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The map label for this gene is ybjS [C]

Identifier: 117921153

GI number: 117921153

Start: 3241383

End: 3242567

Strand: Reverse

Name: ybjS [C]

Synonym: Shewana3_2712

Alternate gene names: 117921153

Gene position: 3242567-3241383 (Counterclockwise)

Preceding gene: 117921154

Following gene: 117921152

Centisome position: 65.21

GC content: 52.15

Gene sequence:

>1185_bases
TTGCACTTAATCACTGAATTGAATACTAAGATGACACTCACTGTCACGGAATGCGATATGACTGACAATCCTGTTGTTCT
TCAAGCCGATACTACGCCAACTGGCCGCCCTGATAACCTCGCTCAAGCAGGCTTTGATCCGCGTGAACAAGCCGCGCTTT
ATGCCTTGGCGGCTAAGGTCAGCCATGCCTTTGTGACTGGCGCGGGTGGATTTTTGGGTAAGGCCATTTGCCTACGTTTG
GTCGCGGCTGGGATTAAGGTTACTGGGTTTGCCCGTGGCCATTACCCTGAGCTTGAAGCCCTTGGTGTGACTATGGTGCA
GGGGGATTTAGCCTATCCTGAGCAAGTCAAACAGGCGATGCAGGGTTGCGATATCGTGTTTCATGTGGCCTCAAAAGCGG
GTGTCTGGGGCGACAGAGACAGTTATTTTTGCCCCAATGTGAAGGGCGCCGCTAATGTGATTGCCGCTTGTAAAGCGTTT
AAAATCAGTAAACTAGTTTATACCAGCACTCCTAGCGTGACCTTTGCGGGTGAGGATGAATCGGGTATCGATGAGTCGAC
GCCCTACGCCAGCCGCTTTTTAAATTATTATGCTCACTCCAAGGCGATTGCCGAGAAGATGATGCTCGATGCCAATCAAG
CATCGATAACCGCGTCGGCGTATGCGTTAAAGACAGTTGCCTTGCGGCCGCATTTGATTTGGGGGCCTAATGATCCGCAT
CTGGTGCCTCGGGTGCTCGCCCGCGGTCGTTTAGGTAAGCTCAAGCTGGTGGGGCGCGAGGATAAACTGGTCGATACCAT
TTATATCGATAACGCCGCCTATGCCCATATTTTGGCGGCCGTGGAGCTTTGCCAAGTCTCGCCTAAGTGCCAAGGCAAAG
CCTATTTTGTCAGTAACGATGAGCCTGTGACTATGGCCAAGATGCTGAACCTCATCCTAGCCTGTGATGGCCTCCCGCCT
GTGACCGCGCGAGTGCCACAGACCCTAGCCTATGTGGCGGGCGCTGTGTTAGAAACGGCGTATCGACTGCTGAATAAACA
AGAAGAGCCGATAATGACGCGCTTTGTCGCTAAACAGCTTTCCTGTAGCCATTATTTCGATATCAGCGCGGCAAAACGGG
ACTTTGGCTATCACGCCTTAGTGTCGATAGAAGAGGGGATGAAGCGCCTTAAGGCATCACTCTAG

Upstream 100 bases:

>100_bases
CAAACCAAAGGCTGATTTCAATTTAGCTAATTTTTAACTTAAATAGGGTCGAACTTTCGACTGTTTGTTCGTGGTATGTT
TTGCCGCGAGAGGTGCCTTG

Downstream 100 bases:

>100_bases
TGCTTATTTAGCACAGGGCAAACTTGGTGATATCCTATGCCGTCACGGTAACTTACTGGATTAAAAAGTTAATGGCCTGC
GACGATTGTAATACTTCGAT

Product: 3-beta hydroxysteroid dehydrogenase/isomerase

Products: NA

Alternate protein names: Cholesterol dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3-beta-HSD; 3BHSD; 3-beta hydroxysterol dehydrogenase; 3-beta-hydroxy-5-ene steroid dehydrogenase; Progesterone reductase; Steroid Delta-isomerase; Delta-5-3-ketosteroid isomerase [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MHLITELNTKMTLTVTECDMTDNPVVLQADTTPTGRPDNLAQAGFDPREQAALYALAAKVSHAFVTGAGGFLGKAICLRL
VAAGIKVTGFARGHYPELEALGVTMVQGDLAYPEQVKQAMQGCDIVFHVASKAGVWGDRDSYFCPNVKGAANVIAACKAF
KISKLVYTSTPSVTFAGEDESGIDESTPYASRFLNYYAHSKAIAEKMMLDANQASITASAYALKTVALRPHLIWGPNDPH
LVPRVLARGRLGKLKLVGREDKLVDTIYIDNAAYAHILAAVELCQVSPKCQGKAYFVSNDEPVTMAKMLNLILACDGLPP
VTARVPQTLAYVAGAVLETAYRLLNKQEEPIMTRFVAKQLSCSHYFDISAAKRDFGYHALVSIEEGMKRLKASL

Sequences:

