organism=Caenorhabditis elegans, GI17540714, Length=203, Percent_Identity=28.0788177339901, Blast_Score=65, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI25145438, Length=183, Percent_Identity=30.0546448087432, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=348, Percent_Identity=32.7586206896552, Blast_Score=155, Evalue=7e-39,
Organism=Drosophila melanogaster, GI28571730, Length=341, Percent_Identity=40.4692082111437, Blast_Score=227, Evalue=1e-59,
Organism=Drosophila melanogaster, GI28571729, Length=341, Percent_Identity=40.4692082111437, Blast_Score=227, Evalue=1e-59,
Organism=Drosophila melanogaster, GI20129971, Length=208, Percent_Identity=30.2884615384615, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24653477, Length=208, Percent_Identity=30.2884615384615, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 41806; Mature: 41675

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLVDKAAHTSTTQNVVFQTLATASGKLVGVVTLNVEKALNALDLDMVRAMTVQLNLWK
CCCHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEEEEHHHHHHHHHHHHHHEEEEEEEEEC
KDPLIACVVLDGSGEKAFCAGGDVRALYHASVAAKGQVTEVAKVFFEEEYRLDYLLHTYG
CCCEEEEEEEECCCCEEEECCCCEEEHEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC
KPVLVWGDGIVMGGGLGLMAGASHRVVTETSRIAMPEVTIGLYPDVGGSYFLNRMPGKMG
CEEEEECCCEEEECCCHHHCCCCCCEEECCCCEECCEEEEEECCCCCCHHHHHCCCCCHH
LFLGLTAYQMNAADACYVGLADHYLNRDDKELMFDAMATLDWSDTPALNHQRLDTMINEL
HEEEEEHHCCCCCCEEEEEHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
SNQVDIPKGDSLLAESQEMIDRLMAGSLTDIVSRMSTLSTEEAWLNKACATMLAGSPISW
HCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEE
HLAYIQTQLGTKLSLAQCFKWELTVSVNTCAKGDFCEGVRALLIDKDRQPKWQFADVQSV
EHHHHHHHHCCCCCHHHHEEEEEEEEEECCCCCCHHHHHHHHEECCCCCCCCEEHHHHHC
PNSVIEDILTSPWGEEHPLSQLS
HHHHHHHHHHCCCCCCCCHHHCC
>Mature Secondary Structure 
TNLVDKAAHTSTTQNVVFQTLATASGKLVGVVTLNVEKALNALDLDMVRAMTVQLNLWK
CCHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEEEEHHHHHHHHHHHHHHEEEEEEEEEC
KDPLIACVVLDGSGEKAFCAGGDVRALYHASVAAKGQVTEVAKVFFEEEYRLDYLLHTYG
CCCEEEEEEEECCCCEEEECCCCEEEHEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC
KPVLVWGDGIVMGGGLGLMAGASHRVVTETSRIAMPEVTIGLYPDVGGSYFLNRMPGKMG
CEEEEECCCEEEECCCHHHCCCCCCEEECCCCEECCEEEEEECCCCCCHHHHHCCCCCHH
LFLGLTAYQMNAADACYVGLADHYLNRDDKELMFDAMATLDWSDTPALNHQRLDTMINEL
HEEEEEHHCCCCCCEEEEEHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
SNQVDIPKGDSLLAESQEMIDRLMAGSLTDIVSRMSTLSTEEAWLNKACATMLAGSPISW
HCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEE
HLAYIQTQLGTKLSLAQCFKWELTVSVNTCAKGDFCEGVRALLIDKDRQPKWQFADVQSV
EHHHHHHHHCCCCCHHHHEEEEEEEEEECCCCCCHHHHHHHHEECCCCCCCCEEHHHHHC
PNSVIEDILTSPWGEEHPLSQLS
HHHHHHHHHHCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]