>Translated_394_residues
MHLITELNTKMTLTVTECDMTDNPVVLQADTTPTGRPDNLAQAGFDPREQAALYALAAKVSHAFVTGAGGFLGKAICLRL
VAAGIKVTGFARGHYPELEALGVTMVQGDLAYPEQVKQAMQGCDIVFHVASKAGVWGDRDSYFCPNVKGAANVIAACKAF
KISKLVYTSTPSVTFAGEDESGIDESTPYASRFLNYYAHSKAIAEKMMLDANQASITASAYALKTVALRPHLIWGPNDPH
LVPRVLARGRLGKLKLVGREDKLVDTIYIDNAAYAHILAAVELCQVSPKCQGKAYFVSNDEPVTMAKMLNLILACDGLPP
VTARVPQTLAYVAGAVLETAYRLLNKQEEPIMTRFVAKQLSCSHYFDISAAKRDFGYHALVSIEEGMKRLKASL
>Mature_394_residues
MHLITELNTKMTLTVTECDMTDNPVVLQADTTPTGRPDNLAQAGFDPREQAALYALAAKVSHAFVTGAGGFLGKAICLRL
VAAGIKVTGFARGHYPELEALGVTMVQGDLAYPEQVKQAMQGCDIVFHVASKAGVWGDRDSYFCPNVKGAANVIAACKAF
KISKLVYTSTPSVTFAGEDESGIDESTPYASRFLNYYAHSKAIAEKMMLDANQASITASAYALKTVALRPHLIWGPNDPH
LVPRVLARGRLGKLKLVGREDKLVDTIYIDNAAYAHILAAVELCQVSPKCQGKAYFVSNDEPVTMAKMLNLILACDGLPP
VTARVPQTLAYVAGAVLETAYRLLNKQEEPIMTRFVAKQLSCSHYFDISAAKRDFGYHALVSIEEGMKRLKASL

Specific function: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidation and isomerization of cholesterol, pregnenolone, and dehydroepiandrosterone (DHEA) into cholest-4-en-3-one, progesterone, and androsterone, respectively [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-beta-HSD family [H]

Homologues:

Organism=Homo sapiens, GI193211614, Length=333, Percent_Identity=32.1321321321321, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI8393516, Length=333, Percent_Identity=32.1321321321321, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI116268111, Length=341, Percent_Identity=30.791788856305, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI239745448, Length=348, Percent_Identity=29.3103448275862, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI310132178, Length=348, Percent_Identity=29.3103448275862, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI310113012, Length=348, Percent_Identity=29.3103448275862, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI260763931, Length=351, Percent_Identity=30.4843304843305, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI4504509, Length=351, Percent_Identity=30.4843304843305, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI4504507, Length=353, Percent_Identity=30.8781869688385, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI19923621, Length=352, Percent_Identity=29.8295454545455, Blast_Score=86, Evalue=7e-17,
Organism=Escherichia coli, GI87081792, Length=337, Percent_Identity=26.7062314540059, Blast_Score=111, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17570557, Length=335, Percent_Identity=25.9701492537313, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI193211272, Length=340, Percent_Identity=25.5882352941176, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17509709, Length=290, Percent_Identity=25.1724137931034, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6321437, Length=354, Percent_Identity=31.9209039548023, Blast_Score=146, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.1.1.145; =5.3.3.1 [H]

Molecular weight: Translated: 42651; Mature: 42651

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLITELNTKMTLTVTECDMTDNPVVLQADTTPTGRPDNLAQAGFDPREQAALYALAAKV
CCCEEECCCEEEEEEEECCCCCCCEEEEECCCCCCCCCCHHHCCCCHHHHHHHHHHHHHH
SHAFVTGAGGFLGKAICLRLVAAGIKVTGFARGHYPELEALGVTMVQGDLAYPEQVKQAM
HHHEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHCCEEEEECCCCCHHHHHHHH
QGCDIVFHVASKAGVWGDRDSYFCPNVKGAANVIAACKAFKISKLVYTSTPSVTFAGEDE
HCCEEEEEEHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCEEECCCCC
SGIDESTPYASRFLNYYAHSKAIAEKMMLDANQASITASAYALKTVALRPHLIWGPNDPH
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEHHHHHHEEEEECCEEEECCCCCC
LVPRVLARGRLGKLKLVGREDKLVDTIYIDNAAYAHILAAVELCQVSPKCQGKAYFVSND
HHHHHHHCCCCCEEEEECCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCCEEEEECCC
EPVTMAKMLNLILACDGLPPVTARVPQTLAYVAGAVLETAYRLLNKQEEPIMTRFVAKQL
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
SCSHYFDISAAKRDFGYHALVSIEEGMKRLKASL
CCCCEEECCHHHHCCCCEEEEEHHHHHHHHHHCC
>Mature Secondary Structure
MHLITELNTKMTLTVTECDMTDNPVVLQADTTPTGRPDNLAQAGFDPREQAALYALAAKV
CCCEEECCCEEEEEEEECCCCCCCEEEEECCCCCCCCCCHHHCCCCHHHHHHHHHHHHHH
SHAFVTGAGGFLGKAICLRLVAAGIKVTGFARGHYPELEALGVTMVQGDLAYPEQVKQAM
HHHEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHCCEEEEECCCCCHHHHHHHH
QGCDIVFHVASKAGVWGDRDSYFCPNVKGAANVIAACKAFKISKLVYTSTPSVTFAGEDE
HCCEEEEEEHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCEEECCCCC
SGIDESTPYASRFLNYYAHSKAIAEKMMLDANQASITASAYALKTVALRPHLIWGPNDPH
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEHHHHHHEEEEECCEEEECCCCCC
LVPRVLARGRLGKLKLVGREDKLVDTIYIDNAAYAHILAAVELCQVSPKCQGKAYFVSND
HHHHHHHCCCCCEEEEECCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCCEEEEECCC
EPVTMAKMLNLILACDGLPPVTARVPQTLAYVAGAVLETAYRLLNKQEEPIMTRFVAKQL
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
SCSHYFDISAAKRDFGYHALVSIEEGMKRLKASL
CCCCEEECCHHHHCCCCEEEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]